The spread of invasive pests is accelerated by globalization and changes in climate conditions, posing a significant threat to agricultural and forest ecosystems. Advances in electronic nose sensors (e-noses) have opened new avenues for monitoring and detecting plant diseases and pests through the analysis of emitted volatile organic compounds (VOCs). The current work reviews the most recent developments in e-nose sensors and their application in plant disease and pest detection over the past five years. It also explores the challenges associated with VOC detection in agricultural settings where field sampling has a focal role in monitoring and management.

Archeobotanical materials recovered from pottery vessels originating from the underwater archeological site of “Gran Carro”, located in Central Italy on the shore of Bolsena Lake, were analyzed to obtain new insight into the agricultural habits present in this Iron Age settlement. The archeobotanical study of cereal remains was combined with analytical data obtained from an amorphous organic residue using optical microscopy, SEM-EDS, ATR/FT-IR and Py-GC/MS. The cereal remains of emmer wheat (Triticum dicoccum), barley (Hordeum vulgare), broomcorn millet (Panicum miliaceum), and foxtail millet (Setaria italica) were identified as the preferred crops used for food and/or fodder at the site. The presence of charred millets, which have been directly dated by AMS, confirms consumption at the site and adds to the little-known background of millet use in central Italy. The find of millets in a perilacustrine pile-dwelling during a period when the water level of the Bolsena Lake was several meters lower than at present, attesting to a general dry period, suggests that the cultivation of millets, complementing more productive crops of wheat and barley, may have been favored by the availability of a large seasonally dry coastal plain, characterized by poor and sandy soils unsuitable for more demanding cereals.

The study of the urban context in the contemporary center of Portugal’s capital city uncovered traces of daily lives that were abruptly interrupted and utterly transformed by the Great Lisbon Earthquake on the morning of 1 November 1755. Charred organic residue was recovered from a cylindrical vessel excavated from the storage area of the town house at the Rossio square. The archaeological sample was studied through a multi-analytical approach based on microstructural, elemental and biomolecular characterization by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR), variable pressure scanning electron microscopy coupled to energy dispersive X-ray spectroscopy (VP-SEM-EDS), and gas chromatography coupled with mass spectrometry (GC-MS). The residue was identified as human faeces collected in the ceramic vessel for disposal, and further analysis provided additional information about diet and the living conditions in the 18th century.

Among the most concerning threats impacting global forest ecosystems is the pinewood nematode (Bursaphelenchus xylophilus (Steiner and Buhrer, 1934) Nickle, 1970), the causal agent of pine wilt disease. In Europe, effective management of this pest requires comprehensive regulatory and monitoring strategies, including the annual collection of thousands of wood samples from symptomatic trees and their surroundings, inspection of wood packaging materials like pallets, and the trapping of the insect vector, Monochamus spp., through national networks. Insects and wood samples are sent to official laboratories, where the latter are sometimes incubated at 25 °C for 15 days, aiming to maximize the probability of the detection of the nematode. This study expected to elucidate the effect of the wood incubation process on the detection of B. xylophilus by analyzing wood samples from pallets and green wood obtained from pine stands, both harbouring nematodes in adult and juvenile stages. Additionally, the investigation sought to assess how the presence of fungi, which serve as a food source for the nematodes, enables B. xylophilus to persist in treated pallet wood that is colonized by these fungi. The results indicated that the incubation period is unnecessary for detecting B. xylophilus in pallets, except when the wood is heavily colonized by fungi providing suitable nutrition for the nematodes, although such occurrences are expected to be rare. Furthermore, this study found no significant differences in population growth between the two stages of the nematode’s life cycle. This suggests that second-stage juveniles present in wood samples, despite not undergoing sexual differentiation, do not hinder the reproductive capacity of B. xylophilus. The risk of a potential infestation in treated pallet wood is unlikely if the treatment has been performed correctly, and the incubation does not contribute to increasing the probability of detecting the PWN. Conversely, for samples obtained from trees, the incubation period significantly enhances nematode detection.

Pinus brutia Ten. is distributed throughout the Eastern Mediterranean, mainly in Turkey and bordering countries. Pine essential oils (EOs), mainly comprised of terpenoid volatiles, have high commercial value and a wide range of biological applications as repellents, insecticides, antivirals, antimicrobials and antioxidants. The present work reviewed the chemical variability of EOs reported for P. brutia trees and related bioproducts. The major EO components (≥20%) reported were α-pinene, β-pinene and δ-3-carene, which generally comprised 50–90% of the EO composition. δ-3-Carene showed the highest variability, suggesting the occurrence of chemotypes. Assessing the variability of EOs extracted from different tree parts or tree bioproducts can provide useful information for guided P. brutia EO extraction according to its intended purpose.