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De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this process, and genes involved in their signaling cascades act as master regulators of the different phases of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration are often interpreted as a consequence of these hormones simply being present in the regeneration media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon source, plays a signaling role and has been recently shown to interact with CK and auxin and to affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the regeneration media and their interaction with sucrose present in the media to their complex signaling outputs that mediate shoot regeneration.
Martin Raspor; Václav Motyka; Abdul Kaleri; Slavica Ninković; Ljiljana Tubić; Aleksandar Cingel; Tatjana Ćosić. Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. International Journal of Molecular Sciences 2021, 22, 8554 .
AMA StyleMartin Raspor, Václav Motyka, Abdul Kaleri, Slavica Ninković, Ljiljana Tubić, Aleksandar Cingel, Tatjana Ćosić. Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. International Journal of Molecular Sciences. 2021; 22 (16):8554.
Chicago/Turabian StyleMartin Raspor; Václav Motyka; Abdul Kaleri; Slavica Ninković; Ljiljana Tubić; Aleksandar Cingel; Tatjana Ćosić. 2021. "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs." International Journal of Molecular Sciences 22, no. 16: 8554.
Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
Sofija Stupar; Milan Dragićević; Vele Tešević; Jovana Stanković-Jeremić; Vuk Maksimović; Tatjana Ćosić; Nina Devrnja; Ljiljana Tubić; Aleksandar Cingel; Branka Vinterhalter; Slavica Ninković; Jelena Savić. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. Plants 2021, 10, 172 .
AMA StyleSofija Stupar, Milan Dragićević, Vele Tešević, Jovana Stanković-Jeremić, Vuk Maksimović, Tatjana Ćosić, Nina Devrnja, Ljiljana Tubić, Aleksandar Cingel, Branka Vinterhalter, Slavica Ninković, Jelena Savić. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. Plants. 2021; 10 (1):172.
Chicago/Turabian StyleSofija Stupar; Milan Dragićević; Vele Tešević; Jovana Stanković-Jeremić; Vuk Maksimović; Tatjana Ćosić; Nina Devrnja; Ljiljana Tubić; Aleksandar Cingel; Branka Vinterhalter; Slavica Ninković; Jelena Savić. 2021. "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle." Plants 10, no. 1: 172.
Aquaporins comprise a large group of transmembrane proteins responsible for water transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins, nothing is known about this protein family in Impatiens walleriana, a commercially important horticultural plant, which is sensitive to drought stress. In the present study, attention is given to the molecular characterization of aquaporins in I. walleriana and their expression during drought stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein. Drought stress and recovery treatment affected the aquaporins expression in I. walleriana leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively. Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins expanded knowledge about plant aquaporins, while its expression during drought and recovery contributed to I. walleriana drought tolerance mechanisms and re-acclimation.
Marija Đurić; Angelina Subotić; Ljiljana Prokić; Milana Trifunović-Momčilov; Aleksandar Cingel; Milan Dragićević; Ana Simonović; Snežana Milošević. Molecular Characterization and Expression of Four Aquaporin Genes in Impatiens walleriana During Drought Stress and Recovery. Plants 2021, 10, 154 .
AMA StyleMarija Đurić, Angelina Subotić, Ljiljana Prokić, Milana Trifunović-Momčilov, Aleksandar Cingel, Milan Dragićević, Ana Simonović, Snežana Milošević. Molecular Characterization and Expression of Four Aquaporin Genes in Impatiens walleriana During Drought Stress and Recovery. Plants. 2021; 10 (1):154.
Chicago/Turabian StyleMarija Đurić; Angelina Subotić; Ljiljana Prokić; Milana Trifunović-Momčilov; Aleksandar Cingel; Milan Dragićević; Ana Simonović; Snežana Milošević. 2021. "Molecular Characterization and Expression of Four Aquaporin Genes in Impatiens walleriana During Drought Stress and Recovery." Plants 10, no. 1: 154.
