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Second-generation biofuel crop miscanthus is one of the most promising plants tested for phytomanagement of contaminated sites. In this preliminary pot case study, the most used hybrid Miscanthus x giganteus was cultivated in three different real contaminated soils: agricultural soil contaminated with Cd; post-military soil slightly contaminated with Zn, Pb and Cd; and soil contaminated by petroleum industry with metals and hydrocarbons. The stress response of plants and soil microbial communities was monitored to receive data that are important for successful phytomanagement application. With metals only, the plant grew well, and chlorophyll fluorescence measurement proved their good vitality. Changes in leaf anatomy (leaf thickness and sclerenchyma cells area) were additionally determined in post-military soil compared to agricultural. On the contrary, in petroleum-contaminated soil, the biomass yield was too reduced and also physiological parameters were significantly decreased. The response of microbial communities also differed. In agricultural soil, no microbial stress was determined. In post-military soil, it became reduced during the experiment, and in petroleum contamination, it increased year-on-year. It could be concluded that miscanthus is suitable for cultivation in metals contaminated soils with potential for microbial communities support, but in soil contaminated by the petroleum industry, its application did not seem meaningful.
Diana Nebeská; Hana Auer Malinská; Anna Erol; Valentina Pidlisnyuk; Pavel Kuráň; Andrea Medžová; Martin Smaha; Josef Trögl. Stress Response of Miscanthus Plants and Soil Microbial Communities: A Case Study in Metals and Hydrocarbons Contaminated Soils. Applied Sciences 2021, 11, 1866 .
AMA StyleDiana Nebeská, Hana Auer Malinská, Anna Erol, Valentina Pidlisnyuk, Pavel Kuráň, Andrea Medžová, Martin Smaha, Josef Trögl. Stress Response of Miscanthus Plants and Soil Microbial Communities: A Case Study in Metals and Hydrocarbons Contaminated Soils. Applied Sciences. 2021; 11 (4):1866.
Chicago/Turabian StyleDiana Nebeská; Hana Auer Malinská; Anna Erol; Valentina Pidlisnyuk; Pavel Kuráň; Andrea Medžová; Martin Smaha; Josef Trögl. 2021. "Stress Response of Miscanthus Plants and Soil Microbial Communities: A Case Study in Metals and Hydrocarbons Contaminated Soils." Applied Sciences 11, no. 4: 1866.
Bioenergy crops such as Miscanthus × giganteus are foreseeable as an alternative source to replace fossil fuel and reduce greenhouse gas emissions. They are also assessed as an environment-friendly solution for polluted, marginal and low-quality agricultural soils. Several studies had been launched on soil organic carbon sequestration potentials of miscanthus culture along with its impacts on restoring soil functionality, most of which focus on the long-term basis of the plant’s cultivation. Nevertheless, information concerning the short term impacts as well as the situation in Czechia is still scarce. In this context, a field experiment was launched in 2017 in a poor-quality agricultural land in the city of Chomutov (North-Western Czechia) to compare the impacts of the perennial C4 miscanthus with an annual C3 forage crop (wheat) on the soil carbon stocks as well as enhancing its functionality. Results through the 0–30 cm soil profile examination showed that miscanthus plants played a role in improving the studied soil physico-chemical (bulk density and soil organic carbon concentrations) and biological (Phospholipid fatty acids stress indicator, basal respiration and fluorescein diacetate hydrolytic activity) parameters. The naturally occurring δ13C concentrations were used to evaluate the direct plant contribution to the total soil organic carbon (SOC) stocks and revealed considerable miscanthus contribution all over the detected soil layers (1.98 ± 0.21 Mg C. ha−1 yr−1) after only 3 growing seasons. It is thus suggested that the C4 perennial miscanthus possess remarkable prospects for SOC sequestration and restoring degraded lands.
Karim Suhail Al Souki; Hana Burdová; Jakub Trubač; Jiří Štojdl; Pavel Kuráň; Sylvie Kříženecká; Iva Machová; Karel Kubát; Jan Popelka; Hana Auer Malinská; Diana Nebeská; Sergej Ust’Ak; Roman Honzík; Josef Trögl. Enhanced Carbon Sequestration in Marginal Land Upon Shift towards Perennial C4Miscanthus × giganteus: A Case Study in North-Western Czechia. Agronomy 2021, 11, 293 .
AMA StyleKarim Suhail Al Souki, Hana Burdová, Jakub Trubač, Jiří Štojdl, Pavel Kuráň, Sylvie Kříženecká, Iva Machová, Karel Kubát, Jan Popelka, Hana Auer Malinská, Diana Nebeská, Sergej Ust’Ak, Roman Honzík, Josef Trögl. Enhanced Carbon Sequestration in Marginal Land Upon Shift towards Perennial C4Miscanthus × giganteus: A Case Study in North-Western Czechia. Agronomy. 2021; 11 (2):293.
