The biostimulant segment is becoming increasingly important worldwide. One of the reasons for this is that fewer plant protection products are placed on the market in the European Union, and environmental sustainability also plays an important role in their use. Biostimulants are often used in several horticultural sectors, including ornamentals, to strengthen plants, achieve commercial standards, produce quality goods, increase plant vitality, and aid harvesting. This paper presents the latest results of the use of biostimulants in horticulture, with special emphasis on ornamental plant production. The legal regulation of biostimulants and their regulatory mechanisms are described in detail in the review. The main groups of biostimulants are also discussed. The response of plants to abiotic stress, in particular physiological, anatomical, and genetic changes, with regard to the application of biostimulants is also detailed. Focus is given to the areas of ornamental crop production, such as sexual and asexual propagation, cultivation, and harvesting, where biostimulants are used.
Plant growth-promoting bacteria (PGPB) and other living organisms can help with the challenges of modern agriculture. PGPB offer ever-expanding possibilities for science and commerce, and the scientific results have been very advanced in recent years. In our current work, we collected the scientific results of recent years and the opinions of experts on the subject. Opinions and results on soil–plant relations, as well as the importance of PGPB and the latest related experiences, are important topics of our review work, which highlights the scientific results of the last 3–4 years. Overall, it can be concluded from all these observations that the bacteria that promote plant development are becoming more and more important in agriculture almost all over the world, thus, promoting more sustainable and environmentally conscious agricultural production and avoiding the use of artificial fertilizers and chemicals. Since many mechanisms of action, namely biochemical and operational processes, are still under investigation, a new emerging scientific direction is expected in the coming years with regard to PGPB, microbial, and other plant growth-stimulating substances, in which omics and microbial modulation also play a leading role.
There is no practical and at the same time objective colour system available for describing cultivated roses (Rosa L. cultivars). For this reason, a new colour classification system was developed which is colorimetrically balanced and appropriate for algorithmic colour identification; however, it is also suitable for field-work. The system is based on the following colorimetric criteria: (A) Each colour category is characterised by a measured petal colour in the CIE L*a*b* standard as the centroid of the category. (B) The CIEDE2000 colour differences between the adjacent centroid colours are limited (5 < ΔE00 < 7). (C) The maximal colour difference between the measured colours in a category is also limited (to 12.12 ΔE00). (D) A measured petal colour can only be classified into an existing category if the colour difference from the centroid colour of the given category is less than 5.81 ΔE00, otherwise a new category is required. (E) A category is only considered non-redundant if it has at least one measured petal colour that cannot be classified elsewhere. (F) The classification of the petal colours is based on the least colour difference from the centroid colours. As a result, 133 colour categories were required for describing all the 8139 petal colours of the rose cultivars of the Budatétény Rose Garden (Hungary). Each colour category has the following parameters: standardised colour name, the colorimetric parameters of the centroid, grouping, RHS colour chart coding, and reference cultivars, which are described in the article.
Glyphosate is still the subject of much debate, as several studies report its effects on the environment. Sunflower (GK Milia CL) was set up as an experimental plant and treated with glyphosate concentrations of 500 ppm and 1000 ppm in two treatments. Glyphosate was found to be absorbed from the soil into the plant organism through the roots, which was also detectable in the leaf and root. Glyphosate was also significantly detected in the plant 5 weeks after treatment and in plants that did not receive glyphosate treatment directly, so it could be taken up through the soil. Based on the morphological results, treatment with higher concentrations (1000 ppm) of glyphosate increased the dried mass and resulted in shorter, thicker roots. Histological results also showed that basal and transporter tissue distortions were observed in the glyphosate-treated plants compared to the control group. Cells were distorted with increasing concentration, vacuoles formed, and the cell wall was weakened in both the leaf-treated and inter-row-treated groups. In the future, it will be worth exploring alternative agricultural technologies that can reduce the risk of glyphosate while increasing economic outcomes. This may make the use of glyphosate more environmentally conscious.
In this study, we examined over 200-year-old Ginkgo biloba L. specimens under different environmental conditions. The overall aim was to explore which factors influence their vitality and general fitness in urban environments and thus their ability to tolerate stressful habitats. In order to determine this, we used a number of different methods, including histological examinations (stomatal density and size) and physiological measurements (peroxidase enzyme activity), as well as assessing the air pollution tolerance index (APTI). The investigation of the genetic relationships between individuals was performed using flow cytometry and miRNA marker methods. The genetic tests revealed that all individuals are diploid, whereas the lus-miR168 and lus-miR408 markers indicated a kinship relation between them. These results show that the effect of different habitat characteristics can be detected through morphological and physiological responses, thus indicating relatively higher stress values for all studied individuals. A significant correlation can be found between the level of adaptability and the relatedness of the examined individuals. These results suggest that Ginkgo biloba L. is well adapted to an environment with increased stress factors and therefore suitable for use in urban areas.
Several recent studies have shown that glyphosate and its metabolite, aminomethylphosphonic acid (AMPA), resist rapid degradation and, therefore, can accumulate in plants. Continuing our previous research, we aimed to investigate the effect of indirectly spraying glyphosate on leaves and soil on non-target plants in the case of Helianthus annuus L. The plants were treated with glyphosate in their 5–6 leaf stages, the effects of which were assessed two weeks later from a morphological and histological point of view, as an evaluation of the residues of glyphosate and its metabolite, AMPA. They had an effect on both treated groups. In the case of the morphological parameters (plant height, number of leaves, and fresh and dried root and green mass), the data of the treated plants were statistically lower than in the case of the control group. The epidermis and the transport tissue system were damaged, and tissue death was observed in plants exposed to glyphosate. Both compounds were detected in all plant parts (roots, stems, lower leaves, and upper leaves), well above the limit of detection (0.025 mg/kg) and limit of quantitation 0.075 mg/kg showing a statistical difference with the control plants. This proved that glyphosate is incorporated into the plant organism even when applied indirectly.