This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.
Marija Đurić; Angelina Subotić; Ljiljana Prokić; Milana Trifunović-Momčilov; Aleksandar Cingel; Milorad Vujičić; Snežana Milošević. Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. Plants 2020, 9, 1559 .
AMA StyleMarija Đurić, Angelina Subotić, Ljiljana Prokić, Milana Trifunović-Momčilov, Aleksandar Cingel, Milorad Vujičić, Snežana Milošević. Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. Plants. 2020; 9 (11):1559.
Chicago/Turabian StyleMarija Đurić; Angelina Subotić; Ljiljana Prokić; Milana Trifunović-Momčilov; Aleksandar Cingel; Milorad Vujičić; Snežana Milošević. 2020. "Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro." Plants 9, no. 11: 1559.
Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors—ancymidol (A) and paclobutrazol (P)—the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb’s dormancy and its sprouting.
Marija Marković; Milana Trifunović Momčilov; Branka Uzelac; Aleksandar Cingel; Snežana Milošević; Slađana Jevremović; Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content. Plants 2020, 9, 1449 .
AMA StyleMarija Marković, Milana Trifunović Momčilov, Branka Uzelac, Aleksandar Cingel, Snežana Milošević, Slađana Jevremović, Angelina Subotić. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content. Plants. 2020; 9 (11):1449.
Chicago/Turabian StyleMarija Marković; Milana Trifunović Momčilov; Branka Uzelac; Aleksandar Cingel; Snežana Milošević; Slađana Jevremović; Angelina Subotić. 2020. "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content." Plants 9, no. 11: 1449.
A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.
Martin Raspor; Václav Motyka; Slavica Ninković; Petre I. Dobrev; Jiří Malbeck; Tatjana Ćosić; Aleksandar Cingel; Jelena Savić; Vojin Tadić; Ivana Č. Dragićević. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics. Scientific Reports 2020, 10, 1 -13.
AMA StyleMartin Raspor, Václav Motyka, Slavica Ninković, Petre I. Dobrev, Jiří Malbeck, Tatjana Ćosić, Aleksandar Cingel, Jelena Savić, Vojin Tadić, Ivana Č. Dragićević. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics. Scientific Reports. 2020; 10 (1):1-13.
Chicago/Turabian StyleMartin Raspor; Václav Motyka; Slavica Ninković; Petre I. Dobrev; Jiří Malbeck; Tatjana Ćosić; Aleksandar Cingel; Jelena Savić; Vojin Tadić; Ivana Č. Dragićević. 2020. "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics." Scientific Reports 10, no. 1: 1-13.
Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
Martin Raspor; Václav Motyka; Slavica Ninković; Jiří Malbeck; Petre I. Dobrev; Snežana Zdravković-Korać; Ana Simonović; Tatjana Ćosić; Aleksandar Cingel; Jelena Savić; Lenka Zahajská; Vojin Tadić; Ivana Č. Dragićević. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. Journal of Plant Growth Regulation 2020, 40, 37 -47.
AMA StyleMartin Raspor, Václav Motyka, Slavica Ninković, Jiří Malbeck, Petre I. Dobrev, Snežana Zdravković-Korać, Ana Simonović, Tatjana Ćosić, Aleksandar Cingel, Jelena Savić, Lenka Zahajská, Vojin Tadić, Ivana Č. Dragićević. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. Journal of Plant Growth Regulation. 2020; 40 (1):37-47.
Chicago/Turabian StyleMartin Raspor; Václav Motyka; Slavica Ninković; Jiří Malbeck; Petre I. Dobrev; Snežana Zdravković-Korać; Ana Simonović; Tatjana Ćosić; Aleksandar Cingel; Jelena Savić; Lenka Zahajská; Vojin Tadić; Ivana Č. Dragićević. 2020. "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization." Journal of Plant Growth Regulation 40, no. 1: 37-47.
Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators (PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18?2?C on half-strength MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA), gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a distinctive manner, with a variety of morphological traits being altered in comparison to matching controls.
Tatjana Cosic; Jelena Savic; Martin Raspor; Aleksandar Cingel; Nabil Ghalawnji; Branka Vinterhalter; Slavica Ninkovic. Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. Archives of Biological Sciences 2020, 72, 349 -357.
AMA StyleTatjana Cosic, Jelena Savic, Martin Raspor, Aleksandar Cingel, Nabil Ghalawnji, Branka Vinterhalter, Slavica Ninkovic. Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. Archives of Biological Sciences. 2020; 72 (3):349-357.
Chicago/Turabian StyleTatjana Cosic; Jelena Savic; Martin Raspor; Aleksandar Cingel; Nabil Ghalawnji; Branka Vinterhalter; Slavica Ninkovic. 2020. "Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro." Archives of Biological Sciences 72, no. 3: 349-357.
The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na–FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.
Milena Trajković; Dragana Antonic; Aleksandar Cingel; Nabil Ghalawenji; Angelina Subotić; Slađana Jevremović. Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta. 3 Biotech 2019, 9, 17 .
AMA StyleMilena Trajković, Dragana Antonic, Aleksandar Cingel, Nabil Ghalawenji, Angelina Subotić, Slađana Jevremović. Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta. 3 Biotech. 2019; 9 (1):17.
Chicago/Turabian StyleMilena Trajković; Dragana Antonic; Aleksandar Cingel; Nabil Ghalawenji; Angelina Subotić; Slađana Jevremović. 2019. "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta." 3 Biotech 9, no. 1: 17.
In this study we investigated physiological parameters of stress (enzymatic and non-enzymatic) in lettuce (Lactuca sativa L.) and its hairy roots induced by water solution of phenol. Two varieties of lettuce were examined, Ljubljanska ledenka and Nansen. Plants were grown in water with phenol concentration of 200 mgL−1 during 10 days. We monitored activity of peroxidases, catalases, polyphenol oxidase and superoxide dismutase, as well as proline and chlorophyll content. We observed a decrease in peroxidases, and increase in activity of catalase, polyphenol oxidase and superoxide dismutase compared to control plants. The concentration of proline was constantly increasing in both lettuce varieties over the course of the experiment. We detected an increase in activity of all monitored enzymes, except polyphenol oxidases, in hairy roots. The hydroponic system provides a useful framework for studying the effect of different harmful substances and its elimination. In such a system, as used in this work for the study of physiological processes in antioxidant protection activated when plant was exposed to phenol, lettuce and its hairy roots can be viewed as tools for water remediation.
Vojin Tadic; Jovan Tadić; Snežana Milošević; Aleksandar Cingel; Olivera Prodanovic; Tatjana Ćosić; Zoran Vujcic. Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)— Part 2. Scientia Horticulturae 2018, 232, 71 -83.
AMA StyleVojin Tadic, Jovan Tadić, Snežana Milošević, Aleksandar Cingel, Olivera Prodanovic, Tatjana Ćosić, Zoran Vujcic. Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)— Part 2. Scientia Horticulturae. 2018; 232 ():71-83.
Chicago/Turabian StyleVojin Tadic; Jovan Tadić; Snežana Milošević; Aleksandar Cingel; Olivera Prodanovic; Tatjana Ćosić; Zoran Vujcic. 2018. "Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)— Part 2." Scientia Horticulturae 232, no. : 71-83.
Expanding from remote areas of Mexico to a worldwide scale, the ten-striped insect, the Colorado potato beetle (CPB, Leptinotarsa decemlineata Say), has risen from being an innocuous beetle to a prominent global pest. A diverse life cycle, phenotypic plasticity, adaptation to adverse conditions, and capability to detoxify or tolerate toxins make this insect appear to be virtually “indestructible”. With increasing advances in molecular biology, tools of biotechnological warfare were deployed to combat CPB. In the last three decades, genetically modified potato has created a new challenge for the beetle. After reviewing hundreds of scientific papers dealing with CPB control, it became clear that even biotechnological means of control, if used alone, would not defeat the Colorado potato beetle. This control measure once again appears to be provoking the potato beetle to exhibit its remarkable adaptability. Nonetheless, the potential for adaptation to these techniques has increased our knowledge of this pest and thus opened possibilities for devising more sustainable CPB management programs.
