In this study, the extraction efficiency of natural deep eutectic solvents (NADES) based on choline chloride as a hydrogen bond acceptor (HBA) and five different hydrogen bond donors (HBD; lactic acid, 1,4-butanediol, 1,2-propanediol, fructose and urea) was evaluated for the first time for the isolation of valuable bioactive compounds from Achillea millefolium L. The phytochemical profiles of NADES extracts obtained after ultrasound-assisted extraction were evaluated both spectrophotometrically (total phenolic content (TPC) and antioxidant assays) and chromatographically (UHPLC-MS and HPLC-UV). The results were compared with those obtained with 80% ethanol, 80% methanol, and water. The highest TPC value was found in the lactic acid-based NADES (ChCl-LA), which correlated with the highest antioxidant activity determined by the FRAP analysis. On the other hand, the highest antiradical potential against ABTS^+• was determined for urea-based NADES. Phenolic acids (chlorogenic acid and dicaffeoylquinic acid isomers), flavones (luteolin and apigenin), and their corresponding glucosides were determined as the dominant individual phenolic compounds in all extracts. The antibacterial and antifungal properties of the extracts obtained against four bacterial cultures and two yeasts were evaluated using two methods: the agar dilution method to obtain the minimum inhibitory concentration (MIC) and the minimum bactericidal or fungicidal concentration (MBC or MFC), and the disc diffusion method. ChCl-LA had the lowest MIC and MBC/MFC with respect to all microorganisms, with an MIC ranging from 0.05 mg mL⁻¹ to 0.8 mg mL⁻¹, while the water extract had the weakest inhibitory activity with MIC and MBC/MFC higher than 3.2 mg mL⁻¹.

This work presents the advantages of using ultrasound (US) in the procedure of antimicrobial functionalization of cotton knitwear. Ultrasound-assisted extraction of bioactive components from plant material reduced the extraction time by 40% and increased the degree of utilization by 12.5% in comparison with conventional extraction. By optimizing the H₂O₂ bleaching process, it was found that the application of ultrasound resulted in reducing the temperature from 100 °C to 60 °C, the processing time by 30 min and the H₂O₂ concentration from 4% to 2%, while retaining the same weight loss, the appropriate degree of whiteness with preserved mechanical properties compared with treatment carried out without using ultrasound. Dyeing with the plant extract of Achillea millefolium L. using the ultrasonic atomization resulted in even coloration and antibacterial activity against Staphylococcus aureus and Escherichia coli, with using minimum quantities of extract and 6.67 times shorter processing time in comparison with the bath exhaustion method.

The development of automated software and the device for determination of wicking of textile materials, using open-source ImageJ libraries for image processing, and newly designed additional algorithm for the determination of threshold, is presented in this paper. The description of the device, design of the open-source software “Kapilarko”, as well as an explanation of the steps: image processing, threshold determination and reading of wicking height, are provided. We have also investigated the possibility of using the artificial neural networks for automatic recognition of the wicking height. The results showed that the recognition of the wet area of the sample, based on the application of artificial neural networks was in a very good agreement with the experimental data. The device's utility for the measurement of wicking ability of textile materials was proved by testing various knitted fabrics. The constructed device has the advantages of providing automated measurement and minimization of the subjective errors of the operators; extremely fast or long-term measurements; digital recording of results; consistency of experimental conditions; possibility of using water instead of colors and, last but not least, low cost of the device. Considering the importance and frequent measurements of wicking ability of textile materials, the advantages of the presented device, as well as the fact that commercial software without publishing the source-code, are used for most of the available devices, we believe that our idea to design the automated software and device by applying the "open-source" approach, will be of benefit to scientists and engineers in using or improving wicking experiments.