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University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca (Romania)
University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca (Romania)
Polytechnic University of Valencia
Oscar Vicente obtained his PhD in Sciences in 1983 from the Autonomous University of Madrid. After a postdoc at the Friedich-Miescher Institut in Basel, in 1988 he moved to the Institute of Microbiology and Genetics, University of Vienna, where he established a research group working on plant reproductive biology, and on the characterization of pollen allergens and plant MAP kinases. Oscar Vicente returned to Spain in 1996, to join the faculty of the Polytechnic University of Valencia, where he is at present Professor of Biochemistry and Molecular Biology at the Department of Biotechnology. He has been teaching courses in this area to undergraduate and Master students and supervising a large number of Bachelor, Master and PhD Theses, as well as the work of Erasmus students and visiting scientists. His research interest focuses on the study of plant responses to abiotic stress. Oscar Vicente is Vice-president of the European Biotechnology Thematic Network Association (EBTNA)
Allelopathy is an ecological phenomenon that involves the production and release of biomolecules from different crops, cultivated plants, and bacteria or fungi into the soil rhizosphere and impacts other organisms in the vicinity. Sorghum possesses vital allelopathic characteristics due to which it produces and releases different biomolecules from its root hairs, stems, and grains. Several studies have reported that sorghum acts as an allelopathic crop, decreasing the growth and eco-physiological attributes of surrounding plants and weeds growing simultaneously or subsequently in the field. Sorghum allelopathy has been exploited in the context of green manure, crop rotations, cover crops, and intercropping or mulching, whereas plant aqueous extracts or powder might be an alternate method of weed control. A diverse group of allelochemicals, including benzoic acid, p-hydroxybenzoic acid, vanillic acid, ferulic acid, chlorogenic acid, m-coumaric acid, p-coumaric acid, gallic acid, caffeic acid, p-hydroxibenzaldehyde, dhurrin, sorgoleone, m-hydroxybenzoic acid and protocatechuic acid, have been isolated and identified from different plant tissues of sorghum and root exudates. These allelochemicals, especially sorgoleone, have been investigated in terms of their mode(s) of action, specific activity and selectivity, release in the rhizosphere and uptake and translocation in sensitive species. The present review describes the importance of sorghum allelopathy as an ecological tool in managing weeds, highlighting the most recent advances in the allelochemicals present in sorghum, their modes of action, and their fate in the ecosystem. Further research should focus on the evaluation and selection of sorghum cultivars with high allelopathic potential, so that sorghum allelopathy can be better utilized for weed control and yield enhancement.
M. Iftikhar Hussain; Subhan Danish; Adela M. Sánchez-Moreiras; Óscar Vicente; Khawar Jabran; Usman Khalid Chaudhry; Ferdinando Branca; Manuel J. Reigosa. Unraveling Sorghum Allelopathy in Agriculture: Concepts and Implications. Plants 2021, 10, 1795 .
AMA StyleM. Iftikhar Hussain, Subhan Danish, Adela M. Sánchez-Moreiras, Óscar Vicente, Khawar Jabran, Usman Khalid Chaudhry, Ferdinando Branca, Manuel J. Reigosa. Unraveling Sorghum Allelopathy in Agriculture: Concepts and Implications. Plants. 2021; 10 (9):1795.
Chicago/Turabian StyleM. Iftikhar Hussain; Subhan Danish; Adela M. Sánchez-Moreiras; Óscar Vicente; Khawar Jabran; Usman Khalid Chaudhry; Ferdinando Branca; Manuel J. Reigosa. 2021. "Unraveling Sorghum Allelopathy in Agriculture: Concepts and Implications." Plants 10, no. 9: 1795.
The genus Plantago is particularly interesting for studying the mechanisms of salt tolerance in plants, as it includes both halophytes and glycophytes, as well as species adapted to xeric environments. In this study, the salt stress responses of two halophytes, P. crassifolia and P. coronopus, were compared with those of two glycophytes, P. ovata and P. afra. Plants obtained by seed germination of the four species, collected in different regions of Tunisia, were subjected to increasing salinity treatments for one month under greenhouse conditions. Morphological traits and biochemical parameters, such as ion accumulation and the leaf contents of photosynthetic pigments, osmolytes, oxidative stress markers and antioxidant metabolites, were measured after the treatments. Salt-induced growth inhibition was more pronounced in P. afra, and only plants subjected to the lowest applied NaCl concentration (200 mM) survived until the end of the treatments. The biochemical responses were different in the two groups of plants; the halophytes accumulated higher Na+ and proline concentrations, whereas MDA levels in their leaves decreased, indicating a lower level of oxidative stress. Overall, the results showed that P. coronopus and P. crassifolia are the most tolerant to salt stress, and P. afra is the most susceptible of the four species. Plantago ovata is also quite resistant, apparently by using specific mechanisms of tolerance that are more efficient than in the halophytes, such as a less pronounced inhibition of photosynthesis, the accumulation of higher levels of Cl− ions in the leaves, or the activation of K+ uptake and transport to the aerial part under high salinity conditions.
Hela Ltaeif; Anis Sakhraoui; Sara González-Orenga; Anbu Landa Faz; Monica Boscaiu; Oscar Vicente; Slim Rouz. Responses to Salinity in Four Plantago Species from Tunisia. Plants 2021, 10, 1392 .
AMA StyleHela Ltaeif, Anis Sakhraoui, Sara González-Orenga, Anbu Landa Faz, Monica Boscaiu, Oscar Vicente, Slim Rouz. Responses to Salinity in Four Plantago Species from Tunisia. Plants. 2021; 10 (7):1392.
Chicago/Turabian StyleHela Ltaeif; Anis Sakhraoui; Sara González-Orenga; Anbu Landa Faz; Monica Boscaiu; Oscar Vicente; Slim Rouz. 2021. "Responses to Salinity in Four Plantago Species from Tunisia." Plants 10, no. 7: 1392.
