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The experiment was conducted to evaluate the effects of five rubber seed oil (RSO) levels (0, 1%, 2%, 4%, and 6%) on hens laying performance, egg quality, and yolks fatty acid composition and cholesterol contents. Three hundred and sixty 30-week-old Lohmann Brown laying hens were allotted to 5 groups. The results showed that the egg production was increased in 4% RSO group (P < 0.05), but egg quality parameters and the contents of dry matter, lipid, and protein in yolks were not influenced among treatments (P > 0.05). Yolk cholesterol contents were reduced in RSO supplemental groups (P < 0.05). The concentration of total n-3 PUFA in yolks increased gradually while the ratio of n-6/n-3 decreased gradually with increasing dietary RSO levels (P < 0.001). In conclusion, dietary RSO supplementation increased yolk n-3 PUFA levels, improved yolk color, and reduced yolk cholesterol contents without negative influence on laying performance parameters.
Zhiguo Wen; Yongbao Wu; Zhiguo Qi; Xiumei Li; Fuhuang Li; Xuezhuang Wu; Peilong Yang. Rubber seed oil supplementation enriches n-3 polyunsaturated fatty acids and reduces cholesterol contents of egg yolks in laying hens. Food Chemistry 2019, 301, 125198 .
AMA StyleZhiguo Wen, Yongbao Wu, Zhiguo Qi, Xiumei Li, Fuhuang Li, Xuezhuang Wu, Peilong Yang. Rubber seed oil supplementation enriches n-3 polyunsaturated fatty acids and reduces cholesterol contents of egg yolks in laying hens. Food Chemistry. 2019; 301 ():125198.
Chicago/Turabian StyleZhiguo Wen; Yongbao Wu; Zhiguo Qi; Xiumei Li; Fuhuang Li; Xuezhuang Wu; Peilong Yang. 2019. "Rubber seed oil supplementation enriches n-3 polyunsaturated fatty acids and reduces cholesterol contents of egg yolks in laying hens." Food Chemistry 301, no. : 125198.
In recent years, the fingerprint of high performance liquid chromatography has been extensively applied in the identification and quality control of traditional Chinese medicine. It can be a potential protocol for assessing the authenticity, stability and consistency of traditional Chinese medicine and guaranteeing the expected biological activity. In this paper, a method using high‐performance liquid chromatography to identify and control the quality of the extract of Taraxacum mongolicum Hand.‐Mazz. (TME) was established. With this method, the correlation coefficients of the similarity of 10 batches were ≥ 0.994. The TME displayed steady proliferative effect in Lactobacillus plantarum. In brief, this study successfully built a reliable, simple and efficient method to control and confirm the quality and the stability of biological activity of the TME.
Jing Liu; Xiu‐Mei Li; Dong‐Dong Shi; Zhi‐Guo Wen; Pei‐Long Yang. Effect of quality control on the proliferation of the extract from Taraxacum mongolicum Hand.‐Mazz. in Lactobacillus plantarum. Biomedical Chromatography 2019, 33, e4687 .
AMA StyleJing Liu, Xiu‐Mei Li, Dong‐Dong Shi, Zhi‐Guo Wen, Pei‐Long Yang. Effect of quality control on the proliferation of the extract from Taraxacum mongolicum Hand.‐Mazz. in Lactobacillus plantarum. Biomedical Chromatography. 2019; 33 (12):e4687.
Chicago/Turabian StyleJing Liu; Xiu‐Mei Li; Dong‐Dong Shi; Zhi‐Guo Wen; Pei‐Long Yang. 2019. "Effect of quality control on the proliferation of the extract from Taraxacum mongolicum Hand.‐Mazz. in Lactobacillus plantarum." Biomedical Chromatography 33, no. 12: e4687.
