Incomplete and inadequate removal of endodontic biofilm during root canal treatment often leads to the clinical failure. Over the past decade, biofilm eradication techniques, such as sonication of irrigant solutions, ultrasonic and laser devices have been investigated in laboratory settings. This review aimed to give an overview of endodontic biofilm cultivation methods described in papers which investigated sonic-, ultrasonic- and Er:Yag laser-assisted biofilm removal techniques. Furthermore, the effectiveness of these removal techniques was discussed, as well as methods used for the evaluation of the cleaning efficacy. In general, laser assisted agitation, as well as ultrasonic and sonic activation of the irrigants provide a more efficient biofilm removal compared to conventional irrigation conducted by syringe/needle. The choice of irrigant is an important factor for reducing the bacterial contamination inside the root canal, with water and saline being the least effective. Due to heterogeneity in methods among the reviewed studies, it is difficult to compare sonic-, ultrasonic- and Er:Yag laser-assisted techniques among each other and give recommendations for the most efficient method in biofilm removal. Future studies should standardize the methodology regarding biofilm cultivation and cleaning methods, root canals with complex morphology should be introduced in research, with the aim of simulating the clinical scenario more closely.

Objective: To evaluate apical extrusion during a novel erbium-doped yttrium aluminum garnet (Er:YAG) laser-activated irrigation (LAI) modality. Background data: A novel double-pulse Er:YAG modality (AutoSWEEPS) was introduced recently, replacing a single laser pulse with two micropulses that are separated by a varying time delay (which is continuously “swept” between 300 and 600 μsec). Although the proposed method demonstrated increased efficacy, no data were yet available on extrusion. Methods: The extrusion was evaluated on simulated canals (n = 6) using particle imaging velocimetry. In the first two groups, the irrigation device was a syringe coupled to either a 30-G open-ended or side-vented needle, with flow rates of 1, 2, 5, and 15 mL/min. In the second two groups, irrigant activation was performed with an Er:YAG laser, using either a super-short pulse (SSP) or AutoSWEEPS modality. The pulse energies were 5, 10, 20, 30, and 40 mJ and the frequency was 10 Hz. Results: The measured extrusion was most prominent during the open-ended needle irrigation, followed by the vented needle irrigation. Compared with the conventional needle irrigation (CNI), all the studied LAI modalities resulted in ∼3–20 times less extrusion. The AutoSWEEPS modality induced the smallest extrusion rate, which was always <1.5 mm³/sec and was also independent of the laser energy. Conclusions: Within the limitations of the study, our results demonstrate that the SSP and AutoSWEEPS laser-assisted irrigation methods exhibited less extrusion in comparison with CNI methods.