Soil salinity and the indiscriminate use of agrochemicals has significantly reduced the productivity of the ‘Chinampas’ agroecosystem in Mexico City. Crop improvement under these stressful conditions may be achieved by soil bioremediation. In this study, we checked the effects of the organochlorine pesticide endosulfan and bioremediation with Penicillium crustosum or a citric waste on the growth of Phaseolus leptostachyus plants in saline soil from the Chinampas area. Biochemical markers associated with specific stress responses were also determined after one month of growth in the different substrates. Plant growth was stimulated by bioremediation of the soil. Both biostimulants reduced the degree of stress affecting the plants, as shown by the increase in photosynthetic pigments and the reduction of proline, malondialdehyde (MDA), and H2O2 contents, and the activation of antioxidant systems. However, the biostimulants appeared to mitigate oxidative stress through different mechanisms. Endosulfan contamination inhibited seed germination—which was reverted to control values in the presence of the biostimulants—and further decreased plant growth. No clear patterns of variation of biochemical stress markers were observed combining endosulfan and the biostimulants. In any case, bioremediation with P. crustosum and/or citric waste is recommended to improve the germination and growth of P. leptostachyus plants.
Anbu Landa-Faz; Sara González-Orenga; Monica Boscaiu; Refugio Rodríguez-Vázquez; Oscar Vicente. Effect of the Pesticide Endosulfan and Two Different Biostimulants on the Stress Responses of Phaseolus leptostachyus Plants Grown in a Saline Soil. Agronomy 2021, 11, 1208 .
AMA StyleAnbu Landa-Faz, Sara González-Orenga, Monica Boscaiu, Refugio Rodríguez-Vázquez, Oscar Vicente. Effect of the Pesticide Endosulfan and Two Different Biostimulants on the Stress Responses of Phaseolus leptostachyus Plants Grown in a Saline Soil. Agronomy. 2021; 11 (6):1208.
Chicago/Turabian StyleAnbu Landa-Faz; Sara González-Orenga; Monica Boscaiu; Refugio Rodríguez-Vázquez; Oscar Vicente. 2021. "Effect of the Pesticide Endosulfan and Two Different Biostimulants on the Stress Responses of Phaseolus leptostachyus Plants Grown in a Saline Soil." Agronomy 11, no. 6: 1208.
Sugarcane (Saccharum spp.) is one of the most important industrial cash crops, contributing to the world sugar industry and biofuel production. It has been cultivated and improved from prehistoric times through natural selection and conventional breeding and, more recently, using the modern tools of genetic engineering and biotechnology. However, the heterogenicity, complex poly-aneuploid genome and susceptibility of sugarcane to different biotic and abiotic stresses represent impediments that require us to pay greater attention to the improvement of the sugarcane crop. Compared to traditional breeding, recent advances in breeding technologies (molecular marker-assisted breeding, sugarcane transformation, genome-editing and multiple omics technologies) can potentially improve sugarcane, especially against environmental stressors. This article will focus on efficient modern breeding technologies, which provide crucial clues for the engineering of sugarcane cultivars resistant to environmental stresses.
Rubab Shabbir; Talha Javed; Irfan Afzal; Ayman Sabagh; Ahmad Ali; Oscar Vicente; Pinghua Chen. Modern Biotechnologies: Innovative and Sustainable Approaches for the Improvement of Sugarcane Tolerance to Environmental Stresses. Agronomy 2021, 11, 1042 .
AMA StyleRubab Shabbir, Talha Javed, Irfan Afzal, Ayman Sabagh, Ahmad Ali, Oscar Vicente, Pinghua Chen. Modern Biotechnologies: Innovative and Sustainable Approaches for the Improvement of Sugarcane Tolerance to Environmental Stresses. Agronomy. 2021; 11 (6):1042.
Chicago/Turabian StyleRubab Shabbir; Talha Javed; Irfan Afzal; Ayman Sabagh; Ahmad Ali; Oscar Vicente; Pinghua Chen. 2021. "Modern Biotechnologies: Innovative and Sustainable Approaches for the Improvement of Sugarcane Tolerance to Environmental Stresses." Agronomy 11, no. 6: 1042.
Increasing nutrient use efficiency of fertilizers is one of the major challenges to improve crop yields and minimize environmental impacts. This work compared the efficacy of a new ecological polymer-coated urea fertilizer and a slow release urea-based traditional fertilizer. Reductions in the N doses of the polymer-coated fertilizer were tested. A comparative study was first carried out by measuring the different physiological and yield parameters at the micro-scale level, and later-on field experiments were performed. Grain yield in the field was significantly higher (20%) when applying the new controlled-release fertilizer than when using the traditional one at the same dose. A 20% reduction in N content in the new fertilizer gave similar physiological and yield responses compared to the traditional fertilizer. We conclude that this new fertilizer can be used in extensive cropping of maize, guaranteeing at least the same yields than traditional fertilizers, with a reduction on the impact on soil properties and nitrogen losses.
Ricardo Gil-Ortiz; Miguel Naranjo; Antonio Ruiz-Navarro; Marcos Caballero-Molada; Sergio Atares; Carlos García; Oscar Vicente. Agronomic Assessment of a Controlled-Release Polymer-Coated Urea-Based Fertilizer in Maize. Plants 2021, 10, 594 .
AMA StyleRicardo Gil-Ortiz, Miguel Naranjo, Antonio Ruiz-Navarro, Marcos Caballero-Molada, Sergio Atares, Carlos García, Oscar Vicente. Agronomic Assessment of a Controlled-Release Polymer-Coated Urea-Based Fertilizer in Maize. Plants. 2021; 10 (3):594.
