Air ventilation rate plays a relevant role in maintaining adequate indoor air quality (IAQ) conditions in public buildings. In general, high ventilation rates ensure good indoor air quality but entail relevant energy consumption. Considering the necessity of balancing IAQ and energy consumption, a correlation between the number of occupants obtained from analysis of CO₂ concentration variation is presented as a general element for controlling the operation of heating ventilation and air cooling (HVAC) systems. The specific CO₂ exhalation rate is estimated using experimental data in some real conditions in university classrooms. A method for the definition of optimal values of air exchange rate is defined, highlighting that the obtained values are much lower than those defined in current technical standards with possibilities of relevant reduction of the total energy consumption.