Lyophyllum decastes, also known as Luronggu in China, is a culinary edible and medicinal mushroom that was widely cultivated in China in recent years. In the present study, the complete high-quality genome of two mating compatible L. decastes strain was sequenced. The L. decastes LRG-d1-1 genome consists of 47.7 Mb in 15 contigs with a contig N90 of 2.08 Mb and 14,499 predicted gene models. Phylogenetic analysis revealed that L. decastes exhibits a close evolutionary relationship to the Termitomyces and Hypsizygus genus and was diverged from H. marmoreus ~ 45.53 Mya ago. Mating A loci of L. decastes compose of five and four HD genes in two monokaryotic strains, respectively. Mating B loci compose of five STE genes in both two monokaryotic strains. To accelerate the cross-breeding process, we designed four pairs of specific primers and successfully detected both mating types in L. decastes. As a wood-rotting mushroom, a total of 541 genes accounting for 577 CAZymes were identified in the genome of L. decastes. Proteomic analysis revealed that 1,071 proteins including 182 CAZymes and 258 secreted enzymes were identified from four groups (PDB, PDB + bran, PDB + cotton hull, and PDB + sawdust). Two laccases and a quinone reductase were strongly overproduced in lignin-rich cultures, and the laccases were among the top-3 secreted proteins, suggesting an important role in the synergistic decomposition of lignin. These results revealed the robustness of the lignocellulose degradation capacity of L. decastes. This is the first study to provide insights into the evolution and lignocellulose degradation of L. decastes.

Morel mushroom (Morchella spp.) is a rare edible fungus with high nutritional and medicinal value. In China they are cultivated in sandy soils in greenhouses and production of fresh mushrooms reach...

Selenium is a crucial trace element that necessitates exogenous supplementation and plays an essential role in human health, but its facilitation requires the conversion from inorganic to bioavailable organic forms. Selenium-enriched edible fungi provide an effective strategy for selenium fortification. Their easy cultivation, rapid growth, and excellent conversion capabilities make them ideal candidates for achieving selenium enrichment goals. This article reviews various methods for producing selenium-enriched products and highlights the benefits and functional properties of these fungi. It summarizes the mechanisms underlying selenium absorption and transformation within fungal biomass while considering influencing factors, such as environmental conditions, types of edible fungi, and sources of selenium. Furthermore, this article offers developmental recommendations to address current industrial challenges, providing theoretical references to foster healthy and sustainable advancements in this field.