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Ángel Gil-Izquierdo
Department of Food Science and Technology, Research Group on Quality, Safety and Bioactivity of Plant Foods, CEBAS (CSIC), Campus Espinardo, 30100 Murcia, Spain

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Journal article
Published: 06 August 2021 in Microchemical Journal
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Presently, the attention given to natural substances to counteract damage produced by oxidative stress (OS) has risen sharply. In this scenario, hydroxytyrosol (HT) derivatives, formed as a result of HT conjugation with fatty acids (FAs) (lipophenols), have been recently described in foodstuffs such as extra virgin olive oil, as being powerful bioactive compounds with a higher activity than the unesterified phenolic compound. The present work describes the capacity of HT lipophenols to act on the course of OS and secondary inflammatory processes, based on their capacity to modulate the isoprostanoid profile induced by H2O2 in THP-1 monocytic cells. A UHPLC-QqQ-ESI-MS/MS-based lipidomics workflow was applied over a range of 37 human oxylipins. The main outcomes retrieved suggest both HT and HT-lipophenols as regulators of the cellular redox balance, acting as pro-oxidants in vitro, which is highly dependent on the experimental conditions. Our outcomes suggest the anti-inflammatory potential of both HT and HT-lipophenols, where the type of the FAs on the HT core appears to be critical for defining the bioactivity of lipophenols, highlighting that a lipidomic approach, with the simultaneous analysis of multiple oxylipins, is critical for the understanding of the bioactivity of lipophenols on isoprostanoid generation and hence, on pathophysiological processes.

ACS Style

Carolina Alemán-Jiménez; Raúl Domínguez-Perles; Federico Fanti; Juana I. Gallego-Gómez; Agustín Simonelli-Muñoz; Espérance Moine; Thierry Durand; Céline Crauste; Ángel Gil-Izquierdo; Sonia Medina. Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow. Microchemical Journal 2021, 170, 106703 .

AMA Style

Carolina Alemán-Jiménez, Raúl Domínguez-Perles, Federico Fanti, Juana I. Gallego-Gómez, Agustín Simonelli-Muñoz, Espérance Moine, Thierry Durand, Céline Crauste, Ángel Gil-Izquierdo, Sonia Medina. Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow. Microchemical Journal. 2021; 170 ():106703.

Chicago/Turabian Style

Carolina Alemán-Jiménez; Raúl Domínguez-Perles; Federico Fanti; Juana I. Gallego-Gómez; Agustín Simonelli-Muñoz; Espérance Moine; Thierry Durand; Céline Crauste; Ángel Gil-Izquierdo; Sonia Medina. 2021. "Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow." Microchemical Journal 170, no. : 106703.

Journal article
Published: 13 July 2021 in Nutrients
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The effect of coffee and cocoa on oxidative damage to macromolecules has been investigated in several studies, often with controversial results. This study aimed to investigate the effect of one-month consumption of different doses of coffee or cocoa-based products containing coffee on markers of DNA damage and lipid peroxidation in young healthy volunteers. Twenty-one volunteers were randomly assigned into a three-arm, crossover, randomized trial. Subjects were assigned to consume one of the three following treatments: one cup of espresso coffee/day (1C), three cups of espresso coffee/day (3C), and one cup of espresso coffee plus two cocoa-based products containing coffee (PC) twice per day for 1 month. At the end of each treatment, blood samples were collected for the analysis of endogenous and H2O2-induced DNA damage and DNA oxidation catabolites, while urines were used for the analysis of oxylipins. On the whole, four DNA catabolites (cyclic guanosine monophosphate (cGMP), 8-OH-2′-deoxy-guanosine, 8-OH-guanine, and 8-NO2-cGMP) were detected in plasma samples following the one-month intervention. No significant modulation of DNA and lipid damage markers was documented among groups, apart from an effect of time for DNA strand breaks and some markers of lipid peroxidation. In conclusion, the consumption of coffee and cocoa-based confectionery containing coffee was apparently not able to affect oxidative stress markers. More studies are encouraged to better explain the findings obtained and to understand the impact of different dosages of these products on specific target groups.

ACS Style

Daniela Martini; Raúl Domínguez-Perles; Alice Rosi; Michele Tassotti; Donato Angelino; Sonia Medina; Cristian Ricci; Alexandre Guy; Camille Oger; Letizia Gigliotti; Thierry Durand; Mirko Marino; Hans Gottfried-Genieser; Marisa Porrini; Monica Antonini; Alessandra Dei Cas; Riccardo Bonadonna; Federico Ferreres; Francesca Scazzina; Furio Brighenti; Patrizia Riso; Cristian Del Bo’; Pedro Mena; Angel Gil-Izquierdo; Daniele Del Rio. Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study. Nutrients 2021, 13, 2399 .

AMA Style

Daniela Martini, Raúl Domínguez-Perles, Alice Rosi, Michele Tassotti, Donato Angelino, Sonia Medina, Cristian Ricci, Alexandre Guy, Camille Oger, Letizia Gigliotti, Thierry Durand, Mirko Marino, Hans Gottfried-Genieser, Marisa Porrini, Monica Antonini, Alessandra Dei Cas, Riccardo Bonadonna, Federico Ferreres, Francesca Scazzina, Furio Brighenti, Patrizia Riso, Cristian Del Bo’, Pedro Mena, Angel Gil-Izquierdo, Daniele Del Rio. Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study. Nutrients. 2021; 13 (7):2399.

Chicago/Turabian Style

Daniela Martini; Raúl Domínguez-Perles; Alice Rosi; Michele Tassotti; Donato Angelino; Sonia Medina; Cristian Ricci; Alexandre Guy; Camille Oger; Letizia Gigliotti; Thierry Durand; Mirko Marino; Hans Gottfried-Genieser; Marisa Porrini; Monica Antonini; Alessandra Dei Cas; Riccardo Bonadonna; Federico Ferreres; Francesca Scazzina; Furio Brighenti; Patrizia Riso; Cristian Del Bo’; Pedro Mena; Angel Gil-Izquierdo; Daniele Del Rio. 2021. "Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study." Nutrients 13, no. 7: 2399.

Research article
Published: 01 June 2021 in Journal of the Science of Food and Agriculture
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BACKGROUND Juices are currently the fast growing segment in the fruit and vegetable industry sector. However, there are still no reports on the diversity of phytochemical profile and health-promoting properties of commercial sea buckthorn (Hippophaë rhamnoides) juices. This study aimed to identify and quantify phytoprostanes, phytofurans (UHPLC-QqQ-MS/MS), tocopherols, tocotrienols (UPLC-FL), carotenoids and free amino acids (UPLC-PDA-Q/TOF-MS) and assess the anti-cholinergic, anti-diabetic, anti-obesity, anti-inflammatory and antioxidant potential by in vitro assays of commercial sea buckthorn juices. RESULTS PhytoPs and PhytoFs in sea buckthorn juices were identified for the first time. Juices contained eight F1-, D1-, B1- and L1-phytoprostanes and one phytofuran (32.31-1523.51 ng and up to 101.47 μg/100 g dry weight (DW)), four tocopherol congeners (22.23-94.08 mg/100 g DW) and three tocotrienols (5.93-25.34 mg/100 g DW). 18 carotenoids were identified, including 10 xanthophylls, 7 carotenes and phytofluene, at a concentration of 133.65 to 839.89 mg/100 g DW. Among the 20 amino acids (175.92-1,822.60 mg/100 g DW) asparagine was dominant, and essential and conditionally essential amino acids constituted 11 to 41% of the total. The anti-enzyme and antioxidant potential of juices correlated selectively with the composition. CONCLUSION Sea buckthorn juice can be a valuable dietary source of vitamins E and A, oxylipins and amino acids, used in the prevention of metabolic syndrome, inflammation and neurodegenerative processes. The differentiation of composition and bioactive potential of commercial juices indicate that for the consumer it should be important to choose juices from the declared berry cultivars and crops.