Aleksandar Cingel; Jelena Savić; Jelica Lazarević; Tatjana Ćosić; Martin Raspor; Ann Smigocki; Slavica Ninković. Extraordinary Adaptive Plasticity of Colorado Potato Beetle: “Ten-Striped Spearman” in the Era of Biotechnological Warfare. International Journal of Molecular Sciences 2016, 17, 1538 .
AMA StyleAleksandar Cingel, Jelena Savić, Jelica Lazarević, Tatjana Ćosić, Martin Raspor, Ann Smigocki, Slavica Ninković. Extraordinary Adaptive Plasticity of Colorado Potato Beetle: “Ten-Striped Spearman” in the Era of Biotechnological Warfare. International Journal of Molecular Sciences. 2016; 17 (9):1538.
Chicago/Turabian StyleAleksandar Cingel; Jelena Savić; Jelica Lazarević; Tatjana Ćosić; Martin Raspor; Ann Smigocki; Slavica Ninković. 2016. "Extraordinary Adaptive Plasticity of Colorado Potato Beetle: “Ten-Striped Spearman” in the Era of Biotechnological Warfare." International Journal of Molecular Sciences 17, no. 9: 1538.
Colorado potato beetle (CPB; Leptinotarsa decemlineata Say, Coleoptera: Chrysomelidae) has shown a remarkable adaptability to a variety of control measures. Although oryzacystatin I and II (OCI and OCII) have potential in controlling pests that use cysteine proteinases for food digestion, expression of a single OC gene in potato exhibited a minimal or no effect on CPB fitness traits. The aim of this study was to examine the effect of coexpressed OCI and OCII in potato (Solanum tuberosum L.) cultivars Desiree, Dragačevka and Jelica on CPB larvae. Growth parameters, consumption rates and food utilization, as well as activity of proteases of CPB larvae were assayed. Second and third instar larvae fed on transformed leaves molted earlier and had higher relative growth and consumption rates than larvae fed on nontransformed leaves, while efficiency of food utilization was unaffected. In contrast, fourth instar maximum weight gain and amount of leaves consumed were about 20% lower for the larvae fed on transgenic potato. Analysis of total protease activity of third instar larvae revealed reduction in overall proteolytic activity measured by azocasein hydrolysis, accompanied with inhibition of cysteine proteinase activity 24 h after ingestion of potato leaves expressing OCI and OCII. However, after long-term feeding on transformed leaves proteolytic activities of larvae became similar to the controls. Although feeding on OCI/OCII leaves did not affect larval survival, coexpression of OC genes reduced the development time and thus significantly decreased plant damage caused by CPB larvae.
Aleksandar Cingel; Jelena Savić; Jelica Lazarević; Tatjana Ćosić; Martin Raspor; Ann Smigocki; Slavica Ninkovic. Co-expression of the proteinase inhibitors oryzacystatin I and oryzacystatin II in transgenic potato alters Colorado potato beetle larval development. Insect Science 2016, 24, 768 -780.
AMA StyleAleksandar Cingel, Jelena Savić, Jelica Lazarević, Tatjana Ćosić, Martin Raspor, Ann Smigocki, Slavica Ninkovic. Co-expression of the proteinase inhibitors oryzacystatin I and oryzacystatin II in transgenic potato alters Colorado potato beetle larval development. Insect Science. 2016; 24 (5):768-780.
