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Spain has more than 2.5 M ha of olive groves, with 60% of this area (i.e., 1.5 M ha) concentrated in the region of Andalusia (Southern Spain). Assuming the socio-ecological characteristics of these crops, of which their contribution to ecosystemic services (ES) is fundamental for society, it is highly relevant to direct their management towards practices that guarantee their durability. Organic management of olive groves constitutes a multifunctional model that contributes to ensuring its sustainability and represents 2.4–3.5% of the olive grove area in Spain. Taking the Protected Designation of Origin (PDO) Estepa (Southwestern Spain) as a study model, where organic olive groves are novel, a study of the impacts of erosion on the economic, social, and environmental factors associated with this management was carried out in addition to estimating its impacts. The results showed how organic management promotes edaphic fertility, keeping the levels of diffuse pollution under the legislative limits. Although the increase in erosion has negative effects on the sustainability/durability of agricultural holdings, organic management consolidates a sustainable model that satisfies farmers’ demands. Therefore, organic farming is a model that focuses on the correct use of natural resources associated with the geographical region of study, and contributes to increasing the sustainability of olive groves.
Antonio Rodríguez Sousa; Carlos Parra-López; Samir Sayadi-Gmada; Jesús Barandica; Alejandro Rescia. Impacts of Erosion on the Sustainability of Organic Olive Groves: A Case Study (Estepa Region, Southwestern Spain). Sustainability 2021, 13, 7983 .
AMA StyleAntonio Rodríguez Sousa, Carlos Parra-López, Samir Sayadi-Gmada, Jesús Barandica, Alejandro Rescia. Impacts of Erosion on the Sustainability of Organic Olive Groves: A Case Study (Estepa Region, Southwestern Spain). Sustainability. 2021; 13 (14):7983.
Chicago/Turabian StyleAntonio Rodríguez Sousa; Carlos Parra-López; Samir Sayadi-Gmada; Jesús Barandica; Alejandro Rescia. 2021. "Impacts of Erosion on the Sustainability of Organic Olive Groves: A Case Study (Estepa Region, Southwestern Spain)." Sustainability 13, no. 14: 7983.
During the tanning step, 30–40% of trivalent chromium remains in the solid and liquid wastes, generating highly polluted sewage. In this study, powdered marble was used as a low-cost adsorbent to remove trivalent chromium from a synthetic solution and a real tanning effluent. Batch adsorption experiments on powdered marble were performed. After incubation, samples were filtered and the uptake of chromium was determined. A 3-level Box-Behnken Design was used to study the combined effect of initial chromium concentration, pH values and adsorbent dose on the removal of trivalent chromium. The results showed that the maximum uptake (> 99%) was obtained at a chromium initial concentration of 0.758 g/L, pH = 5.0 and using 6% (w:v) of powdered marble. Adsorption isotherm was satisfactorily described by Langmuir model. The toxicity of the tanning effluent was examined before and after adsorption. Phytotoxicity (Lepidium sativum) assays revealed an improvement of the germination index rising from 0 to 35% before and after adsorption, respectively. Microtoxicity (Escherichia coli) assays showed a decrease in the growth inhibition percentage going from 100 to 31% following the adsorption experiment using raw tanning effluent. Desorption study indicated that nearly 45% of the adsorbed trivalent chromium was recovered using 4% (v:v) HCl. Overall, results showed that powdered marble is an effective and an economical alternative with great potential for trivalent chromium recovery.
R. Boujelben; M. Ellouze; F. Aziz; N. Ouazzani; S. Sayadi. Box-Behnken approach for optimization of Cr(III) removal from a real tanning effluent using powdered marble. International Journal of Environmental Science and Technology 2021, 1 -16.
AMA StyleR. Boujelben, M. Ellouze, F. Aziz, N. Ouazzani, S. Sayadi. Box-Behnken approach for optimization of Cr(III) removal from a real tanning effluent using powdered marble. International Journal of Environmental Science and Technology. 2021; ():1-16.
Chicago/Turabian StyleR. Boujelben; M. Ellouze; F. Aziz; N. Ouazzani; S. Sayadi. 2021. "Box-Behnken approach for optimization of Cr(III) removal from a real tanning effluent using powdered marble." International Journal of Environmental Science and Technology , no. : 1-16.
Despite the growing importance of the digital transformation (DT) of the agrifood sector on the political agenda, traditional policies are not enough to provide proactive responses to rapid technological changes and new approaches for policy planning are necessary especially at regional level. This manuscript proposes and illustrates the implementation of a new methodological framework for DT policy planning in the case of Andalusia, the olive world leader region, but applicable to other regions and sectors, with two objectives: 1) to quantitatively determine the importance of the conditioning factors of DT in the olive sector in the short/medium term, by developing an AHP/SWOT/PESTLE model, and 2) to design public policies to strengthen the DT, taking advantage of the potentialities and alleviating the deficiencies, by carrying out a quantitative TOWS analysis. The knowledge of diverse groups of experts, i.e. stakeholders in the sector, has been used in all analyses due to the lack of reliable data and the complex nature of the issues analysed. The results show that the opportunities and strengths are more prominent than weaknesses and threats for DT. Environmental issues stand out as an opportunity to boost DT. There is also a growing interest in developing an interoperability strategy which is an opportunity to overcome the low technological integration of the value chain. DT can also enable a more transparent value chain and improved traceability. Some negative factors are the lack of evidence on the economic viability of investment in digital technologies, shortage of labour and young farmers, and potential unintended and unanticipated effects of DT. Important policies strategies to foster DT are: improving environmental efficiency though DT; promoting youth employment in the sector; enhancing coordination among innovation actors; developing a common interoperability strategy; and fostering technological integration in the sector.