ACS Style

Karolina Tkacz; Ángel Gil‐Izquierdo; Sonia Medina; Igor Piotr Turkiewicz; Raúl Domínguez‐Perles; Paulina Nowicka; Aneta Wojdyło. Phytoprostanes, phytofurans, tocopherols, tocotrienols, carotenoids and free amino acids and biological potential of sea buckthorn juices. Journal of the Science of Food and Agriculture 2021, 1 .

AMA Style

Karolina Tkacz, Ángel Gil‐Izquierdo, Sonia Medina, Igor Piotr Turkiewicz, Raúl Domínguez‐Perles, Paulina Nowicka, Aneta Wojdyło. Phytoprostanes, phytofurans, tocopherols, tocotrienols, carotenoids and free amino acids and biological potential of sea buckthorn juices. Journal of the Science of Food and Agriculture. 2021; ():1.

Chicago/Turabian Style

Karolina Tkacz; Ángel Gil‐Izquierdo; Sonia Medina; Igor Piotr Turkiewicz; Raúl Domínguez‐Perles; Paulina Nowicka; Aneta Wojdyło. 2021. "Phytoprostanes, phytofurans, tocopherols, tocotrienols, carotenoids and free amino acids and biological potential of sea buckthorn juices." Journal of the Science of Food and Agriculture , no. : 1.

Journal article
Published: 06 April 2021 in Molecules
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Caffeine is a food supplement widely consumed by athletes, but it has not been established. So far, the veracity of their labeling in terms of the dosage and cause/effect relationship aimed at the consumer. The aim is to analyze the health claims and the dosage presented on the labeling of caffeine supplements and to evaluate if they follow the European Food Safety Authority (EFSA) and international criteria. A descriptive cross-sectional study of a sample of caffeine supplements was carried out. The search was done through the Amazon and Google Shopping web portals. In order to assess the adequacy of the health claims, the guidelines of reference established by European Food Safety Authority were compared to the Academy of Nutrition and Dietetics, International Olympic Committee, and Australian Institute of Sport guidelines; in addition, recent systematic reviews were addressed. A review of labels of 42 caffeine supplements showed that, in less than 3% of the products were the health claims supported by the recommendations and by the labeled quantity of caffeine. The claims that fully complied the recommendations were, “improves or increases endurance performance”, “improves strength performance”, or “improves short-term performance”. In most cases, the recommended dosage was 200 mg/day for these products, which is the minimum for the caffeine effects to be declared. The rest of the health claims were not adequate or need to be modified. Most of the health claims identified indicated an unproven cause and effect, which constitutes consumer fraud, and so must be modified or eliminated.

ACS Style

Pedro Estevan Navarro; Isabel Sospedra; Alejandro Perales; Cristina González-Díaz; Rubén Jiménez-Alfageme; Sonia Medina; Angel Gil-Izquierdo; José Martínez-Sanz. Caffeine Health Claims on Sports Supplement Labeling. Analytical Assessment According to EFSA Scientific Opinion and International Evidence and Criteria. Molecules 2021, 26, 2095 .

AMA Style

Pedro Estevan Navarro, Isabel Sospedra, Alejandro Perales, Cristina González-Díaz, Rubén Jiménez-Alfageme, Sonia Medina, Angel Gil-Izquierdo, José Martínez-Sanz. Caffeine Health Claims on Sports Supplement Labeling. Analytical Assessment According to EFSA Scientific Opinion and International Evidence and Criteria. Molecules. 2021; 26 (7):2095.

Chicago/Turabian Style

Pedro Estevan Navarro; Isabel Sospedra; Alejandro Perales; Cristina González-Díaz; Rubén Jiménez-Alfageme; Sonia Medina; Angel Gil-Izquierdo; José Martínez-Sanz. 2021. "Caffeine Health Claims on Sports Supplement Labeling. Analytical Assessment According to EFSA Scientific Opinion and International Evidence and Criteria." Molecules 26, no. 7: 2095.

Journal article
Published: 26 March 2021 in Agronomy
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Halophytes are capable of coping with excessive NaCl in their tissues, although some species may differ in their degree of salt tolerance. In addition, it is not clear whether they can tolerate other confounding factors and impurities associated with non-conventional waters. The experiment was performed in a greenhouse with Crithmum maritimum and Atriplex halimus plants, growing on soil and irrigated with two different water types: reclaimed wastewater (RWW) (EC: 0.8–1.2 dS m−1) and reverse osmosis brine (ROB) (EC: 4.7–7.9 dS m−1). Both species showed different physiological and nutritional responses, when they were irrigated with ROB. Atriplex plants reduced leaf water potential and maintained leaf turgor as consequence of an osmotic adjustment process. Atriplex showed higher intrinsic water use efficiency than Crithmum, regardless of the type of water used. In Crithmum, the water status and photosynthetic efficiency were similar in both treatments. Crithmum presented a higher leaf accumulation of B and Ca ions, while Atriplex a higher amount of K, Mg, Na and Zn. Crithmum plants irrigated with ROB presented higher concentrations of 1-aminocyclopropane-1-carboxylic acid and trans-zeatin-glucoside, whereas abscisic acid concentration was lower. Atriplex showed a lower concentration of trans-zeatin-riboside and scopoletin. The characteristics associated to water irrigation did not influence negatively the development of any of these species, which confirms the use of brine as an alternative to irrigate them with conventional waters.

ACS Style

María Gómez-Bellot; Beatriz Lorente; María Ortuño; Sonia Medina; Ángel Gil-Izquierdo; Sebastián Bañón; María Sánchez-Blanco. Recycled Wastewater and Reverse Osmosis Brine Use for Halophytes Irrigation: Differences in Physiological, Nutritional and Hormonal Responses of Crithmum maritimum and Atriplex halimus Plants. Agronomy 2021, 11, 627 .

AMA Style

María Gómez-Bellot, Beatriz Lorente, María Ortuño, Sonia Medina, Ángel Gil-Izquierdo, Sebastián Bañón, María Sánchez-Blanco. Recycled Wastewater and Reverse Osmosis Brine Use for Halophytes Irrigation: Differences in Physiological, Nutritional and Hormonal Responses of Crithmum maritimum and Atriplex halimus Plants. Agronomy. 2021; 11 (4):627.

Chicago/Turabian Style

María Gómez-Bellot; Beatriz Lorente; María Ortuño; Sonia Medina; Ángel Gil-Izquierdo; Sebastián Bañón; María Sánchez-Blanco. 2021. "Recycled Wastewater and Reverse Osmosis Brine Use for Halophytes Irrigation: Differences in Physiological, Nutritional and Hormonal Responses of Crithmum maritimum and Atriplex halimus Plants." Agronomy 11, no. 4: 627.

