Nitrogen fertilization and planting density are two key factors that can interactively affect the grain yield of rice. Three different types of rice cultivars—inbred Shendao 47, inbred Shendao 505, and hybrid Jingyou 586—were applied to investigate the effects of the nitrogen (N) rate and planting density (D) on the aboveground biomass, harvest index, leaf photosynthetic features, grain yield, and yield components using a split-split-plot design at two sites over two continuous years. The main plots were assigned to four nitrogen fertilizer rates: 0 (N0), 140 (N1), 180 (N2), and 220 (N3) kg ha⁻¹ N; the subplots were assigned to three planting densities: 25 × 10⁴ (D1), 16.7 × 10⁴ (D2), and 12.5 × 10⁴ (D3) hills ha^-1, and the sub-subplots were assigned to three rice cultivars. The results showed that the grain yield had a significantly positive correlation with the stomatal conductance (Gs), net photosynthesis rate (Pn), transpiration rate (Tr), chlorophyll content (SPAD value), leaf area index (LAI), panicles per unit area, and spikelets per panicle. The N rate and planting density had significant interaction effects on grain yield, and the maximum values of Shendao 47, Shendao 505, and Jingyou 586 appeared in N3D2, N2D1, and N3D3, respectively. The higher grain yield of midsized panicle Shendao 47 was mostly ascribed to both panicles per unit area and spikelets per panicle. More panicles per unit area and spikelets per panicle primarily contributed to a larger sink capacity of small-sized panicle rice Shendao 505 and large-sized panicle rice Jingyou 586. We found that the treatments N3D2, N2D1, and N3D3 could optimize the contradiction between yield formation factors for Shendao 47, Shendao 505, and Jingyou 586, respectively. Across years and sites, the regression analysis indicated that the combinations of nitrogen fertilization of 195.6 kg ha⁻¹ with a planting density of 22 × 10⁴ hills ha⁻¹, 182.5 kg ha⁻¹ with 25 × 10⁴ hills ha⁻¹, and 220 kg ha⁻¹ with 13.1 × 10⁴ hills ha⁻¹ are recommended for medium-, small-, and large-sized panicle rice cultivars, respectively.

In order to investigate the feasibility of using rice leaf critical nitrogen concentration as a nitrogen nutrition diagnosis index, a three-year positioning experiment with large-spike rice cultivar (Wuyoudao4) and multiple-spike rice cultivar (Songjing9) under five nitrogen levels (0, 60, 120, 180, and 240 kg·ha⁻¹) was conducted. A critical nitrogen dilution curve and a nitrogen nutrition index (NNI) of rice leaf dry matter were constructed for Wuyoudao4 (N_c = 1.96L_DM^−0.56, R² = 0.87, NNI was between 0.6–1.26, and Normalized Root Mean Square Error (n-RMSE) = 13.07%) and Songjing9 (N_c = 1.99L_DM^−0.44, R² = 0.94, NNI was between 0.64–1.29, and n-RMSE = 15.89%). The relationship between dry matter and nitrogen concentration of rice leaves was a negative power function, and the model had good stability over the three years. The developed critical nitrogen concentration dilution curve, based on leaf dry matter, was able to diagnose nitrogen nutrition in rice efficiently. The model established in this study could be used to directly regulate and control the nitrogen nutrition of rice leaves.

Dry direct seeding rice (DSR) is an emerging production system because of increasing labor and water scarcity in rice cultivation. The limited availability of rice cultivars suitable for dry direct seeding hampers the widespread adoption of this cultivation method in Northeast China. This study aimed to investigate grain production and plant characteristics associated with dry direct seeding rice. We conducted a field experiment on 79 japonica rice cultivars in Shenyang City, Liaoning Province, Northeast China, in 2020 and 2021. This study found that the grain yield of the tested rice cultivars ranged from 5.75–11.00 t ha⁻¹, with a growth duration lasting between 144–161 days across the cultivars. These cultivars were then categorized into high yielding (HY), medium yielding (MY), and low yielding (LY) based on daily yield by using Ward’s hierarchical clustering method. The higher grain yield for HY compared to MY and LY was attributed to more spikelets per unit area. The HY alleviated the conflict between higher panicle density and larger panicle size by improving the seedling emergence rate and productive stem rate. It also significantly increased shoot biomass at maturity. The HY reduced the period between seeding and beginning of heading (BBCH 51) and the proportion of dry matter partitioned to the leaf at the heading stage. However, it also increased the accumulation of dry matter in the grain and the proportion of dry matter partitioned to the grain at maturity. Furthermore, the HY markedly increased the harvest index and grain-leaf ratio, which are beneficial to coordinate the source–sink relationship. A quadratic function predicted that 98 days is the optimum growth duration before heading (BBCH 51) for achieving maximum yield. In conclusion, for dry direct seeding rice, it is appropriate to select high-yielding japonica inbred rice cultivars with shorter growth duration before heading (about 93–102 day), higher panicle number (about 450–500 × 10⁴ ha^–1), more spikelet number per panicle (about 110–130), higher seedling emergence rate (about 70–75%), higher productive stem rate (about 60–70%), and greater harvest index (about 50–55%).

Grain size and flag leaf angle are two important traits that determining grain yield in rice. However, the mechanisms regulating these two traits remain largely unknown. In this study, a rice long grain 5 (lg5) mutant with a large flag leaf angle was identified, and map-based cloning revealed that a single base substitution followed by a 2 bp insertion in the LOC_Os05g40384 gene resulted in larger grains, a larger flag leaf angle, and higher plant height than the wild type. Sequence analysis revealed that lg5 is a novel allele of elongated uppermost internode-1 (EUI1), which encodes a cytochrome P450 protein. Functional complementation and overexpression tests showed that LG5 can rescue the bigger grain size and larger flag leaf angle in the Xiushui11 (XS) background. Knockdown of the LG5 transcription level by RNA interference resulted in elevated grain size and flag leaf angle in the Nipponbare (NIP) background. Morphological and cellular analyses suggested that LG5 regulated grain size and flag leaf angle by promoting cell expansion and cell proliferation. Our results provided new insight into the functions of EUI1 in rice, especially in regulating grain size and flag leaf angle, indicating a potential target for the improvement of rice breeding.

Grain size, grain number per panicle, and grain weight are key agronomic traits that determine grain yield in rice. However, the molecular mechanisms coordinately controlling these traits remain largely unknown. In this study, we identified a major QTL, SMG3, that is responsible for grain size, grain number per panicle, and grain weight in rice, which encodes a MYB-like protein. The SMG3 allele from M494 causes an increase in the number of grains per panicle but produces smaller grain size and thousand grain weight. The SMG3 is constitutively expressed in various organs in rice, and the SMG3 protein is located in the nucleus. Microscopy analysis shows that SMG3 mainly produces long grains by increasing in both cell length and cell number in the length direction, which thus enhances grain weight by promoting cell expansion and cell proliferation. Overexpression of SMG3 in rice produces a phenotype with more grains but reduces grain length and weight. Our results reveal that SMG3 plays an important role in the coordinated regulation of grain size, grain number per panicle, and grain weight, providing a new insight into synergistical modification on the grain appearance quality, grain number per panicle, and grain weight in rice.