Jasmonates (JAs) are lipid-derived compounds that function in plants as key signaling compounds during stressful conditions. This study aimed to examine the effects of exogenous fo-liar-JA application (100 μmol L⁻¹) on the morpho-physiological response of two soybean varieties (parachinar-local and swat-84) grown under different NaCl regimes (0, 40, 80, and 120 mM). Results show that exogenous JA application alone and in combination with salt stress altered the growth and metabolism of both soybeans. For instance, they accumulated significant amounts of Na⁺ and Cl^–, while their K⁺, Mg²⁺, Fe²⁺, Mn²⁺, B³⁺, and P³⁺ contents were low. Further, photosynthetic pigments Chl a and Chl b increased at low concentrations of salt and exogenous JA. Car decreased under both salt and exogenous JA as compared with untreated control. In addition, sugar, phenol, and protein content increased under both salt and exogenous JA application. In contrast, the exogenous JA application alleviated the negative impact of salt stress on the growth and metabolism of both soybeans. Further, the high concentrations of soluble protein and phenol in the leaves of both soybeans may contribute to their ability to adapt to salinity. However, molecular studies are necessary to understand the ameliorative role of exogenous JA in the growth and metabolism of salt-treated young seedlings in both soybean varieties.

Background: Nitrogen (N) availability is crucial in regulating plants’ abiotic stress resistance, particularly at the seedling stage. Nevertheless, plant responses to N under salinity conditions may vary depending on the soil’s NH₄⁺to NO₃⁻ratio.Methods: In this study, we investigated the effects of different NH₄⁺:NO₃⁻ratios (100/0, 0/100, 25/75, 50/50, and 75/25) on the growth and physio-biochemical responses of soybean seedlings grown under controlled and saline stress conditions (0-, 50-, and 100-mM L^− 1NaCl and Na₂SO₄, at a 1:1 molar ratio).Results: We observed that shoot length, root length, and leaf-stem-root dry weight decreased significantly with increased saline stress levels compared to control. Moreover, there was a significant accumulation of Na⁺, Cl⁻, hydrogen peroxide (H₂O₂), and malondialdehyde (MDA) but impaired ascorbate-glutathione pools (AsA-GSH). They also displayed lower photosynthetic pigments (chlorophyll-a and chlorophyll-b), K⁺ion, K⁺/Na⁺ratio, and weakened O₂^•−-H₂O₂-scavenging enzymes such as superoxide dismutase, catalase, peroxidase, monodehydroascorbate reductase, glutathione reductase under both saline stress levels, while reduced ascorbate peroxidase, and dehydroascorbate reductase under 100-mM stress, demonstrating their sensitivity to a saline environment. Moreover, the concentrations of proline, glycine betaine, total phenolic, flavonoids, and abscisic acid increased under both stresses compared to the control. They also exhibited lower indole acetic acid, gibberellic acid, cytokinins, and zeatine riboside, which may account for their reduced biomass. However, NH₄⁺:NO₃⁻ratios caused a differential response to alleviate saline stress toxicity. Soybean seedlings supplemented with optimal ratios of NH₄⁺:NO₃⁻(T3 = 25:75 and T = 4 50:50) displayed lower Na⁺and Cl⁻and ABA but improved K⁺and K⁺/Na⁺, pigments, growth hormones, and biomass compared to higher NH₄⁺:NO₃⁻ratios. They also exhibited higher O₂^•−-H₂O₂-scavenging enzymes and optimized H₂O₂, MDA, and AsA-GSH pools status in favor of the higher biomass of seedlings.Conclusions: In summary, the NH₄⁺ and NO₃⁻ratios followed the order of 50:50 > 25:75 > 0:100 > 75:25 > 100:0 for regulating the morpho-physio-biochemical responses in seedlings under SS conditions. Accordingly, we suggest that applying optimal ratios of NH₄⁺and NO₃⁻(25/75 and 50:50) can improve the resistance of soybean seedlings grown in saline conditions.

