Nondestructive methods to estimate individual leaf area (LA) accurately, by leaf length (L) and/or width (W), is helpful for the in situ and successive LA measurements. However, leaf shape and size may covary with environment and thus alter the coefficients of LA estimation models. To test such hypothesis, we carried out an experiment by measuring Saussurea stoliczkai C. B. Clarke leaves along an altitudinal transect in Damxung county, central Tibet. In July 2011, we selected seven sites at about every 150 m in altitude from 4,350 m to 5,250 m a.s.l. A total of 1,389 leaves (182 to 203 leaves for each site) were measured. For each site, models developed by two leaf dimensions [LA = a (L×W) + b] could estimate LA more accurately than those by single dimension. L, W, LA and leaf shape index (L:W ratio) all decreased with increasing altitude, leading to significant differences in coefficients of two-dimension model between almost every two sites. Accordingly, a common two-dimension model is unlikely to occur for S. stoliczkai across the whole altitudinal transect, indicating that the varying leaf shape may alter the coefficient of LA estimation models.

Introduction: Shrub promotes the survival, growth and reproduction of understory species by buffering the environmental extremes and improving limited resources (i.e., facilitation effect) in arid and semiarid regions. However, the importance of soil water and nutrient availability on shrub facilitation, and its trend along a drought gradient have been relatively less addressed in water-limited systems.Methods: We investigated species richness, plant size, soil total nitrogen and dominant grass leaf δ¹³C within and outside the dominant leguminous cushion-like shrub Caragana versicolor along a water deficit gradient in drylands of Tibetan Plateau.Results: We found that C. versicolor increased grass species richness but had a negative effect on annual and perennial forbs. Along the water deficit gradient, plant interaction assessed by species richness (RII_species) showed a unimodal pattern with shift from increase to decrease, while plant interaction assessed by plant size (RII_size) did not vary significantly. The effect of C. versicolor on soil nitrogen, rather than water availability, determined its overall effect on understory species richness. Neither the effect of C. versicolor on soil nitrogen nor water availability affected plant size.Discussion: Our study suggests that the drying tendency in association with the recent warming trends observed in drylands of Tibetan Plateau, will likely hinder the facilitation effect of nurse leguminous shrub on understories if moisture availability crosses a critical minimum threshold.

As the oldest species of Betulaceae, Nepalese alder (Alnus nepalensis) shows a high capacity for nitrogen fixation, rapid growth rate, and strong adaptability to stress environments, and it plays an important role in maintaining the structure and function of forest and agroforestry ecosystems. We explored its geographic distribution and the corresponding environmental drivers through collecting specimen records and published literature for Nepalese alder over the world during the past 40 years. The research trends, the growth limiting factors, the physiological characteristics, and ecological functions were all summarized as well. In terms of geographical distribution and limiting factors, Nepalese alder is mainly distributed in southern mountainous areas of the Himalayas and southwest China. Since it presented a clear northern limit of distribution and an upper limit of elevation, temperature is assumed to be the main environmental limiting factor. According to historical development, the research history of Nepalese alder could be divided into three main periods: the initial development (before 2001), the fast development (2002–2015), and the high-quality development (2016–2022), with the two key points in 2002 and 2015 relating to the conversion of cropland to a forest project that the government conducted and the application from theory to practice, respectively. As can be seen from the ecological functions, Nepalese alder could form symbiotic nodules with Frankia, which plays an important role in improving soil physical and chemical properties and facilitating vegetation secondary succession. Overall, the present review provides a reference for further studies on ecological adaptability and sustainable utilization of Nepalese alder under climate change, and also for regional ecosystem service, forestry production practice, and vegetation restoration.

A better understanding of the structure and dynamics of disturbed forests is key for forecasting their future successional trajectories. Despite vulnerability of subalpine forests to warming climate, little is known as to how their community composition has responded to disturbances and climate warming over decades. Before the 1970s, subalpine forests on the southeastern Qinghai-Tibet Plateau mainly experienced logging and fire, but afterwards they were more impacted by climate warming. Thus, they provide an excellent setting to test whether disturbances and climate warming led to changes in forest structure. Based on the analysis of 3145 forest inventory plots at 4- to 5-year resolution, we found that spruce-fir forests shifted to pine and broadleaved forests since the early 1970s. Such a turnover in species composition mainly occurred in the 1994–1998 period. By strongly altering site conditions, disturbances in concert with climate warming reshuffle community composition to warm-adapted broadleaf-pine species. Thus, moderate disturbances shifted forest composition through a gradual loss of resilience of spruce-fir forests. Shifts in these foundation species will have profound impacts on ecosystem functions and services. In the future, broadleaved forests could expand more rapidly than evergreen needle-leaved forests under moderate warming scenarios. In addition to climate, the effects of anthropogenic disturbances on subalpine forests should be considered in adaptive forest management and in projections of future forest changes.