The effective control of the sample concentration within droplets is essential in a broad range of assays in chemistry and biochemistry. Here we provide an electrical method for producing batches of aqueous droplets with various chemical concentrations by exploiting fixed-potential induced-charge electroosmosis (ICEO) flow around a bipolar electrode. By applying an AC electric signal to the bipolar electrode and changing the zeta potential on it, the bipolar electrode acts as a gate electrode for generating asymmetric ICEO flow. The ICEO flow induced transverse vortexes interact with two parallel laminar streams with different chemical compositions. Controlled mixing of the aqueous solutions can be achieved by adjusting the shape and size of the asymmetric vortexes via altering the electric signal applied to the gate electrode. The mixed streams are split at a bifurcation, and one of the streams with a desired controlled concentration is pumped into a flow-focusing geometry to generate droplets with adjustable chemical concentrations. The in-droplet concentration increases in the range of 0.412–1.404 mM, as the applied voltage increases in the range of 0–70 mV at 15 kHz. This approach offers a promising method for on-chip control of chemical concentrations within droplets without labor-intensive dilutions while minimizing the sample consumption, showing great potential for next generation droplet-based applications.

A versatile and elegant method for 3D rotation of cells is proposed for the first time by exploiting a bipolar electrode array. ROT and DEP are further used for cell manipulation and detection of inherent cellular traits.

The rotation of cells is of significant importance in various applications including bioimaging, biophysical analysis and microsurgery. Current methods usually require complicated fabrication processes. Herein, we proposed an induced charged electroosmosis (ICEO) based on a chip manipulation method for rotating cells. Under an AC electric field, symmetric ICEO flow microvortexes formed above the electrode surface can be used to trap and rotate cells. We have discussed the impact of ICEO and dielectrophoresis (DEP) under the experimental conditions. The capabilities of our method have been tested by investigating the precise rotation of yeast cells and K562 cells in a controllable manner. By adjusting the position of cells, the rotation direction can be changed based on the asymmetric ICEO microvortexes via applying a gate voltage to the gate electrode. Additionally, by applying a pulsed signal instead of a continuous signal, we can also precisely and flexibly rotate cells in a stepwise way. Our ICEO-based rotational manipulation method is an easy to use, biocompatible and low-cost technique, allowing rotation regardless of optical, magnetic or acoustic properties of the sample.