Objective: Laser treatment has been recently introduced in many fields of implant dentistry. The systematic review tried to address the question: “How does laser modification of titanium surface influence fibroblast adhesion?”. Methods: An electronic search of the PubMed and Scopus databases was performed. The following keywords were used: (laser) AND (fibroblast) AND (titanium) AND (implant OR disc) AND (proliferation OR adhesion). Initially, 136 studies were found. Ten studies met the inclusion criteria and were included in the review. All studies chosen to be included in the review were considered to have a low risk of bias. Results: Studies included in the review varied with laser parameters or ways of observing fibroblast behavior. Studies showed that fibroblasts tend to take different shapes and create extensions on modified surfaces and that their metabolic activity is more intense. One study concentrated on laser application and showed that three-directional laser application is the most successful in terms of fibroblast adhesion. Studies which concentrated more on laser parameters showed that too low energy density (lower or equal to 0.75 J/cm²) does not influence fibroblast adhesion. Increasing the energy density over 0.75 J/cm² causes better cell adhesion of fibroblasts to the laser-modified sample. One included study focused on increasing titanium surface wettability, which also positively influenced cell adhesion. Conclusion: The studies included in the review proved a positive effect of laser-modified titanium surfaces on fibroblast adhesion. However, the application of an appropriate laser energy dose is crucial.

Molecular methods have become integral to microbiological research for microbial identification. This literature review focuses on the application of molecular methods in examining airborne bacteria and fungi in healthcare facilities. In January 2024, a comprehensive electronic search was carried out in esteemed databases including PubMed, Web of Science, and Scopus, employing carefully selected keywords such as ((bacteria) OR (virus) OR (fungi)) AND (aerosol) AND ((hospital) OR (healthcare) OR (dental office)) AND ((molecular) OR (PCR) OR (NGS) OR (RNA) OR (DNA) OR (metagenomic) OR (microarray)), following the PRISMA protocol. The review specifically targets healthcare environments with elevated concentrations of pathogenic bacteria. A total of 487 articles were initially identified, but only 13 met the inclusion criteria and were included in the review. The study disclosed that the prevalent molecular methodology for appraising aerosol quality encompassed the utilization of the PCR method, incorporating either 16S rRNA (bacteria) or 18S rRNA (fungi) amplification techniques. Notably, five diverse molecular techniques, specifically PFGE, DGGE, SBT, LAMP, and DNA hybridization methods, were implemented in five distinct studies. These molecular tests exhibited superior capabilities compared to traditional bacterial and fungal cultures, providing precise strain identification. Additionally, the molecular methods allowed the detection of gene sequences associated with antibiotic resistance. In conclusion, molecular testing offers significant advantages over classical microbiological culture, providing more comprehensive information.

Objective: Esthetic dentistry is an important factor in increasing patients’ quality of life. This study aimed to investigate the impact of laser use on bleaching procedures for natural teeth and dental restorative materials. Methods: In January 2024, an electronic search was conducted using PubMed, Web of Science (WoS), and Scopus databases with the keywords (tooth) AND (laser) AND (bleaching), following PRISMA guidelines and the PICO framework. The initial search yielded 852 articles, of which 441 were screened. After applying inclusion criteria, 376 articles were excluded as they did not focus on the use of lasers in bleaching natural teeth and restorative materials. Consequently, 40 articles were included in the final review. Results: Of the 40 qualified publications, 29 utilized a diode laser, of which 10 authors concluded that it increases the whitening effect comparing classical methods. Three of included publications investigated the whitening of dental materials, while another three focused on endodontically treated teeth. Whitening procedures on ceramics effectively removed discoloration, but the resulting color did not significantly differ from the initial shade. Conversely, composite materials not only failed to bleach but also exhibited altered physical properties, thereby increasing their susceptibility to further discoloration. The KTP laser demonstrated promising outcomes on specific stains. The Er,Cr:YSGG and Er:YAG lasers also showed beneficial effects, although there were variations in their efficacy and required activation times. Conclusions: The findings partially indicate that laser-assisted bleaching improves the whitening of natural teeth. Further research on the effect of laser bleaching on the physical parameters of restorative materials is necessary.