Carlos Parra-López; Liliana Reina-Usuga; Carmen Carmona-Torres; Samir Sayadi; Laurens Klerkx. Digital transformation of the agrifood system: Quantifying the conditioning factors to inform policy planning in the olive sector. Land Use Policy 2021, 108, 105537 .
AMA StyleCarlos Parra-López, Liliana Reina-Usuga, Carmen Carmona-Torres, Samir Sayadi, Laurens Klerkx. Digital transformation of the agrifood system: Quantifying the conditioning factors to inform policy planning in the olive sector. Land Use Policy. 2021; 108 ():105537.
Chicago/Turabian StyleCarlos Parra-López; Liliana Reina-Usuga; Carmen Carmona-Torres; Samir Sayadi; Laurens Klerkx. 2021. "Digital transformation of the agrifood system: Quantifying the conditioning factors to inform policy planning in the olive sector." Land Use Policy 108, no. : 105537.
Agriculture has played an essential role in the provision of food and has been a major factor in overall economic development for societies around the world for millennia. In the past, agriculture in hot, arid countries like Qatar faced many challenges, the primary one being a dearth of water for irrigation. Historically this severely limited Qatar’s economic development, which was based largely on resource exploitation, pearl fishing, and only more recently, on the exploitation of its oil and gas reserves which subsequently has led to Qatar’s great wealth. This paper gives an overview of the recent evolution of Qatar’s agricultural sector and investigates future trends that tackle the challenges of its hot arid climate and the limited availability of agricultural resources. Specifically, the review analyses Qatar’s potential to develop a national food security strategy based on a significant expansion of food production in the country. We review recent policy actions implemented to address challenges in the food supply chain caused by a 3.5-year blockade imposed by the adjacent Arab Gulf States, discussing the renewed interest in the potential that an enhanced agricultural sector must provide some aspects of food security and the implications for policymakers that would logically ensue.
Theodora Karanisa; Alexandre Amato; Renee Richer; Sara Abdul Majid; Cynthia Skelhorn; Sami Sayadi. Agricultural Production in Qatar’s Hot Arid Climate. Sustainability 2021, 13, 4059 .
AMA StyleTheodora Karanisa, Alexandre Amato, Renee Richer, Sara Abdul Majid, Cynthia Skelhorn, Sami Sayadi. Agricultural Production in Qatar’s Hot Arid Climate. Sustainability. 2021; 13 (7):4059.
Chicago/Turabian StyleTheodora Karanisa; Alexandre Amato; Renee Richer; Sara Abdul Majid; Cynthia Skelhorn; Sami Sayadi. 2021. "Agricultural Production in Qatar’s Hot Arid Climate." Sustainability 13, no. 7: 4059.
A closed-vessel microwave-assisted extraction (MAE) of simmondsins and polyphenols from defatted Jojoba cake using Box-Benkhen design with four independent variables (solvent/cake ratio, ethanol concentration, extraction time and microwave power) was investigated. ANOVA results showed that the obtained models were significant at 95 % confidence level. Optimal extraction conditions were found for highest values of microwave power (500 W) and extraction time (15 min) and for moderate values of solvent to cake ratio (41 – 45 mL/g). Optimum simmondsins yield (23.35 %) was obtained with pure water as solvent. However, optimum polyphenols yield (2.33 %) and ORAC antioxidant activity (656 µmol TE/g) were obtained with 46.79 % and 42.04 % ethanol in water, respectively. ORAC antioxidant activity was found to be well correlated to polyphenol and simmondsin contents. These results indicate that MAE is an effective technique for recovery of bioactive compounds for food and pharmaceutical industries from Jojoba by-products.
Firas Feki; Daniela Klisurova; Mohamed Ali Masmoudi; Sirine Choura; Petko Denev; Antoaneta Trendafilova; Mohamed Chamkha; Sami Sayadi. Optimization of microwave assisted extraction of simmondsins and polyphenols from Jojoba (Simmondsia chinensis) seed cake using Box-Behnken statistical design. Food Chemistry 2021, 356, 129670 .
AMA StyleFiras Feki, Daniela Klisurova, Mohamed Ali Masmoudi, Sirine Choura, Petko Denev, Antoaneta Trendafilova, Mohamed Chamkha, Sami Sayadi. Optimization of microwave assisted extraction of simmondsins and polyphenols from Jojoba (Simmondsia chinensis) seed cake using Box-Behnken statistical design. Food Chemistry. 2021; 356 ():129670.
Chicago/Turabian StyleFiras Feki; Daniela Klisurova; Mohamed Ali Masmoudi; Sirine Choura; Petko Denev; Antoaneta Trendafilova; Mohamed Chamkha; Sami Sayadi. 2021. "Optimization of microwave assisted extraction of simmondsins and polyphenols from Jojoba (Simmondsia chinensis) seed cake using Box-Behnken statistical design." Food Chemistry 356, no. : 129670.
Background The three‐phase extraction process of olive oil produces highly contaminated wastewater (OMW). The elimination of this toxic by‐product is an important environmental issue that requires the development of an appropriate management solution. Therefore, microalgae cultivation using OMW as growth medium was studied using single (the culture medium was formed by 0 to 80% OMUF or OMW added to BG11) and two‐stage strategies (microalgae were firstly cultivated in the BG11 medium. In the second stage, 40 and 80% of OMUF and OMW were added to the culture). In this work, biodegradation of OMW and subsequent extraction of lipid and antioxidant molecules were investigated as an ecofriendly method for the bioremediation and valorization of OMW. Results It was seen that for two‐stage cultivation, OMUF and OMW stress enhanced the intracellular amount of polyphenol accumulated in Scenedesmus sp. and exhibited the highest DPPH and ABTS scavenging ability compared to single‐stage cultivation. Moreover, the lipid profile is dominated by polyunsaturated acids. In the single‐stage cultivation, the Ch a, Ch b, carotenoid, carbohydrate and lipid contents of 2.57, 7.4, 1.69, 368 and 644 g kg−1 were observed in 40% OMUF added culture, respectively, along with high biomass productivity and 58% of polyphenol removal. Moreover, the MTT assay showed that the biomass of Scenedesmus sp. cultured on 40% OMUF did not show any toxic effect, making it an efficient strategy. Conclusion The obtained results indicate that Scenedesmus sp. is a promising microalga for the biotreatment of OMW and the extraction of bioactive metabolites. This article is protected by copyright. All rights reserved.
