The zinc oxide–zirconium dioxide (ZnO-ZrO₂) heterojunction was prepared by a green method using rubber leaves as reducing and capping agents. Various physicochemical techniques were used to study the chemical composition and the structural and optical properties of the synthesized nanocomposite. The nature of the heterojunction was confirmed through X-ray diffraction and the average sizes of ZnO and ZrO₂ crystallites were found to be 70 and 24 nm, respectively. The photocatalytic potential of the ZnO-ZrO₂ heterojunction was examined against rhodamine 6G (Rh-6G), and 97.30 percent of the dye was degraded due to the synergistic effect of the light and the catalyst. The commercial ZnO nanopowder was used as a reference catalyst and 86.32 percent degradation was noted under the same reaction conditions. The in vitro antioxidant activity was also performed to scavenge the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals, where the activity of the ZnO-ZrO₂ heterojunction was found to be higher than the ascorbic acid.

The cucumber (Cucumis sativus L.) is one of the most important vegetables in Bangladesh as well as across the globe. However, many of the important cucumber landraces have disappeared in Bangladesh due to climate change, particularly erratic rainfall, extreme temperature, salinity, and drought. Therefore, to protect against the extinction of the cucumber landraces, we collected 103 landraces in different geographical regions of Bangladesh, including drought and saline-prone areas, and studied their divergence for the future breeding programme for the development of cultivars suitable for the climate-changing situations. Data on morphological features, yield, and its components, which include 17 qualitative and quantitative traits, were recorded during the observation. Among the cucumber landraces, the Shannon–Weaver diversity index analysis revealed the presence of genetic diversity in these landraces. The biggest diversity appeared in the fruit-related characteristics, i.e., stem end fruit shape, bottom end fruit shape, fruit shape, and fruit skin colour at the table and harvest maturity. The descriptive statistics and analysis of variance expressed a wide range of variability for quantitative traits. A broad phenotypic variation was also observed for traits such as yield plant⁻¹ [CV (%) 31.88, ranges 0.96 to 3.11 kg] and fruits plant⁻¹ (CV (%), 28.71, ranges, 2.58 to 9.75). High heritability (broad sense) coupled with a high genetic gain was observed for yield and yield-contributing characteristics, indicating that these characteristics are controlled by additive gene effects, and they are more reliable for effective selection. The phenotypic correlation studies showed that fruit yield plant⁻¹ exhibited a positive and significant correlation with fruits plant⁻¹, fruit length, fruit weight, fruit width, branches plant⁻¹, and plant height. All landraces were grouped into six clusters, and the maximum number of landraces were accommodated in cluster VI (30), followed by cluster V (22), cluster III (22), cluster IV (14), cluster I (13), and cluster II (2). Comparing cluster means with studied traits revealed that cluster III with landraces AC-14, AC-97, AC-471, AC-451, and RAI-209 were more divergent for improving average fruit weight, fruit length, and fruit width. On the other hand, cluster IV with landraces AC-201, TT-161, RAI- 217, RAI-215, and TRMR-103 were more divergent for improving average vine length, internode length, and the number of primary branches plant⁻¹, the number of fruits plant⁻¹, and yield plant⁻¹. According to the MGIDI index, AC-14 (G1), AC-201 (G7), AC-471 (G24), AC-97 (G30), RAI-215 (G68) and TT-161 (G 94) may be considered to be the best parents based on their qualitative and quantitative characteristics for the future breeding programme. Moreover, crossing between the landraces, which were collected from saline and drought areas, in clusters I, V, and VI with those in other clusters could produce suitable cucumber varieties for the climatic changing situation.

The introduction of heavy metals by industries in the aquatic ecosystem is a threatening alarm for living organisms. Bioremediation is an eco-friendly and inexpensive technique as an alternative to chemical methods for wastewater treatment. Wastewater samples were collected from ponds receiving effluents from the Kot Lakhpat Industrial zone, Lahore (Pakistan). Heavy metal-resistant ciliate, Paramecium multimicronucleatum was isolated and characterized with the help of 18SrRNA biomarker. The ciliate showed optimum growth at 25 °C ± 1 °C and pH 7. Growth patterns of P. multimicronucleatum were observed with and without metal stress in wheat grain medium. The minimum inhibitory concentration of cadmium, copper, zinc, and lead was 60, 70, 110, and 160 μg ml⁻¹, respectively. The maximum uptake by Paramecia for Cd, Cu, and Zn was recorded as 90%, 82%, and 93% respectively after 96 h of exposure in each case. While 90% of lead ions were absorbed by Paramecium cells after 48 h of exposure. The order of uptake ability by Paramecium sp. was Zn²⁺ > Cd²⁺ > Cu²⁺ > Pb²⁺. This preliminary study of exploring bioremediation ability of this ciliate would be helpful for investigating it further using advanced molecular techniques.