Chicago/Turabian StyleRicardo Gil-Ortiz; Miguel Naranjo; Antonio Ruiz-Navarro; Marcos Caballero-Molada; Sergio Atares; Carlos García; Oscar Vicente. 2021. "Agronomic Assessment of a Controlled-Release Polymer-Coated Urea-Based Fertilizer in Maize." Plants 10, no. 3: 594.
Limonium is one of the most interesting and biodiverse genera of halophytes, with many species adapted to saline environments. Limonium species have a promising potential as cultivated minor crops as many have ornamental value, or are already used as medicinal plants. Other species are marketed as gourmet food or can be used for decontamination of polluted soils. Design and implementation of specific breeding programmes are needed to fully realise this potential, based on the vast genetic variation and high stress tolerance of wild species within the genus. Most Limonium species are halophytes, but many are also resistant to drought, especially those from the Mediterranean and other arid regions. Such species constitute attractive models for basic research on the mechanisms of stress tolerance, both constitutive and induced. As typical recretohalopyhtes, with excretive salt glands, Limonium species possess remarkable morpho-anatomical traits. Salt tolerance in this genus relies also on ion accumulation in the leaves, the concomitant use of diverse osmolytes for osmotic adjustment, and the activation of efficient antioxidant systems.
Sara González-Orenga; Marius-Nicusor Grigore; Monica Boscaiu; Oscar Vicente. Constitutive and Induced Salt Tolerance Mechanisms and Potential Uses of Limonium Mill. Species. Agronomy 2021, 11, 413 .
AMA StyleSara González-Orenga, Marius-Nicusor Grigore, Monica Boscaiu, Oscar Vicente. Constitutive and Induced Salt Tolerance Mechanisms and Potential Uses of Limonium Mill. Species. Agronomy. 2021; 11 (3):413.
Chicago/Turabian StyleSara González-Orenga; Marius-Nicusor Grigore; Monica Boscaiu; Oscar Vicente. 2021. "Constitutive and Induced Salt Tolerance Mechanisms and Potential Uses of Limonium Mill. Species." Agronomy 11, no. 3: 413.
Climate change and its detrimental effects on agricultural production, freshwater availability and biodiversity accentuated the need for more stress-tolerant varieties of crops. This requires unraveling the underlying pathways that convey tolerance to abiotic stress in wild relatives of food crops, industrial crops and ornamentals, whose tolerance was not eroded by crop cycles. In this work we try to demonstrate the feasibility of such strategy applying and investigating the effects of saline stress in different species and cultivars of Portulaca. We attempted to unravel the main mechanisms of stress tolerance in this genus and to identify genotypes with higher tolerance, a procedure that could be used as an early detection method for other ornamental and minor crops. To investigate these mechanisms, six-week-old seedlings were subjected to saline stress for 5 weeks with increasing salt concentrations (up to 400 mM NaCl). Several growth parameters and biochemical stress markers were determined in treated and control plants, such as photosynthetic pigments, monovalent ions (Na+, K+ and Cl−), different osmolytes (proline and soluble sugars), oxidative stress markers (malondialdehyde—a by-product of membrane lipid peroxidation—MDA) and non-enzymatic antioxidants (total phenolic compounds and total flavonoids). The applied salt stress inhibited plant growth, degraded photosynthetic pigments, increased concentrations of specific osmolytes in both leaves and roots, but did not induce significant oxidative stress, as demonstrated by only small fluctuations in MDA levels. All Portulaca genotypes analyzed were found to be Na+ and Cl− includers, accumulating high amounts of these ions under saline stress conditions, but P. grandiflora proved to be more salt tolerant, showing only a small reduction under growth stress, an increased flower production and the lowest reduction in K+/Na+ rate in its leaves.
Orsolya Borsai; Mohamad Hassan; Cornel Negrușier; M. Raigón; Monica Boscaiu; Radu Sestraș; Oscar Vicente. Responses to Salt Stress in Portulaca: Insight into Its Tolerance Mechanisms. Plants 2020, 9, 1660 .
AMA StyleOrsolya Borsai, Mohamad Hassan, Cornel Negrușier, M. Raigón, Monica Boscaiu, Radu Sestraș, Oscar Vicente. Responses to Salt Stress in Portulaca: Insight into Its Tolerance Mechanisms. Plants. 2020; 9 (12):1660.
Chicago/Turabian StyleOrsolya Borsai; Mohamad Hassan; Cornel Negrușier; M. Raigón; Monica Boscaiu; Radu Sestraș; Oscar Vicente. 2020. "Responses to Salt Stress in Portulaca: Insight into Its Tolerance Mechanisms." Plants 9, no. 12: 1660.
Pineapple is an economically important tropical fruit crop, but the lack of adequate planting material limits its productivity. A range of micropropagation protocols has been developed over the years to address this shortfall. Still, the final stage of micropropagation, i.e. acclimatisation, remains a challenge as pineapple plantlets grow very slowly. Several studies have been conducted focusing on this phase and attempting to improve plantlet growth and establishment, which requires tools for the non-destructive evaluation of growth during acclimatisation. This report describes the use of semi-automated and automated image analysis to quantify canopy growth of pineapple plantlets, during five months of acclimatisation. The canopy area progressively increased during acclimatisation, particularly after 90 days. Regression analyses were performed to determine the relationships between the automated image analysis and morphological indicators of growth. The mathematical relationships between estimations of the canopy area and the fresh and dry weights of intact plantlets, middle-aged leaves (D leaves) and roots showed determination coefficients (R2) between 0.84 and 0.92. We propose an appropriate tool for the simple, objective and non-destructive evaluation of pineapple plantlets growth, which can be generally applied for plant phenotyping, to reduce costs and develop streamlined pipelines for the assessment of plant growth.
