This paper compares experimental results on the stabilization of clayey dredged material (DM) using quicklime (QL), hydrated lime (HL), portland cement (PC), and Class F fly ash (FA). The DM was a clay, high-plasticity soil, dredged from the US Army Corps of Engineers Port Arthur (USACEPA) facility along the Neches River located in Orange and Jefferson Counties, Texas. The soil consists of a large proportion of clay (66%), has high moisture limits (plasticity index: $\mathrm{PI}=52$ and liquid limit: $\mathrm{LL}=80$ ), and is characterized as a fat clay with silt. Physical properties of the untreated DM, such as unconfined compressive strength (UCS), consistency limits, and compaction behavior, were determined. Stabilizing reagents were mixed in various percentages by dry weight of DM. Results indicate that 12% QL, 6% HL, 6% PC, and 7.5% Class F FA by dry soil weight must be used to meet a minimum benchmark criterion of a PI value below 35, which was established based on Texas Department of Transportation (TxDOT) specifications. Standard compaction tests were carried out in accordance common standards with ASTM D698 to determine the optimum moisture content (OMC) for all mixtures. Stabilized mixtures were prepared at the corresponding OMC, and unconfined compressive tests were run according to common standards. Tests results were compared with the relevant literature, and a linear regression model was developed to predict the PI reduction as a function of initial PI, LL, and stabilizer type and content with good accuracy ($R^2=0.86$ ). Overall, tests showed that stabilization of clayey DM using lime products, PC, or FA significantly improved the DM physical properties, which could then potentially be used as a local fill construction material that is cost-effective and environmentally friendly and reduces the overall use of cement products.

The tightly coupled nature of the electrophilic alkylation reaction sequence catalysed by 24-SMT (sterol C-24-methyltransferase) of land plants and algae can be distinguished by the formation of cationic intermediates that yield phyla-specific product profiles. C-24-methylation of the cycloartenol substrate by the recombinant Glycine max (soybean) 24-SMT proceeds to a single product 24(28)-methylenecycloartanol, whereas the 24-SMT from green algae converts cycloartenol into two products cyclolaudenol [∆(25(27))-olefin] and 24(28)-methylenecycloartanol [(∆24(28))-olefin]. Substrate analogues that differed in the steric-electronic features at either end of the molecule, 26-homocycloartenol or 3β-fluorolanostadiene, were converted by G. max SMT into a single 24(28)-methylene product. Alternatively, incubation of the allylic 26-fluoro cyclosteroid with G. max SMT afforded a bound intermediate that converted in favour of the ∆(25(27))-olefin product via the cyclolaudenol cation formed initially during the C-24-methylation reaction. A portion of the 26-fluorocycloartenol substrate was also intercepted by the enzyme and the corresponding hydrolysis product identified by GC-MS as 26-fluoro-25-hydroxy-24-methylcycloartanol. Finally, the 26-fluorocycloartenols are competitive inhibitors for the methylation of cycloartenol and 26-monofluorocycloartenol generated timedependent inactivation kinetics exhibiting a kinact value of 0.12 min(-1). The ability of soybean 24-SMT to generate a 25-hydroxy alkylated sterol and fluorinated ∆(25(27))-olefins is consistent with our hypothesis that (i) achieving the cyclolaudenyl cation intermediate by electrophilic alkylation of cycloartenol is significant to the overall reaction rate, and (ii) the evolution of variant sterol C-24-methylation patterns is driven by competing reaction channels that have switched in algae from formation of primarily ∆(25(27)) products that convert into ergosterol to, in land plants, formation of ∆(24(28)) products that convert into sitosterol.

To maintain adequate depth of commercial waterways, large quantities of earthen material are dredged and stored on undeveloped placement areas adjacent to the waterway. As dredge placement areas become overwhelmed, an environmental and financial sustainable solution for the reuse of dredged soil is prioritized. In this study, locally dredged material from the Sabine-Neches Waterway was used to explore the potential of dredged material in the production of compressed stabilized earth bricks (CSEBs) for small-scale structures in the region. CSEB mixture designs were developed containing fly ash (FA), Portland cement (PC), hydrated lime (HL), water (W), dredged material (DM), and natural and synthetic fibers. Optimized mixtures designs reached the recommended compressive strength of over 1200 psi. Results showed that that the addition of fibers reduced the compressive and flexural strength of the bricks, with a maximum compressive strength of 1394 psi with a corresponding flexural strength of 381 psi being obtained with fiberless dredge bricks. Multiple coating systems were also tested to increase the resistance of the bricks to weathering and erosion. Results showed that the use of coatings reduced water absorption and increased the bricks resistance to erosion, making them more adept in regions commonly subjected to flooding and heavy wind-driven rains.

Zinc Oxide (ZnO) nanoparticles are suspected to produce toxic effects toward mammalian cells; however, discrepancies in the extent of this effect have been reported between different cell lines. Simultaneously, high levels of ultraviolet (UV-C) radiation can have carcinogenic effects. The mechanism of this effect is also not well understood. Due to similarities in phenotype morphology after cell exposure to ZnO nanoparticles and UV-C irradiation, we emit the hypothesis that the toxicity of both these factors is related to damage of cellular membranes and affect their sterol content. Wild-type Chinese Hamster Ovary (CHO-K1) cells were exposed to ZnO nanoparticles or UV-C radiation. The amount of absorbed ZnO was determined by UV-visible spectroscopy and the changes in sterol profiles were evaluated by gas chromatography. Cell viability after both treatments was determined by microscopy. Comparing morphology results suggested similarities in toxicology events induced by ZnO nanoparticles and UV exposure. UV-C exposure for 360 min disrupts the sterol metabolic pathway by increasing the concentration of cholesterol by 21.6-fold. This increase in cholesterol production supports the hypothesis that UV irradiation has direct consequences in initiating sterol modifications in the cell membrane.