Ines Dahmen‐Ben Moussa; Amina Maalej; Mohamed Ali Masmoudi; Firas Feki; Sirine Choura; Nidhal Baccar; Lobna Jelail; Fatma Karray; Mohamed Chamkha; Sami Sayadi. Effect of olive mill wastewaters on Scenedesmus sp. growth, metabolism and polyphenols removal. Journal of the Science of Food and Agriculture 2021, 1 .
AMA StyleInes Dahmen‐Ben Moussa, Amina Maalej, Mohamed Ali Masmoudi, Firas Feki, Sirine Choura, Nidhal Baccar, Lobna Jelail, Fatma Karray, Mohamed Chamkha, Sami Sayadi. Effect of olive mill wastewaters on Scenedesmus sp. growth, metabolism and polyphenols removal. Journal of the Science of Food and Agriculture. 2021; ():1.
Chicago/Turabian StyleInes Dahmen‐Ben Moussa; Amina Maalej; Mohamed Ali Masmoudi; Firas Feki; Sirine Choura; Nidhal Baccar; Lobna Jelail; Fatma Karray; Mohamed Chamkha; Sami Sayadi. 2021. "Effect of olive mill wastewaters on Scenedesmus sp. growth, metabolism and polyphenols removal." Journal of the Science of Food and Agriculture , no. : 1.
A halotolerant marine strain PHKT of Halomonas venusta was isolated from contaminated seawater as an efficient biosurfactant producer candidate, on low-value substrate (glycerol). The produced biosurfactants (Bios-PHKT) were characterized as lipopeptides molecules, belonging to surfactin and pumilacidin families, by using Thin Layer Chromatography (TLC), Fourier Transform Infrared Spectroscopy (FT-IR) and Tandem Mass Spectrometry (MALDI-TOF/MS-MS). Bios-PHKT has a critical micelle concentration (CMC) of 125 mg/L, and showed a high steadiness against a wide spectrum of salinity (0–120 g/L NaCl), temperature (4–121 °C) and pH (2–12), supporting its powerful tensioactive properties under various environmental conditions. Likewise, the cytotoxic test revealed that the biosurfactant Bios-PHKT, at concentrations lower than 125 µg/mL, was not cytotoxic for human HEK-293 cells since the cell survival is over than 80%. Furthermore, Bios-PHKT lipopeptides showed excellent anti-adhesive and anti-biofilm activities, being able to avoid and disrupt the biofilm formation by certain pathogenic microorganisms. In addition, the biosurfactant Bios-PHKT showed a remarkable anti-proliferative activity towards tumor B16 melanoma cell line. Besides, Bios-PHKT exhibited an excellent in vitro and in vivo wound healing process. In light of these promising findings, Bios-PHKT could be successfully used in different biotechnological applications.
Meriam Cheffi; Amina Maalej; Asma Mahmoudi; Dorra Hentati; Ana Maria Marques; Sami Sayadi; Mohamed Chamkha. Lipopeptides production by a newly Halomonas venusta strain: Characterization and biotechnological properties. Bioorganic Chemistry 2021, 109, 104724 .
AMA StyleMeriam Cheffi, Amina Maalej, Asma Mahmoudi, Dorra Hentati, Ana Maria Marques, Sami Sayadi, Mohamed Chamkha. Lipopeptides production by a newly Halomonas venusta strain: Characterization and biotechnological properties. Bioorganic Chemistry. 2021; 109 ():104724.
Chicago/Turabian StyleMeriam Cheffi; Amina Maalej; Asma Mahmoudi; Dorra Hentati; Ana Maria Marques; Sami Sayadi; Mohamed Chamkha. 2021. "Lipopeptides production by a newly Halomonas venusta strain: Characterization and biotechnological properties." Bioorganic Chemistry 109, no. : 104724.
The effects of aridity on soil and water-use efficient (WUE) crop species are relatively well known. However, the understanding of its impacts on the dynamics of below-ground microorganisms associated with plant roots is less well understood. To investigate the influence of increasing aridity on the dynamics of the fungal communities, samples from the root endosphere and rhizosphere associated with the prickly pear cactus trees (Opuntia ficus-indica) growing along the aridity gradient were collected and the internal transcribed spacer (ITS) were sequenced. The diversity and network analyses of fungal taxa were determined along with standard measurements of soil parameters. We found that (i) the fungal community exhibited similar alpha diversity and shared a set of core taxa within the rhizosphere and endosphere, but there was significant beta diversity differences; (ii) the relative abundance of major phyla was higher in the rhizosphere than in the endosphere; (iii) arbuscular endomycorrhizal colonization was highest in the humid climate and decreased under lower-arid, and was negatively correlated with increased concentration of Ca2+ in the soil; (iv) increased aridity correlated with increased connectivity of the soil microbial-root fungal networks in the arid soils, producing a highly cohesive network in the upper-arid area; and (v) distinct fungal hubs sculpt the fungal microbiome network structure in the rhizosphere and endosphere within each bioclimatic zone. Our findings highlight the importance of gradient analysis-based correlation network as a powerful approach to understand changes in the diversity, the dynamics, and the structure of fungal communities associated with the rhizosphere-endosphere interaction and led to the identification of microbes at each bioclimatic zone that are potentially involved in promoting the survival, protection, and growth of Opuntia trees. The variability of fungal hubs composition depending on plant compartment and bioclimatic zone will give key implications for the application of rhizospheric fungi and endophytes as microbial inoculants in agriculture, as well as in the conservation and restoration of cacti plants in arid and semi-arid lands against the backdrop of climate change. Overall, this study will enhance our understanding of the microbiomes'dynamic of CAM plants in nature.