Chicago/Turabian StyleAleksandar Cingel; Jelena Savić; Jelica Lazarević; Tatjana Ćosić; Martin Raspor; Ann Smigocki; Slavica Ninkovic. 2016. "Co-expression of the proteinase inhibitors oryzacystatin I and oryzacystatin II in transgenic potato alters Colorado potato beetle larval development." Insect Science 24, no. 5: 768-780.
Dragana Antonic; Snežana Milošević; Aleksandar Cingel; M. Lojić; M. Trifunović-Momčilov; M. Petric; Angelina Subotić; Ana Simonović. Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. South African Journal of Botany 2016, 105, 226 -233.
AMA StyleDragana Antonic, Snežana Milošević, Aleksandar Cingel, M. Lojić, M. Trifunović-Momčilov, M. Petric, Angelina Subotić, Ana Simonović. Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. South African Journal of Botany. 2016; 105 ():226-233.
Chicago/Turabian StyleDragana Antonic; Snežana Milošević; Aleksandar Cingel; M. Lojić; M. Trifunović-Momčilov; M. Petric; Angelina Subotić; Ana Simonović. 2016. "Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought." South African Journal of Botany 105, no. : 226-233.
Plant proteinase inhibitors (PIs) are attractive tools for crop improvement and their heterologous expression can enhance insect resistance in transgenic plants. PI oryzacystatin II (OCII), isolated from rice, showed potential in controlling pests that utilize cysteine proteinases for protein digestion. To evaluate the applicability of the OCII gene in enhancing plant defence, OCII-transformed potatoes were bioassayed for resistance to Colorado potato beetle (Leptinotarsa decemlineata Say). Feeding on transformed leaves of potato cultivars Desiree and Jelica significantly affected larval growth and development, but did not change mortality rates. During the L2 and L3 developmental stages larvae consumed the OCII-transformed foliage faster as compared to the nontransformed control. Also these larvae reached the prepupal stage (end of L4 stage) 2 days earlier than those fed on control leaves. However, the total amounts of consumed OCII-transformed leaves were up to 23 % lower than of control, and the maximal weights of prepupal larvae were reduced by up to 18 % as compared to larvae fed on nontransformed leaves. The reduction in insect fitness reported in this study in combination with other control measures, could lead to improved CPB resistance management in potato.
Aleksandar Cingel; Jelena Savić; Branka Vinterhalter; Dragan Vinterhalter; Miroslav Kostic; Darka Šešlija Jovanović; Ann Smigocki; Slavica Ninkovic. Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor. Transgenic Research 2015, 24, 729 -740.
AMA StyleAleksandar Cingel, Jelena Savić, Branka Vinterhalter, Dragan Vinterhalter, Miroslav Kostic, Darka Šešlija Jovanović, Ann Smigocki, Slavica Ninkovic. Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor. Transgenic Research. 2015; 24 (4):729-740.
Chicago/Turabian StyleAleksandar Cingel; Jelena Savić; Branka Vinterhalter; Dragan Vinterhalter; Miroslav Kostic; Darka Šešlija Jovanović; Ann Smigocki; Slavica Ninkovic. 2015. "Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor." Transgenic Research 24, no. 4: 729-740.
Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple (VP) and Vienna White (VW) were tested for their ability of de novo organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact seedlings were cultivated on Murashige and Skoog (MS) media supplemented with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans- or cis-zeatin. All tested cytokinins, including cis-zeatin, induced shoot regeneration from hypocotyl explants and intact seedlings, with seedlings being most successful for regeneration efficiency and viability of regenerated shoots in both cultivars. The highest frequency of shoot regeneration was achieved on MS with BA (60 %) or TDZ (50 %) for VP; and with BA (50 %), TDZ (47.5 %) or transZ (37.5 %) for VW. Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA) contents in both hypocotyl explants and seedlings with regenerated shoots (HRSs and SRSs) suggested that the observed differences in organogenic response between these two types of explants were related to their cytokinin and IAA contents. HRSs generally exhibited elevated amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive cytokinins ratio. Shoots regenerated from seedlings were further successfully multiplicated on a medium supplemented with BA (0.5 mg L−1). The rooting potential of multiplicated shoots was tested on media supplemented with 2 or 4 mg L−1 indole-3-butyric acid (IBA), with the higher concentration of IBA leading to more efficient rooting. Rooted plantlets were successfully planted into soil and flow cytometric analysis did not reveal ploidy variations, indicating that the described protocol is fast and efficient for kohlrabi regeneration.