Guillermo Soto; Gustavo Lorente; Jessica Mendoza; Evelio Dany Báez; Carlos Manuel Lorenzo; Romelio Rodríguez; Elliosha Hajari; Oscar Vicente; José Carlos Lorenzo. Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy. The EuroBiotech Journal 2020, 4, 223 -229.
AMA StyleGuillermo Soto, Gustavo Lorente, Jessica Mendoza, Evelio Dany Báez, Carlos Manuel Lorenzo, Romelio Rodríguez, Elliosha Hajari, Oscar Vicente, José Carlos Lorenzo. Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy. The EuroBiotech Journal. 2020; 4 (4):223-229.
Chicago/Turabian StyleGuillermo Soto; Gustavo Lorente; Jessica Mendoza; Evelio Dany Báez; Carlos Manuel Lorenzo; Romelio Rodríguez; Elliosha Hajari; Oscar Vicente; José Carlos Lorenzo. 2020. "Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy." The EuroBiotech Journal 4, no. 4: 223-229.
Thalictrum maritimum is an endangered, endemic species in East Spain, growing in areas of relatively low salinity in littoral salt marshes. A regression of its populations and the number of individuals has been registered in the last decade. This study aimed at establishing the causes of this reduction using a multidisciplinary approach, including climatic, ecological, physiological and biochemical analyses. The climatic data indicated that there was a direct negative correlation between increased drought, especially during autumn, and the number of individuals censused in the area of study. The susceptibility of this species to water deficit was confirmed by the analysis of growth parameters upon a water deficit treatment applied under controlled greenhouse conditions, with the plants withstanding only 23 days of complete absence of irrigation. On the other hand, increased salinity does not seem to be a risk factor for this species, which behaves as a halophyte, tolerating in controlled treatments salinities much higher than those registered in its natural habitat. The most relevant mechanisms of salt tolerance in T. maritimum appear to be based on the control of ion transport, by (i) the active transport of toxic ions to the aerial parts of the plants at high external salinity—where they are presumably stored in the leaf vacuoles to avoid their deleterious effects in the cytosol, (ii) the maintenance of K+ concentrations in belowground and aboveground organs, despite the increase of Na+ levels, and (iii) the salt-induced accumulation of Ca2+, particularly in stems and leaves. This study provides useful information for the management of the conservation plans of this rare and endangered species.
Sara González-Orenga; Calin Trif; Mͣ Pilar Donat-Torres; Josep V. Llinares; Francisco Collado; P. Pablo Ferrer-Gallego; Emilio Laguna; Monica Boscaiu; Oscar Vicente. Responses to Increased Salinity and Severe Drought in the Eastern Iberian Endemic Species Thalictrum maritimum (Ranunculaceae), Threatened by Climate Change. Plants 2020, 9, 1251 .
AMA StyleSara González-Orenga, Calin Trif, Mͣ Pilar Donat-Torres, Josep V. Llinares, Francisco Collado, P. Pablo Ferrer-Gallego, Emilio Laguna, Monica Boscaiu, Oscar Vicente. Responses to Increased Salinity and Severe Drought in the Eastern Iberian Endemic Species Thalictrum maritimum (Ranunculaceae), Threatened by Climate Change. Plants. 2020; 9 (10):1251.
Chicago/Turabian StyleSara González-Orenga; Calin Trif; Mͣ Pilar Donat-Torres; Josep V. Llinares; Francisco Collado; P. Pablo Ferrer-Gallego; Emilio Laguna; Monica Boscaiu; Oscar Vicente. 2020. "Responses to Increased Salinity and Severe Drought in the Eastern Iberian Endemic Species Thalictrum maritimum (Ranunculaceae), Threatened by Climate Change." Plants 9, no. 10: 1251.
Fertilizer-use efficiency is one of the most critical concerns in rice cultivation to reduce N losses, increase yields, and improve crop management. The effects of a new polymeric-coated controlled-release fertilizer (CRF) were compared to those of other slow-release and traditional fertilizers in a microscale experiment, which was carried out in cuvettes under partly controlled ambient conditions, and a large-scale field experiment. To evaluate the fertilizer’s efficiency, nitrogen and water-use efficiency were calculated using the measurement of different photosynthetic and crop yield parameters. Improved responses regarding some of the analyzed physiological and growth parameters were observed for those plants fertilized with the new CRF. In the microscale experiment, significantly increased yields (ca. 35%) were produced in the plants treated with CRF as compared to traditional fertilizer. These results were in accordance with ca. 24% significant increased levels of N in leaves of CRF-treated plants, besides increased P, Fe, Mn, and cytokinin contents. At the field scale, similar yields were obtained with the slow-release or traditional fertilizers and CRF at a 20% reduced N dose. The new controlled-release fertilizer is a urea-based fertilizer coated with lignosulfonates, which is cheaply produced from the waste of pulp and wood industries, containing humic acids as biostimulants. In conclusion, CRF is recommended to facilitate rice crop management and to reduce contamination, as it can be formulated with lower N doses and because it is ecological manufacturing.
Ricardo Gil-Ortiz; Miguel Ángel Naranjo; Antonio Ruiz-Navarro; Sergio Atares; Carlos García; Lincoln Zotarelli; Alberto San Bautista; Oscar Vicente. Enhanced Agronomic Efficiency Using a New Controlled-Released, Polymeric-Coated Nitrogen Fertilizer in Rice. Plants 2020, 9, 1183 .
AMA StyleRicardo Gil-Ortiz, Miguel Ángel Naranjo, Antonio Ruiz-Navarro, Sergio Atares, Carlos García, Lincoln Zotarelli, Alberto San Bautista, Oscar Vicente. Enhanced Agronomic Efficiency Using a New Controlled-Released, Polymeric-Coated Nitrogen Fertilizer in Rice. Plants. 2020; 9 (9):1183.
