Background: Colorectal cancer (CRC) has high incidence and mortality rates, with severe prognoses during invasion and metastasis stages. Despite advancements in diagnostic and therapeutic technologies, the impact of the tumour microenvironment, particularly extracellular matrix (ECM) stiffness, on CRC progression and metastasis is not fully understood. Methods: This study included 107 CRC patients. Tumour stiffness was assessed using magnetic resonance elastography (MRE), and collagen ratio was analysed with Masson staining. CRC cell lines were cultured on matrices of varying stiffness, followed by transcriptome sequencing to identify stiffness-related genes. An HSF4 knockout CRC cell model was cultured in different ECM stiffness to evaluate the effects of HSF4 on cell proliferation, migration, and invasion in vitro and in vivo. Results: CRC tumour stiffness was significantly higher than normal tissue and positively correlated with collagen content and TNM staging. High-stiffness matrices significantly regulated cell functions and signalling pathways. High HSF4 (heat shock transcriptional factor 4) expression was strongly associated with tumour stiffness and poor prognosis. HSF4 expression increased with higher TNM stages, and its knockout significantly inhibited cell proliferation, migration, and invasion, especially on high-stiffness matrices. In vivo experiments confirmed that HSF4 promoted tumour growth and metastasis, independent of collagen protein increase. Conclusions: This study reveals that tumour stiffness promotes the proliferation and metastasis of CRC by regulating EMT-related signalling pathways through HSF4. Tumour stiffness and HSF4 could be valuable targets for prognostic assessment and therapeutic intervention in CRC.

To adjust the thermal safety of hydrophobic silica aerogel, layered double hydroxide (LDH)/silica aerogel (SA) composites were prepared by an in-situ sol-gel process at ambient pressure. This study found the physical combination of SA and MgAl-LDH based on the FTIR spectra and phase composition of LDH/SA. The N₂ sorption analysis confirms that the introduction of MgAl-LDH does not change the mesoporous attribution of LDH/SA significantly. With the increase in MgAl-LDH addictive content, the low density (0.12–0.13 g/cm³), low thermal conductivity (24.28–26.38 mW/m/K), and large specific surface area (730.7–903.7 m²g) of LDH/SA are still maintained, which can satisfy the requirements of thermal insulation. The TG-DSC analysis demonstrates that the endothermic effects and metal oxides formed during the MgAl-LDH decomposition are beneficial to the improvement of the thermal stability of LDH/SA composites. In addition, it was found that the gross calorific values of LDH/SA composites decrease with an increase in MgAl-LDH addictive content, all of which are lower than that of the pure SA. The research outcomes indicate that the thermal safety of LDH/SA composites is enhanced significantly by doping MgAl-LDH without impairing too many of the excellent properties, which benefits their expansion in the thermal insulation field.

Toxoplasma gondii infection is a severe health threat that endangers billions of people worldwide. T. gondii utilizes the host cell membrane to form a parasitophorous vacuole (PV), thereby fully isolating itself from the host cell cytoplasm and making intracellular clearance difficult. PV can be targeted and destroyed by autophagy. Autophagic targeting results in T. gondii killing via the fusion of autophagosomes and lysosomes. However, T. gondii has developed many strategies to suppress autophagic targeting. Accordingly, the interplay between host cell autophagy and T. gondii is an emerging area with important practical implications. By promoting the canonical autophagy pathway or attenuating the suppression of autophagic targeting, autophagy can be effectively utilized in the development of novel therapeutic strategies against T gondii. Here, we have illustrated the complex interplay between host cell mediated autophagy and T. gondii. Different strategies to promote autophagy in order to target the parasite have been elucidated. Besides, we have analyzed some potential new drug molecules from the DrugBank database using bioinformatics tools, which can modulate autophagy. Various challenges and opportunities focusing autophagy mediated T. gondii clearance have been discussed, which will provide new insights for the development of novel drugs against the parasite.