Tatjana Ćosić; Václav Motyka; Martin Raspor; Jelena Savić; Aleksandar Cingel; Branka Vinterhalter; Dragan Vinterhalter; Alena Trávníčková; Petre I. Dobrev; Borut Bohanec; Slavica Ninkovic. In vitro shoot organogenesis and comparative analysis of endogenous phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects of genotype, explant type and applied cytokinins. Plant Cell, Tissue and Organ Culture 2015, 121, 741 -760.
AMA StyleTatjana Ćosić, Václav Motyka, Martin Raspor, Jelena Savić, Aleksandar Cingel, Branka Vinterhalter, Dragan Vinterhalter, Alena Trávníčková, Petre I. Dobrev, Borut Bohanec, Slavica Ninkovic. In vitro shoot organogenesis and comparative analysis of endogenous phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects of genotype, explant type and applied cytokinins. Plant Cell, Tissue and Organ Culture. 2015; 121 (3):741-760.
Chicago/Turabian StyleTatjana Ćosić; Václav Motyka; Martin Raspor; Jelena Savić; Aleksandar Cingel; Branka Vinterhalter; Dragan Vinterhalter; Alena Trávníčková; Petre I. Dobrev; Borut Bohanec; Slavica Ninkovic. 2015. "In vitro shoot organogenesis and comparative analysis of endogenous phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects of genotype, explant type and applied cytokinins." Plant Cell, Tissue and Organ Culture 121, no. 3: 741-760.
The evaluation of transgenic plants which is usually carried out under controlled conditions in culture rooms and greenhouses can yield valuable information about the influence of introduced genes on a transgenic plant phenotype. However, an overall assessment of plant performance can only be made by testing transgenic plants in the field environment. Thus, the effects of pyramided rice cystatin genes OCI and OCII on morphological parameters of transgenic potato cv. Desiree, Dragacevka and Jelica lines were compared under in vitro, greenhouse, and field conditions. All analyzed OC co-expressing transgenic lines exhibited normal phenotype, both in vitro and in greenhouse conditions. In the field environment, eight of nine OCI/OCII lines were similar to the wild-type control plants in their general phenotypic appearance. Yield parameters, such as tuber number and tuber weight for these phenotypically normal OCI/OCII lines, were also comparable to the controls. Only transgenic cv. Jelica line 4 plants exhibited slightly reduced growth, atypical leaf morphology and, contrary to the plants of other transgenic lines and untransformed controls, failed to flower. However, despite the phenotypic and developmental changes under field conditions, the OCI/OCII Jelica line 4 did not exhibit a significant decrease in tuber yield. Stacking of OCI and OCII genes preserves important attributes of the parental lines, confirming that this approach could be suitable for improving agronomical traits in potato.
Aleksandar Cingel; Jelena Savic; Tatjana Cosic; Martin Raspor; Nabil Ghalawenji; Ann Smigocki; Slavica Ninkovic. Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII). Archives of Biological Sciences 2015, 67, 957 -964.
AMA StyleAleksandar Cingel, Jelena Savic, Tatjana Cosic, Martin Raspor, Nabil Ghalawenji, Ann Smigocki, Slavica Ninkovic. Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII). Archives of Biological Sciences. 2015; 67 (3):957-964.
Chicago/Turabian StyleAleksandar Cingel; Jelena Savic; Tatjana Cosic; Martin Raspor; Nabil Ghalawenji; Ann Smigocki; Slavica Ninkovic. 2015. "Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)." Archives of Biological Sciences 67, no. 3: 957-964.
