Environmental aquatic pollution with antibiotics is a global challenge that affects even pristine mountain environments. Monitoring the concentration of antibiotics in water is critical to water resource management. In this review, we present the sources and degradation routes of antibiotics polluting surface waters, with particular focus on mountain environments and pristine areas. This pollution is strongly related to anthropopressure resulting from intensive tourism. An important aspect of the threat to the environment is water containing antibiotics at sub-inhibitory concentrations, which affects bacterial populations. Antibiotics are ecological factors driving microbial evolution by changing the bacterial community composition, inhibiting or promoting their ecological functions, and enriching and maintaining drug resistance. We paid attention to the stability of antibiotics and their half-lives in water related to biotic and abiotic degradation, which results from the structures of molecules and environmental conditions. Wastewater treatment combined with advanced treatment techniques significantly increase the efficiency of antibiotic removal from wastewater. Modern methods of wastewater treatment are crucial in reducing the supply of antibiotics to aquatic environments and enhancing the possibility of economic and safe reuse of wastewater for technical purposes. We provide a perspective on current research investigating antibiotic emergence in mountain areas and identify knowledge gaps in this field.

Hydrogels, based on natural polymers, such as hyaluronic acid, are gaining an increasing popularity because of their biological activity. The antibacterial effect of ozone is widely known and used, but the instability the gas causes, severely limits its application. Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives. In this study, we obtained an innovative hydrogel, based on hyaluronic acid containing micro/nanocapsules of ozonated olive oil. By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts. We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method. We also performed Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy. The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line. The studies proved that the obtained emulsions were rheologically stable, exhibited an antimicrobial effect and did not show cytotoxicity in the HaCat keratinocyte model.

Frequent occurrence of microbial resistance to biocides makes it necessary to find alternative antimicrobial substances for modern veterinary medicine. The aim of this study was to obtain biodegradable silver nanoparticle-containing (AgNPs) foils synthesized using non-toxic chemicals and evaluation of their activity against bacterial pathogens isolated from oral cavities of cats, dogs and horses. Silver nanoparticle foils were synthesized using sodium alginate, and glucose, maltose and xylose were used as reducing agents. The sizes of AgNPs differed depending on the reducing agent used (xylose < maltose < glucose). Foil without silver nanoparticles was used as control. Bacterial strains were isolated from cats, dogs and horses by swabbing their oral cavities. Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and extended-spectrum beta-lactamase (ESBL) producing E. coli were isolated on selective chromogenic microbiological media. The bactericidal effect of AgNPs foils obtained using non-toxic chemical compounds against E. coli, ESBL, S. aureus and MRSA isolated from oral cavities of selected animals was confirmed in this study. No statistically significant differences were observed between the foils obtained with different reducing agents. Therefore, all types of examined foils proved to be effective against the isolated bacteria.

Nanometals constitute a rapidly growing area of research within nanotechnology. Nanosilver and nanogold exhibit significant antimicrobial, antifungal, antiviral, anti-inflammatory, anti-angiogenic, and anticancer properties. The size and shape of nanoparticles are critical for determining their antimicrobial activity. In this study, silver and gold nanoparticles were synthesized within a hyaluronic acid matrix utilizing distilled water and distilled water treated with low-pressure, low-temperature glow plasma in an environment of air and argon. Electron microscopy, UV-Vis and FTIR spectra, water, and mechanical measurements were conducted to investigate the properties of nanometallic composites. This study also examined their microbiological properties. This study demonstrated that the properties of the composites differed depending on the preparation conditions, encompassing physicochemical and microbiological properties. The application of plasma-treated water under both air and argon had a significant effect on the size and distribution of nanometals. Silver nanoparticles were obtained between the range of 5 to 25 nm, while gold nanoparticles varied between 10 to 35 nm. The results indicate that the conditions under which silver and gold nanoparticles are produced have a significant effect on their mechanical and antibacterial properties.

Exposure to bioaerosols associated with horse stable indoor environment and their health effects on people and horses has recently become of particular interest. Moreover, increasing frequency of recurrent airway disease (RAO) among horses made it necessary to search for the most probable causal agents of this disease and methods of their eradication. The study was conducted in two horse stables in southern Poland (Kraków and Tarnów). Particulate matter (PM_2.5, PM₄, and PM₁₀) concentrations were determined photometrically, the concentration of fungal aerosol was determined by a six-stage impactor, and next generation sequencing (NGS) was used to determine fungal community composition in one of these stables. The highest PM concentrations were observed in Tarnów, but fungal aerosol levels were higher in the Kraków stable. Based on the NGS results, the three most prevalent fungal species were Wallemia sebi, Aspergillus penicillioides, and Epicoccum nigrum, all highly allergenic and potentially involved in the occurrence of RAO in horses. Spores of the detected fungi can penetrate deeply into the respiratory system. Therefore, this study suggests that examinations of particulate matter and fungal aerosol concentrations, along with species composition assessment, should be regularly conducted in horse stables.

This study explored the synthesis and characterization of pectin-based composites containing encapsulated propolis and sea buckthorn oil. Both propolis and sea buckthorn oil are well known for their antioxidant and antimicrobial properties. To mitigate their sensitivity to environmental degradation, these compounds were encapsulated within a pectin matrix. The composites were prepared using an emulsification technique and subsequently for their physicochemical properties via scanning electron microscopy (SEM), ultraviolet–visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC), as well as color and mechanical testing. The results showed that freeze-dried samples exhibited heterogeneous, bubble-like structures containing nanocapsules (800–2000 nm), whereas for the film samples, the capsules were visibly embedded within the matrix. The study shows that this three-component system exhibits synergistic potential. Encapsulation significantly improved the UV barrier properties and the antioxidant activity of the nanocomposites, which demonstrated greater antioxidant capacity. Microbiological assays revealed that the pectin-based composites containing encapsulated propolis and sea buckthorn oil exhibited strong antibacterial activity, particularly against Gram-positive bacteria such as Streptococcus and Staphylococcus spp. The composites also demonstrated hydrophobic surface characteristics and reduced crystallinity, which correlates with their potential for controlled release. These results underscore the applicability of pectin–propolis–sea buckthorn oil composites as effective natural preservatives or functional ingredients in food systems, due to their high antioxidant and antimicrobial efficacy.