Model-based 3D/2D image registration using single-plane fluoroscopy is a common setup to determine knee joint kinematics, owing to its markerless aspect. However, the approach was subjected to lower accuracies in the determination of out-of-plane motion components. Introducing additional kinematic constraints with an appropriate anatomical representation may help ameliorate the reduced accuracy of single-plane image registration. Therefore, this study aimed to develop and evaluate a multibody model-based tracking (MbMBT) scheme, embedding a personalized kinematic model of the tibiofemoral joint for the measurement of tibiofemoral kinematics. The kinematic model was consisted of three ligaments and an articular contact mechanism. The knee joint activities in six volunteers during isolated knee flexion, lunging, and sit-to-stand motions were recorded with a biplane X-ray imaging system. The tibiofemoral kinematics determined with the MbMBT and mediolateral view fluoroscopic images were compared against those determined using biplane fluoroscopic images. The MbMBT was demonstrated to yield tibiofemoral kinematics with precision values in the range from 0.1 mm to 1.1 mm for translations and from 0.2° to 1.3° for rotations. The constraints provided by the kinematic model were shown to effectively amend the nonphysiological tibiofemoral motion and not compromise the image registration accuracy with the proposed MbMBT scheme.