Mahmoud Gargouri; Fatma Karray; Asma Chebaane; Najla Mhiri; Laila Pamela Partida-Martínez; Sami Sayadi; Ahmed Mliki. Increasing aridity shapes beta diversity and the network dynamics of the belowground fungal microbiome associated with Opuntia ficus-indica. Science of The Total Environment 2021, 773, 145008 .
AMA StyleMahmoud Gargouri, Fatma Karray, Asma Chebaane, Najla Mhiri, Laila Pamela Partida-Martínez, Sami Sayadi, Ahmed Mliki. Increasing aridity shapes beta diversity and the network dynamics of the belowground fungal microbiome associated with Opuntia ficus-indica. Science of The Total Environment. 2021; 773 ():145008.
Chicago/Turabian StyleMahmoud Gargouri; Fatma Karray; Asma Chebaane; Najla Mhiri; Laila Pamela Partida-Martínez; Sami Sayadi; Ahmed Mliki. 2021. "Increasing aridity shapes beta diversity and the network dynamics of the belowground fungal microbiome associated with Opuntia ficus-indica." Science of The Total Environment 773, no. : 145008.
Microbial production of bioplastics, derived from poly(3-hydroxybutyrate) (PHB), have provided a promising alternative towards plastic pollution. Compared to other extremophiles, halophilic archaea are considered as cell factories for PHB production by using renewable, inexpensive carbon sources, thus decreasing the fermentation cost. This study is aimed at screening 33 halophilic archaea isolated from three enrichment cultures from Tunisian hypersaline lake, Chott El Jerid, using starch as the sole carbon source by Nile Red/Sudan Black staining and further confirmed by PCR amplification of phaC and phaE polymerase genes. 14 isolates have been recognized as positive candidates for PHA production and detected during both seasons. The identification of these strains through 16S rRNA gene analyses showed their affiliation to Halorubrum, Natrinema, and Haloarcula genera. Among them, three PHB-producing strains, CEJ34-14, CEJ5-14, and CEJ48-10, related to Halorubrum chaoviator, Natrinema pallidum, and Haloarcula tradensis were found to be the best ones reaching values of 9.25, 7.11, and 1.42% of cell dry weight (CDW), respectively. Our findings highlighted that Halorubrum, Natrinema, and Haloarcula genera were promising candidates for PHB production using soluble starch as a carbon source under high salinity (250 g L-1 NaCl).
Fatma Karray; Manel Ben Abdallah; Nidhal Baccar; Hatem Zaghden; Sami Sayadi. Production of Poly(3-Hydroxybutyrate) by Haloarcula, Halorubrum, and Natrinema Haloarchaeal Genera Using Starch as a Carbon Source. Archaea 2021, 2021, 1 -10.
AMA StyleFatma Karray, Manel Ben Abdallah, Nidhal Baccar, Hatem Zaghden, Sami Sayadi. Production of Poly(3-Hydroxybutyrate) by Haloarcula, Halorubrum, and Natrinema Haloarchaeal Genera Using Starch as a Carbon Source. Archaea. 2021; 2021 ():1-10.
Chicago/Turabian StyleFatma Karray; Manel Ben Abdallah; Nidhal Baccar; Hatem Zaghden; Sami Sayadi. 2021. "Production of Poly(3-Hydroxybutyrate) by Haloarcula, Halorubrum, and Natrinema Haloarchaeal Genera Using Starch as a Carbon Source." Archaea 2021, no. : 1-10.
The hydro-distilled leaves of Pistacia lentiscus are considered as an agricultural residue and of no commercial value yet. In this study, chromatographic purification of a methanolic fraction of P. lentiscus L. (lentisk) distilled leaves yielded pure compounds, including Quercitrin (QUE) and Loliolid (LOL) which are characterized, for the first time, in the lentisk leaves’ residue. The inhibitory potential of QUE and LOL was investigated against tyrosinase and acetylcholinesterase activities using enzymatic assays and lysozyme fibrillation through Thioflavin-T fluorescence assay. Their binding mode to Bovine Serum Albumin (BSA) was also assessed by several spectroscopic analyses. Results show that QUE was more potent to inhibit enzyme activity than LOL. Besides, the Thioflavin-T assay confirmed that only QUE was able to block the fibril formation at a concentration of 25 μg/mL. Furthermore, the BSA quenching mechanism by QUE and LOL is a static process with conformational changes in BSA. Interestingly, our findings may provide novel insights into the potentiality of lentisk to be used as a bioresource for the generation of Quercitrin and Loliolid which can be commercially exploited in the field of cosmeceuticals and nutraceuticals.
Amina Maalej; Wiem Elloumi; Ivan Angelov; Proletina Kardaleva; Vladimir Dimitrov; Mohamed Chamkha; Maya Guncheva; Sami Sayadi. Pistacia lentiscus by-product as a promising source of phenolic compounds and carotenoids: Purification, biological potential and binding properties. Food and Bioproducts Processing 2021, 126, 245 -255.
AMA StyleAmina Maalej, Wiem Elloumi, Ivan Angelov, Proletina Kardaleva, Vladimir Dimitrov, Mohamed Chamkha, Maya Guncheva, Sami Sayadi. Pistacia lentiscus by-product as a promising source of phenolic compounds and carotenoids: Purification, biological potential and binding properties. Food and Bioproducts Processing. 2021; 126 ():245-255.
