Capturing high spatial resolution imagery is becoming a standard operation in many agricultural applications. The increased capacity for image capture necessitates corresponding advances in analysis algorithms. This study introduces automated raster geoprocessing methods to automatically extract strawberry (Fragaria × ananassa) canopy size metrics using raster image analysis and utilize the extracted metrics in statistical modeling of strawberry dry weight. Automated canopy delineation and canopy size metrics extraction models were developed and implemented using ArcMap software v 10.7 and made available by the authors. The workflows were demonstrated using high spatial resolution (1 mm resolution) orthoimages and digital surface models (2 mm) of 34 strawberry plots (each containing 17 different plant genotypes) planted on raised beds. The images were captured on a weekly basis throughout the strawberry growing season (16 weeks) between early November and late February. The results of extracting four canopy size metrics (area, volume, average height, and height standard deviation) using automatically delineated and visually interpreted canopies were compared. The trends observed in the differences between canopy metrics extracted using the automatically delineated and visually interpreted canopies showed no significant differences. The R² values of the models were 0.77 and 0.76 for the two datasets and the leave-one-out (LOO) cross validation root mean square error (RMSE) of the two models were 9.2 g and 9.4 g, respectively. The results show the feasibility of using automated methods for canopy delineation and canopy metric extraction to support plant phenotyping applications.

The image-based modeling and simulation of plant growth have numerous and diverse applications. In this study, we used image-based and manual field measurements to develop and validate a methodology to simulate strawberry (Fragaria × ananassa Duch.) plant canopies throughout the Florida strawberry growing season. The simulated plants were used to create a synthetic image using radiative transfer modeling. Observed canopy properties were incorporated into an L-system simulator, and a series of strawberry canopies corresponding to specific weekly observation dates were created. The simulated canopies were compared visually with actual plant images and quantitatively with in-situ leaf area throughout the strawberry season. A simple regression model with L-system-derived and in-situ total leaf areas had an $Adj R^2$ value of 0.78. The L-system simulated canopies were used to derive information needed for image simulation, such as leaf area and leaf angle distribution. Spectral and plant canopy information were used to create synthetic high spatial resolution multispectral images using the Discrete Anisotropic Radiative Transfer (DART) software. Vegetation spectral indices were extracted from the simulated image and used to develop multiple regression models of in-situ biophysical parameters (leaf area and dry biomass), achieving $Adj R^2$ values of 0.63 and 0.50, respectively. The Normalized Difference Vegetation Index (NDVI) and the Red Edge Simple Ratio (SRre) vegetation indices, which utilize the red, red edge, and near infrared bands of the spectrum, were identified as statistically significant variables (p < 0.10). This study showed that both geometric (canopy seize metrics) and spectral variables were successful in modeling in-situ biomass and leaf area. Combining the geometric and spectral variables, however, only slightly improved the prediction model. These results show the feasibility of simulating strawberry canopies and images with inherent geometrical, topological, and spectral properties of real strawberry plants. The simulated canopies and images can be used in applications beyond creating realistic computer graphics for quantitative applications requiring the depiction of vegetation biological processes, such as stress modeling and remote sensing mission planning.