Journal article
Published: 17 March 2021 in Free Radical Biology and Medicine
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Inflammation is a fundamental pathophysiological process which occurs in the course of several diseases. The present work describes the capacity of phytoprostanes (PhytoPs) and phytofurans (PhytoFs) (plant oxylipins), present in plant-based foods, to modulate inflammatory processes mediated by prostaglandins (PGs, human oxylipins) in lipopolysaccharide (LPS)-stimulated THP-1 monocytic cells, through a panel of 21 PGs and PG's metabolites, analyzed by UHPLC-QqQ-ESI-MS/MS. Also, the assessment of the cytotoxicity of PhytoPs and PhytoFs on THP-1 cells evidenced percentages of cell viability higher than 90% when treated with up to 100 μM. Accordingly, 50 μM of the individual PhytoPs and PhytoFs 9-F1t-PhytoP, 9-epi-9-F1t-PhytoP, ent-16-F1t-PhytoP, ent-16-epi-16-F1t-PhytoP, ent-9-D1t-PhytoP, 16-B1-PhytoP, 9-L1-PhytoP, ent-16(RS)-9-epi-ST-Δ14-10-PhytoF, ent-9(RS)-12-epi-ST-Δ10-13-PhytoF, and ent-16(RS)-13-epi-ST-Δ14-9-PhytoF were evaluated on their capacity to modulate the expression of inflammatory markers. The results obtained demonstrated the presence of 7 metabolites (15-keto-PGF2α, PGF2α, 11β-PGF2α, PGE2, PGD2, PGDM, and PGF1α) in THP-1 monocytic cells, which expression was significantly modulated when exposed to LPS. The evaluation of the capacity of the individual PhytoPs and PhytoFs to revert the modification of the quantitative profile of PGs induced by LPS revealed the anti-inflammatory ability of 9-F1t-PhytoP, ent-9-D1t-PhytoP, 16-B1-PhytoP, 9-L1-PhytoP, and ent-9(RS)-12-epi-ST-Δ10-13-PhytoF, as evidenced by their capacity to prevent the up-regulation of 15-keto-PGF2α, PGF2α, PGE2, PGF1α, PGDM, and PGD2 induced by LPS. These results indicated that specific plant oxylipins can protect against inflammatory events, encouraging further investigations using plant-based foods rich in these oxylipins or enriched extracts, to identify specific bioactivities of the diverse individual molecules, which can be useful for nutrition and health in the frame of well-defined pathophysiological processes.

ACS Style

María Campillo; Sonia Medina; Federico Fanti; Juana I. Gallego-Gómez; Agustín Simonelli-Muñoz; Valérie Bultel-Poncé; Thierry Durand; Jean Marie Galano; Francisco A. Tomás-Barberán; Ángel Gil-Izquierdo; Raúl Domínguez-Perles. Phytoprostanes and phytofurans modulate COX-2-linked inflammation markers in LPS-stimulated THP-1 monocytes by lipidomics workflow. Free Radical Biology and Medicine 2021, 167, 335 -347.

AMA Style

María Campillo, Sonia Medina, Federico Fanti, Juana I. Gallego-Gómez, Agustín Simonelli-Muñoz, Valérie Bultel-Poncé, Thierry Durand, Jean Marie Galano, Francisco A. Tomás-Barberán, Ángel Gil-Izquierdo, Raúl Domínguez-Perles. Phytoprostanes and phytofurans modulate COX-2-linked inflammation markers in LPS-stimulated THP-1 monocytes by lipidomics workflow. Free Radical Biology and Medicine. 2021; 167 ():335-347.

Chicago/Turabian Style

María Campillo; Sonia Medina; Federico Fanti; Juana I. Gallego-Gómez; Agustín Simonelli-Muñoz; Valérie Bultel-Poncé; Thierry Durand; Jean Marie Galano; Francisco A. Tomás-Barberán; Ángel Gil-Izquierdo; Raúl Domínguez-Perles. 2021. "Phytoprostanes and phytofurans modulate COX-2-linked inflammation markers in LPS-stimulated THP-1 monocytes by lipidomics workflow." Free Radical Biology and Medicine 167, no. : 335-347.

Public health
Published: 04 February 2021 in Frontiers in Public Health
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The objective of this study is to analyze the physical activity levels among the ethnic groups in La Guajira, Colombia, according to the different ethnic groups and their sociodemographic factors. With regards to ethnic groups, two groups were studied, ethnic (Indigenous and Afro-Colombian) and non-ethnic (White or Mestizo). In order to obtain the relevant data a non-probability sample of 251 people of ≥18 years of age were asked to complete the short version of the International Physical Activity Questionnaire (IPAQ). In this analysis, physical activity (METs.min−1) levels were considered as the dependent variable, while the independent variables were linked to the sociodemographic factors: sex, age, social class, civil status, educational level, and municipality of residence. The individuals were then categorized based on their physical activity levels and their compliance with the World Health Organization's (WHO) physical activity recommendations. Next, using the sociodemographic variables, regression models were made to determine the likelihood of the participants meeting these physical activity targets; these models found that 78.1% of the studied participants met the targets. According to the results of the bivariate analysis, participants of ≥47 years of age, and those with only a primary education presented a lower probability of complying with the physical activity recommendations, while those who lived in large municipalities (Riohacha) displayed a larger probability of compliance [OR = 2.16; 95% CI: (1.18–3.96)]. Furthermore, the multivariate analysis showed that Indigenous and Afro-Colombian people in a low social class are more likely to comply with the physical activity recommendations [ORad = 4.88; 95% CI: (1.31–18.1)], while residing in a smaller municipality (Manaure) is associated with a lower probability of compliance [ORad = 0.39; 95% CI: (0.16–0.91)]. In addition, the educated participants were shown to be active and to comply with the physical activity recommendations is to a high degree, even when compared with national and global results. In the ethnic group however, low social class stood out as the sociodemographic factor most associated with high physical activity, while Afro-Colombians were shown to be more active than the Indigenous people. The results of this study offer evidence that can either form a base for future research.

ACS Style

Yaina Panciera-Di-Zoppola; Juan Niño-Restrepo; José Melo-Freile; Rocío Ortiz-Moncada. Levels of Physical Activity in the Adult Population of La Guajira, Colombia: A Focus on Ethnicity. Frontiers in Public Health 2021, 8, 1 .

AMA Style

Yaina Panciera-Di-Zoppola, Juan Niño-Restrepo, José Melo-Freile, Rocío Ortiz-Moncada. Levels of Physical Activity in the Adult Population of La Guajira, Colombia: A Focus on Ethnicity. Frontiers in Public Health. 2021; 8 ():1.

Chicago/Turabian Style

Yaina Panciera-Di-Zoppola; Juan Niño-Restrepo; José Melo-Freile; Rocío Ortiz-Moncada. 2021. "Levels of Physical Activity in the Adult Population of La Guajira, Colombia: A Focus on Ethnicity." Frontiers in Public Health 8, no. : 1.

Journal article
Published: 22 January 2021 in Nutrients
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The intake of sugar-sweetened beverages has been associated with an augmented prevalence of metabolic diseases, namely, obesity, type II diabetes, and metabolic syndrome. On the other hand, nowadays, it is broadly accepted that foods and beverages rich in (poly)phenols could contribute to reducing the incidence of these pathologies. In this sense, the objective of the work was to revalue second quality citrus fruits for the development of new beverages, rich in anthocyanins and flavanones (maqui berry and second qualities citrus-based), and evaluate the influence of alternative sweeteners (sucralose, sucrose, or stevia), regarding the bioaccessibility and bioavailability of these bioactive compounds in the frame of a chronic (longitudinal) intervention. To fulfill this objective, a longitudinal study of the urinary excretion of anthocyanins and flavanones, after 2-months of ingestion of the developed maqui-citrus beverage, by 138 volunteers (n = 46 per beverage) and the analysis of the resulting phenolic metabolites by ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC-ESI-QqQ-MS/MS) was carried out. As major results, the bioavailable metabolites of caffeic acid (CA), catechol (CAT), 3,4-di-hydroxyphenylacetic acid (DHPAA), eriodictyol (E), homoeriodictyol (HE), hippuric acid (HA), naringenin (N), trans-ferulic acid (TFA), 2,4,6-tri-hydroxybenzaldehyde (THBA), trans-isoferulic acid (TIFA), and vanillic acid (VA) were detected. Accordingly, significantly different bioavailability was dependent on the sweetener used, allowing proposing stevia and, to a lower extent, sucralose, as valuable alternatives to sucrose.

ACS Style

Vicente Agulló; Cristina García-Viguera; Raúl Domínguez-Perles. Beverages Based on Second Quality Citrus Fruits and Maqui Berry, a Source of Bioactive (Poly)phenols: Sorting Out Urine Metabolites upon a Longitudinal Study. Nutrients 2021, 13, 312 .

