Leptin is a regulator of body weight and affects nitric oxide (NO) production. This study was designed to determine whether the myocardial NO-cGMP signal transduction system was altered in leptin-deficient obese mice. Contractile function, guanylyl cyclase activity, and cGMP-dependent protein phosphorylation were assessed in ventricular myocytes isolated from genetically obese (B6.V-Lepob) and age-matched lean (C57BL/6J) mice. There were no differences in baseline contraction between the lean and obese groups. After stimulation with the NO donor S-nitroso- N-acetyl-penicillamine (SNAP, 10–6and 10–5M) or a membrane-permeable cGMP analog 8-bromo-cGMP (8-Br-cGMP, 10–6and 10–5M), cell contractility was depressed. However, 8-Br-cGMP had significantly greater effects in obese mice than in lean controls with percent shortening reduced by 47 vs. 39% and maximal rate of shortening decreased by 46 vs. 36%. The negative effects of SNAP were similar between the two groups. Soluble guanylyl cyclase activity was not attenuated. This suggests that the activity of the cGMP-independent NO pathway may be enhanced in obesity. The phosphorylated protein profile of cGMP-dependent protein kinase showed that four proteins were more intensively phosphorylated in obese mice, which suggests an explanation for the enhanced effect of cGMP. These results indicate that the NO-cGMP signaling pathway was significantly altered in ventricular myocytes from the leptin-deficient obese mouse model.

The paper presents research on increasing the efficiency of industrial centrifugal fans, which are the third highest energy-consuming group of devices. This problem is related to the frequent incompatibility of the fan characteristics with the flow conditions of the installation in which they are operated, which lowers efficiency and increases energy consumption. By ensuring high efficiency of these devices, it is possible to significantly reduce the operating costs of such industrial equipment. For this purpose, a unique concept of regulating the flow parameters of centrifugal fans was developed. The scientific basis of this idea is that a relatively small change in the impeller diameter of the fan significantly affects the flow rate and the pressure rise of the fan. Therefore, the idea uses variable length of the impeller blades by dividing them into a fixed and a movable part. It enables adjustment of the length of the blades, which allows extension of the range of effective and efficient operating parameters. On this basis, several technical solutions of the regulation system were developed, which were then tested using simulation and analytical methods. Two material options for the moving part of the blade have also been developed, one made of metallic materials (high-strength steel and aluminum) and the other made of composite materials. The research showed a significant influence of the mass of the movable part of the blade on the load of the control system. Therefore, ultra-light material variants were used for the impeller blades. The flow simulation tests of the new method of adjusting the fan flow parameters confirmed the effectiveness of this solution. As a result, a wider range of highly efficient centrifugal fans equipped with such a control system was obtained.