Chicago/Turabian StyleRicardo Gil-Ortiz; Miguel Ángel Naranjo; Antonio Ruiz-Navarro; Sergio Atares; Carlos García; Lincoln Zotarelli; Alberto San Bautista; Oscar Vicente. 2020. "Enhanced Agronomic Efficiency Using a New Controlled-Released, Polymeric-Coated Nitrogen Fertilizer in Rice." Plants 9, no. 9: 1183.
This study investigated the physiological and biochemical responses to salinity stress of Solanum melongena and its wild relative, Solanum torvum, commonly used as eggplant rootstock. Young plants of both species were watered during 25 days with NaCl aqueous solutions at the following four final concentrations: 0 (for the controls), 100, 200, and 300 mM. Plant growth parameters, photosynthetic pigments content, monovalent ion concentrations in roots and leaves, leaf levels of osmolytes (proline and total soluble sugars), oxidative stress markers (MDA and H2O2), non-enzymatic antioxidants (total phenolic compounds and total flavonoids), and enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione reductase) were determined after the stress treatments. Salt-induced growth reduction was more significant in S. melongena than in S. torvum, especially at high salt concentrations, indicating a (slightly) higher salt tolerance of the wild species. The mechanisms of tolerance of S. torvum were partly based on the active transport of toxic ions to the leaves at high external salinity and, presumably, a better capacity to store them in the vacuoles, as well as on the accumulation of proline to higher concentrations than in the cultivated eggplant. MDA and H2O2 contents did not vary in response to the salt treatments in S. torvum. However, in S. melongena, MDA content increased by 78% when 300 mM NaCl was applied. No activation of antioxidant mechanisms, accumulation of antioxidant compounds, or increase in the specific activity of antioxidant enzymes in any of the studied species was induced by salinity. The relatively high salt tolerance of S. torvum supports its use as rootstock for eggplant cultivation in salinized soils and as a possible source of salt-tolerance genes for the genetic improvement of cultivated eggplant.
Marco Brenes; Jason Pérez; Sara González-Orenga; Andrea Solana; Monica Boscaiu; Jaime Prohens; Mariola Plazas; Ana Fita; Oscar Vicente. Comparative Studies on the Physiological and Biochemical Responses to Salt Stress of Eggplant (Solanum melongena) and Its Rootstock S. torvum. Agriculture 2020, 10, 328 .
AMA StyleMarco Brenes, Jason Pérez, Sara González-Orenga, Andrea Solana, Monica Boscaiu, Jaime Prohens, Mariola Plazas, Ana Fita, Oscar Vicente. Comparative Studies on the Physiological and Biochemical Responses to Salt Stress of Eggplant (Solanum melongena) and Its Rootstock S. torvum. Agriculture. 2020; 10 (8):328.
Chicago/Turabian StyleMarco Brenes; Jason Pérez; Sara González-Orenga; Andrea Solana; Monica Boscaiu; Jaime Prohens; Mariola Plazas; Ana Fita; Oscar Vicente. 2020. "Comparative Studies on the Physiological and Biochemical Responses to Salt Stress of Eggplant (Solanum melongena) and Its Rootstock S. torvum." Agriculture 10, no. 8: 328.
The selection of stress-resistant cultivars, to be used in breeding programmes aimed at enhancing the drought and salt tolerance of our major crops, is an urgent need for agriculture in a climate change scenario. In the present study, the responses to water deficit and salt stress treatments, regarding growth inhibition and leaf proline (Pro) contents, were analysed in 47 Phaseolus vulgaris genotypes of different origins. A two-way analysis of variance (ANOVA), Pearson moment correlations and principal component analyses (PCAs) were performed on all measured traits, to assess the general responses to stress of the investigated genotypes. For most analysed growth variables and Pro, the effects of cultivar, treatment and their interactions were highly significant (p < 0.001); the root morphological traits, stem diameter and the number of leaves were mostly due to uncontrolled variation, whereas the variation of fresh weight and water content of stems and leaves was clearly induced by stress. Under our experimental conditions, the average effects of salt stress on plant growth were relatively weaker than those of water deficit. In both cases, however, growth inhibition was mostly reflected in the stress-induced reduction of fresh weight and water contents of stems and leaves. Pro, on the other hand, was the only variable showing a negative correlation with all growth parameters, but particularly with those of stems and leaves mentioned above, as indicated by the Pearson correlation coefficients and the loading plots of the PCAs. Therefore, in common beans, higher stress-induced accumulation of Pro is unequivocally associated with a stronger inhibition of growth; that is, with a higher sensitivity to stress of the corresponding cultivar. We propose the use of Pro as a suitable biochemical marker for simple, rapid, large-scale screenings of bean genotypes, to exclude the most sensitive, those accumulating higher Pro concentrations in response to water or salt stress treatments.
Sugenith Arteaga; Lourdes Yabor; María José Díez; Jaime Prohens; Monica Boscaiu; Oscar Vicente. The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes. Agronomy 2020, 10, 817 .
AMA StyleSugenith Arteaga, Lourdes Yabor, María José Díez, Jaime Prohens, Monica Boscaiu, Oscar Vicente. The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes. Agronomy. 2020; 10 (6):817.
Chicago/Turabian StyleSugenith Arteaga; Lourdes Yabor; María José Díez; Jaime Prohens; Monica Boscaiu; Oscar Vicente. 2020. "The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes." Agronomy 10, no. 6: 817.