AMA Style

Vicente Agulló, Cristina García-Viguera, Raúl Domínguez-Perles. Beverages Based on Second Quality Citrus Fruits and Maqui Berry, a Source of Bioactive (Poly)phenols: Sorting Out Urine Metabolites upon a Longitudinal Study. Nutrients. 2021; 13 (2):312.

Chicago/Turabian Style

Vicente Agulló; Cristina García-Viguera; Raúl Domínguez-Perles. 2021. "Beverages Based on Second Quality Citrus Fruits and Maqui Berry, a Source of Bioactive (Poly)phenols: Sorting Out Urine Metabolites upon a Longitudinal Study." Nutrients 13, no. 2: 312.

Journal article
Published: 12 January 2021 in Food Research International
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While the fruits of Xylopia aethiopica (Dunal) A. Rich. are important in African countries as a local trade product, their composition remains scarcely investigated. Phenolic fingerprint is herein delivered through HPLC-DAD-ESI(Ion Trap)-MSn and UPLC-ESI-QTOF-MS2 analysis, six cinnamoylquinic acid derivatives and twenty-four flavonoid glycosides being determined, chrysoeriol-7-O-glycosides being the main constituents. A cytotoxicity screening of twenty-eight hydroethanol extracts, obtained from a collection of Guinea-Bissauan plants, against A549 and AGS carcinoma cells, revealed the selective and potent effect towards AGS cells (IC50 = 151 × 10−3 g L−1), upon exposure to the extract from X. aethiopica fruits. Additional experiments demonstrated insignificant effect on LDH release at 151 × 10−3 g L−1, morphological analysis further suggesting induction of apoptosis. Pro-apoptotic effects were confirmed, as the extract enabled the activation of the effector caspase-3, broadening the knowledge on the anticancer mechanisms elicited by the fruits of X. aethiopica. Phenolic constituents might contribute to the cytotoxic effects, particularly via caspase-3 activation. Considering that X. aethiopica fruit is very often referred as an anticancer ingredient in Africa, but mainly the potent cytotoxicity herein recorded, our results call for additional research aiming to identify non-phenolic constituents contributing to the effects and also to further detail the anticancer mechanisms.

ACS Style

Vera Ribeiro; Federico Ferreres; Tiago Macedo; Ángel Gil-Izquierdo; Andreia P. Oliveira; Nelson G.M. Gomes; Luísa Araújo; David M. Pereira; Paula B. Andrade; Patrícia Valentão. Activation of caspase-3 in gastric adenocarcinoma AGS cells by Xylopia aethiopica (Dunal) A. Rich. fruit and characterization of its phenolic fingerprint by HPLC-DAD-ESI(Ion Trap)-MSn and UPLC-ESI-QTOF-MS2. Food Research International 2021, 141, 110121 .

AMA Style

Vera Ribeiro, Federico Ferreres, Tiago Macedo, Ángel Gil-Izquierdo, Andreia P. Oliveira, Nelson G.M. Gomes, Luísa Araújo, David M. Pereira, Paula B. Andrade, Patrícia Valentão. Activation of caspase-3 in gastric adenocarcinoma AGS cells by Xylopia aethiopica (Dunal) A. Rich. fruit and characterization of its phenolic fingerprint by HPLC-DAD-ESI(Ion Trap)-MSn and UPLC-ESI-QTOF-MS2. Food Research International. 2021; 141 ():110121.

Chicago/Turabian Style

Vera Ribeiro; Federico Ferreres; Tiago Macedo; Ángel Gil-Izquierdo; Andreia P. Oliveira; Nelson G.M. Gomes; Luísa Araújo; David M. Pereira; Paula B. Andrade; Patrícia Valentão. 2021. "Activation of caspase-3 in gastric adenocarcinoma AGS cells by Xylopia aethiopica (Dunal) A. Rich. fruit and characterization of its phenolic fingerprint by HPLC-DAD-ESI(Ion Trap)-MSn and UPLC-ESI-QTOF-MS2." Food Research International 141, no. : 110121.

Journal article
Published: 24 December 2020 in Journal of Ethnopharmacology
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According to ethnobotanical surveys, Cassia sieberiana DC. (1825) is a particularly reputed species in African folk Medicine, namely due to the application of its leaves and roots for the treatment of diseases and symptomatology that appear to be related with an inflammatory background. In contrast with the roots of the plant, the leaves remain to be investigated, which prompted us to further detail mechanisms underlying their anti-inflammatory properties, by using in vitro models of disease. Considering its use in the amelioration and treatment of conditions that frequently underlie an inflammatory response, C. sieberiana leaves extract was prioritized amongst a collection of extracts obtained from plants collected in Guinea-Bissau. As such, this work aims to deliver experimental data on the anti-inflammatory properties of C. sieberiana leaf and to establish possible associations with its chemical composition, thus providing a rationale on its use in folk Medicine. The chemical profile of an hydroethanol extract obtained from the leaves of the plant was established by HPLC-DAD-ESI/MSn in order to identify bioactives. The extract and its main compound were tested towards a series of inflammatory mediators, both in enzymatic and cell-based models. The capacity to interfere with the eicosanoid-metabolizing enzymes 5-lipoxygenase (5-LOX), cyclooxygenase-1 (COX-1) and -2 (COX-2) was evaluated in cell-free systems, while the effects in interleukin 6 (IL-6) and tumour necrosis factor-α (TNF-α) levels produced by THP-1 derived macrophages were assessed through ELISA. HPLC-DAD-ESI/MSn analysis of the extract elucidated a chemical profile qualitatively characterized by a series of anthraquinones, particularly rhein derivatives, and nine flavonols, most of which 3-O-glycosylated. Considering the concentrations of the identified compounds, quercetin was detached as the main component. Effects of the hydroethanol extract obtained from C. sieberiana leaves against key enzymes of the arachidonic acid cascade were recorded, namely a concentration-dependent inhibition against 5-LOX, at concentrations ranging from 16 to 250 μg mL-1 and a selective inhibitory action upon COX-2 (IC50 = 3.58 μg mL-1) in comparison with the isoform COX-1 (IC50 = 9.10 μg mL-1). Impact on inflammatory cytokines was also noted, C. sieberiana leaf extract significantly decreasing IL-6 levels in THP-1 derived macrophages at 250 and 500 μg mL-1. In contrast, TNF-α levels were found to be increased in the same model. Quercetin appears to partially account for the observed effects, namely due to the significant inhibitory effects on the activity of the arachidonic acid metabolizing enzymes COX-2 and 5-LOX. The anti-inflammatory effects herein reported provide a rationale for the use of C. sieberiana leaves in African folk practices, such as in the treatment of arthritis, rheumatism and body aches. Considering the occurrence of flavonoidic and anthraquinonic constituents, as well as the observed anti-inflammatory properties of quercetin, recorded effects must be related with the presence of several bioactives.

ACS Style

Tiago Macedo; Federico Ferreres; David M. Pereira; Andreia P. Oliveira; Nelson G.M. Gomes; Ángel Gil-Izquierdo; Patrícia Valentão; Luísa Araújo; Paula B. Andrade. Cassia sieberiana DC. leaves modulate LPS-induced inflammatory response in THP-1 cells and inhibit eicosanoid-metabolizing enzymes. Journal of Ethnopharmacology 2020, 269, 113746 .

AMA Style

Tiago Macedo, Federico Ferreres, David M. Pereira, Andreia P. Oliveira, Nelson G.M. Gomes, Ángel Gil-Izquierdo, Patrícia Valentão, Luísa Araújo, Paula B. Andrade. Cassia sieberiana DC. leaves modulate LPS-induced inflammatory response in THP-1 cells and inhibit eicosanoid-metabolizing enzymes. Journal of Ethnopharmacology. 2020; 269 ():113746.

Chicago/Turabian Style

Tiago Macedo; Federico Ferreres; David M. Pereira; Andreia P. Oliveira; Nelson G.M. Gomes; Ángel Gil-Izquierdo; Patrícia Valentão; Luísa Araújo; Paula B. Andrade. 2020. "Cassia sieberiana DC. leaves modulate LPS-induced inflammatory response in THP-1 cells and inhibit eicosanoid-metabolizing enzymes." Journal of Ethnopharmacology 269, no. : 113746.

