Silicon (Si) enhances maize resistance to drought. While previous studies have mainly focused on the seedling stage, the mediation of drought stress by Si imposed at the vegetative and reproductive stages has been rarely investigated. A soil-column experiment was thus conducted under a rainproof shelter to quantify the effect s of Si application on the physiological and agronomic responses of maize to drought stress imposed at the 6-leaf (D-V6), 12-leaf (D-V12), and blister (D-R2) stages. The observed parameters included plant growth, photosynthesis, osmolytes, antioxidant activity, and grain yield. The results showed that drought stress strongly decreased the leaf area, leaf water content, photosynthetic rate, chlorophyll content, and antioxidant activity (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)) and markedly increased lipid peroxidation. D-V6, D-V12, and D-R2 decreased grain yields by 12.9%, 28.9%, and 44.8%, respectively, compared to the well-watered treatment (CK). However, Si application markedly increased leaf area, chlorophyll content, photosynthetic rate, osmolyte content, and enzymatic antioxidant activities (SOD, POD, and CAT), and decreased malondialdehyde (MDA) and superoxide radical accumulation, ultimately improving maize yields by 12.4%, 69.8%, and 80.8%, respectively, compared to the non-Si treated plants under drought stress at the V6, V12, and R2 stages. Furthermore, maize yields had a significant positive correlation with chlorophyll content and SOD and POD activity during the three stages. Our findings suggest that Si-induced changes in chlorophyll content and antioxidant activity might constitute important mechanisms for mitigating drought stress. In conclusion, this study provides physico-biochemical evidence for the beneficial role of Si in alleviating drought-induced yield reduction in maize, particularly during the late vegetative or early reproductive stages. Thus, Si application constitutes an effective approach for improving maize yield in rain-fed agricultural systems.

Introduction: Extreme weather has occurred more frequently in recent decades, which results in more frequent drought disasters in the maize growing season. Severe drought often decreases remarkably plant growth and yield of maize, and even reduces significantly the quality of maize production, especially for waxy maize.Results: To study the changes in plant growth, fresh ear yield, and fresh grain quality of waxy maize under water deficits occurring at different growth stages, and further strengthen the field water management of waxy maize, water deficit experiments were carried out under a rain shelter in 2019 and 2020. Water deficit treatments were imposed respectively at the V6–VT (D_V6–VT), VT–R2 (D_VT–R2), and R2–R3 (D_R2–R3) stages of waxy maize, and treatment with non-water deficit in the whole growing season was taken as the control (CK). The lower limit of soil water content was 50% of field capacity for a water deficit period and 65% of field capacity for a non-water deficit period.Results: In this study, water deficits imposed at V6–VT and VT–R2 stages decreased plant growth rate and leaf gas exchange parameters, accelerated leaf senescence, and limited ear growth of waxy maize, which resulted in 11.6% and 23.1% decreases in grains per ear, 19.4% and 7.3% declines in 100-grain weight, 20.3% and 14.2% losses in fresh ear yield in 2019 and 2020 growing seasons, respectively, while water deficit at R2–R3 stage had no significant effect on ear traits and fresh ear yield, but the fresh ear yield with husk of DR2–R3 decreased by 9.1% (P<0.05). The obvious water deficit imposed at the V6–VT and VT–R2 stages also lowered grain quality. Water deficits at the V6–VT and VT–R2 stages led to accelerated maturity, resulting in increased total protein, starch, and lysine content in grains at the R3 stage and decreased soluble sugar content. Principal component analysis revealed that when water deficits occurred in the waxy maize growing season, they firstly altered maize physiological processes, then affected ear characteristics and yield, and finally resulted in significant grain quality changes. In conclusion, a water deficit during V6–VT and VT–R2 not only reduced fresh ear yield but also adversely affected grain quality. However, water deficit during R2–R3 had little effect on total protein, starch, and soluble sugar content,but increased obviously lysine content.Discussion: The above results suggested that avoiding serious water deficits at the V6–VT and VT–R2 stages of waxy maize while imposing a slight water deficit at the R2–R3 stage has not only little effects on fresh ear yield but also a remarkable improvement in grain quality.

