The appropriate increase of planting densities is the key to the obtainment of high-yield maize (Zea mays L.). However, lodging is a major constraint to limit grain yield under increased planting density in present maize production. Effects of population density on stalk lodging and agronomic traits were investigated using two maize cultivars Denghai 618 (DH618, low stalk with low spike height) and Xianyu335 (XY335, high stalk with high spike height). Four levels of density treatment were imposed by 1.5, 6.0, 7.5, and 9.0 × 10⁴ plants ha⁻¹. Results showed that bending strength, rind penetration strength, maximum bending strength, dry weight, and internode diameter of maize were significantly decreased with the increase of planting density. The change range of XY335 with the increase of planting density was significantly larger than that of DH618, showing a high sensitivity to planting density. In addition, the thickness of cortex and vascular bundle sclerenchyma cells was significantly reduced with the increase of planting density. Compared with 1.5 × 10⁴ plants ha⁻¹, the thickness of the above-ground third internode stem cortex was decreased by 9.64%, 12.72%, and 20.77% for DH618, and 19.26%, 30.49%, and 37.45% for XY335 at 6.0, 7.5, and 9.0 × 10⁴ plants ha⁻¹, respectively. The thickness of vascular bundle sclerenchyma cells at 1.5 × 10⁴ plants ha⁻¹ was decreased by 7.75%, 12.44%%, and 17.89% for DH618, 10.18%, 15.21%, and 24.73% for XY335, compared to those at 6.0, 7.5, and 9.0 × 10⁴ plants ha⁻¹, respectively. Visibly, with the increase of planting density, the thickness of cortex and vascular bundle sclerenchyma cells, and the number of vascular bundles were all significantly decreased, resulting in the increase of lodging rate. However, the extent of variation in these parameters for short-plant height hybrid was less than those for high-plant height hybrid, and the yield of short-plant height hybrid was greater than that of high-plant height hybrid, indicating that short-plant height hybrid has better resistance to lodging with higher yield at higher planting density. Therefore, lodging resistance and yield can be improved through selection and breeding strategies that achieving synergistic development of diameter, dry weight per unit, and cortex thickness in maize basal internodes.

Increasing planting density is an effective way to improve maize yield, but high plant populations often cause a lodging problem. This experiment was conducted to investigate the effect of increasing planting density on stalk lodging resistance and grain yield, and to explore the effects on stalk and yield properties of spraying ethephon in densely planted summer maize. The summer maize hybrid, Xundan20 (XD20), was used as experimental material. It was grown by spraying water (CK) or ethephon (E) at BBCH (BASF, Bayer, Ciba-Geigy and Hoechst) 17 under three different planting densities of 60,000 plants ha⁻¹ (L), 75,000 plants ha⁻¹ (M) and 90,000 plants ha⁻¹ (H) in order to explore the possibility of synergistic improvement in stalk lodging resistance and grain yield. The results from this experiment suggested that the gravity center height of densely planted summer maize was significantly increased, the stem diameter, area and number of vascular bundles were significantly decreased and the dry weight per unit internode was significantly decreased, thereby weakening the stalk rind penetration strength and bending performance, resulting in a significant increase in lodging percentage. The ear height was significantly decreased and the SPAD (soil and plant analysis development) and canopy light transmittance were increased after spraying ethephon; then, the internode dry weight per unit length was increased and the stalk rind penetration strength and bending performance were enhanced so as to significantly reduce the lodging percentage and increase the grain yield. The correlation analysis further showed that lodging percentage was significantly negatively correlated with stem diameter, area and number of vascular bundles and stalk bending performance, but there were no strong relationships with grain yield. This suggested that the synergistic improvement in stalk lodging resistance and grain yield was not contradictory. Under the experiment conditions, the effect of spraying ethephon was most significant when the planting density was 90,000 plants ha⁻¹. At the time, the lodging percentage and grain yield were 12.2% and 11,137.5 kg ha⁻¹, which were decreased by 44.6% and increased by 8.0% compared with the control treatment. Scientific chemical regulation could significantly improve the stalk lodging resistance and grain yield of densely planted summer maize.