In this work of quercetin's anti-proliferation action on A. flavus, we revealed that quercetin can effectively hamper the proliferation of A. flavus in dose-effect and time-effect relationships. We tested whether quercetin induced apoptosis in A. flavus via various detection methods, such as phosphatidylserine externalization and Hoechst 33342 staining. The results showed that quercetin had no effect on phosphatidylserine externalization and cell nucleus in A. flavus. Simultaneously, quercetin reduced the levels of reactive oxygen species (ROS). For a better understanding of the molecular mechanism of the A. flavus response to quercetin, the RNA-Seq was used to explore the transcriptomic profiles of A. flavus. According to transcriptome sequencing data, quercetin inhibits the proliferation and aflatoxin biosynthesis by regulating the expression of development-related genes and aflatoxin production-related genes. These results will provide some theoretical basis for quercetin as an anti-mildew agent resource.
Xiu-Mei Li; Zhong-Yuan Li; Ya-Dong Wang; Jin-Quan Wang; Pei-Long Yang. Quercetin Inhibits the Proliferation and Aflatoxins Biosynthesis of Aspergillus flavus. Toxins 2019, 11, 154 .
AMA StyleXiu-Mei Li, Zhong-Yuan Li, Ya-Dong Wang, Jin-Quan Wang, Pei-Long Yang. Quercetin Inhibits the Proliferation and Aflatoxins Biosynthesis of Aspergillus flavus. Toxins. 2019; 11 (3):154.
Chicago/Turabian StyleXiu-Mei Li; Zhong-Yuan Li; Ya-Dong Wang; Jin-Quan Wang; Pei-Long Yang. 2019. "Quercetin Inhibits the Proliferation and Aflatoxins Biosynthesis of Aspergillus flavus." Toxins 11, no. 3: 154.
Similarity evaluation of complicated chromatographic profiles is a potential proticol for the identification and quality control of herbal medicinal products to ensure their biological activity. In this work, a high-performance liquid chromatography method was established for controlling the batch quality of the extract from Portulaca oleracea L. By using this method, the coefficients of correlation of the similarity of 10 batches extract of Portulaca oleracea L. were≥0.97. The 10 batch extracts from Portulaca oleracea L. possessed stable antiproliferative activity in Aspergillus flavus. The antiproliferative activity stability is correlated with the quality stability of the extract from Portulaca oleracea L. Therefore, the present study successfully set up a sensitive and efficient method which might be used to guarantee stable biological activity of the extract from Portulaca oleracea L.
Xiu-Mei Li; Jing Liu; Fang-Fang Pan; Pei-Long Yang. Effect of quality control on the antiproliferative activity of the extract from Portulaca oleracea L. in Aspergillus flavus. Biomedical Chromatography 2018, 32, e4354 .
AMA StyleXiu-Mei Li, Jing Liu, Fang-Fang Pan, Pei-Long Yang. Effect of quality control on the antiproliferative activity of the extract from Portulaca oleracea L. in Aspergillus flavus. Biomedical Chromatography. 2018; 32 (12):e4354.
Chicago/Turabian StyleXiu-Mei Li; Jing Liu; Fang-Fang Pan; Pei-Long Yang. 2018. "Effect of quality control on the antiproliferative activity of the extract from Portulaca oleracea L. in Aspergillus flavus." Biomedical Chromatography 32, no. 12: e4354.
Up till now, studies have not been conducted on how the combination of Quercetin (Q), Aconitine (A) and apoptosis induction affects human cervical carcinoma HeLa cells. The result of our findings shows that the combination of Q and A (QA) is capable of synergistically inhibiting the proliferation of HeLa cells in a number of concentrations. QA synergistically inhibits the proliferation of MDR1 gene in the HeLa cells. It is concluded based on our result that QA induces apoptosis and ER stress just as QA-induced ER stress pathway may mediate apoptosis by upregulating mRNA expression levels of eIF2α, ATF4, IRE1, XBP1, ATF6, PERK and CHOP in the HeLa cells. The up-regulating of mRNA expression level of GRP78 and activation of UPR are a molecular basis of QA-induced ER stress.
