We present a dielectric model and its parameters for Chinese hamster ovary (CHO) cells based on a double-shell structure which includes the cell membrane, cytoplasm, nuclear envelope, and nucleoplasm. Employing a dielectrophoresis (DEP) based technique and a microfluidic system, the DEP response of many single CHO cells is measured and the spectrum of the Clausius-Mossotti factor is obtained. The dielectric parameters of the model are then extracted by curve-fitting to the measured spectral data. Using this approach over the 0.6–10 MHz frequency range, we report the values for CHO cells' membrane permittivity, membrane thickness, cytoplasm conductivity, nuclear envelope permittivity, and nucleoplasm conductivity. The size of the cell and its nuclei are obtained using optical techniques.

We present a microfluidic device for in-flow dielectric characterization of single biological cells. The dielectric spectrum is obtained by measuring the multiple-frequency dielectrophoresis (DEP) response of individual cells as they travel over an array of sensing and actuating electrodes. The DEP induced translation of each cell is detected by measuring the differential impedance of the array using a microwave interferometer, which is capable of sub-attofarad sensitivity, and is coupled to the sensing electrodes. The DEP response of a cell at multiple frequencies in the beta-dispersion region is chosen to discern particular cell dielectric properties as it travels along the array-such as cytoplasm conductivity and membrane capacitance. The Clausius-Mossotti factor of the cell is determined from the measured response signal in conjunction with numerical simulation of its trajectory. The approach is validated through measuring polystyrene microspheres. The DEP response of Chinese hamster ovary cells using two simultaneous frequencies is demonstrated.

Dry food commodities like grains and pulses can be stored safely for several years under controlled storage conditions. The equilibrium moisture content of the packaged grain is one of the most important parameters required to be monitored and controlled for extended safe storage. This paper presents a single element dual-polarized sensing tag based on an annular slot antenna operating at two closely spaced resonant frequencies for radar cross-section-based monitoring of relative humidity in hermetically packaged food commodities. One of the resonant frequencies is functionalized for sensing while the other acts as a built-in reference, mitigating the effects of environmental loading. A polyvinyl alcohol coated interdigitated capacitor, integrated onto the antenna is used as a capacitive transducer for the sensing element. Laboratory scale measurements were carried out using the saturated salt solution method. Results show that the proposed sensor can be used for monitoring a wide range of humidity conditions.

There is currently a need to monitor food products while preserving quality and safety during long-term storage. In this article, a compact low-cost quasi-chipless sensor for monitoring the quality of high-value food items, such as milk and meat products, is presented. The sensor utilizes a dual-band dual-polarized annular ring antenna with an integrated harmonic generator and sensor to receive, modulate, and retransmit the interrogator signal. The resonant frequency of the receiving mode of the antenna is sensitized to the parameter being sensed using a varactor—pH electrode-based transduction scheme. The received signal is doubled using a diode frequency doubler circuit to minimize the clutter from the environment before retransmission. One application of the sensor for monitoring pH is presented. The sensor was shown to be able to successfully monitor the milk souring process.