Haematococcus pluvialis is the richest biological source of astaxanthin under unfavorable growing conditions. Many reports have discussed the optimal astaxanthin extraction methods. Free-astaxanthin could be still hindered by microalgae extracts composition or by prolonged extraction times. In this study we evaluated the effect of enzymolysis and saponification deesterification processes of astaxanthin and its carotenoid precursors under high irradiance and nitrogen deprivation stress time conditions. Results showed that cholesterol esterase facilitated astaxanthin deesterification (975.65 μg mg-1 DW) while saponification positively affected zeaxanthin (1038.68 μg mg-1 DW).

The protein and polyunsaturated fatty acid (PUFA) enrichment of microalgae can improve their nutritional value for larvae of various reared organisms. Protein enrichment can be achieved by increasing nitrogen concentration and selecting nitrogen sources that are easy to assimilate during microalga culture. Nitrogen-rich cultures can increase organism growth, biomass, and protein content, but their lipid content tends to stall. Since the diatom Thalassiosira weissflogii is usually provided to feed shrimp larvae, this study evaluated its digestibility and biochemical composition, culturing it with two nitrogen sources (NaNO₃ and NH₄Cl) at different concentrations (111.25, 222.50, 445 and 890 µM). The cell abundance, dry-weight biomass, Chl a, proteins, carbohydrates, total lipids and essential fatty acids were determined. The cell density and biomass peaked faster (day 12) with treatment < 890 µM than with 890 µM (day 15) in both nitrogen sources. However, the highest cell density, biomass and peak protein yield were not significantly different among treatments, suggesting the need to compare maintenance costs for a given production. After nine days of culture, concentrations ≤ 222.5 µM increased lipid content irrespective of the nitrogen source and decreased by 10–20% afterwards. With higher lipid production, the dominant PUFA were eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). One gram of NH₄Cl provides ~60% more nitrogen than 1 g of NaNO₃. In conclusion, based on time and growth rate, T. weissflogii cultivated with NH₄Cl at 222.50 µM produced EPA and DHA at a better yield–cost ratio for biomass and lipid production. Furthermore, its nutritional value as enriched live-food for the reared larvae of marine organisms suggests potential biotechnological applications for aquaculture.

Eutrophication-resistant phytoplankton communities in freshwater ecosystems have a novel lipid potential to contribute to the development of tropical regions. The question that arises due to the unsustainability of their eutrophicated waters is how the recognition of the lipids of the resident phytoplankton progresses. Our aim was to provide an overview of the pico-, nano- and micro-cellular lipids of phytoplankton with a focus on eutrophic tropical freshwater ecosystems. Using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) framework, global and Latin American publications were retrieved based on search equations and specific questions. In total, 490 studies were reviewed. The inclusion criteria, in order, were (1) peer-reviewed articles, (2) articles investigating phytoplankton strains or communities from any aquatic environment, (3) articles on freshwater ecosystems, and (4) research in tropical climates. The contribution of freshwater phytoplankton was high and discontinuous, with a representation of 63% in the 21st century. Freshwater themes were resolved in the ecological context with phytoplankton or algae keywords, while microalgae were targeted using resource use keywords. On the tropical scale, technological themes on lipid microalgae were related to fatty acids, biofuels, biodiesel, antioxidants, and recombinant DNA. It is concluded that studies of the lipid composition of phytoplankton communities are delayed in the case of eutrophic tropical freshwater ecosystems.