Due to their low cost, facile fabrication, and high-power conversion efficiency (PCE), dye-sensitized solar cells (DSSCs) have attracted much attention. Ruthenium (Ru) complex dyes and organic solvent-based electrolytes are typically used in high-efficiency DSSCs. However, Ru dyes are expensive and require a complex synthesis process. Organic solvents are toxic, environmentally hazardous, and explosive, and can cause leakage problems due to their low surface tension. This review summarizes and discusses previous works to replace them with natural dyes and water-based electrolytes to fabricate low-cost, safe, biocompatible, and environmentally friendly DSSCs. Although the performance of “eco-friendly DSSCs” remains less than 1%, continuous efforts to improve the PCE can accelerate the development of more practical devices, such as designing novel redox couples and photosensitizers, interfacial engineering of photoanodes and electrolytes, and biomimetic approaches inspired by natural systems.