Integration of desirable traits into commercial ornamentals using genetic engineering techniques is a powerful tool in contemporary biotechnology. However, these techniques have had a limited impact in the domain of ornamental horticulture, particularly floriculture. Modifications of the color, architecture or fragrance of the flowers as well as an improvement of the plant tolerance/resistance against abiotic and biotic stresses using plant transformation techniques, is still in its infancy. This review focuses on the application of Agrobacterium-mediated transformation, a major plant genetic engineering approach to ornamental plant breeding and the impact it has had to date.
Snezana Milosevic; Aleksandar Cingel; Angelina Subotic. Agrobacterium-mediated transformation of ornamental species: A review. Genetika 2015, 47, 1149 -1164.
AMA StyleSnezana Milosevic, Aleksandar Cingel, Angelina Subotic. Agrobacterium-mediated transformation of ornamental species: A review. Genetika. 2015; 47 (3):1149-1164.
Chicago/Turabian StyleSnezana Milosevic; Aleksandar Cingel; Angelina Subotic. 2015. "Agrobacterium-mediated transformation of ornamental species: A review." Genetika 47, no. 3: 1149-1164.
Impatiens walleriana L. shoots were inoculated with Agrobacterium rhizogenes A4M70GUS and the effects of genetic transformation on the catalase (CAT), superoxide dismutase (SOD) and peroxidase (POX) activities in wounded region of stems and unwounded leaves were evaluated 10, 24, 240 and 720 hours after inoculation. Following Agrobacterum infection activities of plant antioxidative enzymes changed in a time-dependent manner indicating that dynamic processes occurred during plant-Agrobacterium interaction, plant cell transformation and formation of hairy roots. Appearance of hairy roots on wound sites of shoots was observed ten days after inoculation with A. rhizogenes and the root induction frequency was 100%. Among selected hairy root lines significant differences in growth rate and biomass production were observed and an average 3-fold increase in biomass production was observed for the best growing hairy root line compared with the untransformed roots. PCR analysis showed presence of uidA, rolB, rolC and rolD genes in all analyzed I. walleriana L. hairy root lines, while amplification fragment of rolA gene was detected in 83.3% transformed lines. Efficient transformation protocol for I. walleriana L described in this work offer possibilities to generate hairy root cultures for in vitro propagation of plant viruses.
Snezana Milosevic; Milena Lojic; Dragna Antonić; Aleksandar Cingel; Angelina Subotic. Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation. Genetika 2015, 47, 71 -84.
AMA StyleSnezana Milosevic, Milena Lojic, Dragna Antonić, Aleksandar Cingel, Angelina Subotic. Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation. Genetika. 2015; 47 (1):71-84.
Chicago/Turabian StyleSnezana Milosevic; Milena Lojic; Dragna Antonić; Aleksandar Cingel; Angelina Subotic. 2015. "Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation." Genetika 47, no. 1: 71-84.
The plant hormones cytokinins (CKs) regulate a number of physiological processes. Their homeostasis is controlled by the rate of de novo synthesis and the rate of catabolism. The aim of this work was to analyze the content of total as well as individual groups of endogenous CKs in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro. Transgenic CKX plants represent a suitable model system for studying physiological and morphological processes controlled by CKs. In this work we clearly demonstrate a significant effect of AtCKX transgenes on CK metabolism in transgenic centaury plants. However, shoots and roots of only one AtCKX1 line and three AtCKX2 lines with a significant reduction of bioactive CKs were obtained. We also show that changes in the CKs metabolism considerably affected endogenous indole-3-acetic acid (IAA) levels in plant tissues. All analyzed transgenic AtCKX centaury lines exhibited decreased amount of endogenous IAA in shoots as well as in roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation in the shoots and roots of all analyzed AtCKX centaury transformants.
