The aim of this work was to evaluate the cytotoxicity of Arthrospira platensis Extracellular Polymeric Substances (EPS) for colon cancer and kidney cell lines. Results showed that EPS were free from cytotoxic effects. A variety of solvents were assessed for their ability to extract the bioactive ingredients from EPS. Methanol gave the highest yield (75.75%) than other solvents. The extracts were tested for activities against a collection of Gram+/- bacteria. The methanol extract exhibited a more potent activity than the other organic extracts, whereas the aqueous extract was active against Staphylococcus epidermis (Gram+) and Salmonella typhimurium (Gram-). Finally, The extracts were also tested for the antioxidant activity, using the Trolox Equivalent Antioxidant Activity assay. The methanol extract displayed a moderate antioxidant activity (TEAC = 0.027 mg/ml). The HPLC analysis of this extract revealed two distinct peaks: 8.1 kDa (8.31 min) and 4.1 kDa (8.54 min).