Assessment of the diversity of reproductive traits and genetic variation is of great relevance to the conservation of genetic resources and management of silver fir (Abies alba) populations. We have evaluated reproductive characteristics associated with female cones and seed morphology, as well as seed germination after subjecting seeds to five storage methods, in nine Romanian populations of A. alba. The genetic diversity of the populations was assessed with 12 polymorphic simple sequence repeat (SSR) markers. We detected significant differences between populations for all reproductive traits and considerable differences in seed germination and storage methods; seed storage in wet sand was the method resulting in the highest germination in all populations. Genomic SSRs (gSSRs) were more informative on average than expressed sequence tag SSRs (EST-SSRs) in the populations studied. The nine populations were genetically diverse, with an average number of alleles (N) per SSR locus between 3.50 and 4.83. The observed heterozygosity (Ho) in the nine populations was always lower than the expected heterozygosity (He), which resulted in values of the inbreeding coefficient (Fis) between 0.261 and 0.709. Genetic distances between populations ranged between 0.077 and 0.410. The cluster analysis based on genetic distances did not group accessions according to their geographical proximity, and despite a positive trend, the correlation between geographic and genetic distances was non-significant. The results of an analysis of molecular variance (AMOVA) revealed that only 9.1% of the total molecular variance is attributable to differences between populations. This low degree of genetic differentiation between populations is confirmed by the intermingling of individuals of different populations in a principal coordinate analysis (PCoA). We found evidence of a positive relationship between He and germination, as well as a negative one between Fis and germination, suggesting that populations with low diversity and high consanguinity may have a reduced fitness and long-term viability. The results are relevant for the conservation and management of local genetic resources and populations, as well as for reforestation programmes of silver fir.
Irina Maria Todea Morar; Stephanie Rensen; Santiago Vilanova; Monica Boscaiu; Liviu Holonec; Adriana F. Sestras; Oscar Vicente; Jaime Prohens; Radu E. Sestras; Mariola Plazas. Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations. Sustainability 2020, 12, 4199 .
AMA StyleIrina Maria Todea Morar, Stephanie Rensen, Santiago Vilanova, Monica Boscaiu, Liviu Holonec, Adriana F. Sestras, Oscar Vicente, Jaime Prohens, Radu E. Sestras, Mariola Plazas. Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations. Sustainability. 2020; 12 (10):4199.
Chicago/Turabian StyleIrina Maria Todea Morar; Stephanie Rensen; Santiago Vilanova; Monica Boscaiu; Liviu Holonec; Adriana F. Sestras; Oscar Vicente; Jaime Prohens; Radu E. Sestras; Mariola Plazas. 2020. "Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations." Sustainability 12, no. 10: 4199.
Global warming is not only affecting arid and semi-arid regions but also becoming a threat to agriculture in Central and Eastern European countries. The present study analyzes the responses to drought and salinity of two varieties of Lavandula angustifolia cultivated in Romania. Lavender seedlings were subjected to one month of salt stress (100, 200, and 300 mM NaCl) and water deficit (complete withholding of irrigation) treatments. To assess the effects of stress on the plants, several growth parameters and biochemical stress markers (photosynthetic pigments, mono and divalent ions, and different osmolytes) were determined in control and stressed plants after the treatments. Both stress conditions significantly inhibited the growth of the two varieties, but all plants survived the treatments, indicating a relative stress tolerance of the two varieties. The most relevant mechanisms of salt tolerance are based on the maintenance of foliar K+ levels and the accumulation of Ca2+ and proline as a functional osmolyte in parallel with increasing external salinities. Under water stress, significant increases of Na+ and K+ concentrations were detected in roots, indicating a possible role of these cations in osmotic adjustment, limiting root dehydration. No significant differences were found when comparing the stress tolerance and stress responses of the two selected lavender varieties.
Zsolt Szekely-Varga; Sara González-Orenga; Maria Cantor; Denisa Jucan; Monica Boscaiu; Oscar Vicente. Effects of Drought and Salinity on Two Commercial Varieties of Lavandula angustifolia Mill. Plants 2020, 9, 637 .
AMA StyleZsolt Szekely-Varga, Sara González-Orenga, Maria Cantor, Denisa Jucan, Monica Boscaiu, Oscar Vicente. Effects of Drought and Salinity on Two Commercial Varieties of Lavandula angustifolia Mill. Plants. 2020; 9 (5):637.
Chicago/Turabian StyleZsolt Szekely-Varga; Sara González-Orenga; Maria Cantor; Denisa Jucan; Monica Boscaiu; Oscar Vicente. 2020. "Effects of Drought and Salinity on Two Commercial Varieties of Lavandula angustifolia Mill." Plants 9, no. 5: 637.
Eggplant (Solanum melongena) has been described as moderately sensitive to salinity. We characterised the responses to salt stress of eggplant and S. insanum, its putative wild ancestor. Young plants of two accessions of both species were watered for 25 days with an irrigation solution containing NaCl at concentrations of 0 (control), 50, 100, 200, and 300 mM. Plant growth, photosynthetic activity, concentrations of photosynthetic pigments, K+, Na+, and Cl− ions, proline, total soluble sugars, malondialdehyde, total phenolics, and total flavonoids, as well as superoxide dismutase, catalase, and glutathione reductase specific activities, were quantified. Salt stress-induced reduction of growth was greater in S. melongena than in S. insanum. The photosynthetic activity decreased in both species, except for substomatal CO2 concentration (Ci) in S. insanum, although the photosynthetic pigments were not degraded in the presence of NaCl. The levels of Na+ and Cl− increased in roots and leaves with increasing NaCl doses, but leaf K+ concentrations were maintained, indicating a relative stress tolerance in the two accessions, which also did not seem to suffer a remarkable degree of salt-induced oxidative stress. Our results suggest that the higher salt tolerance of S. insanum mostly lies in its ability to accumulate higher concentrations of proline and, to a lesser extent, Na+ and Cl−. The results obtained indicate that S. insanum is a good candidate for improving salt tolerance in eggplant through breeding and introgression programmes.
