The chromosomal characteristics, locations and variations of the C-band positive heterochromatin and telomeric DNA sequences were studied in the European grayling karyotype (Thymallus thymallus, Salmonidae) using conventional C-banding, endonucleases digestion banding, silver nitrate (AgNO₃), chromomycin A₃ and 4′,6-diamidino-2-phenylindole staining techniques as well as fluorescence in situ hybridization (FISH) and primed in situ labelling. Original data on the chromosomal distribution of segments resistant to AluI restriction endonuclease and identification of the C-banded heterochromatin presented here have been used to characterize the grayling karyotype polymorphism. Structural and length polymorphism of the chromosome 21 showing a conspicuous heterochromatin block adjacent to the centromere seems to be the result of the deletion and inversion. Two pairs of nuclear organizer regions (NOR)-bearing chromosomes were found to be polymorphic in size and displaying several distinct forms. FISH with telomeric peptide nucleic acid probe enabled recognition of the conservative telomeric DNA sequences. The karyotype of the thymallid fish is thought to experienced numerous pericentric inversions and internal telomeric sites (ITSs) observed at the pericentromeric regions of the six European grayling metacentric chromosomes are likely relics of the these rearrangements. None of the ITS sites matched either chromosome 21 or NOR bearing chromosomes.

Lakes provide different ecosystem services, including those related to tourism and recreation. Sustainable development principles should be respected in lake tourism planning. The aim of this study was to assess the impact of tourism on the lakeshore zone in a typical post-glacial Lakeland in Northern Poland (Central Europe). An explanatory analysis of the distribution of individual spatial factor values was performed using the SHapley Additive exPlanations algorithm (SHAP). In a first step, the aim was to select a Machine Learning model for modelling based on Shapley values. The greater or lesser influence of a given factor on the tourism function was measured for individual lakes. The final results of ensemble modelling and SHAP were obtained by averaging the results of five random repetitions of the execution of these models. The impact of tourism on the lakeshore zone can be much more accurately determined using an indirect method, by analysing the tourism and recreational infrastructure constantly present there. The values of the indices proposed in the study provide indirect information on the number of tourists using the tourist and recreational facilities and are a measure of the impact of tourism on the lakeshore zone. The developed methodology can be applied to the majority of post-glacial lakes in Europe and other regions of the world in order to monitor the threats resulting from shore zone exploitation. Such studies can be an appropriate tool for management and planning by the relevant authorities.

Cytogenetic analysis of brook trout performed with molecular and conventional methods led to identification of interstitial telomeric sites on one or two subtelocentric chromosomes within the same pair. Morphology and specific patterns of these chromosomes using fluorochromes associated with A/T- or G/C-rich DNA proved that these chromosomes are not sex related. The chromomycin-positive region was located on the short arms of the ITS bearing chromosome pair and flanked by telomeric sequences, suggesting that this part of the chromosome had been translocated from another one. Our observations confirm that GC-rich regions are highly mobile genetic structures, and led to ITS formation on brook trout chromosomes.