Stenocereus thurberi is an endemic species in northwestern Mexico. It produces colorful fruits called pitayas that have an edible pulp. They have phytochemical compounds associated with biological activities. Ultrafiltration is a widely used method for the clarification of fruit juices and the recovery of phytochemicals. However, its effect has not been extensively studied in extracts. Therefore, the objective of this work is to study the effect of the ultrafiltration of pitaya extract (Stenocereus thurberi) on its phytochemical content, antioxidant capacity, and identification of phenolic compounds by UPLC-DAD-MS, providing greater knowledge about the pitaya. In this study, two extracts were analyzed, the unclarified extract (UE) and the clarified extract (CE). The antioxidant capacity was higher in the CE with 15.93 ± 0.42 mM TE/g, DPPH and 18.37 ± 0.016 mM TE/g, ABTS. The UPLC-MS analysis indicated the decrease in phenolic compounds in the CE and the presence of gallic acid and resorcinol, compounds that had not been identified in other species of Stenocereus spp. The correlation analysis indicated that all the phytochemicals present in the pitaya contribute significantly to the antioxidant capacity. The ultrafiltration process could be a viable option to improve the biological activity of the natural extracts.

At present, the development of natural polymeric microparticles is carried out to obtain release systems. Prolonged-release systems are a potential solution to avoid nitrogen (N) losses in agricultural fields. The aim of this study was to develop microspheres from wheat-gluten proteins soluble in ethanol 70% (v/v), to ascertain their characterization, and to study their potential application in agricultural fields. Soluble-protein extraction was performed with 1600 mL of ethanol 70% (v/v). Likewise, ethanolic solutions with protein concentrations of 0.5%, 1%, and 2% (w/v) are classified as non-Newtonian fluids with pseudoplastic behavior. Using the nanoprecipitation method, it was possible to develop urea-loaded microspheres with a diameter ranging from 900 nm–1.7 μm. The Fourier transform infrared spectroscopy (FTIR) test exhibited interaction through hydrogen bonds between carbonyls and amino groups from the urea and proteins. Also, the thermogravimetric analysis (TGA) test demonstrated thermal stability at 130°C. The release experiment showed that the microspheres achieved equilibrium when 88% of the urea was released. Finally, according to the empirical model of Ritger and Peppas, urea release is carried out through Fickian diffusion. We conclude that the microspheres could be applied in the fields and with this improve agricultural practices. Also, they could reduce the potential environmental pollution and developing a sustainable agriculture.

Coffee bean bagasse is one of the main by-products generated by industrial coffee production. This by-product is rich in bioactive compounds such as caffeine, caffeic and chlorogenic acid, and other phenols. The aims of this work are to optimize the extraction conditions of phenolic compounds present in coffee bean bagasse and incorporate them into stout-style craft beers, as well as to determine their effect on the phenol content and antioxidant capacity. The optimal conditions for extraction were 30% ethanol, 30 °C temperature, 17.5 mL of solvent per gram of dry sample, and 30 min of sonication time. These conditions presented a total phenol content of 115.42 ± 1.04 mg GAE/g dry weight (DW), in addition to an antioxidant capacity of 39.64 ± 2.65 μMol TE/g DW in DPPH^• and 55.51 ± 6.66 μMol TE/g DW for FRAP. Caffeine, caffeic and chlorogenic acids, and other minor compounds were quantified using HPLC-DAD. The coffee bean bagasse extracts were added to the stout craft beer and increased the concentration of phenolic compounds and antioxidant capacity of the beer. This work is the first report of the use of this by-product added to beers.

Spent coffee is rich in bioactive compounds, including chlorogenic acid, caffeic acid, and caffeine, which offer health benefits. However, digestive processes can degrade these compounds; therefore, microencapsulation within Saccharomyces cerevisiae yeast cells offers a novel method to stabilize these bioactive compounds during digestion. In fact, it is important to mention that this technique of microencapsulation in Saccharomyces cerevisiae has not been previously applied to spent coffee extract. As a result, in this study, spent coffee extract was microencapsulated in non-plasmolyzed (NPCs) and plasmolyzed (PCs) yeast cells using the spray drying method. The physicochemical properties of the extract and the microencapsulates were characterized, and the bioaccessibility of the bioactive compounds was evaluated with digestion in vitro. Encapsulation efficiency (EE) was 38.62% for NPCs and 55.78% for PCs, with loading capacities (LCs) of 126.36 and 242 g/kg, respectively (according to Equations (1) and (2)). The presence of antioxidant compounds, identified by HPLC in spent coffee, was confirmed in the microencapsulates using FTIR. In vitro digestion assays revealed higher bioaccessibility of bioactive compounds in the intestinal phase, greater than 90%, and increased antioxidant activity in beer made with plasmolyzed microcapsules (BPM). These results suggest that yeast microencapsulation effectively stabilizes the bioactive compounds of spent coffee extract, releasing them throughout the gastrointestinal tract in vitro, mainly in the intestinal phase. Thus, microencapsulated compounds could serve as functional additives with a good percentage of intestinal bioaccessibility.

México ocupa actualmente el cuarto lugar mundial en producción de sorgo. En el estado de Sonora, este cultivo es uno de los más productivos. Los mayos, un pueblo amerindio que habita en el sur del estado mexicano de Sonora, ha estado promoviendo sistemas sustentables de producción agrícola. La aplicación de bioderivados de exoesqueletos de crustáceos como el quitosano (QUI) y microorganismos promotores del crecimiento vegetal, endomicorrízicos, simbiontes, benéf icos, y antagonistas, en algunos cultivos han mostrado ser una alternativa en el proceso de germinación y mejoras de rendimientos de cosecha; sin embargo, con relación al sorgo de grano, son limitados lo estudios sobre el efecto de éstos bioderivados como el QUI y consorcios microbianos. El objetivo de la investigación consistió en evaluar en sorgo de grano, el efecto de quitosano (100 y 50%) y un consorcio microbiano (en conjunto e individual forma con QUI) contra fertilización tradicional, en la región indígena del Mayo en Basconcobe, Etchojoa, Sonora, en la inducción fenológica, rendimiento y calidad proteica del grano. Se utilizó un diseño experimental de bloques al azar, con siete tratamientos a evaluar y cinco repeticiones Como variables respuesta se midieron: tasa de germinación, clorof ila total, y en madurez f isiológica la altura de planta, longitud de panoja, rendimiento ha-1 y proteína en grano. Los resultados indican que, en rendimiento y calidad proteica del grano de sorgo, el tratamiento más ef iciente (con P < 0.05), fue con QUI 100% + consorcio microbiano utilizando al 50% una fertilización regional. Estos resultados expresan la posibilidad de utilizar el quitosano como un biofertilizante de origen marino conjuntamente con un microbiano en un sistema de producción de sorgo.