Metagenomics promises to provide new valuable insights into the role of microbiomes in eukaryotic hosts such as humans. Due to the decreasing costs for sequencing, public and private repositories for human metagenomic datasets are growing fast. Metagenomic datasets can contain terabytes of raw data, which is a challenge for data processing but also an opportunity for advanced machine learning methods like deep learning that require large datasets. However, in contrast to classical machine learning algorithms, the use of deep learning in metagenomics is still an exception. Regardless of the algorithms used, they are usually not applied to raw data but require several preprocessing steps. Performing this preprocessing and the actual analysis in an automated, reproducible, and scalable way is another challenge. This and other challenges can be addressed by adjusting known big data methods and architectures to the needs of microbiome analysis and DNA sequence processing. A conceptual architecture for the use of machine learning and big data on metagenomic data sets was recently presented and initially validated to analyze the rumen microbiome. The same architecture can be used for clinical purposes as is discussed in this paper.

To cope with the growing number of multimedia assets on smartphones and social media, an integrated approach for semantic indexing and retrieval is required. Here, we introduce a generic framework to fuse existing image and video analysis tools and algorithms into a unified semantic annotation, indexing and retrieval model resulting in a multimedia feature vector graph representing various levels of media content, media structures and media features. Utilizing artificial intelligence (AI) and machine learning (ML), these feature representations can provide accurate semantic indexing and retrieval. Here, we provide an overview of the generic multimedia analysis framework (GMAF) and the definition of a multimedia feature vector graph framework (MMFVGF). We also introduce AI4MMRA to detect differences, enhance semantics and refine weights in the feature vector graph. To address particular requirements on smartphones, we introduce an algorithm for fast indexing and retrieval of graph structures. Experiments to prove efficiency, effectiveness and quality of the algorithm are included. All in all, we describe a solution for highly flexible semantic indexing and retrieval that offers unique potential for applications such as social media or local applications on smartphones.

The indexing and retrieval of multimedia content is generally implemented by employing feature graphs. These graphs typically contain a significant number of nodes and edges to reflect the level of detail in feature detection. A higher level of detail increases the effectiveness of the results, but also leads to more complex graph structures. However, graph traversal-based algorithms for similarity are quite inefficient and computationally expensive, especially for large data structures. To deliver fast and effective retrieval especially for large multimedia collections and multimedia big data, an efficient similarity algorithm for large graphs in particular is desirable. Hence, in this paper, we define a graph projection into a 2D space (Graph Code) and the corresponding algorithms for indexing and retrieval. We show that calculations in this space can be performed more efficiently than graph traversals due to the simpler processing model and the high level of parallelization. As a consequence, we demonstrate experimentally that the effectiveness of retrieval also increases substantially, as the Graph Code facilitates more levels of detail in feature fusion. These levels of detail also support an increased trust prediction, particularly for fused social media content. In our mathematical model, we define a metric triple for the Graph Code, which also enhances the ranked result representations. Thus, Graph Codes provide a significant increase in efficiency and effectiveness, especially for multimedia indexing and retrieval, and can be applied to images, videos, text and social media information.

Genetics plays an ever-increasing role in medical diagnostics. The requirements for laboratory diagnostics are constantly changing due to new emerging diagnostic procedures, methodologies, devices, and regulatory requirements. Standard software already available for laboratories often cannot keep up with the latest developments or is focused on research rather than process automation. Although the software utilized in diagnostic laboratories is subject to regulatory requirements, there is no well-defined formal procedure for software development. Reference models have been developed to formalize these solutions, but they do not facilitate the initial requirements analysis or the development process itself. A systematic requirements engineering process is however not only essential to ensure the quality of the final product but is also required by regulations such as the European In Vitro Diagnostic Regulation and international standards such as IEC 62304. This paper shows, by example, the systematic requirements analysis of a system for qPCR-based (quantitative polymerase chain reaction) gene expression analysis. Towards this goal, a multi-step research approach was employed, which included literature review, user interviews, and market analysis. Results revealed the complexity of the field with many requirements to be considered for future implementation.