This paper addresses the potential application of flexible thermoplastic polyurethane (TPU) and poly(lactic acid) (PLA) compositions as a material for the production of antibacterial wound dressings using the Fused Filament Fabrication (FFF) 3D printing method. On the market, there are medical-grade polyurethane filaments available, but few of them have properties required for the fabrication of wound dressings, such as flexibility and antibacterial effects. Thus, research aimed at the production, characterization and modification of filaments based on different TPU/PLA compositions was conducted. The combination of mechanical (tensile, hardness), structural (FTIR), microscopic (optical and SEM), degradation (2 M HCl, 5 M NaOH, and 0.1 M CoCl₂ in 20% H₂O₂) and printability analysis allowed us to select the most promising composition for further antibacterial modification (COMP-7,5PLA). The thermal stability of the chosen antibiotic—amikacin—was tested using processing temperature and HPLC. Two routes were used for the antibacterial modification of the selected filament—post-processing modification (AMI-1) and modification during processing (AMI-2). The antibacterial activity and amikacin release profiles were studied. The postprocessing modification method turned out to be superior and suitable for wound dressing fabrication due to its proven antimicrobial activity against E. coli, P. fluorescens, S. aureus and S. epidermidis bacteria.

The skeleton is a crucial element of the motion system in the human body, whose main function is to support and protect the soft tissues. Furthermore, the elements of the skeleton act as a storage place for minerals and participate in the production of red blood cells. The bone tissue includes the craniomaxillofacial bones, ribs, and spine. There are abundant reports in the literature indicating that the amount of treatments related to bone fractures increases year by year. Nowadays, the regeneration of the bone tissue is performed by using autografts or allografts, but this treatment method possesses a few disadvantages. Therefore, new and promising methods of bone tissue regeneration are constantly being sought. They often include the implantation of tissue scaffolds, which exhibit proper mechanical and osteoconductive properties. In this paper, the preparation of polyurethane (PUR) scaffolds modified by gelatin as the reinforcing factor and hydroxyapatite as the bioactive agent was described. The unmodified and modified scaffolds were tested for their mechanical properties; morphological assessments using optical microscopy were also conducted, as was the ability for calcification using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Moreover, each type of scaffold was subjected to a degradation process in 5M NaOH and 2M HCl aqueous solutions. It was noticed that the best properties promoting the calcium phosphate deposition were obtained for scaffolds modified with 2% gelatin solution containing 5% of hydroxyapatite.

Catheter-associated urinary tract infections are the result of catheterization of the bladder. The risk of infection is directly proportional to the length of the bladder catheterization. Bacteria cells have the ability to adhere and create the biofilm on the surface of catheter materials. Bacteria’s biofilm is an extremely beneficial environment of existence for microorganisms. Microorganisms that are an integral part of the biofilm are characterized by a higher resistance to antimicrobial agents compared to the planktonic form of bacteria. The previous treatment of urinary tract infections presupposes the necessity of the oral administration of large amounts of antimicrobial agents. An alternative may be the use of antimicrobial and antiadhesion coatings on the surface of the catheters. These coatings may allow target drug delivery and contribute to dose reduction and improved drug availability. In this study, antimicrobial coatings from biodegradable polymers with clindamycin were evaluated. The antimicrobial activity of the prepared coatings against selected bacterial strains was determined. The high-performance liquid chromatography–mass spectrometry technique was applied for the analysis of the drug release from antimicrobial coatings. Graphic abstract

Bones, which are considered as hard tissues, work as scaffold for human body. They provide physical support for muscles and protect intestinal organs. Percentage of hard tissues in human body depends on age, weight, and gender. Human skeleton consists of 206 connected bones. Therefore, it is natural that the hard-tissue damage such as fractures, osteoporosis, and congenital lack of bone may appear. The innovative way of bone healing is an application of so-called tissue scaffolds. There are many synthetic polymers used in this field, but polyurethanes play a great role in this field. It is due to the possibility to control their degradation rate and to tune their surface to improve the calcification process, required for proper bone regeneration. In this article, we described the fabrication of degradable poly(ester-ether)urethane materials, having different hard-segment content (28% or 47%). PEEURs-28HS and PEEURs-47HS materials were obtained by two-step polymerization method and characterized by mechanical properties, ability to undergo oxidative degradation and surface calcification. Performed studies indicated that the PEEURs-28HS material possessed suitable properties to be proposed as a material for possible application in the bone tissue engineering.