Chicago/Turabian StyleKarim Suhail Al Souki; Hana Burdová; Jakub Trubač; Jiří Štojdl; Pavel Kuráň; Sylvie Kříženecká; Iva Machová; Karel Kubát; Jan Popelka; Hana Auer Malinská; Diana Nebeská; Sergej Ust’Ak; Roman Honzík; Josef Trögl. 2021. "Enhanced Carbon Sequestration in Marginal Land Upon Shift towards Perennial C4Miscanthus × giganteus: A Case Study in North-Western Czechia." Agronomy 11, no. 2: 293.
Glucose belongs among the most important substances in both physiology and industry. Current food and biotechnology praxis emphasizes its on-line continuous monitoring and regulation. These provoke increasing demand for systems, which enable fast detection and regulation of deviations from desired glucose concentration. We demonstrated control of glucose concentration by feedback regulation equipped with in situ optical fiber glucose sensor. The sensitive layer of the sensor comprises oxygen-dependent ruthenium complex and preimmobilized glucose oxidase both entrapped in organic–inorganic polymer ORMOCER®. The sensor was placed in the laboratory bioreactor (volume 5 L) to demonstrate both regulations: the control of low levels of glucose concentrations (0.4 and 0.1 mM) and maintenance of the glucose concentration (between 2 and 3.5 mM) during stationary phase of cultivation of Saccharomyces cerevisiae. Response times did not exceed 6 min (average 4 min) with average deviation of 4%. Due to these regulation characteristics together with durable and long-lasting (≥2 month) sensitive layer, this feedback regulation system might find applications in various biotechnological processes such as production of low glucose content beverages.
Lucie Koštejnová; Jakub Ondráček; Petra Majerová; Martin Koštejn; Gabriela Kuncová; Josef Trögl. Cultivation of Saccharomyces cerevisiae with Feedback Regulation of Glucose Concentration Controlled by Optical Fiber Glucose Sensor. Sensors 2021, 21, 565 .
AMA StyleLucie Koštejnová, Jakub Ondráček, Petra Majerová, Martin Koštejn, Gabriela Kuncová, Josef Trögl. Cultivation of Saccharomyces cerevisiae with Feedback Regulation of Glucose Concentration Controlled by Optical Fiber Glucose Sensor. Sensors. 2021; 21 (2):565.
Chicago/Turabian StyleLucie Koštejnová; Jakub Ondráček; Petra Majerová; Martin Koštejn; Gabriela Kuncová; Josef Trögl. 2021. "Cultivation of Saccharomyces cerevisiae with Feedback Regulation of Glucose Concentration Controlled by Optical Fiber Glucose Sensor." Sensors 21, no. 2: 565.
J. Trögl; I. Matušíková. Conference applied natural sciences 2019. International Journal of Environmental Science and Technology 2021, 18, 1 -1.
AMA StyleJ. Trögl, I. Matušíková. Conference applied natural sciences 2019. International Journal of Environmental Science and Technology. 2021; 18 (1):1-1.
Chicago/Turabian StyleJ. Trögl; I. Matušíková. 2021. "Conference applied natural sciences 2019." International Journal of Environmental Science and Technology 18, no. 1: 1-1.
Use of plant growth-promoting bacteria (PGPB) for cultivation of the biofuel crop Miscanthus × giganteus (Mxg) in post-military and post-mining sites is a promising approach for the bioremediation of soils contaminated by metals. In the present study, PGPB were isolated from contaminated soil and screened for tolerance against abiotic stresses caused by salinity, pH, temperature, and lead (Pb). Selected strains were further assessed and screened for plant growth-promoting attributes. The isolate showing the most potential, Bacillus altitudinis KP-14, was tested for enhancement of Mxg growth in contaminated soil under greenhouse conditions. It was found to be highly tolerant to diverse abiotic stresses, exhibiting tolerance to salinity (0–15%), pH (4–8), temperature (4–50 °C), and Pb (up to 1200 ppm). The association of B. altitudinis KP-14 with Mxg resulted in a significant (p ≤ 0.001) impact on biomass enhancement: the total shoot and dry root weights were significantly enhanced by 77.7% and 55.5%, respectively. The significant enhancement of Mxg biomass parameters by application of B. altitudinis KP-14 strongly supports the use of this strain as a biofertilizer for the improvement of plant growth in metal-contaminated soils.
Kumar Pranaw; Valentina Pidlisnyuk; Josef Trögl; Hana Malinská. Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus Altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil. Biology 2020, 9, 305 .
AMA StyleKumar Pranaw, Valentina Pidlisnyuk, Josef Trögl, Hana Malinská. Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus Altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil. Biology. 2020; 9 (9):305.
Chicago/Turabian StyleKumar Pranaw; Valentina Pidlisnyuk; Josef Trögl; Hana Malinská. 2020. "Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus Altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil." Biology 9, no. 9: 305.