Journal article
Published: 23 November 2020 in Nutrition, Metabolism and Cardiovascular Diseases
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Background and aim The overconsumption of sucrose is closely related to sugar-sweetened beverages and one of the main factors associated with the increase of metabolic diseases, such as type 2 diabetes, obesity, and insulin resistance. So, the addition of alternative sweeteners to new fruit-based drinks could contribute to minimizing the incidence or severity of these pathologies. Nevertheless, current knowledge on the influence of these additives on the bioactive compounds present in these beverages is still scarce.new-onset hypertension, but few data were published in Asian. We aimed to investigate the association of lipid profiles with new-onset hypertension in a Chinese community-based non-hypertensive cohort without lipid-lowering treatment (n = 1802). Methods and results Hence, to contribute to the understanding of this issue, the plasma concentration of phenolic compounds (anthocyanins and flavanones), after the ingestion of a new maqui-citrus-based beverage, supplemented with sucrose (natural high caloric), stevia (natural non-caloric), or sucralose (artificial non-caloric), was evaluated as evidence of their intestinal absorption and metabolism previous to renal excretion. The beverages were ingested by volunteers (n = 20) and the resulting phenolic metabolites in plasma were analyzed by UHPLC-ESI-MS/MS. A total of 13 metabolites were detected: caffeic acid sulfate, caffeic acid glucuronide, 3,4-dihydroxyfenylacetic, 3,4-dihydroxyfenylacetic sulfate. 3,4-dihydroxyfenylacetic acid di-sulfate, 3,4-dihydroxyfenylacetic di-glucuronide, 3,4-dihydroxyfenylacetic glucuronide-sulfate, trans-ferulic acid glucuronide, naringenin glucuronide, vanillic acid, vanillic acid sulfate, vanillic acid glucuronide-sulfate, and vanillic acid di-glucuronide, being recorded their maximum concentration after 30–60 min. Conclusion In general, sucralose provided the greatest absorption value for most of these metabolites, followed by stevia. Due to this, the present study proposes sucralose and stevia (non-caloric sweeteners) as valuable alternatives to sucrose (high caloric sweetener), to avoid the augmented risk of several metabolic disorders.

ACS Style

Vicente Agulló; Raúl Domínguez-Perles; Cristina García-Viguera. Sweetener influences plasma concentration of flavonoids in humans after an acute intake of a new (poly)phenol-rich beverage. Nutrition, Metabolism and Cardiovascular Diseases 2020, 31, 930 -938.

AMA Style

Vicente Agulló, Raúl Domínguez-Perles, Cristina García-Viguera. Sweetener influences plasma concentration of flavonoids in humans after an acute intake of a new (poly)phenol-rich beverage. Nutrition, Metabolism and Cardiovascular Diseases. 2020; 31 (3):930-938.

Chicago/Turabian Style

Vicente Agulló; Raúl Domínguez-Perles; Cristina García-Viguera. 2020. "Sweetener influences plasma concentration of flavonoids in humans after an acute intake of a new (poly)phenol-rich beverage." Nutrition, Metabolism and Cardiovascular Diseases 31, no. 3: 930-938.

Journal article
Published: 12 October 2020 in International Journal of Environmental Research and Public Health
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Menstrual problems affect many young women worldwide, conditioning both their academic performance and quality of life. This study sought to analyse the prevalence of menstrual problems and their possible relationship with lifestyle among Spanish university women, as part of a research project (UniHcos Project) involving a cohort of 11 Spanish universities with 7208 university students. A descriptive analysis was performed using the bivariate chi-square test and the Student’s t-test together with a binary logistic regression, in which the dependent variable was ‘suffering from menstrual problems’. Menstrual problems were identified in 23.8% of the students, representing women who paid more visits to the doctor and to emergency rooms, and who consumed more painkillers and contraceptives. In relation to dietary preferences, menstrual problems were 1.39 (CI 95% 1.22–1.61; p = 0.000) times more likely among women classified as high-risk alcohol users according to the AUDIT questionnaire, and 1.187 (CI 95% 1.029–1.370; p = 0.019) times greater among those who consumed sweets daily, 1.592 (CI 95% 1.113–2.276; p = 0.011) times more frequent among those who eat fish daily, and 1.199 (CI 95% 1.004–1.432; p = 0.045) times greater among those who were dieting. Menstrual problems affect many college students and potentially modifiable lifestyle variables exist which may influence their prevalence. It would be interesting to develop programmes to promote women’s health in the university context.

ACS Style

Elia Fernández-Martínez; Tania Fernández-Villa; Carmen Amezcua-Prieto; María Morales Suárez-Varela; Ramona Mateos-Campos; Carlos Ayán-Pérez; Antonio José Molina De La Torre; Rocío Ortíz-Moncada; Ana Almaraz; Gemma Blázquez Abellán; Miguel Delgado-Rodríguez; Jéssica Alonso-Molero; Virginia Martínez-Ruíz; Agustín Llopis-Morales; Luis Félix Valero Juan; José Mª Cancela Carral; Sandra Martín-Peláez; Juan Alguacil. Menstrual Problems and Lifestyle Among Spanish University Women. International Journal of Environmental Research and Public Health 2020, 17, 7425 .

AMA Style

Elia Fernández-Martínez, Tania Fernández-Villa, Carmen Amezcua-Prieto, María Morales Suárez-Varela, Ramona Mateos-Campos, Carlos Ayán-Pérez, Antonio José Molina De La Torre, Rocío Ortíz-Moncada, Ana Almaraz, Gemma Blázquez Abellán, Miguel Delgado-Rodríguez, Jéssica Alonso-Molero, Virginia Martínez-Ruíz, Agustín Llopis-Morales, Luis Félix Valero Juan, José Mª Cancela Carral, Sandra Martín-Peláez, Juan Alguacil. Menstrual Problems and Lifestyle Among Spanish University Women. International Journal of Environmental Research and Public Health. 2020; 17 (20):7425.

Chicago/Turabian Style

Elia Fernández-Martínez; Tania Fernández-Villa; Carmen Amezcua-Prieto; María Morales Suárez-Varela; Ramona Mateos-Campos; Carlos Ayán-Pérez; Antonio José Molina De La Torre; Rocío Ortíz-Moncada; Ana Almaraz; Gemma Blázquez Abellán; Miguel Delgado-Rodríguez; Jéssica Alonso-Molero; Virginia Martínez-Ruíz; Agustín Llopis-Morales; Luis Félix Valero Juan; José Mª Cancela Carral; Sandra Martín-Peláez; Juan Alguacil. 2020. "Menstrual Problems and Lifestyle Among Spanish University Women." International Journal of Environmental Research and Public Health 17, no. 20: 7425.

Journal article
Published: 08 October 2020 in Food Chemistry
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Caryota urens L. has long been valued as a traditional food, the edible fruits being eaten raw and the inflorescences commonly used on sweet sap and flour production. In the current work, the phenolic profile of methanol extracts obtained from the inflorescences and fruits was unveiled for the first time, nine caffeic acid derivatives being identified and quantified. Since kitul products have been reported for their antidiabetic properties, extracts radical scavenging activity and α-amylase, α-glucosidase and aldose reductase inhibitory activity were assessed. The inflorescences’ extract was particularly active against yeast α-glucosidase (IC50 = 1.53 μg/mL), acting through a non-competitive inhibitory mechanism. This activity was also observed in enzyme-enriched homogenates obtained from human Caco-2 cells (IC50 = 64.75 µg/mL). Additionally, the extract obtained from the inflorescences showed no cytotoxicity on HepG2, AGS and Caco-2 cell lines. Our data suggest that C. urens inflorescences can support the development of new functional foods with α-glucosidase inhibitory activity.