Marco Brenes; Andrea Solana; Monica Boscaiu; Ana Fita; Oscar Vicente; Ángeles Calatayud; Jaime Prohens; Mariola Plazas. Physiological and Biochemical Responses to Salt Stress in Cultivated Eggplant (Solanum melongena L.) and in S. insanum L., a Close Wild Relative. Agronomy 2020, 10, 651 .
AMA StyleMarco Brenes, Andrea Solana, Monica Boscaiu, Ana Fita, Oscar Vicente, Ángeles Calatayud, Jaime Prohens, Mariola Plazas. Physiological and Biochemical Responses to Salt Stress in Cultivated Eggplant (Solanum melongena L.) and in S. insanum L., a Close Wild Relative. Agronomy. 2020; 10 (5):651.
Chicago/Turabian StyleMarco Brenes; Andrea Solana; Monica Boscaiu; Ana Fita; Oscar Vicente; Ángeles Calatayud; Jaime Prohens; Mariola Plazas. 2020. "Physiological and Biochemical Responses to Salt Stress in Cultivated Eggplant (Solanum melongena L.) and in S. insanum L., a Close Wild Relative." Agronomy 10, no. 5: 651.
Forest ecosystems are frequently exposed to abiotic stress, which adversely affects their growth, resistance and survival. For silver fir (Abies alba), the physiological and biochemical responses to water and salt stress have not been extensively studied. Responses of one-year-old seedlings to a 30-day water stress (withholding irrigation) or salt stress (100, 200 and 300 mM NaCl) treatments were analysed by determining stress-induced changes in growth parameters and different biochemical markers: accumulation of ions, different osmolytes and malondialdehyde (MDA, an oxidative stress biomarker), in the seedlings, and activation of enzymatic and non-enzymatic antioxidant systems. Both salt and water stress caused growth inhibition. The results obtained indicated that the most relevant responses to drought are based on the accumulation of soluble carbohydrates as osmolytes/osmoprotectants. Responses to high salinity, on the other hand, include the active transport of Na+, Cl− and Ca2+ to the needles, the maintenance of relatively high K+/Na+ ratios and the accumulation of proline and soluble sugars for osmotic balance. Interestingly, relatively high Na+ concentrations were measured in the needles of A. alba seedlings at low external salinity, suggesting that Na+ can contribute to osmotic adjustment as a ‘cheap’ osmoticum, and its accumulation may represent a constitutive mechanism of defence against stress. These responses appear to be efficient enough to avoid the generation of high levels of oxidative stress, in agreement with the small increase in MDA contents and the relatively weak activation of the tested antioxidant systems.
Irina Maria Todea (Morar); Sara González-Orenga; Monica Boscaiu; Mariola Plazas; Adriana F. Sestras; Jaime Prohens; Oscar Vicente; Radu E. Sestras. Responses to Water Deficit and Salt Stress in Silver Fir (Abies alba Mill.) Seedlings. Forests 2020, 11, 395 .
AMA StyleIrina Maria Todea (Morar), Sara González-Orenga, Monica Boscaiu, Mariola Plazas, Adriana F. Sestras, Jaime Prohens, Oscar Vicente, Radu E. Sestras. Responses to Water Deficit and Salt Stress in Silver Fir (Abies alba Mill.) Seedlings. Forests. 2020; 11 (4):395.
Chicago/Turabian StyleIrina Maria Todea (Morar); Sara González-Orenga; Monica Boscaiu; Mariola Plazas; Adriana F. Sestras; Jaime Prohens; Oscar Vicente; Radu E. Sestras. 2020. "Responses to Water Deficit and Salt Stress in Silver Fir (Abies alba Mill.) Seedlings." Forests 11, no. 4: 395.
Knowing the mechanisms that operate under water stress in commercial crops, particularly those that can affect productivity, such as phenolic or cell wall metabolism, is becoming increasingly important in a scenario of global climate change. However, our understanding of how to analyse statistically the relationships between these commonly used biochemical markers of water stress and growth in crops like pineapple, needs to be improved. In the present work, we have addressed the question of whether polynomial regression analysis can be used to describe the influence of selected plant metabolites (chlorophylls, carotenoids, phenolics and aldehydes) on shoot biomass, in response to a mannitol-induced water stress in temporary immersion bioreactors (TIBs). Polynomial regression analysis has been applied to investigate plant stress responses in many species but is very seldom used in in vitro screening studies. Here, the relationship between biochemical indicators (x; independent variable) and shoot growth (y; dependent variable) has been characterised, with y modelled as an nth degree polynomial in x. This statistical approach accommodated for the non-linear (curvilinear) relationships between variables, and the results showed that shoot biomass was negatively, and significantly correlated with soluble phenolics, cell wall-linked phenolics and other aldehydes (characterised by “High” R2 values).
Daviel Gómez; Doris Escalante; Elliosha Hajari; Oscar Vicente; Sershen; José C. Lorenzo. Assessing the effects of in vitro imposed water stress on pineapple growth in relation to biochemical stress indicators using polynomial regression analysis. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2020, 48, 162 -170.
AMA StyleDaviel Gómez, Doris Escalante, Elliosha Hajari, Oscar Vicente, Sershen, José C. Lorenzo. Assessing the effects of in vitro imposed water stress on pineapple growth in relation to biochemical stress indicators using polynomial regression analysis. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2020; 48 (1):162-170.
Chicago/Turabian StyleDaviel Gómez; Doris Escalante; Elliosha Hajari; Oscar Vicente; Sershen; José C. Lorenzo. 2020. "Assessing the effects of in vitro imposed water stress on pineapple growth in relation to biochemical stress indicators using polynomial regression analysis." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 48, no. 1: 162-170.