Chicago/Turabian StyleAmina Maalej; Wiem Elloumi; Ivan Angelov; Proletina Kardaleva; Vladimir Dimitrov; Mohamed Chamkha; Maya Guncheva; Sami Sayadi. 2021. "Pistacia lentiscus by-product as a promising source of phenolic compounds and carotenoids: Purification, biological potential and binding properties." Food and Bioproducts Processing 126, no. : 245-255.
Anaerobic digestion is an environmentally sustainable technology for converting a variety of organic solid feedstocks to energy in the form of methane. To improve methane yield, the digestion of more than one waste is commonly applied. This study aimed to investigate the dry anaerobic co-digestion (TS of 20%) of vegetable wastes (VW) and animal manures (cow manure (CM) and poultry manure (PM)) through conducting biochemical methane potential (BMP) assays at different substrate to inoculum (S/I) ratio. Fractionation and fluorescence analyses showed that organic matter of VW was less accessible and less complex compared to that of CM and PM. The highest methane yields of mono-digestion experiments were registered with S/I 0.5. Best results of methane yield were observed by the co-digestion of VW and CM (284 mL/g VS), which was about 2.3-fold higher than that of VW mono-fermentation. This finding was confirmed at a laboratory-scale leach bed reactor. Result showed an increase of biogas yield from 220 to 445 mL/g VS, during mono and co-digestion, respectively. The co-digestion of VW and CM mixture led to the enhancement of both methane yield and digestate quality in comparison to mono-digestion.
Najwa Mlaik; Sami Sayadi; Nesrine Mnasri; Sonia Kechaou; Salim Loukil; Fathi Aloui; Sonia Khoufi. Dry mesophilic anaerobic co-digestion of vegetable wastes with animal manures using leach bed reactor. Biomass Conversion and Biorefinery 2021, 1 -11.
AMA StyleNajwa Mlaik, Sami Sayadi, Nesrine Mnasri, Sonia Kechaou, Salim Loukil, Fathi Aloui, Sonia Khoufi. Dry mesophilic anaerobic co-digestion of vegetable wastes with animal manures using leach bed reactor. Biomass Conversion and Biorefinery. 2021; ():1-11.
Chicago/Turabian StyleNajwa Mlaik; Sami Sayadi; Nesrine Mnasri; Sonia Kechaou; Salim Loukil; Fathi Aloui; Sonia Khoufi. 2021. "Dry mesophilic anaerobic co-digestion of vegetable wastes with animal manures using leach bed reactor." Biomass Conversion and Biorefinery , no. : 1-11.
The Chlorophyta Picocystis sp. isolated from a Tunisian household sewage pond appears promising for effective removal of Bisphenol A (BPA). Efficient and cost-effective technology for contaminants remediation relies on a tradeoff between several parameters such as removal efficiency, microorganism growth, and its tolerance to contaminant toxicity. This article demonstrates the optimum conditions achieving the highest removal rates and the minimal growth inhibition in batch cultures of Picocystis using response surface methodology. A central composite face-centered (CCF) design was used to determine the effects on removal and growth inhibition of four operating parameters: temperature, inoculum cell density, light intensity, and initial BPA concentration. Results showed that the maximal BPA removal was 91.36%, reached the optimal culture conditions of 30.7 °C, 25 × 105 cells ml−1 inoculum density, 80.6 µmol photons m−2 s−1 light intensity, and initial BPA concentration of 10 mg l−1. Various substrate inhibition models were used to fit the experimental data, and robustness analysis highlighted the Tessier model as more efficient to account for the interaction between Picocystis and BPA and predict removal efficiency. These results revealed how Picocystis respond to BPA contamination and suggest that optimization of experimental conditions can be effectively used to maximize BPA removal in the treatment process.
Rihab Ben Ali; Sabrine Ben Ouada; Christophe Leboulanger; Jihene Ammar; Sami Sayadi; Hatem Ben Ouada. Bisphenol A removal by the Chlorophyta Picocystis sp.: optimization and kinetic study. International Journal of Phytoremediation 2020, 1 -11.
AMA StyleRihab Ben Ali, Sabrine Ben Ouada, Christophe Leboulanger, Jihene Ammar, Sami Sayadi, Hatem Ben Ouada. Bisphenol A removal by the Chlorophyta Picocystis sp.: optimization and kinetic study. International Journal of Phytoremediation. 2020; ():1-11.
Chicago/Turabian StyleRihab Ben Ali; Sabrine Ben Ouada; Christophe Leboulanger; Jihene Ammar; Sami Sayadi; Hatem Ben Ouada. 2020. "Bisphenol A removal by the Chlorophyta Picocystis sp.: optimization and kinetic study." International Journal of Phytoremediation , no. : 1-11.
A strain NAPH6 of Pseudomonas aeruginosa, isolated from contaminated seawater taken from the fishing harbor of Sfax, Tunisia, was able to degrade almost the totality of naphthalene (200 mg/l) and the aliphatic hydrocarbons of crude oil (1%, v/v), after 7 and 20 days of culture, respectively, at 30 g/l NaCl. Moreover, the isolate NAPH6 exhibited a notable potentiality to synthesize biological surfactants (BS-NAPH6) on inexpensive carbon sources. The Fourier Transform Infrared (FTIR) analysis suggested that BS-NAPH6 belonged to the glycolipid family. Besides, BS-NAPH6 displayed a high steadiness against a wide spectrum of pH, temperature and salinity. The treatment of human HEK293 cells by different concentrations of BS-NAPH6 displayed that this latter exhibited no cytotoxic effect at concentrations below 200 µg/mL. Furthermore, the application of BS-NAPH6 in hydrocarbons removal from polluted soil, demonstrated that it was more efficient than tested chemical surfactants. Interestingly, BS-NAPH6 highlighted a significant healing activity on the wound site in a rat model compared to a commercial cream. Due to their attractive biological properties, strain NAPH6 as well as its biosurfactant BS-NAPH6 are considered as promising candidates for various applications including environmental and biomedical ones.
