Although many studies in the literature illustrate the numerous devices and methodologies nowadays existing for assessing the spatial variability within agricultural fields, and indicate the potential for variable-rate irrigation (VRI) in vineyards, only very few works deal with the implementation of VRI systems to manage such heterogeneity, and these studies are usually conducted in experimental fields for research aims. In this study, a VR drip irrigation system was designed for a 1-ha productive vineyard in Northern Italy and managed during the agricultural season 2018, to demonstrate feasibility and effectiveness of a water supply differentiated according to the spatial variability detected in field. Electrical resistivity maps obtained by means of an electro-magnetic induction sensor were used to detect four homogeneous zones with similar soil properties. In each zone, a soil profile was opened, and soil samples were taken and analyzed in laboratory. Two irrigation management zones (MZs) were identified by grouping homogeneous zones on the basis of their hydrological properties, and an irrigation prescription map was built consistently with the total available water (TAW) content in the root zone of the two MZs. The designed drip irrigation system consisted of three independent sectors: the first two supplied water to the two MZs, while the third sector (reference sector) was managed following the farmer’s habits. During the season, irrigation in the first two sectors was fine-tuned using information provided by soil moisture probes installed in each sector. Results showed a reduction of water use by 18% compared to the ‘reference’ sector without losses in yield and product quality, and a grape’s maturation more homogeneous in time.

Single nucleotide polymorphism (SNP) genotyping arrays are powerful tools to measure the level of genetic polymorphism within a population. The coming of next-generation sequencing technologies led to identifying thousands and millions of SNP loci useful in assessing the genetic diversity. The Vitis genotyping array, containing 18k SNP loci, has been developed and used to detect genetic diversity of Vitis vinifera germplasm. So far, this array was not validated on non-vinifera genotypes used as grapevine rootstocks. In this work, a core collection of 70 grapevine rootstocks, composed of individuals belonging to Vitis species not commonly used in the breeding programs, was genotyped using the 18k SNP genotyping array. SNP results were compared to the established SSR (Simple Sequence Repeat) markers in terms of heterozygosity and genetic structure of the core collection. Genotyping array has proved to be a valuable tool for genotyping of grapevine rootstocks, with more than 90% of SNPs successfully amplified. Structure analysis detected a high degree of admixed genotypes, supported by the complex genetic background of non-vinifera germplasm. Moreover, SNPs clearly differentiated non-vinifera and vinifera germplasm. These results represent a first step in studying the genetic diversity of non-conventional breeding material that will be used to select rootstocks with high tolerance to limiting environmental conditions.

Most of the vineyards around the world are in areas characterized by seasonal drought, where water deficits and high temperatures represent severe constraints on the regular grapevine growth cycle. Although grapevines are well adapted to arid and semi-arid environments, water stress can cause physiological changes, from mild to irreversible. Screening of available Vitis spp. genetic diversity for new rootstock breeding programs has been proposed as a way for which new viticulture challenges may be faced. In 2014, novel genotypes (M-rootstocks) were released from the University of Milan. In this work, the behavior of M1, M3 and M4 in response to decreasing water availabilities (80%, 50% and 20% soil water content, SWC) was investigated at the physiological and gene expression levels, evaluating gas exchange, stem water potential and transcript abundances of key genes related to ABA (abscisic acid) biosynthesis (VvZEP, VvNCED1 and VvNCED2) and signaling (VvPP2C4, VvSnRK2.6 and VvABF2), and comparing them to those of cuttings of nine commercial rootstocks widely used in viticulture. M-rootstocks showed a change at physiological levels in severe water-stressed conditions (20% soil water content, SWC), reducing the stomatal conductance and stem water potential, but maintaining high photosynthetic activity. Water use efficiency was high in water-limiting conditions. The transcriptional changes were observed at 50% SWC, with an increment of transcripts of VvNCED1 and VvNCED2 genes. M-rootstocks showed similar behavior to 1103P and 110R rootstocks, two highly tolerant commercial genotypes. These rootstocks adopted a tolerant strategy to face water-stressed conditions.