ACS Style

Federico Ferreres; Catarina Andrade; Nelson G.M. Gomes; Paula B. Andrade; Angel Gil-Izquierdo; David M. Pereira; Rungcharn Suksungworn; Sutsawat Duangsrisai; Romeu A. Videira; Patrícia Valentão. Valorisation of kitul, an overlooked food plant: Phenolic profiling of fruits and inflorescences and assessment of their effects on diabetes-related targets. Food Chemistry 2020, 342, 128323 .

AMA Style

Federico Ferreres, Catarina Andrade, Nelson G.M. Gomes, Paula B. Andrade, Angel Gil-Izquierdo, David M. Pereira, Rungcharn Suksungworn, Sutsawat Duangsrisai, Romeu A. Videira, Patrícia Valentão. Valorisation of kitul, an overlooked food plant: Phenolic profiling of fruits and inflorescences and assessment of their effects on diabetes-related targets. Food Chemistry. 2020; 342 ():128323.

Chicago/Turabian Style

Federico Ferreres; Catarina Andrade; Nelson G.M. Gomes; Paula B. Andrade; Angel Gil-Izquierdo; David M. Pereira; Rungcharn Suksungworn; Sutsawat Duangsrisai; Romeu A. Videira; Patrícia Valentão. 2020. "Valorisation of kitul, an overlooked food plant: Phenolic profiling of fruits and inflorescences and assessment of their effects on diabetes-related targets." Food Chemistry 342, no. : 128323.

Journal article
Published: 16 September 2020 in Food Research International
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Notwithstanding Gustavia gracillima Miers widespread distribution in neotropical regions, its chemical profile and biological properties remain uninvestigated. A methanol extract obtained from the flowers was characterized through HPLC-DAD-ESI/MSn, nine ellagic acid derivatives and twelve kaempferol 3-O-glycosides being identified and quantitated for the first time at the species and genus. Preliminary cytotoxicity screening did not reveal noticeable effects upon the gastrointestinal representative cell lines (AGS, Caco-2 and Hep G2) which further prompted us to evaluate the impact in a series of targets involved in metabolic disorders and associated complications. Despite of the moderate inhibition towards 5-lipoxygense activity, G. gracillima methanol extract displayed significant effects on carbohydrates-hydrolysing enzymes. In contrast with the antidiabetic reference drug acarbose, the extract was able to selectively inhibit yeast α-glucosidase activity (IC50 = 4.72 µg/mL), with negligible inhibitory effects upon α-amylase. Kinetic studies pointed to a model of mixed inhibition with a great binding activity, characterized by an inhibitory constant of 2.91 µg/mL. The notable inhibitory activity was also confirmed in α-glucosidase homogenates isolated from human intestinal cells (IC50 = 34.03 µg/mL). Moreover, the extract obtained from the flowers of G. gracillima displayed significant aldose reductase inhibition (IC50 = 61.88 µg/mL), as well as O2•- and •NO scavenging properties. A moderate inhibitory effect was also recorded against pancreatic lipase (IC50 = 362.17 µg/mL) through a mixed inhibition mode. Recorded data supports the potential incorporation of G. gracillima flowers on antidiabetic herbal formulations and/or supplements, with not only straight action on carbohydrates digestion, but also direct interference with targets involved on subsequent events, such as triglycerides metabolism, inflammation and radical-mediated stress.

ACS Style

Catarina Andrade; Federico Ferreres; Nelson G.M. Gomes; Angel Gil-Izquierdo; Sorawit Bapia; Sutsawat Duangsrisai; David M. Pereira; Paula B. Andrade; Patrícia Valentão. Gustavia gracillima Miers. flowers effects on enzymatic targets underlying metabolic disorders and characterization of its polyphenolic content by HPLC-DAD-ESI/MS. Food Research International 2020, 137, 109694 .

AMA Style

Catarina Andrade, Federico Ferreres, Nelson G.M. Gomes, Angel Gil-Izquierdo, Sorawit Bapia, Sutsawat Duangsrisai, David M. Pereira, Paula B. Andrade, Patrícia Valentão. Gustavia gracillima Miers. flowers effects on enzymatic targets underlying metabolic disorders and characterization of its polyphenolic content by HPLC-DAD-ESI/MS. Food Research International. 2020; 137 ():109694.

Chicago/Turabian Style

Catarina Andrade; Federico Ferreres; Nelson G.M. Gomes; Angel Gil-Izquierdo; Sorawit Bapia; Sutsawat Duangsrisai; David M. Pereira; Paula B. Andrade; Patrícia Valentão. 2020. "Gustavia gracillima Miers. flowers effects on enzymatic targets underlying metabolic disorders and characterization of its polyphenolic content by HPLC-DAD-ESI/MS." Food Research International 137, no. : 109694.

Journal article
Published: 31 July 2020 in Free Radical Biology and Medicine
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Oxylipins are considered biomarkers related to cardiovascular diseases (CVDs). They are generated in vivo via the oxygenation of polyunsaturated fatty acids as a result of oxidative stress and inflammation. Oxylipins are involved in vascular functions and are produced during foam cell formation in atherogenesis. Additionally, the consumption coffee is associated with the regulation on a particular oxylipin group, the F2t-isoprostanes (F2t-IsoPs). This function has been attributed to the chlorogenic acids (CGAs) from the coffee beverage. Considering the anti-inflammatory and antioxidant properties of CGAs, we evaluated the effects of two types of coffee that provided 787 mg CGAs/day (Coffee A) and 407 mg CGAs/day (Coffee B) by reducing 35 selected oxylipins in healthy subjects. Furthermore, we assessed the effect of CGAs on the cellular proatherogenic response in foam cells by using an oxidized LDL (oxLDL)-macrophage interaction model. After eight weeks of coffee consumption, the contents of 12 urine oxylipins were reduced. However, the effect of Coffee A showed a stronger decrease in IsoPs, dihomo-IsoPs, prostaglandins (PGs) and PG metabolites, probably due to its higher content of CGAs. Neither of the two coffees reduced the levels of oxLDL. Moreover, the in vitro oxylipin induction by oxLDL on foam cells was ameliorated by phenolic acids and CGAs, including the inhibition of IsoPs and PGs by caffeoylquinic and dicaffeoylquinic acids, respectively, while the phenolic acids maintained both antioxidant and anti-inflammatory activities. These findings suggest that coffee antioxidants are strong regulators of oxylipins related to CVDs. The clinical trial was registered on the International Clinical Trials Registry Platform, WHO primary registry (RPCEC00000168).

ACS Style

Oscar Javier Lara-Guzman; Sonia Medina; Rafael Álvarez; Camille Oger; Thierry Durand; Jean-Marie Galano; Natalia Zuluaga; Ángel Gil-Izquierdo; Katalina Muñoz-Durango. Oxylipin regulation by phenolic compounds from coffee beverage: Positive outcomes from a randomized controlled trial in healthy adults and macrophage derived foam cells. Free Radical Biology and Medicine 2020, 160, 604 -617.

AMA Style

Oscar Javier Lara-Guzman, Sonia Medina, Rafael Álvarez, Camille Oger, Thierry Durand, Jean-Marie Galano, Natalia Zuluaga, Ángel Gil-Izquierdo, Katalina Muñoz-Durango. Oxylipin regulation by phenolic compounds from coffee beverage: Positive outcomes from a randomized controlled trial in healthy adults and macrophage derived foam cells. Free Radical Biology and Medicine. 2020; 160 ():604-617.

Chicago/Turabian Style

Oscar Javier Lara-Guzman; Sonia Medina; Rafael Álvarez; Camille Oger; Thierry Durand; Jean-Marie Galano; Natalia Zuluaga; Ángel Gil-Izquierdo; Katalina Muñoz-Durango. 2020. "Oxylipin regulation by phenolic compounds from coffee beverage: Positive outcomes from a randomized controlled trial in healthy adults and macrophage derived foam cells." Free Radical Biology and Medicine 160, no. : 604-617.