Soil water content (SWC, % vol) is a key factor affecting plant growth and development. SWC measurement is vital to rational use of water resources for irrigation, and the accuracy of sensors in SWC measurement is of significant importance to smart data‐driven irrigation. Here, a laboratory experiment and a field lysimetric experiment were conducted to evaluate the accuracy of Insentek sensors under various soil conditions (1.1 to 1.5 bulk densities and sand to clay soil textures) and irrigation levels (30, 45, and 60 mm), in 2018 and 2019. A microweighing lysimeter and oven‐drying method were used as standard methods to compare the Insentek method. The root mean square error (RMSE, % vol) and relative prediction deviation (RPD) between the Insentek and microlysimetric SWC values were 0.89–1.04% vol and 5.6–6.8, respectively, under laboratory condition. The RPD value is larger than the threshold value of 4.0, indicating the accuracy of the Insentek sensors is reliable under laboratory condition. Except for 60 mm irrigation treatment, the RMSE between Insentek and the oven‐drying method under field condition was 1.44–1.93% vol, and the RPD value was 1.56–1.93, lower than the threshold value of 4.0. The tiny gap between the Insentek sensor and soil may accelerate water infiltration along the probe 0‐3 d after irrigation while increase air filling 5–7 d after irrigation, causing greater RMSE and lower RPD values. The dissatisfied performance in field condition may also be associated with the obvious drawbacks of oven‐drying method, such as disturbance in soil sampling. When using oven‐drying method to analyze the accuracy of the Insentek sensors in field condition, the concerns should be well addressed.

The temperature-based crop water stress index (CWSI) can accurately reflect the extent of crop water deficit. As an ideal carrier of onboard thermometers to monitor canopy temperature (T_c), center pivot irrigation systems (CPIS) have been widely used in precision irrigation. However, the determination of reliable CWSI thresholds for initiating the CPIS is still a challenge for a winter wheat–summer maize cropping system in the North China Plain (NCP). To address this problem, field experiments were carried out to investigate the effects of CWSI thresholds on grain yield (GY) and water use efficiency (WUE) of winter wheat and summer maize in the NCP. The results show that positive linear functions were fitted to the relationships between CWSI and canopy minus air temperature (T_c − T_a) (r² > 0.695), and between crop evapotranspiration (ET_c) and T_c (r² > 0.548) for both crops. To make analysis comparable, GY and WUE data were normalized to a range of 0.0 to 1.0, corresponding the range of CWSI. With the increase in CWSI, a positive linear relationship was observed for WUE (r² = 0.873), while a significant inverse relationship was found for the GY (r² = 0.915) of winter wheat. Quadratic functions were fitted for both the GY (r² = 0.856) and WUE (r² = 0.629) of summer maize. By solving the cross values of the two GY and WUE functions for each crop, CWSI thresholds were proposed as being 0.322 for winter wheat, and 0.299 for summer maize, corresponding to a T_c − T_a threshold value of 0.925 and 0.498 °C, respectively. We conclude that farmers can achieve the dual goals of high GY and high WUE using the optimal thresholds proposed for a winter wheat–summer maize cropping system in the NCP.

A sustainable management strategy of soil fertility and cropping system is critical to guaranteeing food security. However, little is known about the effects of soil amendment strategies on crop growth via regulating soil moisture and photosynthesis in a ridge and furrow cropping system. Here, field experiments were carried out in 2017 and 2018 in semi-arid areas of Loess Plateau, northwest China to investigate the effects of integrated use of ridge and furrow planting and manure amendment on grain yields of maize. Four treatments were designed: CK (flat planting with 100% chemical fertilizer), RFC (ridge and furrow planting with 100% chemical fertilizer), RFR (ridge and furrow planting with 100% control-released fertilizer), and RFM (ridge and furrow planting with 50% manure fertilizer + 50% N fertilizer). On average, RFM increased photosynthetic rates (Pn) by 74%, followed by RFR by 47%, and RFC by 26%, compared to CK. Also, stomatal conductance (Cd), transpiration rates (Tr), and intercellular CO₂ concentration (Ci) were highest with RFM, followed by RFR and RFC. Averaged across the two years, RFM conserved 10% more soil water storage (SWS) than CK did at harvest, followed by RFR with an increment by 8%. However, RFC consumed more soil water than CK did, with its ET_c 8% higher than CK. Consequently, spring maize treated with RFM suffered less drought stress, especially in 2017 when precipitation was insufficient. On average, grain yields and water use efficiency of RFM were increased by 18% and 27%, compared to CK. Structural equation modeling analysis showed that there existed significant positive correlation between SWS in top layers and grain yields, while SWS in deep layers had negative effects on grain yields. In conclusion, the incorporation of manure into ridge and furrow planting system can be an efficient agronomic practice to improve plant photosynthesis, optimize soil moisture, and boost grain yields in semi-arid areas of Loess Plateau, northwest China.