Valentina Pidlisnyuk; Larry Erickson; Tatyana Stefanovska; Ganga Hettiarachchi; Lawrence Davis; Josef Trögl; Pavlo Shapoval. Response to Grygar (2020) comments on “Potential phytomanagement of military polluted sites and biomass production using biofuel crop miscanthus x giganteus”- Pidlisnyuk et al. (2019). Environmental pollution, 261: 113038. Environmental Pollution 2020, 272, 115037 .
AMA StyleValentina Pidlisnyuk, Larry Erickson, Tatyana Stefanovska, Ganga Hettiarachchi, Lawrence Davis, Josef Trögl, Pavlo Shapoval. Response to Grygar (2020) comments on “Potential phytomanagement of military polluted sites and biomass production using biofuel crop miscanthus x giganteus”- Pidlisnyuk et al. (2019). Environmental pollution, 261: 113038. Environmental Pollution. 2020; 272 ():115037.
Chicago/Turabian StyleValentina Pidlisnyuk; Larry Erickson; Tatyana Stefanovska; Ganga Hettiarachchi; Lawrence Davis; Josef Trögl; Pavlo Shapoval. 2020. "Response to Grygar (2020) comments on “Potential phytomanagement of military polluted sites and biomass production using biofuel crop miscanthus x giganteus”- Pidlisnyuk et al. (2019). Environmental pollution, 261: 113038." Environmental Pollution 272, no. : 115037.
In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after
Jakub Zajíc; Steven Ripp; Josef Trögl; Gabriela Kuncová; Marie Pospíšilová. Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements. Sensors 2020, 20, 3237 .
AMA StyleJakub Zajíc, Steven Ripp, Josef Trögl, Gabriela Kuncová, Marie Pospíšilová. Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements. Sensors. 2020; 20 (11):3237.
Chicago/Turabian StyleJakub Zajíc; Steven Ripp; Josef Trögl; Gabriela Kuncová; Marie Pospíšilová. 2020. "Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements." Sensors 20, no. 11: 3237.
Mercury is a ubiquitous environmental pollutant of dominantly anthropogenic origin. A critical concern for human health is the introduction of mercury to the food chain; therefore, monitoring of mercury levels in agricultural soil is essential. Unfortunately, the total mercury content is not sufficiently informative as mercury can be present in different forms with variable bioavailability. Since 1990, the use of bioreporters has been investigated for assessment of the bioavailability of pollutants; however, real contaminated soils have rarely been used in these studies. In this work, a bioassay with whole-cell bacterial bioreporter Escherichia coli ARL1 was used for estimation of bioavailable concentration of mercury in 11 soil samples. The bioreporter emits bioluminescence in the presence of Hg(II). Four different pretreatments of soil samples prior to the bioassay were tested. Among them, laccase mediated extraction was found to be the most suitable over water extraction, alkaline extraction, and direct use of water-soil suspensions. Nevertheless, effect of the matrix on bioreporter signal was found to be severe and not possible to be completely eliminated by the method of standard addition. In order to elucidate the matrix role, influences of humic acid and selected metal ions present in soil on the bioreporter signal were tested separately in laboratory solutions. Humic acids were found to have a positive effect on the bioreporter growth, but a negative effect on the measured bioluminescence, likely due to shading and Hg binding resulting in decreased bioavailability. Each of the tested metal ions solutions affected the bioluminescence signal differently; cobalt (II) positively, iron (III) negatively, and the effects of iron (II) and nickel (II) were dependent on their concentrations. In conclusion, the information on bioavailable mercury estimated by bioreporter E. coli ARL1 is valuable, but the results must be interpreted with caution. The route to functional bioavailability bioassay remains long.
Irena Brányiková; Simona Lucáková; Gabriela Kuncová; Josef Trögl; Václav Synek; Jan Rohovec; Tomáš Navrátil. Estimation of Hg(II) in Soil Samples by Bioluminescent Bacterial Bioreporter E. coli ARL1, and the Effect of Humic Acids and Metal Ions on the Biosensor Performance. Sensors 2020, 20, 3138 .
AMA StyleIrena Brányiková, Simona Lucáková, Gabriela Kuncová, Josef Trögl, Václav Synek, Jan Rohovec, Tomáš Navrátil. Estimation of Hg(II) in Soil Samples by Bioluminescent Bacterial Bioreporter E. coli ARL1, and the Effect of Humic Acids and Metal Ions on the Biosensor Performance. Sensors. 2020; 20 (11):3138.
Chicago/Turabian StyleIrena Brányiková; Simona Lucáková; Gabriela Kuncová; Josef Trögl; Václav Synek; Jan Rohovec; Tomáš Navrátil. 2020. "Estimation of Hg(II) in Soil Samples by Bioluminescent Bacterial Bioreporter E. coli ARL1, and the Effect of Humic Acids and Metal Ions on the Biosensor Performance." Sensors 20, no. 11: 3138.
