Enzymes are nature’s catalyst of choice for the highly selective and efficient coupling of carbohydrates. Enzymatic sugar coupling is a competitive technology for industrial glycosylation reactions, since chemical synthetic routes require extensive use of laborious protection group manipulations and often lack regio- and stereoselectivity. The application of Leloir glycosyltransferases has received considerable attention in recent years and offers excellent control over the reactivity and selectivity of glycosylation reactions with unprotected carbohydrates, paving the way for previously inaccessible synthetic routes. The development of nucleotide recycling cascades has allowed for the efficient production and reuse of nucleotide sugar donors in robust one-pot multi-enzyme glycosylation cascades. In this way, large glycans and glycoconjugates with complex stereochemistry can be constructed. With recent advances, LeLoir glycosyltransferases are close to being applied industrially in multi-enzyme, programmable cascade glycosylations.

The synthesis and basic properties of 2‐ethylhexanol based innovative nonionic surfactants are described in this paper. 2‐Ethylhexanol as an available and relatively inexpensive raw material was used as the hydrophobe source modified by propoxylation and followed by polyethoxylation. As the result, six series of 2‐ethylhexyl alcohol polyalkoxylates (EHP_mE_n) were obtained with three steps of propoxylation, each followed by polyethoxylation and two series only with polyethoxylation (EHE_n). Two different catalysts were used, a dimetalcyanide and KOH. Values of average conversion rates and chemical content of the obtained products (GC, TG and GPC techniques) were compared. The influence of the applied catalyst and polyaddition degree on the homologue distribution, reactant conversion and amount of byproducts is discussed. The basic physicochemical parameters including refractive index, solubility in polar media, foaming properties and wettability were investigated and compared. Furthermore, surface activity parameters, i.e. surface tension (γ_CMC) and critical micelle concentrations were determined. Results are compared to C_12–14 alcohol ethoxylates (LaE_n). Accordingly, it was found that the studied 2‐ethylhexyl alcohol based compounds are effective, low foaming nonionic surfactants.

Clostridioides difficile infections (CDIs) are the most common cause of acquired diseases in hospitalized patients. Effective surface disinfection, focused on the inactivation of the spores of this pathogen, is a decisive factor in reducing the number of nosocomial cases of CDI infections. An efficient disinfection procedure is the result of both the properties of the biocidal agent used and the technology of its implementation as well as a reliable, experimental methodology for assessing the activity of the biocidal active substance based on laboratory models that adequately represent real clinical conditions. This study reviews the state of knowledge regarding the properties and biochemical basis of the action mechanisms of sporicidal substances, with emphasis on chlorine dioxide (ClO₂). Among the analyzed biocides, in addition to ClO₂, active chlorine, hydrogen peroxide, peracetic acid, and glutaraldehyde were characterized. Due to the relatively high sporicidal effectiveness and effective control of bacterial biofilm, as well as safety in a health and environmental context, the use of ClO₂ is an attractive alternative in the control of nosocomial infections of CD etiology. In terms of the methods of assessing the biocidal effectiveness, suspension and carrier standards are discussed.