Original contribution
Published: 29 July 2020 in European Journal of Nutrition
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Purpose Coffee is an important source of bioactive compounds, including caffeine, trigonelline, and phenolic compounds. Several studies have highlighted the preventive effects of coffee consumption on major cardiometabolic (CM) diseases, but the impact of different coffee dosages on markers of CM risk in a real-life setting has not been fully understood. This study aimed to investigate the effect of coffee and cocoa-based confectionery containing coffee consumption on several CM risk factors in healthy subjects. Methods In a three-arm, crossover, randomized trial, 21 volunteers were assigned to consume in a random order for 1 month: 1 cup of espresso coffee/day, 3 cups of espresso coffee/day, and 1 cup of espresso coffee plus 2 cocoa-based products containing coffee, twice per day. At the last day of each treatment, blood samples were collected and used for the analysis of inflammatory markers, trimethylamine N-oxide, nitric oxide, blood lipids, and markers of glucose/insulin metabolism. Moreover, anthropometric parameters and blood pressure were measured. Finally, food consumption during the interventions was monitored. Results After 1 month, energy intake did not change among treatments, while significant differences were observed in the intake of saturated fatty acids, sugars, and total carbohydrates. No significant effect on CM markers was observed following neither the consumption of different coffee dosages nor after cocoa-based products containing coffee. Conclusions The daily consumption of common dosages of coffee and its substitution with cocoa-based products containing coffee showed no effect on CM risk factors in healthy subjects. Trial registration number Registered at clinicaltrials.gov as NCT03166540, May 21, 2017.

ACS Style

Daniela Martini; Alice Rosi; Michele Tassotti; Monica Antonini; Margherita Dall’Asta; Letizia Bresciani; Federica Fantuzzi; Valentina Spigoni; Raúl Domínguez-Perles; Donato Angelino; Cristian Ricci; Soledad Del Pozo-Luengo; Pedro Luis Tornel; Francesca Scazzina; Angel Gil-Izquierdo; Alessandra Dei Cas; Furio Brighenti; Riccardo Bonadonna; Daniele Del Rio; Pedro Mena. Effect of coffee and cocoa-based confectionery containing coffee on markers of cardiometabolic health: results from the pocket-4-life project. European Journal of Nutrition 2020, 60, 1453 -1463.

AMA Style

Daniela Martini, Alice Rosi, Michele Tassotti, Monica Antonini, Margherita Dall’Asta, Letizia Bresciani, Federica Fantuzzi, Valentina Spigoni, Raúl Domínguez-Perles, Donato Angelino, Cristian Ricci, Soledad Del Pozo-Luengo, Pedro Luis Tornel, Francesca Scazzina, Angel Gil-Izquierdo, Alessandra Dei Cas, Furio Brighenti, Riccardo Bonadonna, Daniele Del Rio, Pedro Mena. Effect of coffee and cocoa-based confectionery containing coffee on markers of cardiometabolic health: results from the pocket-4-life project. European Journal of Nutrition. 2020; 60 (3):1453-1463.

Chicago/Turabian Style

Daniela Martini; Alice Rosi; Michele Tassotti; Monica Antonini; Margherita Dall’Asta; Letizia Bresciani; Federica Fantuzzi; Valentina Spigoni; Raúl Domínguez-Perles; Donato Angelino; Cristian Ricci; Soledad Del Pozo-Luengo; Pedro Luis Tornel; Francesca Scazzina; Angel Gil-Izquierdo; Alessandra Dei Cas; Furio Brighenti; Riccardo Bonadonna; Daniele Del Rio; Pedro Mena. 2020. "Effect of coffee and cocoa-based confectionery containing coffee on markers of cardiometabolic health: results from the pocket-4-life project." European Journal of Nutrition 60, no. 3: 1453-1463.

Research article
Published: 21 July 2020 in Journal of Agricultural and Food Chemistry
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Even though traditionally date-fruit has been featured by a marginal use, mainly restricted to its dietary intake, in recent years, it has raised the range of applications for this agro-food production.. These new uses have entailed an enlarged production of date fruits and, simultaneously, of date palm by-products. Encouraged by the traditional medicinal uses of dates, according to their phytochemical composition, the present work was focused on the evaluation of a new family of secondary metabolites, the plant oxylipins phytoprostanes (PhytoPs) and phytofurans (PhytoFs), in six separate matrices of the date palm edible parts and by-products, applying an UHPLC-ESI-QqQ-MS/MS-based methodology. The evaluation for the first time of date palm edible parts and by-products as a dietary source of PhytoPs and PhytoFs provides evidence on the value of six different parts (pulp, skin, pits, leaves, clusters, and pollen) regarding their content in these plant oxylipins evidenced the presence of the PhytoPs, 9-F1t-PhytoP (201.3-7223.1 ng/100 g dw) and 9-epi-9-F1t-PhytoP (209.7-7297.4 ng/100 g dw), and the PhytoFs ent-16(RS)-9-epi-ST-Δ14-10-PhytoF (4.6-191.0 ng/100g dw) and ent-16(RS)-13-epi-ST-Δ14-9-PhytoF as the most abundant compounds. Regarding the diverse matrices assessed, pollen, clusters, and leaves for PhytoPs and skins and pollen for PhytoFs were identified as the most interesting sources of these compounds. In this concern, the information obtained upon the detailed characterization performed in the present work will allow unravelling the biological interest of PhytoPs and PhytoFs and the extent to which these compounds could exert valuable biological activities upon in vitro (mechanistic) and in vivo studies, allocating the effort-focus on the chemical species of PhytoPs and PhytoFs responsible for such traits.

ACS Style

Sonia Medina; Ángel Gil-Izquierdo; Ibrahim M. Abu-Reidah; Thierry Durand; Valérie Bultel-Poncé; Jean-Marie Galano; Raúl Domínguez-Perles. Evaluation of Phoenix dactylifera Edible Parts and Byproducts as Sources of Phytoprostanes and Phytofurans. Journal of Agricultural and Food Chemistry 2020, 68, 8942 -8950.

AMA Style

Sonia Medina, Ángel Gil-Izquierdo, Ibrahim M. Abu-Reidah, Thierry Durand, Valérie Bultel-Poncé, Jean-Marie Galano, Raúl Domínguez-Perles. Evaluation of Phoenix dactylifera Edible Parts and Byproducts as Sources of Phytoprostanes and Phytofurans. Journal of Agricultural and Food Chemistry. 2020; 68 (33):8942-8950.

Chicago/Turabian Style

Sonia Medina; Ángel Gil-Izquierdo; Ibrahim M. Abu-Reidah; Thierry Durand; Valérie Bultel-Poncé; Jean-Marie Galano; Raúl Domínguez-Perles. 2020. "Evaluation of Phoenix dactylifera Edible Parts and Byproducts as Sources of Phytoprostanes and Phytofurans." Journal of Agricultural and Food Chemistry 68, no. 33: 8942-8950.