Dorra Hentati; Alif Chebbi; Asma Mahmoudi; Fatma Hadrich; Meriam Cheffi; Ilhem Frikha; Sami Sayadi; Mohamed Chamkha. Biodegradation of hydrocarbons and biosurfactants production by a newly halotolerant Pseudomonas sp. strain isolated from contaminated seawater. Biochemical Engineering Journal 2020, 166, 107861 .
AMA StyleDorra Hentati, Alif Chebbi, Asma Mahmoudi, Fatma Hadrich, Meriam Cheffi, Ilhem Frikha, Sami Sayadi, Mohamed Chamkha. Biodegradation of hydrocarbons and biosurfactants production by a newly halotolerant Pseudomonas sp. strain isolated from contaminated seawater. Biochemical Engineering Journal. 2020; 166 ():107861.
Chicago/Turabian StyleDorra Hentati; Alif Chebbi; Asma Mahmoudi; Fatma Hadrich; Meriam Cheffi; Ilhem Frikha; Sami Sayadi; Mohamed Chamkha. 2020. "Biodegradation of hydrocarbons and biosurfactants production by a newly halotolerant Pseudomonas sp. strain isolated from contaminated seawater." Biochemical Engineering Journal 166, no. : 107861.
In this paper, a comprehensive decision support tool based advanced monitoring system is developed to support transition to smart greenhouses for sustainable and clean food production. The decision framework aims to optimally control and manage the microclimate environments of smart connected greenhouses, where each greenhouse is defined as a self-water producing through an enhanced water desalination process. The main advantage of the current approach lies in the ability of the greenhouses to produce their water loads locally. This paper aims to develop an efficient decision tool able of performing specific monitoring and control functionalities to optimize the operation of the greenhouses where the aim is the energy and water savings. A decision model is implemented for the precise regulation and control of the indoor microclimate defining the optimal growth conditions for the crops. Furthermore, a predictive algorithm is developed to simulate in real time the operation of the greenhouses under various conditions, to assess the response of the system to storage dynamics and renewable sources, as well to control the complex indoor microclimate, energy and water flows, as well to optimize the crops growth. The developed tool is tested through a case study where the influences of climate data on the operation of the whole network are analyzed via numerical results.
Ahmed Ouammi; Oumaima Choukai; Driss Zejli; Sami Sayadi. A Decision Support Tool for the Optimal Monitoring of the Microclimate Environments of Connected Smart Greenhouses. IEEE Access 2020, 8, 212094 -212105.
AMA StyleAhmed Ouammi, Oumaima Choukai, Driss Zejli, Sami Sayadi. A Decision Support Tool for the Optimal Monitoring of the Microclimate Environments of Connected Smart Greenhouses. IEEE Access. 2020; 8 (99):212094-212105.
Chicago/Turabian StyleAhmed Ouammi; Oumaima Choukai; Driss Zejli; Sami Sayadi. 2020. "A Decision Support Tool for the Optimal Monitoring of the Microclimate Environments of Connected Smart Greenhouses." IEEE Access 8, no. 99: 212094-212105.
In the present study, the growth conditions and accumulation of ectoines (ectoine and hydroxyectoine) by Paludifilum halophilum DSM 102817T under salt stress conditions have been investigated. The productivity assay of this strain for ectoines revealed that the highest cellular content was reached in the minimal glucose sea water medium (SW-15) within 15% salinity. The addition of 0.1% (w/v) aspartic acid to the medium allowed an average of four times higher biomass production, and a dry mycelial biomass of 1.76 g L-1 was obtained after 6 days of growth in shake flasks at 40 °C and 200 rpm. Among the inorganic cations supplemented to the glucose SW-15 medium, the addition of 1 mM Fe2+ yielded the highest amount of mycelial biomass (3.45 g L-1) and total ectoines content (119 mg g-1), resulting in about 410 mg L-1 of products at the end of exponential growth phase. After 1 h of incubation in an osmotic downshock solution containing 2% NaCl, 70% of this content was released by the mycelium, and recovering cells maintained a high survival, with a maximal growth rate (µmax) of about 93% of the control population exposed to 15% NaCl. During growth at optimal salinity and temperature (15% NaCl and 40 °C), P. halophilum developed a compact and circular pellets that were easy to separate by simple decantation from both fermentation media and after hypoosmotic shock. Overall, the ectoines excreting P. halophilum could be a promising resource for ectoines production in a commercially valuable culture medium and at a large-scale fermentation process.
Houda Ayadi; Donyez Frikha-Dammak; Jawhar Fakhfakh; Mohamed Chamkha; Ilem Hassairi; Noureddine Allouche; Sami Sayadi; Sami Maalej. The saltern-derived Paludifilum halophilum DSM 102817T is a new high-yield ectoines producer in minimal medium and under salt stress conditions. 3 Biotech 2020, 10, 533 .
AMA StyleHouda Ayadi, Donyez Frikha-Dammak, Jawhar Fakhfakh, Mohamed Chamkha, Ilem Hassairi, Noureddine Allouche, Sami Sayadi, Sami Maalej. The saltern-derived Paludifilum halophilum DSM 102817T is a new high-yield ectoines producer in minimal medium and under salt stress conditions. 3 Biotech. 2020; 10 (12):533.