The emergence of chemoresistant cancers and toxicity related to existing chemotherapeutic agents, demand the search for new pharmacophore with enhanced anti-cancer activity and least toxicity. For this purpose, three new sesquiterpenes were isolated from ethyl acetate fraction of the aerial parts of the plant Polygonum barbatum and evaluated for their anti-cancer potential. The structural elucidation and characterization of the isolated compounds 1–3 were performed using various spectroscopic techniques such as mass, UV, IR, and extensive 1D/2D–NMR spectroscopy. Furthermore, the compounds 1–3 were subjected to screening of anti-cancer activity against different cell lines followed by brief analysis of apoptotic and anti-angiogenic potentials of the potent hit against non-small cell lung carcinoma cell line. All the compounds 1–3 were subjected to anti-proliferative potential against non-small cell lung carcinoma (NCI-H460), breast cancer (MCF-7), cervical cancer (HeLa) and normal mouse fibroblast (NIH-3 T3) cell lines. Among these, compound 3 was found to be more cytotoxic against NCI-H460 and MCF-7 cells (IC50 = 17.86 ± 0.72 and 11.86 ± 0.46 μM respectively). When compared with the standard drug cisplatin compound 3 was found to have more potent activity against NCI-H460 (IC50 = 19 ± 1.24 μM) as compared to MCF-7 cell lines (IC50 = 9.62 ± 0.5 μM). Compound 3 induced apoptosis in NCI-H460 cells in a dose dependent manner. It significantly downregulated, the expression of anti-apoptotic (BCL-2 L1 and p53) and increased the expression of pro-apoptotic (BAK and BAX) genes. Besides apoptosis, it also significantly reduced the cell migration and downregulated the angiogenic genes (i.e. VEGF and COX-2), thereby, inhibiting angiogenesis in NCI-H460 cells. Compound 3 possesses potent anti-proliferative potential as well as induced apoptosis and inhibited the cell migration of the cancerous cells by altering the gene expression, responsible for it.

The phytosociological survey was conducted during 2018-2020. The research area was classified into five ecological zones based on habitat, physiognomy and species composition. Pc-Ord software was used for cluster analysis and four vegetation communities were established. The Quercus baloot-Quercus incana community is situated in Sair at an altitude of 1196 (mean ± SE) m altitude with a 14.1 ± 0 slope angle and contains eleven tree species. The Pinus wallichiana- Ailanthus altissima community had a relatively small number of tree species reported in Shakawlie at 1556 (mean ± SE) with a 17.5 ± 0 slope angle. The Pinus wallichiana- Quercus incana community is distributed in Wali Kandao and Mangi Kandao at altitudes of 2030.5 (mean ± SE) m and the slope angle was 19.2 ± 1.4. This community possesses a total of twenty-one tree species and is highly diverse. Similarly, the Populus alba - Platanus orientalis group was present in Banr Pate, with an altitude of 1613 (mean ± SE) m and a 16.3 slope angle. The principal component analysis (PCA) and non-metric multidimensional scaling (NMS) ordination methods were applied to study the relationships between ecological and soil variables with trees species. The NMS ordination of axis 1 was significantly correlated with Sand% (p<0.2), Nitrogen% (p<0.1) and Pb (mg/kg) (r= 0.876751, p<0.05), while the ordination of axis 2 was significantly correlated with Silt% (p<0.2), Sand% (p<0.2), Organic matter% (p<0.2), K (mg/kg) (r=0.882433, p<0.02), Fe (mg/kg)(r=0.614833, p<0.2), Ca (mg/kg) (r=0.721712, p< 0.2) and Zn (mg/kg) (r=0.609545, p<0.2). Similarly, the PCA ordination of axis 1 revealed that it was significantly correlated with phosphorus, calcium and slope angle, while that of axis 2 was significantly correlated with altitude, zinc and manganese.

The current study was designed to elucidate the effect of silicon and silicon nanoparticles on lead-treated wheat seedlings in hydroponic condition. Pb treatments decreased the shoot length, leaf area, shoot fresh weight, shoot dry weight, roots fresh weight and roots dry weight of wheat seedlings. The photosynthetic pigments (chlorophyll “a”, chlorophyll “b”, carotenoids, and total chlorophyll) were also reduced in leaves of wheat seedlings due to Pb stress. On contrary, addition of Si with Pb and addition of SiNPs with Pb successfully overcome the Pb-induced toxicity. The data showed that Si and SiNPs treatments improved the lead-treated wheat seedlings growth and photosynthetic pigments. It is concluded that Pb causes negative effect on wheat seedlings, however; Si and SiNPs protect the wheat seedlings against Pb toxicity. Further, SiNPs are more effectual than Si to ameliorate the Pb toxicity because of its greater availability to seedlings.