Objective: This systematic review aims to evaluate the impact of incorporating chitosan into dental resin composites on their mechanical, antibacterial, and physicochemical properties. Methods: A comprehensive search of PubMed, Scopus, and Web of Science databases was conducted in March 2025 using the following keywords: resin, composite, and chitosan. The inclusion criteria comprised in vitro studies in dentistry evaluating chitosan as a composite additive with full-text availability in English. Data extraction and quality assessment were performed independently by multiple reviewers using standardized tools, and study quality was assessed based on predefined criteria. Results: Seventeen studies met the inclusion criteria. Chitosan concentrations in the composites ranged from 0.25 wt% to 20 wt%. Antibacterial activity was confirmed in six studies, especially against S. mutans, S. sanguinis, and L. acidophilus. Mechanical properties such as fracture toughness, hardness, and compressive strength were generally improved at lower concentrations of chitosan. However, increased chitosan levels were associated with decreased flexural strength and increased microleakage. Shear bond strength (SBS) was unaffected by chitosan in low concentrations (up to 0.25%), while higher concentrations reduced SBS. Fluoride release capacity was assessed in one study, with no significant differences observed. Conclusion: Chitosan-modified dental resin composites exhibit promising antibacterial and mechanical enhancements at low concentrations. However, higher chitosan levels may compromise certain mechanical and adhesive properties. These findings suggest a need for standardized methodologies and further research on long-term clinical implications and fluoride release.

Cephalometry is a crucial examination in orthodontic diagnostics and during the planning of orthognathic surgical procedures. The objective of this article was to compare cephalometric measurements conducted by different specialists and systems tailored for such measurements, as well as to evaluate the capabilities of artificial intelligence in this field. In January 2024, we conducted electronic searches in the PubMed, Scopus, and Web of Science (WoS) databases. In the Scopus database, the results were refined to titles, abstracts, and keywords, while in PubMed, they were narrowed down to titles and abstracts. In WoS, the results were refined only to abstracts. The search criteria were based on the following terms: (cephalometric) AND (analysis) AND (discrepancy) AND ((orthodontic) OR (radiologist)). A total of 263 articles were identified, of which 17 met the criteria and were incorporated into the review. The review allowed us to conclude that the accuracy of cephalometric measurements relied on the expertise of the operator—specialists with more experience exhibited greater precision compared to novices or individuals not specialized in orthodontics. Cephalometric measurement computer programs yielded outcomes that streamlined work processes, minimized human errors, and enhanced precision. A novel aspect involved the application of artificial intelligence, which also demonstrated high precision and a substantial reduction in working time, although its utilization still necessitates further enhancements. Further research was required to address these limitations and to optimize the incorporation of technology in orthodontic and orthognathic surgery practices.

Background: Fluoride is vital in dentistry for caries prevention, enhancing remineralization, and inhibiting bacteria. Incorporating fluoride into restorative materials like glass-ionomer cements, compomers, and giomers has significantly increased fluoride availability in the oral cavity. This review assesses how surface coatings influence fluoride release from various dental restorative materials. Methods: In December 2023, we conducted electronic searches in PubMed, Scopus, and Web of Science (WoS) databases. In the Scopus database, the results were refined to titles, abstracts, and keywords, while in PubMed, they were narrowed down to titles and abstracts. In WoS, the results were refined only to abstracts. The search criteria were based on the terms fluoride AND release AND (coating OR glaze OR layer OR film OR varnish) AND (composite OR glass OR compomer), following PRISMA guidelines and the PICO framework. Twenty-three studies were rigorously selected and analyzed for fluoride release from coated versus uncoated materials. Results: Surface coatings typically reduce the rate of fluoride release. Glass-ionomer cements had the highest release, followed by giomers and compomers. The initial release was greater in uncoated materials but stabilized over time, influenced by variables like artificial saliva and deionized water. Conclusions: Surface coatings generally decrease fluoride release rates from dental materials. Although initial rates are high, contributing to caries prevention, more standardized research is needed to better understand the impact of coatings and optimize materials for maximum preventive benefits.