Chicago/Turabian StyleHouda Ayadi; Donyez Frikha-Dammak; Jawhar Fakhfakh; Mohamed Chamkha; Ilem Hassairi; Noureddine Allouche; Sami Sayadi; Sami Maalej. 2020. "The saltern-derived Paludifilum halophilum DSM 102817T is a new high-yield ectoines producer in minimal medium and under salt stress conditions." 3 Biotech 10, no. 12: 533.
This paper presents a greenhouse indoor environment controller based on model predictive control (MPC), which can be integrated into existing greenhouse regulatory systems to optimally maintain critical climatic variables, including artificial lighting levels, CO2 rate, indoor temperature and humidity level within acceptable limits. The MPC based optimization problem aims to maximize the rate of crop photosynthesis while optimizing the use of the available water and energy sources, taking into account the unpredictability and intermittent nature of renewable energies and external atmospheric conditions. This would facilitate the management of greenhouses by anticipating control actions for a better quality of crops production. For that, the mathematical formulation of the optimal control problem is presented, and the numerical results related to the application of the MPC to case studies are analyzed integrating the effects of greenhouse structural considerations and the influence of climate data on its operation.
Yasmine Achour; Ahmed Ouammi; Driss Zejli; Sami Sayadi. Supervisory Model Predictive Control for Optimal Operation of a Greenhouse Indoor Environment Coping With Food-Energy-Water Nexus. IEEE Access 2020, 8, 211562 -211575.
AMA StyleYasmine Achour, Ahmed Ouammi, Driss Zejli, Sami Sayadi. Supervisory Model Predictive Control for Optimal Operation of a Greenhouse Indoor Environment Coping With Food-Energy-Water Nexus. IEEE Access. 2020; 8 (99):211562-211575.
Chicago/Turabian StyleYasmine Achour; Ahmed Ouammi; Driss Zejli; Sami Sayadi. 2020. "Supervisory Model Predictive Control for Optimal Operation of a Greenhouse Indoor Environment Coping With Food-Energy-Water Nexus." IEEE Access 8, no. 99: 211562-211575.
Carotenoids have strong antioxidant activity as well as therapeutic value. Their production has been induced in algae under stressful culture conditions. However, the extreme culture conditions lead to the Programmed Cell Death (PCD) of algae, which affects their growth and productivity. This study was performed to evaluate the effect of salinity on the physiological and biochemical traits of Scenedesmus sp., thermal freshwater microalgae from Northern Tunisia. It was cultured under different NaCl concentrations ranging from 0 to 60 g/L. Results showed a good growth and high contents of total chlorophyll and carotenoids in Scenedesmus sp. cultured at 10 g/L of NaCl (salt-stressed 10 (Ss10)). The pigment composition of the Ss10 extract was acquired using HPLC–MS, and showed that the carotenoid fraction is particularly rich in xanthophylls. Moreover, the antioxidant (DPPH and FRAP) and enzymatic inhibition (tyrosinase and elastase) activities of the Ss10 extract were higher compared to those of the control culture. In addition, the cytotoxicity test on B16 cells showed that the Ss10 extract was non-toxic for all tested concentrations below 100 µg/mL. It also showed a rich unsaturated fatty acid (FA) composition. Therefore, these findings suggest that Scenedesmus sp. strain cultivated under mild stress salinity could be a source of biomolecules that have potential applications in the nutraceutical and cosmeceutical industries.
Wiem Elloumi; Ahlem Jebali; Amina Maalej; Mohamed Chamkha; Sami Sayadi. Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp. Biomolecules 2020, 10, 1515 .
AMA StyleWiem Elloumi, Ahlem Jebali, Amina Maalej, Mohamed Chamkha, Sami Sayadi. Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp. Biomolecules. 2020; 10 (11):1515.
Chicago/Turabian StyleWiem Elloumi; Ahlem Jebali; Amina Maalej; Mohamed Chamkha; Sami Sayadi. 2020. "Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp." Biomolecules 10, no. 11: 1515.
The chemical treatment of the wastewater used for the bioinsecticide production by the bacterium Photorhabdus temperata was investigated in this study. An improvement of the volatile suspended solids (VSS) solubilization along with an increase in protein, carbohydrate, reducing sugar and nitrogen concentrations were demonstrated after alkali and thermo-alkali hydrolysis. In contrast, the application of acidic and thermo-acidic pretreatments reduced the organic matter hydrolysis. Compared to untreated wastewater, the chemical oxygen demand (COD) solubilization and the heavy metal concentration, except manganese, were enhanced in all the chemically pretreated wastewaters. Although its low contribution in the solubilization of the wastewater organic matter, the acidic-pretreated wastewater showed the highest performance in supporting P. temperata biopesticide production. Indeed, using the acidic-pretreated wastewater as a fermentation medium decreased the lag phase, enhanced the growth of the strain K122 to reach a final biomass production of 20 × 108 cells/mL, increased culturable cell count to 262 × 106 cells/mL and improved oral toxicity against Ephestia kuehniella larvae by 68.4%. Among chemical pretreatments performed, the acidic hydrolysis was demonstrated to be the unique promising one for P. temperata bioinsecticide production due to its ability to reduce aromatic compounds as shown by Gas Chromatography-Mass Spectrometry (GC-MS) analysis.
Wafa Jallouli; Sahar Keskes; Wassim Guidara; Fatma Rezgui; Sami Sayadi; Slim Tounsi. Acidic pretreatment as a chemical approach for enhanced Photorhabdus temperata bioinsecticide production from industrial wastewater. Journal of Environmental Management 2020, 278, 111476 .
AMA StyleWafa Jallouli, Sahar Keskes, Wassim Guidara, Fatma Rezgui, Sami Sayadi, Slim Tounsi. Acidic pretreatment as a chemical approach for enhanced Photorhabdus temperata bioinsecticide production from industrial wastewater. Journal of Environmental Management. 2020; 278 ():111476.
