Layered fabric systems with an electrospun nanofiber web layered onto a sandwich of woven fabric were developed to examine the feasibility of developing breathable barrier textile materials. Some parameters of nanofiber mats, including the time of electrospinning and the polymer solution concentration, were designed to change and barrier properties of specimens were compared. Air permeability, water vapor transmission, and water repellency (Bundesmann and hydrostatic pressure tests) were assessed as indications of comfort and barrier performance of different samples. These performances of layered nanofiber fabrics were compared with a well-known water repellent breathable multi-layered fabric (Gortex). Multi-layered electrospun nanofiber mats equipped fabric (MENMEF) showed better performance in windproof property than Gortex fabric. Also, water vapor permeability of MENMEF was in a range of normal woven sport and work clothing. Comparisons of barrier properties of MENMEF and the currently available PTFE coated materials showed that, those properties could be achieved by layered fabric systems with electrospun nanofiber mats.