Miscanthus x giganteus (Mxg) is a promising second-generation biofuel crop with high production of energetic biomass. Our aim was to determine the level of plant stress of Mxg grown in poor quality soils using non-invasive physiological parameters and to test whether the stress could be reduced by application of plant growth regulators (PGRs). Plant fitness was quantified by measuring of leaf fluorescence using 24 indexes to select the most suitable fluorescence indicators for quantification of this type of abiotic stress. Simultaneously, visible stress signs were observed on stems and leaves and differences in variants were revealed also by microscopy of leaf sections. Leaf fluorescence analysis, visual observation and changes of leaf anatomy revealed significant stress in all studied subjects compared to those cultivated in good quality soil. Besides commonly used Fv/Fm (potential photosynthetic efficiency) and P.I. (performance index), which showed very low sensitivity, we suggest other fluorescence parameters (like dissipation, DIo/RC) for revealing finer differences. We can conclude that measurement of leaf fluorescence is a suitable method for revealing stress affecting Mxg in poor soils. However, none of investigated parameters proved significant positive effect of PGRs on stress reduction. Therefore, direct improvement of soil quality by fertilization should be considered for stress reduction and improving the biomass quality in this type of soils.
Hana Malinská; Valentina Pidlisnyuk; Diana Nebeská; Anna Erol; Andrea Medžová; Josef Trögl. Physiological Response of Miscanthus x giganteus to Plant Growth Regulators in Nutritionally Poor Soil. Plants 2020, 9, 194 .
AMA StyleHana Malinská, Valentina Pidlisnyuk, Diana Nebeská, Anna Erol, Andrea Medžová, Josef Trögl. Physiological Response of Miscanthus x giganteus to Plant Growth Regulators in Nutritionally Poor Soil. Plants. 2020; 9 (2):194.
Chicago/Turabian StyleHana Malinská; Valentina Pidlisnyuk; Diana Nebeská; Anna Erol; Andrea Medžová; Josef Trögl. 2020. "Physiological Response of Miscanthus x giganteus to Plant Growth Regulators in Nutritionally Poor Soil." Plants 9, no. 2: 194.
A whole-cell optical fiber sensor of toluene was prepared by the adsorption of Pseudomonas putida TVA8, the bacterial strain producing light in contact with toluene, in the form of biofilm. Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane (APTES) treatment on the wider end of tapered quartz optical fiber element (OFE). Repeatability of preparation and response of the active part of the biosensor was demonstrated in five trials with different OFE geometries in which the bioluminescence (BL) was induced with toluene solution (26.5 mg L −1 ) for 2 weeks. Longevity of inductions of the biosensor was shown in over 135 days trial, when the biosensor was repetitively induced with toluene solution (26.5 mg L −1 ) 68 times. The intensities of BL gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of BL induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor is plausible for multiple and fast online monitoring of the presence of a pollutant in remote localities but not its concentration.
Jakub Zajic; Steven Ripp; Josef Trogl; Gabriela Kuncova; Marie Pospisilova. Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements. 2019 IEEE International Conference on Sensors and Nanotechnology 2019, 1 -4.
AMA StyleJakub Zajic, Steven Ripp, Josef Trogl, Gabriela Kuncova, Marie Pospisilova. Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements. 2019 IEEE International Conference on Sensors and Nanotechnology. 2019; ():1-4.
Chicago/Turabian StyleJakub Zajic; Steven Ripp; Josef Trogl; Gabriela Kuncova; Marie Pospisilova. 2019. "Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements." 2019 IEEE International Conference on Sensors and Nanotechnology , no. : 1-4.
Fertilization is a key factor for sustaining productivity in agroecosystems. A long-term experiment in cambisol following periodical application of several types of fertilization has been running at the experimental site since 1954. In this study, we determined the impact of applied inorganic and/or organic fertilizers on the activity of soil enzymes and on the structure of microorganisms at depths of 0–30 cm and 30–60 cm. Single-factor comparison showed that use of inorganic and/or organic fertilizer had an insignificant effect on the activities of soil enzymes (at depths 0–30 cm and 30–60 cm) and also on the structure of microbial communities at both depths studied. Only soil respirations exhibited stimulation by combined fertilization. The results, irrespective of sampling depth (0–60 cm), showed that application of combined organic and inorganic fertilization stimulated the activity of glucosidases and use of inorganic fertilizer inhibited the activity of arylsulphatases. Respirations were stimulated by application of organic fertilizer and combined fertilization. Nevertheless, principal component analyses, which calculate with multidimensional data, revealed differences in samples treated by sole mineral fertilizer compared to other variants, especially in the lower layer. In general, our results indicate that use of combined fertilization may improve biological characteristics in deeper parts of soil profile and possibly increase biological activity in agroecosystems.
Ladislav Holík; Lukáš Hlisnikovský; Roman Honzík; Josef Trögl; Hana Burdová; Jan Popelka. Soil Microbial Communities and Enzyme Activities after Long-Term Application of Inorganic and Organic Fertilizers at Different Depths of the Soil Profile. Sustainability 2019, 11, 3251 .
