Summary: Changes in the performance of genotypes in different environments are defined as genotype × environment (G×E) interactions. In grapevine (Vitis vinifera), complex interactions between different genotypes and climate, soil and farming practices yield unique berry qualities. However, the molecular basis of this phenomenon remains unclear. To dissect the basis of grapevine G×E interactions we characterized berry transcriptome plasticity, the genome methylation landscape and within‐genotype allelic diversity in two genotypes cultivated in three different environments over two vintages. We identified, through a novel data‐mining pipeline, genes with expression profiles that were: unaffected by genotype or environment, genotype‐dependent but unaffected by the environment, environmentally‐dependent regardless of genotype, and G×E‐related. The G×E‐related genes showed different degrees of within‐cultivar allelic diversity in the two genotypes and were enriched for stress responses, signal transduction and secondary metabolism categories. Our study unraveled the mutual relationships between genotypic and environmental variables during G×E interaction in a woody perennial species, providing a reference model to explore how cultivated fruit crops respond to diverse environments. Also, the pivotal role of vineyard location in determining the performance of different varieties, by enhancing berry quality traits, was unraveled.

Magna Graecia is the ancient name for the modern geopolitical region of South Italy extensively populated by Greek colonizers, shown by archeological and historical evidence to be the oldest wine growing region of Italy, crucial for the spread of specialized viticulture around Mediterranean shores. Here, the genetic diversity of Magna Graecia grape germplasm was assessed and its role in grapevine propagation around the Mediterranean basin was underlined. A large collection of grapevines from Magna Graecia was compared with germplasm from Georgia to the Iberian Peninsula using the 18 K SNP array. A high level of genetic diversity of the analyzed germplasm was determined; clustering, structure analysis and DAPC (Discriminant Analysis of Principal Components) highlighted the genetic relationships among genotypes from South Italy and the Eastern Mediterranean (Greece). Gene flow from east (Georgia) to west (Iberian Peninsula) was identified throughout the large number of detected admixed samples. Pedigree analysis showed a complex and well-structured network of first degree relationships, where the cultivars from Magna Graecia were mainly involved. This study provided evidence that Magna Graecia germplasm was shaped by historical events that occurred in the area due to the robust link between South Italian and Greek genotypes, as well as, by the availability of different thermal resources for cultivars growing in such different winegrowing areas. The uniqueness of this ampelographic platform was mainly an outcome of complex natural or human-driven crosses involving elite cultivars.

Although many studies in the literature illustrate the numerous devices and methodologies nowadays existing for assessing the spatial variability within agricultural fields, and indicate the potential for variable-rate irrigation (VRI) in vineyards, only very few works deal with the implementation of VRI systems to manage such heterogeneity, and these studies are usually conducted in experimental fields for research aims. In this study, a VR drip irrigation system was designed for a 1-ha productive vineyard in Northern Italy and managed during the agricultural season 2018, to demonstrate feasibility and effectiveness of a water supply differentiated according to the spatial variability detected in field. Electrical resistivity maps obtained by means of an electro-magnetic induction sensor were used to detect four homogeneous zones with similar soil properties. In each zone, a soil profile was opened, and soil samples were taken and analyzed in laboratory. Two irrigation management zones (MZs) were identified by grouping homogeneous zones on the basis of their hydrological properties, and an irrigation prescription map was built consistently with the total available water (TAW) content in the root zone of the two MZs. The designed drip irrigation system consisted of three independent sectors: the first two supplied water to the two MZs, while the third sector (reference sector) was managed following the farmer’s habits. During the season, irrigation in the first two sectors was fine-tuned using information provided by soil moisture probes installed in each sector. Results showed a reduction of water use by 18% compared to the ‘reference’ sector without losses in yield and product quality, and a grape’s maturation more homogeneous in time.

Estimating leaf area index (LAI) of Vitis vinifera using indirect methods involves some critical issues, related to its discontinuous and non-homogeneous canopy. This study evaluates the smart app PocketLAI and hemispherical photography in vineyards against destructive LAI measurements. Data were collected during six surveys in an experimental site characterized by a high level of heterogeneity among plants, allowing us to explore a wide range of LAI values. During the last survey, the possibility to combine remote sensing data and in-situ PocketLAI estimates (smart scouting) was evaluated. Results showed a good agreement between PocketLAI data and direct measurements, especially for LAI ranging from 0.13 to 1.41 (R² = 0.94, RRMSE = 17.27%), whereas the accuracy decreased when an outlying value (vineyard LAI = 2.84) was included (R² = 0.77, RRMSE = 43.00%), due to the saturation effect in case of very dense canopies arising from lack of green pruning. The hemispherical photography showed very high values of R², even in presence of the outlying value (R² = 0.94), although it showed a marked and quite constant overestimation error (RRMSE = 99.46%), suggesting the need to introduce a correction factor specific for vineyards. During the smart scouting, PocketLAI showed its reliability to monitor the spatial-temporal variability of vine vigor in cordon-trained systems, and showed a potential for a wide range of applications, also in combination with remote sensing.