Chemical dust suppression is an effective dust control technology. A dust suppressant component evaluation method that facilitates a complete selection of safe, efficient, and economical chemical materials has not been explored. Considering dust suppression performance, environmental safety, and cost-effectiveness of chemical dust suppressant technology, this study constructs a comprehensive evaluation index system of chemical dust suppressant performance, including the wetting performance, hygroscopic performance, bonding performance, annual cost per unit area, pH value of dust suppression solution, chemical toxicity, and chemical corrosion. Among them, the index characterizing the wetting performance of the solution is the sedimentation wetting time, which is determined by the dust sedimentation experiment; the index characterizing the hygroscopic performance of the solution is the evaporation stability time, which is determined by the evaporation experiment of the solution on the dust surface; the index to characterize the bonding performance of the solution is the surface wind erosion rate, which is determined by the wind erosion experiment of the solution on the dust surface; the toxicity of the solution is evaluated by the LD50 of the solution; the index to characterize the corrosion performance of the solution is the Q235 monthly steel corrosion rate, which is determined by the Q235 steel corrosion test. Corresponding evaluation parameters are determined including sedimentation wetting time, evaporation stabilization time, surface wind erosion rate; annual average use cost per unit area; solution pH value, chemical acute toxicity classification, monthly corrosion rate of Q235 steel, and corresponding standard test methods are also provided. In order to evaluate the comparability of the results, according to the specific requirements of the evaluation index system and the distribution characteristics of the measurement data, the data of each evaluation and detection index are standardized by linear transformation, range transformation and other methods, so that the obtained results are comparable. Considering the differences in the actual performance requirements of dust suppressants in different usage scenarios, the weights of evaluation indicators at all levels can be set independently and flexible. The experimental test data obtained through the example shows that: among the four chemicals selected to participate in the experiment, the comprehensive dust suppression performance score of Triton X-100 solution is in the poor-grade category. The comprehensive dust suppression performances of calcium chloride solution, water, and polyacrylamide solution scored high in the average-grade category. The comprehensive evaluation process is logically correct, and the results are consistent with the phenomena observed in the experiment, consistent with conventional understanding, and have strong credibility. This method can provide a standardized evaluation technique and test process for the comprehensive performance evaluation and comparison of chemical materials and dust suppressants.

As a strategic emerging mineral resource, lithium is widely used in new energy, new materials and other emerging industries. There exists a changing trend of the material flow, consumption and evolution of lithium resources in the market. Thus, this research constructed a material flow analysis system for lithium resources based on the trade correlation of the whole life cycle. The study used the material flow analysis method to analyze the supply, flow and stock of lithium resources in China from 2007 to 2020. The research shows that during that timeframe, China’s cumulative consumption of lithium resources equivalent to lithium carbonate reached 309.9348 kiloton (kt). The consumption of lithium in traditional and lithium electric fields increased from 14.3653 kt and 8.08228 kt in 2007 to 49.53125 kt and 90.75866 kt in 2020, respectively. From 2007 to 2011, the consumption of lithium in the traditional sector was greater than that in the lithium electric sector. From 2012 to 2020, with the innovation of network technology and the boom in the New energy vehicles (NEV) market, the market for consumer lithium and power lithium grew rapidly, and the field of lithium with power batteries as the main driving force gradually became the major contributor to the consumption of lithium resources. With the rapid increase in the consumption demand for lithium resources, the supply structure of lithium resources in China has changed from domestic supply to international import. The external dependence of lithium resources has increased from 29.74% in 2007 to 70.75% in 2020. With increasing lithium consumption, the storage of lithium batteries increased from 20.69721 kt in 2007 to 341.6322 kt in 2020. At the same time, the scrap volume of lithium batteries increased rapidly, but the recycling volume of lithium resources was far lower than the scrap volume. The resource recycling potential is huge, and there is still a lot of room for the development of the sequential utilization of waste lithium-ion batteries.