Chicago/Turabian StyleWafa Jallouli; Sahar Keskes; Wassim Guidara; Fatma Rezgui; Sami Sayadi; Slim Tounsi. 2020. "Acidic pretreatment as a chemical approach for enhanced Photorhabdus temperata bioinsecticide production from industrial wastewater." Journal of Environmental Management 278, no. : 111476.
A halotolerant strain CO100 of Staphylococcus sp. was isolated from contaminated sediments taken from the fishing harbour of Sfax, Tunisia, as an efficient hydrocarbonoclastic candidate. Strain CO100 exhibited a high capacity to break down almost 72% of the aliphatic hydrocarbons contained in crude oil (1%, v/v), used as the sole carbon and energy source, after 20 days of culture, at 100 g/l NaCl, 37 °C and 180 rpm. The isolate CO100 displayed also its ability to grow on phenanthrene, fluoranthene and pyrene (100 mg/l), at 100 g/l NaCl. Moreover, the isolate CO100 showed a notable aptitude to synthesize an efficient tensioactive agent namely BS-CO100, on low-value substrates including residual frying oil and expired milk powder, thus reducing the high cost of biosurfactant production. The ESI/MS analysis designated that BS-CO100 belonged to lipopeptide class, in particular lichenysin and iturine members. Critical micelle concentrations of BS-CO100 were varying between 65 and 750 mg/l, depending on of the purity of the biosurfactant and the used carbon sources. BS-CO100 showed a high steadiness against a wide spectrum of pH (4.3–12), temperature (4–121 °C) and salinity (0–300 g/l NaCl), supporting its powerful tensioactive properties under various environmental conditions. Likewise, BS-CO100 exhibited no cytotoxic effect toward human HEK293 cells, at concentrations within 125 and 1000 μg/ml. Furthermore, the biosurfactant BS-CO100 exhibited remarkable anti-adhesive and anti-biofilm activities, being able to avoid and disrupt the biofilm formation by certain pathogenic microorganisms. In addition, BS-CO100 was found to have more potential to remove hydrocarbons from contaminated soils, compared to some chemical surfactants. In light of these promising findings, strain CO100, as well as its biosurfactant, could be successfully used in different biotechnological applications including the bioremediation of oil-polluted areas, even under saline conditions.
Dorra Hentati; Meriam Cheffi; Fatma Hadrich; Neila Makhloufi; Francesc Rabanal; Angeles Manresa; Sami Sayadi; Mohamed Chamkha. Investigation of halotolerant marine Staphylococcus sp. CO100, as a promising hydrocarbon-degrading and biosurfactant-producing bacterium, under saline conditions. Journal of Environmental Management 2020, 277, 111480 .
AMA StyleDorra Hentati, Meriam Cheffi, Fatma Hadrich, Neila Makhloufi, Francesc Rabanal, Angeles Manresa, Sami Sayadi, Mohamed Chamkha. Investigation of halotolerant marine Staphylococcus sp. CO100, as a promising hydrocarbon-degrading and biosurfactant-producing bacterium, under saline conditions. Journal of Environmental Management. 2020; 277 ():111480.
Chicago/Turabian StyleDorra Hentati; Meriam Cheffi; Fatma Hadrich; Neila Makhloufi; Francesc Rabanal; Angeles Manresa; Sami Sayadi; Mohamed Chamkha. 2020. "Investigation of halotolerant marine Staphylococcus sp. CO100, as a promising hydrocarbon-degrading and biosurfactant-producing bacterium, under saline conditions." Journal of Environmental Management 277, no. : 111480.
Olive groves are representative of the landscape and culture of Spain. They occupy 2.5 M ha (1.5 M ha in Andalusia) and are characterised by their multifunctionality. In recent years, socio-economic and environmental factors (i.e., erosion) have compromised their sustainability, leading farmers to abandon their farms or intensify their management. The main objective/purpose of this research was to study the drivers and concerns that condition farmers’ choice of a given olive grove management model. Taking the Estepa region as a case study (Andalusia, Spain), surveys were conducted among farmers with integrated and organic managed olive groves. The socio-economic aspects were the main objectives and concerns of the farmers with integrated olive groves. In the case of farmers with organic management, conservation objectives prevailed, and their concerns were oriented to environmental threats. The education level was a key factor in the adoption of given farm management, as it increased the level of environmental awareness. In the context of multifunctional agriculture, it would be desirable to increase this awareness of the environmental threats against olive groves, in order to provide incentives for the implementation of agri-environmental practices that would enhance the sustainability of these systems.
Antonio Alberto Rodríguez Sousa; Carlos Parra-López; Samir Sayadi-Gmada; Jesús M. Barandica; Alejandro J. Rescia. Evaluation of the Objectives and Concerns of Farmers to Apply Different Agricultural Managements in Olive Groves: The Case of Estepa Region (Southern, Spain). Land 2020, 9, 366 .
AMA StyleAntonio Alberto Rodríguez Sousa, Carlos Parra-López, Samir Sayadi-Gmada, Jesús M. Barandica, Alejandro J. Rescia. Evaluation of the Objectives and Concerns of Farmers to Apply Different Agricultural Managements in Olive Groves: The Case of Estepa Region (Southern, Spain). Land. 2020; 9 (10):366.
Chicago/Turabian StyleAntonio Alberto Rodríguez Sousa; Carlos Parra-López; Samir Sayadi-Gmada; Jesús M. Barandica; Alejandro J. Rescia. 2020. "Evaluation of the Objectives and Concerns of Farmers to Apply Different Agricultural Managements in Olive Groves: The Case of Estepa Region (Southern, Spain)." Land 9, no. 10: 366.