AMA StyleLadislav Holík, Lukáš Hlisnikovský, Roman Honzík, Josef Trögl, Hana Burdová, Jan Popelka. Soil Microbial Communities and Enzyme Activities after Long-Term Application of Inorganic and Organic Fertilizers at Different Depths of the Soil Profile. Sustainability. 2019; 11 (12):3251.
Chicago/Turabian StyleLadislav Holík; Lukáš Hlisnikovský; Roman Honzík; Josef Trögl; Hana Burdová; Jan Popelka. 2019. "Soil Microbial Communities and Enzyme Activities after Long-Term Application of Inorganic and Organic Fertilizers at Different Depths of the Soil Profile." Sustainability 11, no. 12: 3251.
This study aims to summarize results on potential phytomanagement of two metal(loid)-polluted military soils using Miscanthus x giganteus. Such an option was tested during 2-year pot experiments with soils taken from former military sites in Sliač, Slovakia and Kamenetz-Podilsky, Ukraine. The following elements were considered: As, Cu, Fe, Mn, Pb, Sr, Ti, Zn and Zr. M. x giganteus showed good growth at both military soils with slightly higher maximum shoot lengths in the second year of vegetation. Based on Principal Component Analysis similarities of metal(loid) uptake by roots, stems and leaves were summarized. Major part of the elements remained in M. x giganteus roots and rather limited amounts moved to the aerial parts. Levels taken up decreased in the second vegetation year. Dynamics of foliar metal(loid) concentrations divided the elements in two groups: essential elements required for metabolism (Fe, Mn, Cu, and Zn) and non-essential elements without any known metabolic need (As, Sr, Ti, and Zr). Fe, Mn, Ti and Sr showed similar S-shaped uptake curve in terms of foliar concentrations (likely due to dilution in growing biomass), while Cu exhibited a clear peak mid-season. Behavior of Zn was in between. Foliar Zr and As concentrations were below detection limit. The results illustrated a good potential of M. x giganteus for safely growing on metal-polluted soils taken from both military localities.
Valentina Pidlisnyuk; Larry Erickson; Tatyana Stefanovska; Jan Popelka; Ganga Hettiarachchi; Lawrence Davis; Josef Trögl. Potential phytomanagement of military polluted sites and biomass production using biofuel crop miscanthus x giganteus. Environmental Pollution 2019, 249, 330 -337.
AMA StyleValentina Pidlisnyuk, Larry Erickson, Tatyana Stefanovska, Jan Popelka, Ganga Hettiarachchi, Lawrence Davis, Josef Trögl. Potential phytomanagement of military polluted sites and biomass production using biofuel crop miscanthus x giganteus. Environmental Pollution. 2019; 249 ():330-337.
Chicago/Turabian StyleValentina Pidlisnyuk; Larry Erickson; Tatyana Stefanovska; Jan Popelka; Ganga Hettiarachchi; Lawrence Davis; Josef Trögl. 2019. "Potential phytomanagement of military polluted sites and biomass production using biofuel crop miscanthus x giganteus." Environmental Pollution 249, no. : 330-337.
This article presents the development of selected heavy metals contained in river sediments at selected sites along the Bílina River. It describes the situation before the large flood in 2013, the situation just after that flood, the situation approximately 1 year after the flood, and 4 years after the flood. The contents of selected heavy metals from industrial pollution originating from transport and industry located along the river flow were monitored. Since the situation in the river before the flood was also known, it was possible to assess the impact of the 2013 flood on the distribution of heavy metals in the settled sediments. The results obtained show that the heavy metal content has changed significantly. During the flood in 2013 there was an increase in the concentration of almost all the monitored elements compared to the situation before the flood and one year after the flood. This could be caused by the churning of sediments as a result of the flood. The only exception was Cd, the concentration of which did not change significantly. However, Cd is present in the sediment samples at a low concentration at the limit of quantification (LOQ), so the change may not be noticeable. In 2017, four years after the flood, an increase in the concentration of all the monitored elements was found. The highest increase in the content of the monitored elements was observed at the sites with the highest industrial pollution. Following the flood in 2014, there was a situation where no significant differences were observed in the content of metals at the individual sampling points in contrast to the values obtained in 2012, 2013, and 2017. As a result of sediment churning and re-settlement during and after the flood, the sediment became homogenized along the entire river flow. After a longer period of time that was not accompanied by floods, a significant increase in the heavy metal concentration was observed due to anthropogenic pollution. The results show that the effect of flooding is only temporal and that anthropogenic pollution is more important than the impact of floods.
Jiří Čmelík; Taťjána Brovdyová; Josef Trögl; Martin Neruda; Matěj Kadlečík; Jan Pacina; Jan Popelka; Alexander S. Sirotkin. Changes in the Content of Heavy Metals in Bílina River during 2012–2017: Effects of Flood and Industrial Inputs. Water 2019, 11, 481 .