Xiu-Mei Li; Jing Liu; Fang-Fang Pan; Dong-Dong Shi; Zhi-Guo Wen; Pei-Long Yang. Quercetin and aconitine synergistically induces the human cervical carcinoma HeLa cell apoptosis via endoplasmic reticulum (ER) stress pathway. PLOS ONE 2018, 13, e0191062 .
AMA StyleXiu-Mei Li, Jing Liu, Fang-Fang Pan, Dong-Dong Shi, Zhi-Guo Wen, Pei-Long Yang. Quercetin and aconitine synergistically induces the human cervical carcinoma HeLa cell apoptosis via endoplasmic reticulum (ER) stress pathway. PLOS ONE. 2018; 13 (1):e0191062.
Chicago/Turabian StyleXiu-Mei Li; Jing Liu; Fang-Fang Pan; Dong-Dong Shi; Zhi-Guo Wen; Pei-Long Yang. 2018. "Quercetin and aconitine synergistically induces the human cervical carcinoma HeLa cell apoptosis via endoplasmic reticulum (ER) stress pathway." PLOS ONE 13, no. 1: e0191062.
Sonchus species (Asteraceae) is a class of edible wild plant, with 50 known species widely distributed in Europe, Asia, and Africa. In recent years, researchers worldwide have studied the potential of this species as a crop for humans and animals. Sonchus species also has many pharmacological activities. Studies have been conducted to understand the applications of the genus, and their results indicated the potential utilization of Sonchus species. This review aimed to compile the scientific information in the literature on Sonchus species, including its potential nutrient, medicinal and feed uses, and to identify possible future applications of this plant.
Xiu-Mei Li; Pei-Long Yang. Research progress of Sonchus species. International Journal of Food Properties 2018, 21, 147 -157.
AMA StyleXiu-Mei Li, Pei-Long Yang. Research progress of Sonchus species. International Journal of Food Properties. 2018; 21 (1):147-157.
Chicago/Turabian StyleXiu-Mei Li; Pei-Long Yang. 2018. "Research progress of Sonchus species." International Journal of Food Properties 21, no. 1: 147-157.
Similarity evaluation of complicated chromatographic profiles is a potential instrument for the identification and quality control of herbal medicinal products to ensure the prospective biological activity. The present study was implemented to evaluate the quality of Sonchus brachyotus DC. sampled using high-performance liquid chromatography (HPLC) fingerprinting coupled with a suite of chemometrics methods. By this means, the coefficients of correlation of similarity of 10 batches extract of S. brachyotus DC. were >0.98. The 10 batches extract of Sonchus brachyotus DC. showed stable antioxidant capacity in vitro. The results of total antioxidant capacity were in accordance and completed well for the quality evaluation of Sonchus brachyotus DC. Thus, HPLC fingerprinting coupled with chemometrics techniques provides a greatly flexible and dependable method for the quality assessment of the extract of Sonchus brachyotus DC.
Fang-Fang Pan; Hai-Ying Zhang; Xiu-Mei Li; Pei-Long Yang; Tong-Cun Zhang; Xue-Gang Luo; Wen-Jian Ma. Effect of quality control on the total antioxidant capacity of the extract from Sonchus brachyotus DC. International Journal of Food Properties 2018, 21, 1362 -1370.
AMA StyleFang-Fang Pan, Hai-Ying Zhang, Xiu-Mei Li, Pei-Long Yang, Tong-Cun Zhang, Xue-Gang Luo, Wen-Jian Ma. Effect of quality control on the total antioxidant capacity of the extract from Sonchus brachyotus DC. International Journal of Food Properties. 2018; 21 (1):1362-1370.
Chicago/Turabian StyleFang-Fang Pan; Hai-Ying Zhang; Xiu-Mei Li; Pei-Long Yang; Tong-Cun Zhang; Xue-Gang Luo; Wen-Jian Ma. 2018. "Effect of quality control on the total antioxidant capacity of the extract from Sonchus brachyotus DC." International Journal of Food Properties 21, no. 1: 1362-1370.
