Plague has been a threat to human health in Tanzania since 1886. This zoonotic disease has established several endemic foci in the country, posing a risk of outbreaks. This study was conducted to investigate the presence of Yersinia pestis in small mammals in five districts. These districts were selected because of recent (Mbulu), past (40-18 years ago: Lushoto) and historic (>100 years ago: Iringa and Kilolo) human cases of plague. In addition, one region that has not had any reported human cases of plague was included (Morogoro-Mvomero). Blood from 645 captured small mammals was screened for antibodies against the fraction 1 (F1) antigen of Y. pestis using indirect enzyme-linked immunosorbent assay (ELISA) and competitive-blocking ELISA. Specific antibodies against Y. pestis F1 antigens were detected in six (0.93%) animals belonging to Mastomys natalensis. Of these, four animals were captured in the active focus in Mbulu, and two animals were captured from an area with no history of human plague (Morogoro-Mvomero). These results provide evidence of the circulation of Y. pestis in small mammals in Tanzania. Furthermore, evidence of the circulation of Y. pestis in Morogoro-Mvomero highlights the importance of carrying out plague surveillance in areas with no history of human plague, which can help to predict areas where future outbreaks may occur.

Climate change causes organisms, including species that act as parasite reservoirs and vectors, to shift their distribution to higher altitudes, affecting wildlife infestation patterns. We studied how ectoparasite distributions varied with altitude using two rodent species, Montemys delectorum and Rhabdomys dilectus, at different elevations (1500–3500 m). The ectoparasites infesting the two rodent species were influenced by the host sex, species, and temperature. We expected host density to predict parasite infestation patterns, because hosts in higher densities should have more parasites due to increased contact between individuals. However, temperature, not host density, affected ectoparasite distribution. Since temperatures decrease with elevation, parasite prevalences and abundances were lower at higher elevations, highlighting that the cold conditions at higher elevations limit reproduction and development—this shows that higher elevation zones are ideal for conservation. The rodents and ectoparasite species described in this study have been reported as vectors of diseases of medical and veterinary importance, necessitating precautions. Moreover, Mount Meru is a refuge for a number of endemic and threatened species on the IUCN Red List. Thus, the parasitic infection can also be an additional risk to these critical species as well as biodiversity in general. Therefore, our study lays the groundwork for future wildlife disease surveillance and biodiversity conservation management actions. The study found a previously uncharacterized mite species in the Mesostigmata group that was previously known to be a parasite of honeybees. Further investigations may shed light into the role of this mite species on Mount Meru.

There has been a growing interest in implementing technological food systems to enhance the quality and safety of food items. One such example of this endeavor is the study of the applications of natural biopolymers as food stabilizers. An "emulgel," also known as an emulsion gel, is a gel-like substance that contains a dispersion of emulsion. This emulsion can have a continuous or dispersed phase, creating networks throughout the substance and giving it a semi-solid texture. Gels have garnered significant attention in the food industry due to their ability to be easily shaped and structured and their potential to possess desirable physical, chemical, and mechanical properties. They offer greater stability and controlled release capabilities for the encapsulated bioactive compounds. The review demonstrated that different emulgels exhibit varied relationships between their structure, physicochemical properties, unique applications, and outcomes from different preparation methods. Emulgels enhance the yield, sensory characteristics, and nutritional quality of foods while also controlling lipid oxidation and microbiological development. Emulgels find primary utility in the food business for fat replacement and distribution. Viscosity, temperature, pH, water activity, and ionic strength are influential factors that impact the destabilization mechanisms of emulgels. This article thoroughly summarizes the practical use of emulgels in muscle-based foods and explains their impact on the functioning and quality of such edibles. There is a recommendation to promote the utilisation of emulgels as effective colloids and delivery systems in the future. Additionally, it is proposed that a description of potential new applications in the food industry be provided.

Background and aim: African Greater Cane (ACG) rat populations are widely distributed across Africa but the ancestral origin of populations inhabiting different geographical regions is unknown. There is currently no information available on the genetic differentiation of these populations resulting from long-time geographical isolation, environmental and anthropogenic pressures they face in each region. This limits our ability to understand potential speciation processes in AGC and their implications for their conservation and of other small wild mammals exposed to similar ecological conditions in the savannah biomes in Africa. Methods: We analyzed mitochondrial genes targeting the D-loop region of AGC samples from two blocks of the Eastern Arc Mountains in Tanzania (eastern), three agro-ecological zones in Ghana (western) and four sites in South Africa (southern) to characterize the phylogeographical patterns of the species. Results: AGC populations from Tanzania revealed higher haplotype diversity than those from the two other régions. Analysis of variance (AMOVA) revealed higher genetic variations within than between populations in all geographic regions. Demographic history analysis revealed a negative and significant Tajima’s D for a single southern African population as well as close relatedness with the Eastern Africa populations, suggesting a common ancestral origin. Conclusion: This is the first study to compare maternal lineages of AGC populations from eastern, western and southern Africa and provides a basis for future genetic studies of the species, its evolutionary ecology and the conservation of these populations across their range. Conservation implications: Evidence of recent population size expansion underpinned by high genetic diversity observed in the Uluguru urban (Tanzania) Guinea savannah (Ghana) and Kwazulu Natal (South Africa) populations suggest that the AGC populations are not currently threatened, consistent with the current IUCN status. Also, the distinct haplotypes observed in each region suggest that the populations can be managed as meta-populations, thus providing opportunities for potential local game-farming programs.

The domestic chicken (Gallus gallus domesticus) is one of the most widespread livestock species globally, believed to have originated from Southeast Asia and the Indian Subcontinent over the last 10,000 years. Genetic and archaeological evidence supports multiple independent domestication events involving several wild junglefowl species. This review aimed to synthesize multidisciplinary evidence to trace domestic chickens' origin, dispersal, and their impact in Africa. Specifically, it examined the historical, genetic, and cultural pathways of chicken introduction and adaptation on the continent. A comprehensive literature review was conducted using PubMed, Web of Science, and Google Scholar databases. Peer-reviewed archaeological, linguistic, ethnographic, and molecular genetic studies were integrated, emphasizing mitochondrial DNA (mtDNA), microsatellite markers, and archaeological site data. Findings reveal two major introduction waves of chickens into Africa: the first via the Mediterranean route through Egypt during the Ptolemaic period (300 BC), and the second through the Indian Ocean maritime trade networks between the early and mid-1^st millennium AD. Subsequent overland dispersals extended chickens across the Sahara, Horn of Africa, and West Africa. Genetic studies highlight the complex admixture of maternal lineages from Asia, Europe, and the Middle East, while ethnographic and linguistic data reveal regional adaptation and cultural integration of chickens in African societies. In conclusion, chickens have played multifaceted roles in African food security, economy, and tradition. The evidence underlines a need for conservation of African indigenous chickens, which harbor valuable genetic traits crucial for adaptability and resilience. It is recommended that future research prioritizes whole-genome sequencing, regional genetic characterization, and community-based conservation strategies to preserve biodiversity and promote sustainable poultry development in Africa.