Gut microbiota plays a major role in the process of food absorption and low grade inflammation, two key steps in obesity and diabetes mellitus occurrence. Gut microbiota metabolites, such as short chain fatty acids (SCFA), have an important impact over the metabolic pathways like insulin signalling, incretin production and inflammation. [1-3] We aimed to study the microbiota patterns in obese and T2D patients from Black Sea Coast region, considering the ethnic mixture, environmental and geographical particularities, involving diet or various habits in this area. 100 patients and 100 controls matched by age, gender and ethnicity were studied regarding feaces predominance of Lactobacillus and Bifidobacterium species. We compared the results of microbiota patterns from patients to those obtained in a similar control group of healthy subjects. The standard pour plate 0.05% L-cystine enriched method was used to obtain the bacterial cultures and anaerobic conditions. Morphological and biochemical tests were used to identify the Lactobacillus and Bifidobacterium spp. Fecal organic acid concentrations were explored in frozen samples. The association between bacterial counts/organic acid concentrations and independent variables, including age, diet, ethnicity and other risk factors were calculated using multivariable linear regression analysis. Pearson�s correlation coefficients were calculated to detect associations between fecal bacteria counts/organic acid concentrations and laboratory variables (serum biomarkers, body mass index, age, and severity of obesity/T2D according to international scales). Junk and sweet diets, lack of physical activity and familial aggregation of hypercholesterolemia and diabetes were significantly more often present in our T2D/obese patients than in controls. The bacterial counts of the L. acidophilus, L plantarum and L. reuteri subgroups of Lactobacillus sp were significantly lower among patients with T2D and obesity than in controls. The counting of Bifidobacterium spp revealed a higher presence of B. bifidum in controls than in obese or T2D patients. Diet type (junk food and sweets), BMI (]25) and personal history of metabolic disorders were associated with decreased counts of L acidophilus and increased counts of L. fermentum and B. adolescentis in T2D patients. Ethnicity, metabolic disorders history and junk and sweet diet were associated with low counts of L. acidophilus and L. reuteri and low counts of B. longum. Junk and sweet diet was associated with low counts of B. bifidum. Romanian ethnicity and metabolic disorders were associated with low counts of B. choerinum at obese patients, independent of age or previous antidiabetic treatments. The concentrations of acetic and butyric acids were significantly lower in all patients groups, while the concentrations of valeric acid were significantly higher in patients with untreated T2D and obese patients compared to the controls. Low counts of L. acidophilus and L. reuteri were positively correlated with the increased levels of HbA1c, LDL cholesterol, TG and inflammatory markers such as CRP, ESR and IL-6, no matter of diet, age, ethnicity or metabolic disorders history. Also, low counts of B. bifidum and B. infantis were positively correlated with high levels of CRP, IL-6 and TG. In obese patients, statistic analysis results showed that low counts of L. acidophilus, L. plantarum, L. johnsonii and L. reuteri were positively associated with increased levels of CPR, IL-6 and TG, while low counts of B. bifidum, B infantis and B. breve were positively correlated with higher counts of CPR, LDL cholesterol and TG. Low counts of B. bifidum and B choerinum were positively correlated with low counts of HDL cholesterol in Romanian ethnicity patients and in those with previous metabolic disorders. Low bacterial counts of some particular strains of Lactobacillus spp and Bifidobacterium spp were positively correlated with diet type, BMI, Romanian ethnicity and personal history of metabolic disorders obese and T2D patients from Romanian Black Sea Coast Region.

The delayed healing and infection risks associated with chronic wounds and burns pose significant clinical challenges. Traditional dressings provide basic coverage but lack the bioactive properties needed for tissue regeneration and antimicrobial protection. In this study, we developed zinc alginate hydrogel-coated traditional wound dressings (WD@AlgZn) and evaluated their physicochemical properties, antimicrobial performance, and in vivo healing efficacy. Scanning electron microscopy (SEM) revealed a uniform coating of the zinc alginate network on dressing fibers, while Fourier-transform infrared spectroscopy (FT-IR) confirmed the successful incorporation of zinc ions. Antimicrobial assays further demonstrated that WD@AlgZn reduced bacterial loads (CFU/mL counts) by several orders of magnitude for both Staphylococcus aureus and Escherichia coli compared to uncoated controls. An in vivo rat burn wound model exhibited accelerated wound closure when using WD@AlgZn dressings compared to conventional wound care approaches, achieving a 90.75% healing rate by day 21, significantly outperforming the silver sulfadiazine (52.32%), uncoated-dressing (46.58%), and spontaneous-healing (37.25%) groups. Histological analysis confirmed enhanced re-epithelialization, neovascularization, and reduced inflammation in WD@AlgZn-treated tissues. The findings suggest that WD@AlgZn offers a promising alternative for advanced wound management, combining structural robustness with bioactive properties to support efficient wound healing and infection control. These results provide valuable insights into the potential clinical applications of metal-ion cross-linked biopolymeric hydrogel dressings for next-generation wound care strategies.

Our study, a clinic experiment type, was developed on a sample of 77 patients, aged between 60 and 74 years, taken into study and investigated from an electromyographic point of view, which presented the affliction of muscular activity as a result of decreasing or increasing of muscular tonus, or as a result of muscular contraction alterations. We have applied: occlusal trays (28 days) in all of the patients, aerosols therapy in 28 (36.4%) patients and tolperison hydrochloride (50 mg x 3/day, 28 days) in 27 (35.06%) patients. Also, we have applied occlusal trays and tray to be protective with the composite restoration of patients. The effect of augmentation on the local metabolic processes, the analgesia of painful zones and muscular relaxation have positive effects on long and short term. After applications with aerosols, we recorded amelioration in all patients: the absence of pain and the substantial amelioration of pain perception. Conclusions. Physical kinetic therapeutic methods aim at muscular relaxation, the toning up or the decrease of muscular tonus, improvement in mandible functionality, improvement in functionality at the level of temporomandibular joints and improvement in local circulation. The tolperison hydrochloride treatment was used as symptomatic treatment for muscular relaxation with positive results.

The objective of this paper was to study the suitability of multi gate ion sensitive field effect transistors (ISFETs) for clinical work with specific requirements in the public health evaluation. The obtained sensors were used in a flow cell assembly provided by Bellhouse Medical Products. As in our previous work [1], the sensors were intended for use in the analysis of biological fluids, where the concentrations of specific ions as sodium, potassium and calcium are of critical importance. The electrochemical characteristics for use in clinical chemistry were established.

Affections of temporomandibular joint (TMJ) can lead to imbalances and disfuntions named temporomandibular desorders (TMDs). Elderly people with TMDs are experiencing more severe phenomena due to instability centric relationship, edentation, hypotonia, and cranio-mandibular malrelations, neurologic and chronic diseases. The aim of this study is to investigate the efficiency of the anti-inflamatory treatment followed by prosthetic, estethic and gnathologic treatment in elderly. This is a prospective study, based on data obtained from 410 elderly people, 74 female and 33 male gender subjects. In the studied group we found a high prevalence of pain (42.99%) and articular affectation (25.23%) which is due both to the grade of edentation and to complications of it, also the muscular affectation, headache, modification of the cranial-mandibular relations, psychological affectation. The prevalence of depressive manifestations was high (35.51%) and after the ibuprofen treatment in association with prosthetic treatment was completed decreased (at 23.36%) in the elderly subjects. In conclusion, the anti-inflamatory treatment followed by prosthetic treatment and also esthetical one determined the improved outcomes of the TMDs in elderly patients, not only in terms of pain and depression but also clinically, meaning at the TMJ function and in all the intraoral and facial as