AMA StyleJiří Čmelík, Taťjána Brovdyová, Josef Trögl, Martin Neruda, Matěj Kadlečík, Jan Pacina, Jan Popelka, Alexander S. Sirotkin. Changes in the Content of Heavy Metals in Bílina River during 2012–2017: Effects of Flood and Industrial Inputs. Water. 2019; 11 (3):481.
Chicago/Turabian StyleJiří Čmelík; Taťjána Brovdyová; Josef Trögl; Martin Neruda; Matěj Kadlečík; Jan Pacina; Jan Popelka; Alexander S. Sirotkin. 2019. "Changes in the Content of Heavy Metals in Bílina River during 2012–2017: Effects of Flood and Industrial Inputs." Water 11, no. 3: 481.
Growing a second generation energetic plant Miscanthus x giganteus (Mxg) in former military sites appears to be a promising way to use such areas with synergic phytoremediation of contaminants (organic pollutants, potential toxic metals). Effect of Mxg growth on soil microbial communities during a two-year pot experiment with real post-military soil (Sliač, Slovakia) and the effect of potential toxic metal concentration was studied by analyses of microbial activities and phospholipid fatty acids (PLFA). Year-on-year positive changes in microbial community structure and state were detected (higher ratios of G+/G− bacteria and fungal/bacterial PLFA, and a decrease of stress indicators trans/cis and cy/pre PLFA). Portion of gram-positive bacteria correlated negatively with potential toxic metal concentrations; however, the concentration of potential toxic metals was not identified as the main stress factor—the access to bioavailable nutrients was more important. Overall, Mxg growth generally exhibited a positive effect on soil microorganisms.
Diana Nebeská; Josef Trögl; Valentina Pidlisnyuk; Jan Popelka; Petra Veronesi Dáňová; Sergej Usťak; Roman Honzík. Effect of Growing Miscanthus x giganteus on Soil Microbial Communities in Post-Military Soil. Sustainability 2018, 10, 4021 .
AMA StyleDiana Nebeská, Josef Trögl, Valentina Pidlisnyuk, Jan Popelka, Petra Veronesi Dáňová, Sergej Usťak, Roman Honzík. Effect of Growing Miscanthus x giganteus on Soil Microbial Communities in Post-Military Soil. Sustainability. 2018; 10 (11):4021.
Chicago/Turabian StyleDiana Nebeská; Josef Trögl; Valentina Pidlisnyuk; Jan Popelka; Petra Veronesi Dáňová; Sergej Usťak; Roman Honzík. 2018. "Effect of Growing Miscanthus x giganteus on Soil Microbial Communities in Post-Military Soil." Sustainability 10, no. 11: 4021.
At present, there is great demand for new resistant and metabolically active strains of biodegrading bacteria capable of degrading high concentrations of petroleum pollutants. In this study, we undertook a series of pot-based biodegradation experiments on soil from a petroleum refinery lagoon heavily polluted with aliphatic hydrocarbons (81.6 ± 2.5 g·kg−1 dry weight) and metals. Periodical bioaugmentation with either a mixture of isolated degraders identified as Bacillus sp. and Ochrobactrum sp. or biostimulation with nutrient medium, singly or in combination, did not produce any significant decrease in hydrocarbons, even after 455 days. Inoculation with Gordonia rubripertincta CWB2 and Rhodococcus erythropolis S43 in iron-limited media, however, resulted in a significant decrease in hydrocarbons 45 days after bioaugmentation. These actinobacterial strains, therefore, show significant potential for bioremediation of such highly polluted soils.
Josef Trögl; Catherine Oluwakemi Esuola; Sylvie Kříženecká; Pavel Kuráň; Lenka Seidlová; Petra Veronesi-Dáňová; Jan Popelka; Olubukola Oluranti Babalola; Pavel Hrabák; Marie Czinnerová; Eva Kakosová; Alena Ševců; Dirk Tischler. Biodegradation of High Concentrations of Aliphatic Hydrocarbons in Soil from a Petroleum Refinery: Implications for Applicability of New Actinobacterial Strains. Applied Sciences 2018, 8, 1855 .
AMA StyleJosef Trögl, Catherine Oluwakemi Esuola, Sylvie Kříženecká, Pavel Kuráň, Lenka Seidlová, Petra Veronesi-Dáňová, Jan Popelka, Olubukola Oluranti Babalola, Pavel Hrabák, Marie Czinnerová, Eva Kakosová, Alena Ševců, Dirk Tischler. Biodegradation of High Concentrations of Aliphatic Hydrocarbons in Soil from a Petroleum Refinery: Implications for Applicability of New Actinobacterial Strains. Applied Sciences. 2018; 8 (10):1855.
Chicago/Turabian StyleJosef Trögl; Catherine Oluwakemi Esuola; Sylvie Kříženecká; Pavel Kuráň; Lenka Seidlová; Petra Veronesi-Dáňová; Jan Popelka; Olubukola Oluranti Babalola; Pavel Hrabák; Marie Czinnerová; Eva Kakosová; Alena Ševců; Dirk Tischler. 2018. "Biodegradation of High Concentrations of Aliphatic Hydrocarbons in Soil from a Petroleum Refinery: Implications for Applicability of New Actinobacterial Strains." Applied Sciences 8, no. 10: 1855.