Grapevines worldwide are grafted onto Vitis spp. rootstocks in order to improve their tolerance to biotic and abiotic stresses. Thus, the response of vines to drought is the result of the interaction between the scion variety and the rootstock genotype. In this work, the responses of genotypes to drought were evaluated on 1103P and 101-14MGt plants, own-rooted and grafted with Cabernet Sauvignon, in three different water deficit conditions (80, 50, and 20% soil water content, SWC). Gas exchange parameters, stem water potential, root and leaf ABA content, and root and leaf transcriptomic response were investigated. Under well-watered conditions, gas exchange and stem water potential were mainly affected by the grafting condition, whereas under sever water deficit they were affected by the rootstock genotype. Under severe stress conditions (20% SWC), 1103P showed an “avoidance” behavior. It reduced stomatal conductance, inhibited photosynthesis, increased ABA content in the roots, and closed the stomata. The 101-14MGt maintained a high photosynthetic rate, limiting the reduction of soil water potential. This behavior results in a “tolerance” strategy. An analysis of the transcriptome showed that most of the differentially expressed genes were detected at 20% SWC, and more significantly in roots than in leaves. A core set of genes has been highlighted on the roots as being related to the root response to drought that are not affected by genotype nor grafting. Genes specifically regulated by grafting and genes specifically regulated by genotype under drought conditions have been identified as well. The 1103P, more than the 101-14MGt, regulated a high number of genes in both own-rooted and grafted conditions. This different regulation revealed that 1103P rootstock readily perceived the water scarcity and rapidly faced the stress, in agreement with its avoidance strategy.

In recent decades, worldwide wine-growing regions have been affected by increasingly more frequent effects of climate change, such as long period of droughts during the growing season, summer heat waves, and late spring frost events, thus causing concern for the grape quality and production. In this context, it is necessary to develop innovative agronomic practices to counter the various negative effects from those extreme weather events, by equipping the vineyards with effective and reliable multifunctional systems, which are also economically sustainable. Particularly, a multifunctional irrigation system can be used to reduce the risk of extreme weather events and, at the same time, to improve quality and quantity of grape production, reducing their inter-annual variability as well, by providing an optimized plant water nutrition. In a vineyard situated south of Lake Garda (Northern Italy), a multifunctional irrigation system equipped with drippers and mini-sprinklers (the latter to protect from both late spring frost and summer high-temperature event) was assessed. The results obtained for the growing season 2020–21 showed that the optimized drip irrigation reduced water consumption without affecting the grape yield, both in quantity and quality. The frost protection operated by mini-sprinklers increased the air temperature at bud’s level of about 1 °C, suggesting a positive effect on plant production. Finally, in 2020, must quality was positively affected by summer sprinkler irrigation, increasing the levels of malic acid and titratable acidity of over 0.7 g L⁻¹ while lowered total soluble solids. Further activities in the 2022 season intend to better assess the water use efficiency of this promising multifunctional system.

How accurately do Sentinel-2 (S2) images describe vine row spatial variability? Can they produce effective management zones (MZs) for precision viticulture? S2 and UAV datasets acquired over two years for different drip-irrigated vineyards in the Colli Morenici region (northern Italy) were used to assess the actual need to use UAV-NDVI maps instead of S2 images to obtain effective MZ maps. First, the correlation between S2 and UAV-NDVI values was investigated. Secondly, contingency matrices and dichotomous tables (considering UAV-MZ maps as a reference) were developed to compare MZ maps produced using S2 and UAV imagery. Moreover, data on grape production and quality were analyzed through linear discrimination analyses (LDA) to evaluate the effectiveness of S2-MZs and UAV-MZs to explain spatial variability in yield and quality data. The outcomes highlight that S2 images can be quite good tools to manage fertilization based on the within-field vigor variability, of which they capture the main features. Nevertheless, as S2-MZs with low and high vigor were over-estimated, S2-MZ maps cannot be used for high-accuracy input management. From the LDA results, the UAV-MZs appeared slightly more performant than the S2-MZs in explaining the variability in grape quality and yield, especially in the case of low-vigor MZs.