Presently, there is a growing interest in developing new controlled-release fertilizers based on ecological raw materials. The present study aims to compare the efficacy of two new ureic-based controlled-release fertilizers formulated with water-soluble polymeric coatings enriched with humic acids or seaweed extracts. To this end, an experimental approach was designed under controlled greenhouse conditions by carrying out its subsequent field scaling. Different physiological parameters and crop yield were measured by comparing the new fertilizers with another non polymeric-coated fertilizer, ammonium nitrate, and an untreated ‘Control’. As a result, on the microscale the fertilizer enriched with humic acids favored a better global response in the photosynthetic parameters and nutritional status of wheat plants. A significant 1.2-fold increase in grain weight yield and grain number was obtained with the humic acid polymeric fertilizer versus that enriched with seaweed extracts; and also, in average, higher in respect to the uncoated one. At the field level, similar results were confirmed by lowering N doses by 20% when applying the humic acid polymeric-coated produce compared to ammonium nitrate. Our results showed that the new humic acid polymeric fertilizer facilitated crop management and reduced the environmental impact generated by N losses, which are usually produced by traditional fertilizers.
Ricardo Gil-Ortiz; Miguel Ángel Naranjo; Antonio Ruiz-Navarro; Marcos Caballero-Molada; Sergio Atares; Carlos García; Oscar Vicente. New Eco-Friendly Polymeric-Coated Urea Fertilizers Enhanced Crop Yield in Wheat. Agronomy 2020, 10, 438 .
AMA StyleRicardo Gil-Ortiz, Miguel Ángel Naranjo, Antonio Ruiz-Navarro, Marcos Caballero-Molada, Sergio Atares, Carlos García, Oscar Vicente. New Eco-Friendly Polymeric-Coated Urea Fertilizers Enhanced Crop Yield in Wheat. Agronomy. 2020; 10 (3):438.
Chicago/Turabian StyleRicardo Gil-Ortiz; Miguel Ángel Naranjo; Antonio Ruiz-Navarro; Marcos Caballero-Molada; Sergio Atares; Carlos García; Oscar Vicente. 2020. "New Eco-Friendly Polymeric-Coated Urea Fertilizers Enhanced Crop Yield in Wheat." Agronomy 10, no. 3: 438.
Morphological and biochemical traits of four halophytes of the genus Limonium were analysed in plants sampled from salt marshes in SE Spain. This work aimed to explore the mechanism(s) behind the adaptation of these species to stressful habitats, with particular emphasis on responses to drought. Plants of each species together with soil samples were collected in summer, which is the most stressful season in the Mediterranean. Soil parameters and plant morphological traits were determined, and the levels of several biochemical stress markers in plants were measured using spectrophotometric assays. A multivariate analysis was performed to correlate soil and plant data. Morphological characteristics regarding the underground system topology and several biochemical traits (higher foliar Ca2+, sucrose and glucose, and lower proline, glycine-betaine and fructose) clearly separate L. santapolense individuals from plants of the other three species. Drought tolerance of L. santapolense in the field is mostly dependent on morphological adaptations: when growing in an arid location, plants of this species develop long taproots that can extract water from the deep, moist layers of the soil.
Sara González-Orenga; Josep V. Llinares; Mohamad Al Hassan; Ana Fita; Francisco Collado; Purificación Lisón; Oscar Vicente; Monica Boscaiu. Physiological and morphological characterisation of Limonium species in their natural habitats: Insights into their abiotic stress responses. Plant and Soil 2020, 449, 267 -284.
AMA StyleSara González-Orenga, Josep V. Llinares, Mohamad Al Hassan, Ana Fita, Francisco Collado, Purificación Lisón, Oscar Vicente, Monica Boscaiu. Physiological and morphological characterisation of Limonium species in their natural habitats: Insights into their abiotic stress responses. Plant and Soil. 2020; 449 (1-2):267-284.
Chicago/Turabian StyleSara González-Orenga; Josep V. Llinares; Mohamad Al Hassan; Ana Fita; Francisco Collado; Purificación Lisón; Oscar Vicente; Monica Boscaiu. 2020. "Physiological and morphological characterisation of Limonium species in their natural habitats: Insights into their abiotic stress responses." Plant and Soil 449, no. 1-2: 267-284.
Ariel Villalobos; Lelurlis Nápoles; José Ramón Mendoza; Doris Escalante; Julia Martínez; Oscar Concepción; Byron E. Zevallos; Marcos Edel Martínez-Montero; Inaudis Cejas; Florent Engelmann; Oscar Vicente; José Carlos Lorenzo. Exposure of pineapple shoot tips to liquid nitrogen and cryostorage do not affect the histological status of regenerated plantlets. Romanian Biotechnological Letters 2019, 24, 1061 -1066.
AMA StyleAriel Villalobos, Lelurlis Nápoles, José Ramón Mendoza, Doris Escalante, Julia Martínez, Oscar Concepción, Byron E. Zevallos, Marcos Edel Martínez-Montero, Inaudis Cejas, Florent Engelmann, Oscar Vicente, José Carlos Lorenzo. Exposure of pineapple shoot tips to liquid nitrogen and cryostorage do not affect the histological status of regenerated plantlets. Romanian Biotechnological Letters. 2019; 24 (6):1061-1066.
Chicago/Turabian StyleAriel Villalobos; Lelurlis Nápoles; José Ramón Mendoza; Doris Escalante; Julia Martínez; Oscar Concepción; Byron E. Zevallos; Marcos Edel Martínez-Montero; Inaudis Cejas; Florent Engelmann; Oscar Vicente; José Carlos Lorenzo. 2019. "Exposure of pineapple shoot tips to liquid nitrogen and cryostorage do not affect the histological status of regenerated plantlets." Romanian Biotechnological Letters 24, no. 6: 1061-1066.