Typical successions in land use affect the dynamics of carbon (C) and nitrogen (N) in the soil. This study aimed to determine the effects of land use change on soil organic carbon and N content and stocks in pastures, crops, and forests in the Amazon. Soil C and N stocks were assessed at depths of 30 and 100 cm to determine ¹³C isotopic abundance. The concentrations of C and N in crops were lower (p < 0.05) than those in other land use types. Soil organic C and soil N stocks for pasture (67.6, 144.8, 5.7, and 13.3) and forest (77.1, 137.5, 6.3, and 13.8) systems were similar, but greater than those of the crop area (36.4, 63.9, 3.0, and 6.0), regardless of depth (30 and 100 cm for C and N). Land use change for pastures in the Arc of Deforestation region of the Amazon maintains SOC and N stocks in the soil and is more sustainable than the agricultural system with black pepper, as long as the conditions of soil, climate, and cultivation are similar. Part of the C3-derived carbon from the forest was replaced by C4-derived C from grasses at soil depths up to 100 cm.

Nitrous oxide (N₂O) is one of the main gases emitted from soils, and the changes in land use in the Amazon may alter gas emission patterns. The objective of this study was to evaluate the effects of land use, temperature, and nitrogen on N₂O emissions in soils in the Amazon. For this, three treatments randomized, with five repetitions, were incubated to quantify N₂O emissions: (i) three different land uses (wet rainforest, pasture, and agriculture); (ii) different temperatures (25, 30, 35, and 40 °C); and (iii) different nitrogen additions to the soil (0, 90, 180, and 270 kg of N ha⁻¹). Our results show that land use alters the flux of N₂O, with the highest emissions observed in agricultural soils compared to that in forest and pasture areas. The change in soil temperature to 30 °C increased N₂O emissions with land use, at which the emission of N₂O was higher in the pasture and agriculture soils. Our results showed that the emission of N₂O in the soil of the Amazon rainforest was low regardless of the temperature and nitrogen treatment. Therefore, the change in land use alters the resilience of the ecosystem, providing emissions of N₂O.

Nitrogen fertilization is one of the main management strategies for continuous pasture management with high productivity. We examined the effects of nitrogen fertilization on the morphogenic, structural, and tillering dynamic characteristics of Tanzania grass in the Amazon region in the state of Pará, Brazil. The study was conducted using a randomized block design with six treatments (0, 100, 200, 300, 400, and 500 kg N ha⁻¹ year⁻¹) and five replicates. The treatments were performed during the rainy season in 2016 and 2017 using urea as the nitrogen source. The leaf elongation rate was increased by 68.5% compared with that of the control treatment (p < 0.01). The leaf appearance rate and number of alive leaves increased with higher doses of nitrogen (p < 0.01, each). The regrowth period was reduced by approximately 13 days under 500 kg N ha⁻¹ (p < 0.01), thus providing more production cycles. Nitrogen fertilization was also associated with a higher tillering rate (p < 0.01); however, the rate of this increase decreased with increasing nitrogen dose. Higher nitrogen doses thus improved the development of Tanzania grass; however, this became less pronounced at doses < 300 kg N ha⁻¹.

The aim of the present study was to evaluate the effects of sowing depth and inoculation with Pseudomonas fluorescens on germination, emergence, shoot and root growth of Urochloa brizantha . A completely randomized design was used, in a 6 × 2 factorial arrangement, with 12 repetitions. The following sowing depths (SD) were evaluated: 0, 1, 2, 3, 6 and 12 cm; and the seed inoculation with P . fluorescens (I): with and without. Evaluations were carried out 25 days after plant emergence. No effects of the PS × I or I interaction were observed for all variables evaluated (P> 0.05). The germination and emergence percentages decreased linearly (P <0.05) as the SD increased. No plant emergence was observed at and at 12 cm depth. The morphometric characteristics of the plants (height, number of leaves, length of root and leaf blade width) decreased linearly with the increase of SD (P 0.05). However, an effect was observed on the shoot:root ratio, where plants sown more superficially had a greater relationship. The inoculation with Pseudomonas fluorescens in the seed, has no effect on the initial growth of Marandu grass. On the other hand, greater sowing depths affects negatively the initial growth.