Peculiarities of metals uptake by the biofuel crop Miscanthus x giganteus were explored during plant growth at soil from the military site (Sliač, Slovakia). The experiment was carried out in greenhouse during two vegetation seasons. Research soil was predominantly elevated in Fe and Ti, while other metals (As, Cu, Mn, Sr, Zn and Zr) were presented at order of magnitude lower concentrations. No inhibition of plant growth was observed. The calculated Bioconcentration Factor showed that levels of metals’ accumulation by plant roots, stems and leaves were independent of metals’ concentrations in the soil. The accumulation of metals by stems and leaves was much lower than by roots. As, Zr, Ti were almost not accumulated by stems and leaves during both seasons; accumulation of Cu, Fe, Mn, Zn and Sr was not essential which confirmed that biomass of M. x giganteus might be processed for the energy purpose.
Valentina V. Pidlisnyuk; Larry E. Erickson; Josef Trögl; Pavlo Shapoval; Jan Popelka; Lawrence C. Davis; Tetyana R. Stefanovska; Ganga M. Hettiarachchi. Metals uptake behaviour in Miscanthus x giganteus plant during growth at the contaminated soil from the military site in Sliač, Slovakia. PJCT 2018, 20, 1 -7.
AMA StyleValentina V. Pidlisnyuk, Larry E. Erickson, Josef Trögl, Pavlo Shapoval, Jan Popelka, Lawrence C. Davis, Tetyana R. Stefanovska, Ganga M. Hettiarachchi. Metals uptake behaviour in Miscanthus x giganteus plant during growth at the contaminated soil from the military site in Sliač, Slovakia. PJCT. 2018; 20 (2):1-7.
Chicago/Turabian StyleValentina V. Pidlisnyuk; Larry E. Erickson; Josef Trögl; Pavlo Shapoval; Jan Popelka; Lawrence C. Davis; Tetyana R. Stefanovska; Ganga M. Hettiarachchi. 2018. "Metals uptake behaviour in Miscanthus x giganteus plant during growth at the contaminated soil from the military site in Sliač, Slovakia." PJCT 20, no. 2: 1-7.
In the Protected Landscape Area “Labské pískovce” (Czech Republic), there are many sandstone caves accessible by permission only and where climbing equipment is commonly required. The tradition of visiting the caves dates back to turn of the 19th and 20th centuries, and visitors traditionally record their visits in log-books. We have gathered attendance data from 12 caves from log-books and via survey (2-year form collection period). The attendance varied from less than 10 to ~650 visitors annually. Signs of inadequate behavior of visitors were observed, especially waste disposal and smoking in the caves. Sediment step-compression increased significantly the ratios of fungi/bacteria and gram-positive to gram-negative (G+/G−) phospholipid fatty acids (PLFA) as well as PLFA of actinobacteria and activities of sediment phosphatases, glucosidases, and peroxidases. The number of visitors was correlated positively with the G+/G− ratio and sediment nutrients but negatively with sediment moisture and the activity of peroxidases. The results indicate a significant effect of attendance on sediment microbial communities, most likely caused by the import of nutrients into caves. Hence, reconsideration of the cave attendance policy is recommended.
Jaroslav Kukla; Michal Holec; Josef Trögl; Diana Holcová; Dagmar Hofmanová; Pavel Kuráň; Jan Popelka; Jan Pacina; Sylvie Kříženecká; Sergej Usťak; Roman Honzík. Tourist Traffic Significantly Affects Microbial Communities of Sandstone Cave Sediments in the Protected Landscape Area “Labské Pískovce” (Czech Republic): Implications for Regulatory Measures. Sustainability 2018, 10, 396 .
AMA StyleJaroslav Kukla, Michal Holec, Josef Trögl, Diana Holcová, Dagmar Hofmanová, Pavel Kuráň, Jan Popelka, Jan Pacina, Sylvie Kříženecká, Sergej Usťak, Roman Honzík. Tourist Traffic Significantly Affects Microbial Communities of Sandstone Cave Sediments in the Protected Landscape Area “Labské Pískovce” (Czech Republic): Implications for Regulatory Measures. Sustainability. 2018; 10 (2):396.
Chicago/Turabian StyleJaroslav Kukla; Michal Holec; Josef Trögl; Diana Holcová; Dagmar Hofmanová; Pavel Kuráň; Jan Popelka; Jan Pacina; Sylvie Kříženecká; Sergej Usťak; Roman Honzík. 2018. "Tourist Traffic Significantly Affects Microbial Communities of Sandstone Cave Sediments in the Protected Landscape Area “Labské Pískovce” (Czech Republic): Implications for Regulatory Measures." Sustainability 10, no. 2: 396.
