The species from Bulbophyllinae are generally regarded as fly-pollinated: myophilous or sapromyophilous. The section Cirrhopetalum is characterized by umbellate inflorescence and elongated lateral sepals. The aim of the floral anatomical investigation (micromorphology, histochemistry, ultrastructure) in Bulbophyllum wendlandianum (section Cirrhopetalum) was the detection of secretory activity. The appendages of dorsal sepal and petals function as osmophores. The exudation is transported inside vesicles via granulocrine secretion. The cuticle stretches and forms swellings on the entire cell surface. Such swellings of cuticle on the appendages of dorsal sepal and petals were not previously described in Bulbophyllum species. The nectary is located in the central groove on the adaxial lip surface. It comprises epidermal epithelial cells and few subepidermal layers.
The use of elemental metallic powders and in situ alloying in additive manufacturing (AM) is of industrial relevance as it offers the required flexibility to tailor the batch powder composition. This solution has been applied to the AM manufacturing of nickel-titanium (NiTi) shape memory alloy components. In this work, we show that laser powder bed fusion (LPBF) can be used to create a Ni55.7Ti44.3 alloyed component, but that the chemical composition of the build has a large heterogeneity. To solve this problem three different annealing heat treatments were designed, and the resulting porosity, microstructural homogeneity, and phase formation was investigated. The heat treatments were found to improve the alloy’s chemical and phase homogeneity, but the brittle NiTi2 phase was found to be stabilized by the 0.54 wt.% of oxygen present in all fabricated samples. As a consequence, a Ni2Ti4O phase was formed and was confirmed by transmission electron microscopy (TEM) observation. This study showed that pore formation in in situ alloyed NiTi can be controlled via heat treatment. Moreover, we have shown that the two-step heat treatment is a promising method to homogenise the chemical and phase composition of in situ alloyed NiTi powder fabricated by LPBF.