Research article
Published: 19 July 2020 in Journal of Agricultural and Food Chemistry
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Pistachio (Pistacia vera L.) is a drought-tolerant specie grown under the semiarid conditions of the Mediterranean basin. For this reason, it is essential to make an exhaustive quantification of yield and quality benefits of the kernels because the regulated deficit irrigation will allow significant water savings with a minimum impact on yield, while improving kernel quality. The goal of this scientific work was the study of the influence of the rootstock, water deficit during pit hardening and kernel roasting on pistachio (Pistacia vera, cv. Kerman) fruit yield, fruit size, and kernel content of fatty acids, phytoprostanes (PhytoPs) and phytofurans (PhytoFs) for the first time. Water stress during pit hardening did not affect the pistachio yield. The kernels cultivar showed a lower oleic acid and a higher linoleic acid contents than those found for other cultivars. Kernels from plants grafted on the studied rootstocks showed very interesting characteristics. P. integerrima led to the highest percentage of monounsaturated fatty acids. Regarding the plant oxylipins, P. terebinthus led to the highest contents of PhytoPs and PhytoFs (1260 ng/100g and 16.2 ng/100g, respectively). In addition, nuts from trees cultivated under intermediate water deficit during pit hardening showed increased contents of the 9-series of F1-phytoprostanes and ent-16(RS)-9-epi-ST-Δ14-10-phytofuran. However, roasting of pistachios led to PhytoPs degradation. Therefore, plant cultivar, deficit irrigation, rootstock, and roasting must be considered to enhance biosynthesis of these secondary metabolites. New tools using agricultural strategies to produce hydroSOS pistachios have been opened thanks to the biological properties of these prostaglandins-like compounds linking agriculture, nutrition and food science technology for further research initiatives.

ACS Style

Jacinta Collado; Marina Cano-Lamadrid; David Pérez-López; Ángel A. Carbonell-Barrachina; Ana Centeno; Sonia Medina; Isabel Grinana; Alexandre Guy; Jean-Marie Galano; Thierry Durand; Raúl Domínguez-Perles; Arturo Torrecillas; Federico Ferreres; Ángel Gil-Izquierdo. Effects of Deficit Irrigation, Rootstock, and Roasting on the Contents of Fatty Acids, Phytoprostanes, and Phytofurans in Pistachio Kernels. Journal of Agricultural and Food Chemistry 2020, 68, 8915 -8924.

AMA Style

Jacinta Collado, Marina Cano-Lamadrid, David Pérez-López, Ángel A. Carbonell-Barrachina, Ana Centeno, Sonia Medina, Isabel Grinana, Alexandre Guy, Jean-Marie Galano, Thierry Durand, Raúl Domínguez-Perles, Arturo Torrecillas, Federico Ferreres, Ángel Gil-Izquierdo. Effects of Deficit Irrigation, Rootstock, and Roasting on the Contents of Fatty Acids, Phytoprostanes, and Phytofurans in Pistachio Kernels. Journal of Agricultural and Food Chemistry. 2020; 68 (33):8915-8924.

Chicago/Turabian Style

Jacinta Collado; Marina Cano-Lamadrid; David Pérez-López; Ángel A. Carbonell-Barrachina; Ana Centeno; Sonia Medina; Isabel Grinana; Alexandre Guy; Jean-Marie Galano; Thierry Durand; Raúl Domínguez-Perles; Arturo Torrecillas; Federico Ferreres; Ángel Gil-Izquierdo. 2020. "Effects of Deficit Irrigation, Rootstock, and Roasting on the Contents of Fatty Acids, Phytoprostanes, and Phytofurans in Pistachio Kernels." Journal of Agricultural and Food Chemistry 68, no. 33: 8915-8924.

Journal article
Published: 15 July 2020 in Microorganisms
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Over the last 20 years, Lactobacillus species inhabiting the gastrointestinal tract (GIT) have received much attention, and their health-promoting properties are now well-described. Probiotic effects cannot be generalized, and their uses cover a wide range of applications. It is thus important to proceed to an accurate selection and evaluation of probiotic candidates. We evaluate the probiotic potential of six strains of Lactobacillus in different in vitro models representing critical factors of either survival, efficacy, or both. We characterized the strains for their ability to (i) modulate intestinal permeability using transepithelial electrical resistance (TEER), (ii) form biofilms and resist stressful conditions, and (iii) produce beneficial host and/or bacteria metabolites. Our data reveal the specificity of Lactobacillus strains to modulate intestinal permeability depending on the cell type. The six isolates were able to form spatially organized biofilms, and we provide evidence that the biofilm form is beneficial in a strongly acidic environment. Finally, we demonstrated the ability of the strains to produce γ-aminobutyric acid (GABA) that is involved in the gut-brain axis and beneficial enzymes that promote the bacterial tolerance to bile salts. Overall, our study highlights the specific properties of Lactobacillus strains and their possible applications as biofilms.

ACS Style

Célia Chamignon; Virgile Guéneau; Sonia Medina; Julien Deschamps; Angel Gil-Izquierdo; Romain Briandet; Pierre-Yves Mousset; Philippe Langella; Sophie Lafay; Luis G. Bermúdez-Humarán. Evaluation of the Probiotic Properties and the Capacity to Form Biofilms of Various Lactobacillus Strains. Microorganisms 2020, 8, 1053 .

AMA Style

Célia Chamignon, Virgile Guéneau, Sonia Medina, Julien Deschamps, Angel Gil-Izquierdo, Romain Briandet, Pierre-Yves Mousset, Philippe Langella, Sophie Lafay, Luis G. Bermúdez-Humarán. Evaluation of the Probiotic Properties and the Capacity to Form Biofilms of Various Lactobacillus Strains. Microorganisms. 2020; 8 (7):1053.

Chicago/Turabian Style

Célia Chamignon; Virgile Guéneau; Sonia Medina; Julien Deschamps; Angel Gil-Izquierdo; Romain Briandet; Pierre-Yves Mousset; Philippe Langella; Sophie Lafay; Luis G. Bermúdez-Humarán. 2020. "Evaluation of the Probiotic Properties and the Capacity to Form Biofilms of Various Lactobacillus Strains." Microorganisms 8, no. 7: 1053.

Research article
Published: 30 June 2020 in Journal of Agricultural and Food Chemistry
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Lipophenols have been stressed as an emerging class of functional compounds. However, little is known about their diversity. Thus, this study is aimed at developing a new method for the extraction, clean-up, and UHPLC-ESI-QqQ-MS/MS-based analysis of the lipophenols derived from hydroxytyrosol (HT): α-linolenic (HT-ALA), linoleic acid (HT-LA), and oleic acid (HT-OA). The method validated provides reliable analytical data and practical applications. It was applied to an array of oily (extra virgin olive oil, refined olive oil, flaxseed oil, grapeseed oil, and margarine) and aqueous (pineapple juice) matrices, non-fortified and fortified with HT. Also, the present work reported the formation of fatty acids esters of HT (HT-FAs) that seem to be closely dependent on the fatty acid profile of the food matrix, encouraging the further exploration of the theoretical basis for the generation of HT-FAs, as well as their contribution to the healthy attributions of plant-based foods.

ACS Style

Sonia Medina; Raúl Domínguez-Perles; David Auñón; Espérance Moine; Thierry Durand; Céline Crauste; Federico Ferreres; Ángel Gil-Izquierdo. Targeted Lipidomics Profiling Reveals the Generation of Hydroxytyrosol-Fatty Acids in Hydroxytyrosol-Fortified Oily Matrices: New Analytical Methodology and Cytotoxicity Evaluation. Journal of Agricultural and Food Chemistry 2020, 68, 7789 -7799.

AMA Style

Sonia Medina, Raúl Domínguez-Perles, David Auñón, Espérance Moine, Thierry Durand, Céline Crauste, Federico Ferreres, Ángel Gil-Izquierdo. Targeted Lipidomics Profiling Reveals the Generation of Hydroxytyrosol-Fatty Acids in Hydroxytyrosol-Fortified Oily Matrices: New Analytical Methodology and Cytotoxicity Evaluation. Journal of Agricultural and Food Chemistry. 2020; 68 (29):7789-7799.

Chicago/Turabian Style

Sonia Medina; Raúl Domínguez-Perles; David Auñón; Espérance Moine; Thierry Durand; Céline Crauste; Federico Ferreres; Ángel Gil-Izquierdo. 2020. "Targeted Lipidomics Profiling Reveals the Generation of Hydroxytyrosol-Fatty Acids in Hydroxytyrosol-Fortified Oily Matrices: New Analytical Methodology and Cytotoxicity Evaluation." Journal of Agricultural and Food Chemistry 68, no. 29: 7789-7799.