Activated sludge biocenoses were compared on waste-water treatment plants in the city of Kazan, Russian Federation and the city of Teplice, Czech Republic. Based on Palia-Kovnatski index, Acanthamoeba in Kazan, Epistylis in Teplice, and Acanthamoeba and Centropyxis were dominant genera in both plants. The major subdominant generas identified were Arcella, Opercularia and Aspidisca. This indicates high nitrification ability, high water purification potential and matured activated sludge. Chemical composition of the waste-water was identified as the main factor determining the sludge biocenoses diversity. Higher sludge biodiversity (Shannon, Margalef, and Sorensen indexes) was found in Kazan corresponding to more concentrated inflow water.
Farida Y. Achmadulina; Рустем Закиров; Elena S. Balymova; Vera Denisova; Taťjána Brovdyová; Josef Trögl; Martin Neruda. Comparison of bioindicator eukaryotes of activated sludge biocenoses on two water-treatment plants: a case study. Nova Biotechnologica et chimica 2017, 16, 54 -60.
AMA StyleFarida Y. Achmadulina, Рустем Закиров, Elena S. Balymova, Vera Denisova, Taťjána Brovdyová, Josef Trögl, Martin Neruda. Comparison of bioindicator eukaryotes of activated sludge biocenoses on two water-treatment plants: a case study. Nova Biotechnologica et chimica. 2017; 16 (1):54-60.
Chicago/Turabian StyleFarida Y. Achmadulina; Рустем Закиров; Elena S. Balymova; Vera Denisova; Taťjána Brovdyová; Josef Trögl; Martin Neruda. 2017. "Comparison of bioindicator eukaryotes of activated sludge biocenoses on two water-treatment plants: a case study." Nova Biotechnologica et chimica 16, no. 1: 54-60.
Eva Kakosová; Pavel Hrabák; Miroslav Černík; Vít Novotný; Marie Czinnerová; Josef Trögl; Jan Popelka; Pavel Kuráň; Lenka Zoubková; Ľuboš Vrtoch. Effect of various chemical oxidation agents on soil microbial communities. Chemical Engineering Journal 2017, 314, 257 -265.
AMA StyleEva Kakosová, Pavel Hrabák, Miroslav Černík, Vít Novotný, Marie Czinnerová, Josef Trögl, Jan Popelka, Pavel Kuráň, Lenka Zoubková, Ľuboš Vrtoch. Effect of various chemical oxidation agents on soil microbial communities. Chemical Engineering Journal. 2017; 314 ():257-265.
Chicago/Turabian StyleEva Kakosová; Pavel Hrabák; Miroslav Černík; Vít Novotný; Marie Czinnerová; Josef Trögl; Jan Popelka; Pavel Kuráň; Lenka Zoubková; Ľuboš Vrtoch. 2017. "Effect of various chemical oxidation agents on soil microbial communities." Chemical Engineering Journal 314, no. : 257-265.
Living cells of the lux-based bioluminescent bioreporter Pseudomonas putida TVA8 were encapsulated in a silica hydrogel attached to the distal wider end of a tapered quartz fiber. Bioluminescence of immobilized cells was induced with toluene at high (26.5 mg/L) and low (5.3 mg/L) concentrations. Initial bioluminescence maxima were achieved after >12 h. One week after immobilization, a biofilm-like layer of cells had formed on the surface of the silica gel. This resulted in shorter response times and more intensive bioluminescence maxima that appeared as rapidly as 2 h after toluene induction. Considerable second bioluminescence maxima were observed after inductions with 26.5 mg toluene/L. The second and third week after immobilization the biosensor repetitively and semiquantitatively detected toluene in buffered medium. Due to silica gel dissolution and biofilm detachment, the bioluminescent signal was decreasing 20–32 days after immobilization and completely extinguished after 32 days. The reproducible formation of a surface cell layer on the wider end of the tapered optical fiber can be translated to various whole cell bioluminescent biosensor devices and may serve as a platform for in-situ sensors.
Gabriela Kuncová; Takayuki Ishizaki; Andrey Solovyev; Josef Trögl; Steven Ripp. The Repetitive Detection of Toluene with Bioluminescence Bioreporter Pseudomonas putida TVA8 Encapsulated in Silica Hydrogel on an Optical Fiber. Materials 2016, 9, 467 .
AMA StyleGabriela Kuncová, Takayuki Ishizaki, Andrey Solovyev, Josef Trögl, Steven Ripp. The Repetitive Detection of Toluene with Bioluminescence Bioreporter Pseudomonas putida TVA8 Encapsulated in Silica Hydrogel on an Optical Fiber. Materials. 2016; 9 (6):467.
Chicago/Turabian StyleGabriela Kuncová; Takayuki Ishizaki; Andrey Solovyev; Josef Trögl; Steven Ripp. 2016. "The Repetitive Detection of Toluene with Bioluminescence Bioreporter Pseudomonas putida TVA8 Encapsulated in Silica Hydrogel on an Optical Fiber." Materials 9, no. 6: 467.