Portulaca oleracea L. (P. oleracea) has been used as a Chinese herbal medicine for the treatment of antipyretic, anti-scorbutic, antiseptic, antispasmodic, diuretic, anthelmintic, and urinary disorders. However, pharmacological studies are necessary to provide a scientific basis to substantiate its effectiveness. In this study, the antimicrobial effect and its mechanism of the flavonoids extract from P. oleracea. (POFE) were investigated. The antibacterial effects of POFE were tested on Enterobacter cloacae, Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus, Salmonella enterica and Micrococcus luteus, and the results showed that some bacteria, especially Staphylococcus aureus, were sensitive to POFE. At the tested concentrations, POFE-treated cells displayed various apoptotic markers, such as reactive oxygen species (ROS) accumulation, membrane depolarisation, phosphatidylserine (PS) exposure, DNA fragmentation, and caspase-like protein, in a dose-dependently manner. Based on these data, we concluded that POFE entered the cytoplasm through the membrane pores of Staphylococcus aureus, and subsequently, two parallel reactions took place, namely increase in the level of ROS and DNA fragmentation, which caused cell death.
Yong-Kai Du; Jing Liu; Xiu-Mei Li; Fang-Fang Pan; Zhi-Guo Wen; Tong-Cun Zhang; Pei-Long Yang. Flavonoids extract from Portulaca oleracea L. induce Staphylococcus aureus death by apoptosis-like pathway. International Journal of Food Properties 2017, 20, S534 -S542.
AMA StyleYong-Kai Du, Jing Liu, Xiu-Mei Li, Fang-Fang Pan, Zhi-Guo Wen, Tong-Cun Zhang, Pei-Long Yang. Flavonoids extract from Portulaca oleracea L. induce Staphylococcus aureus death by apoptosis-like pathway. International Journal of Food Properties. 2017; 20 (sup1):S534-S542.
Chicago/Turabian StyleYong-Kai Du; Jing Liu; Xiu-Mei Li; Fang-Fang Pan; Zhi-Guo Wen; Tong-Cun Zhang; Pei-Long Yang. 2017. "Flavonoids extract from Portulaca oleracea L. induce Staphylococcus aureus death by apoptosis-like pathway." International Journal of Food Properties 20, no. sup1: S534-S542.
Xiu-Mei Li; Jing Liu; Yong-Kai Du; Zhi-Guo Wen; Ji-Tong Wang; Pei-Long Yang. Antibacterial activity and mechanism of the ethanol extracts from Sonchus brachyotus DC. International Journal of Food Properties 2017, 20, 2923 -2931.
AMA StyleXiu-Mei Li, Jing Liu, Yong-Kai Du, Zhi-Guo Wen, Ji-Tong Wang, Pei-Long Yang. Antibacterial activity and mechanism of the ethanol extracts from Sonchus brachyotus DC. International Journal of Food Properties. 2017; 20 (12):2923-2931.
Chicago/Turabian StyleXiu-Mei Li; Jing Liu; Yong-Kai Du; Zhi-Guo Wen; Ji-Tong Wang; Pei-Long Yang. 2017. "Antibacterial activity and mechanism of the ethanol extracts from Sonchus brachyotus DC." International Journal of Food Properties 20, no. 12: 2923-2931.
Xiumei Li. Investigation of Naturally Acquired Antibody to Haemophilus influenzae Type b Polysaccharide in China. Journal of Microbiology and Infectious Diseases 2016, 6, 197 -198.
AMA StyleXiumei Li. Investigation of Naturally Acquired Antibody to Haemophilus influenzae Type b Polysaccharide in China. Journal of Microbiology and Infectious Diseases. 2016; 6 (4):197-198.
Chicago/Turabian StyleXiumei Li. 2016. "Investigation of Naturally Acquired Antibody to Haemophilus influenzae Type b Polysaccharide in China." Journal of Microbiology and Infectious Diseases 6, no. 4: 197-198.