Exposure to cadmium chloride (CdCl₂) causes an imbalance in the oxidant status of the body by triggering the release of reactive oxygen species (ROS). This study investigated the effect of Rosa damascena (R. damascena) extract on oxidative stress, hepatotoxicity, and the injured cardiac tissue of male rats exposed to CdCl₂. Forty male Wistar albino rats were divided into four groups: the vehicle control (1 mg/kg normal saline), the CdCl₂-treated group (5 mg/kg), the R. damascena extract group (100 mg Kg), and the combination of CdCl₂ and R. damascena extract group. Male rats exposed to CdCl₂ showed multiple significant histopathological changes in the liver and heart, including inflammatory cell infiltration and degenerative alterations. Successive exposure to CdCl₂ elevated the levels of hepatic and cardiac reactive oxygen species (ROS), malondialdehyde (MDA), tumour necrosis factor-alpha) (TNF-α) and interleukin -6 (IL-6) and decreased antioxidant defences. The extracts significantly increased the levels of glutathione, superoxide dismutase (SOD), and catalase (CAT), whereas it dramatically decreased the levels of lipid peroxidation (LPO), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and the mRNA of TNF-α and IL-6. R. damascena administration prevented liver and heart injury; suppressed excessive ROS generation, LPO, and inflammatory responses; and enhanced antioxidant defences. In addition, R. damascena upregulated the mRNA of TNF-α and IL-6 in CdCl₂-administered male rats. In conclusion, R. damascena modulated the oxidative stress and inflammation induced by CdCl₂. The hepatic and cardiac tissue damage and histopathological alterations resulting from the CdCl₂-induced oxidative stress were counteracted by the administration of R. damascena extracts. R. damascena enhanced antioxidant defence enzymes in male rats.

Trichinellosis is a serious food-borne parasitic disease with serious community health effects, mainly causing muscle damage with no recent approved treatment. This study aimed to assess the therapeutic effect of gallic acid (GA) as a potent antioxidant against the encysted phase of Trichinella spiralis in male (BALB/c) mice alone or combined with albendazole (ALB) and to detect their synergistic effects on the histology and ultrastructure of skeletal and cardiac muscles and some biochemical blood analyses. Forty male mice were randomly divided into five groups (8 mice/group). 1^st group: the negative control received only distilled water, 2^nd group: the positive control (infected control group without treatment), 3^rd group: infected group plus treatment with ALB (50 mg Kg^-1 orally), and 4^th group: infected group and then treated with GA (30 mg Kg^-1 orally) and finally 5^th infected group treated with a combination of both ALB and GA. Aspartate and Alanine aminotransferase, Lactate dehydrogenase, alkaline phosphatase, C-reactive protein, Interleukin-4 and Creatine kinase were used as biochemical markers of hepatic and cardiac toxicity and inflammation. Malondialdehyde level, catalase, superoxide dismutase, and glutathione peroxidase were evaluated in heart tissue homogenates beside histological and ultra-structural examination of heart and skeletal muscles beside parasitological analyses. Results showed that the reduction % of Trichinella sp. larvae g^-1 in muscles of the group treated with the combination of GA and ALB showed overall reduction percentages. Oral administration of 30 mg kg¹ of GA led to infection reduction of T. spiralis than ALB treated group. Both administration of ALB beside GA showed the best treatment group that resulted in high infection reduction besides amelioration of both biochemical markers and restoration of histological and ultrastructures to normal state. In conclusion, GA is highly effective against T. spiralis which could be a promising alternative antioxidant drug and the GA effect was higher in the case of combination with ALB. This experiment provides a basis for further exploration of potent activities of other antioxidants against different phases of T. spiralis and the reduction of any health hazards prospectively.