Milana Trifunović; Václav Motyka; Aleksandar Cingel; Angelina Subotic; Slađana Jevremović; Marija Petric; Josef Holík; Jiří Malbeck; Petre I. Dobrev; Ivana Č. Dragićević; Sladjana Jevremović. Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro. Plant Cell, Tissue and Organ Culture (PCTOC) 2014, 120, 767 -777.
AMA StyleMilana Trifunović, Václav Motyka, Aleksandar Cingel, Angelina Subotic, Slađana Jevremović, Marija Petric, Josef Holík, Jiří Malbeck, Petre I. Dobrev, Ivana Č. Dragićević, Sladjana Jevremović. Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro. Plant Cell, Tissue and Organ Culture (PCTOC). 2014; 120 (2):767-777.
Chicago/Turabian StyleMilana Trifunović; Václav Motyka; Aleksandar Cingel; Angelina Subotic; Slađana Jevremović; Marija Petric; Josef Holík; Jiří Malbeck; Petre I. Dobrev; Ivana Č. Dragićević; Sladjana Jevremović. 2014. "Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro." Plant Cell, Tissue and Organ Culture (PCTOC) 120, no. 2: 767-777.
The effects of Chenopodium murale root exudates, applied as phytotoxic medias (PMs), were tested on Arabidopsis thaliana and Triticum aestivum. The effects of PMs, where wild-type roots (K), hairy roots derived from roots (R clones) or from cotyledons (C clones) were cultured, were different. K medium suppressed Arabidopsis germination, while other PMs reduced root and leaf elongation and the number of rosette leaves. R media were more phytotoxic than C media. Treatment of Arabidopsis with R8 down-regulated expression of core cell cycle genes: cyclin-dependent kinase (CDK) A1;1, four B-class CDKs, and cyclins CYCA3;1, CYCB2;4, CYCD4;2 and CYCH1 in root and shoot tips. Only CYCD2;1 transcript was elevated in treated shoots, but down-regulated in roots. Wheat Ta-CDC2 and Ta-CYCD2 genes showed the same expression profiles as their Arabidopsis counterparts, CDKA1;1 and CYCD2;1. PMs also caused increase of antioxidative enzyme activities in both plants. Exposure of Arabidopsis to PMs induced one catalase isoform, but repressed another, resulting in no net change of catalase activity. Wheat seedlings treated with PMs had catalase activity significantly elevated in all treatments, particularly in shoots. In both plants, PMs induced the activity of different peroxidase isozymes and total peroxidase activity. Both plants responded to phytotoxic treatments by induction of CuZn-superoxide dismutase. Thus, the phytotoxicity of C. murale root exudates is, at least partially, based on down-regulation of the cell cycle regulators and on generation of oxidative stress in the affected plants. We propose that C. murale root exudates should be considered as means of biological weed control.
Slavica Dmitrović; Ana Simonovic; Nevena Mitić; Jelena Savić; Aleksandar Cingel; Biljana Filipovic; Slavica Ninkovic. Hairy root exudates of allelopathic weed Chenopodium murale L. induce oxidative stress and down-regulate core cell cycle genes in Arabidopsis and wheat seedlings. Plant Growth Regulation 2014, 75, 365 -382.
AMA StyleSlavica Dmitrović, Ana Simonovic, Nevena Mitić, Jelena Savić, Aleksandar Cingel, Biljana Filipovic, Slavica Ninkovic. Hairy root exudates of allelopathic weed Chenopodium murale L. induce oxidative stress and down-regulate core cell cycle genes in Arabidopsis and wheat seedlings. Plant Growth Regulation. 2014; 75 (1):365-382.
Chicago/Turabian StyleSlavica Dmitrović; Ana Simonovic; Nevena Mitić; Jelena Savić; Aleksandar Cingel; Biljana Filipovic; Slavica Ninkovic. 2014. "Hairy root exudates of allelopathic weed Chenopodium murale L. induce oxidative stress and down-regulate core cell cycle genes in Arabidopsis and wheat seedlings." Plant Growth Regulation 75, no. 1: 365-382.