Disease and pest detection of grape foliage is essential for grape yield and quality. RGB image (RGBI), multispectral image (MSI), and thermal infrared image (TIRI) are widely used in the health detection of plants. In this study, we collected three types of grape foliage images with six common classes (anthracnose, downy mildew, leafhopper, mites, viral disease, and healthy) in the field. ShuffleNet V2 was used to build up detection models. According to the accuracy of RGBI, MSI, TIRI, and multi-source data concatenation (MDC) models, and a multi-source data fusion (MDF) decision-making method was proposed for improving the detection performance for grape foliage, aiming to enhance the decision-making for RGBI of grape foliage by fusing the MSI and TIRI. The results showed that 40% of the incorrect detection outputs were rectified using the MDF decision-making method. The overall accuracy of MDF model was 96.05%, which had improvements of 2.64%, 13.65%, and 27.79%, compared with the RGBI, MSI, and TIRI models using label smoothing, respectively. In addition, the MDF model was based on the lightweight network with 3.785 M total parameters and 0.362 G multiply-accumulate operations, which could be highly portable and easy to be applied.

The breeding of high-quality, high-yield, and disease-resistant varieties is closely related to food security. The investigation of breeding results relies on the evaluation of seed phenotype, which is a key step in the process of breeding. In the global digitalization trend, digital technology based on optical sensors can perform the digitization of seed phenotype in a non-contact, high throughput way, thus significantly improving breeding efficiency. This paper provides a comprehensive overview of the principles, characteristics, data processing methods, and bottlenecks associated with three digital technique types based on optical sensors: spectroscopy, digital imaging, and three-dimensional (3D) reconstruction techniques. In addition, the applicability and adaptability of digital techniques based on the optical sensors of maize seed phenotype traits, namely external visible phenotype (EVP) and internal invisible phenotype (IIP), are investigated. Furthermore, trends in future equipment, platform, phenotype data, and processing algorithms are discussed. This review offers conceptual and practical support for seed phenotype digitization based on optical sensors, which will provide reference and guidance for future research. The digital techniques based on optical sensors can perform non-contact and high-throughput seed phenotype evaluation. Due to the distinct characteristics of optical sensors, matching suitable digital techniques according to seed phenotype traits can greatly reduce resource loss, and promote the efficiency of seed evaluation as well as breeding decision-making. Future research in phenotype equipment and platform, phenotype data, and processing algorithms will make digital techniques better meet the demands of seed phenotype evaluation, and promote automatic, integrated, and intelligent evaluation of seed phenotype, further helping to lessen the gap between digital techniques and seed phenotyping.

Accurate monitoring of rice phenology is critical for crop management, cultivars breeding, and yield estimating. Previously, research for phenology detection relied on time-series data and orthomosaic and manually plotted regions, which are difficult to automate. This study presented a novel approach for extracting and mapping phenological traits directly from the unmanned aerial vehicle (UAV) photograph sequence. First, a multi-stage rice field segmentation dataset containing four growth stages and 2600 images, namely PaddySeg, was built. Moreover, an efficient Ghost Bilateral Network (GBiNet) was proposed to generate trait masks. To locate the trait of each pixel, we introduced direct geo-locating (DGL) and incremental sparse sampling (ISS) techniques to eliminate redundant computation. According to the results on PaddySeg, the proposed GBiNet with 91.50% mean-Intersection-over-Union (mIoU) and 41 frames-per-second (FPS) speed outperformed the baseline model (90.95%, 36 FPS), while the fastest GBiNet_t reached 62 FPS which was 1.7 times faster than the baseline model, BiSeNetV2. Additionally, the measured average DGL deviation was less than 1% of the relative height. Finally, the mapping of rice phenology was achieved by interpolation on trait value–location pairs. The proposed approach demonstrated great potential for automatic rice phenology stage surveying and mapping.

Efficient and quick yield prediction is of great significance for ensuring world food security and crop breeding research. The rapid development of unmanned aerial vehicle (UAV) technology makes it more timely and accurate to monitor crops by remote sensing. The objective of this study was to explore the method of developing a novel yield index (YI) with wide adaptability for yield prediction by fusing vegetation indices (VIs), color indices (CIs), and texture indices (TIs) from UAV-based imagery. Six field experiments with 24 varieties of rice and 21 fertilization methods were carried out in three experimental stations in 2019 and 2020. The multispectral and RGB images of the rice canopy collected by the UAV platform were used to rebuild six new VIs and TIs. The performance of VI-based YI (MAPE = 13.98%) developed by quadratic nonlinear regression at the maturity stage was better than other stages, and outperformed that of CI-based (MAPE = 22.21%) and TI-based (MAPE = 18.60%). Then six VIs, six CIs, and six TIs were fused to build YI by multiple linear regression and random forest models. Compared with heading stage (R² = 0.78, MAPE = 9.72%) and all stage (R² = 0.59, MAPE = 22.21%), the best performance of YI was developed by random forest with fusing VIs + CIs + TIs at maturity stage (R² = 0.84, MAPE = 7.86%). Our findings suggest that the novel YI proposed in this study has great potential in crop yield monitoring.

In order to rationally apply potassium fertilizer, it is very important to realize the rapid and accurate evaluation of soil available potassium (K). Conventional methods are time-consuming, consumables-consuming and laborious. A high-efficiency method was proposed in this study to meet the demand for rapid evaluation, including rapid extraction, uniform evaporation and LIBS detection. To shorten the extraction time, we increased the oscillation frequency and removed the operation of dry filtration. Compared with the conventional extraction method of the Chinese national standard (CNS), the extraction time was reduced from 30 min to 2 min. In addition, we developed a uniform evaporation method for liquid–solid transformation on the batch-detection fixed area aluminum substrate. This method reduced the moisture interference. At the same time, increasing the liquid viscosity and restricting the liquid area and shape could reduce the coffee ring effect (CRE). The determination coefficient of the calibration curve by our method was 0.99, and the limit of quantitation reached 0.8 mg/kg. Real soil samples were taken as validation, and the average relative error between our method and the CNS method was 3.58%. The results indicate that our method combined with LIBS technology could provide a fast and accurate evaluation of soil available K.

Ginkgo biloba L. is a rare dioecious species that is valued for its diverse applications and is cultivated globally. This study aimed to develop a rapid and effective method for determining the sex of a Ginkgo biloba. Green and yellow leaves representing annual growth stages were scanned with a hyperspectral imager, and classification models for RGB images, spectral features, and a fusion of spectral and image features were established. Initially, a ResNet101 model classified the RGB dataset using the proportional scaling–background expansion preprocessing method, achieving an accuracy of 90.27%. Further, machine learning algorithms like support vector machine (SVM), linear discriminant analysis (LDA), and subspace discriminant analysis (SDA) were applied. Optimal results were achieved with SVM and SDA in the green leaf stage and LDA in the yellow leaf stage, with prediction accuracies of 87.35% and 98.85%, respectively. To fully utilize the optimal model, a two-stage Period-Predetermined (PP) method was proposed, and a fusion dataset was built using the spectral and image features. The overall accuracy for the prediction set was as high as 96.30%. This is the first study to establish a standard technique framework for Ginkgo sex classification using hyperspectral imaging, offering an efficient tool for industrial and ecological applications and the potential for classifying other dioecious plants.