Carbon dots (CDs) are a new type of carbon nanomaterial that have unique physical and chemical properties, good biocompatibility, low toxicity, and easy surface functionalization, making them widely used in biological imaging, environmental monitoring, chemical analysis, targeted drug delivery, disease diagnosis, therapy, etc. In this review, our content is mainly divided into four parts. In the first part, we focused on the preparation methods of CDs, including arc discharge, laser ablation, electrochemical oxidation, chemical oxidation, combustion, hydrothermal/solvent thermal, microwave, template, method etc. Next, we summarized methods of CD modification, including heteroatom doping and surface functionalization. Then, we discussed the optical properties of CDs (ultraviolet absorption, photoluminescence, up-conversion fluorescence, etc.). Lastly, we reviewed the common applications of CDs in biomedicine from the aspects of in vivo and in vitro imaging, sensors, drug delivery, cancer theranostics, etc. Furthermore, we also discussed the existing problems and the future development direction of CDs.

Actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1), a long noncoding RNA, is significantly highly expressed and associated with metastasis and poor prognosis in many cancers, including nasopharyngeal carcinoma (NPC). In this study, we aim to identify the role of AFAP1-AS1 acting as an oncogenic lncRNA to promote NPC metastasis. The role of AFAP1-AS1, miR-423-5p, and FOSL2 in NPC metastasis was investigated in vitro and in vivo. Bioinformatics analysis and luciferase activity assays were used to identify the interaction between AFAP1-AS1, miR-423-5p, and FOSL2. Additionally, real-time PCR and western blotting were used to assess the function of AFAP1-AS1 acting as an oncogenic lncRNA to promote NPC progression by regulating miR-423-5p and the downstream Rho/Rac pathway. In this study, we determined that AFAP1-AS1 functions as a competing endogenous RNA in NPC to regulate the Rho/Rac pathway through miR-423-5p. These interactions can mediate the expression of RAB11B, LASP1, and FOSL2 and accelerate cell migration and invasion via the Rho/Rac signaling pathway or FOSL2. AFAP1-AS1 and FOSL2 could competitively bind with miR-423-5p to regulate several molecules, including RAB11B and LASP1 of the Rho/Rac signaling pathway. AFAP1-AS1 can also regulate the expression of LASP1, which was transcriptionally regulated by FOSL2, resulting in increased migration and invasion of NPC cells via the Rho/Rac signaling pathway. The observations in this study identify an important role for AFAP1-AS1 as a competing endogenous RNA (ceRNA) in NPC pathogenesis and indicate that it may serve as a potential target for cancer diagnosis and treatment.

In this research, we successfully developed a green, economical and effective one–step hydrothermal method for the synthesis of fluorescent nitrogen–doped carbon dots (N–CDs) by utilizing fresh tea leaves and urea as the carbon and nitrogen sources, respectively. The obtained N–CDs were characterized by TEM, XPS and FT–IR. We found that the N–CDs were near–spherical with an average size of about 2.32 nm, and contained abundant oxygen and nitrogen functional groups. The N–CDs exhibited bright blue fluorescence under ultraviolet illumination, with the maximum emission at 455 nm. Meanwhile, the as–prepared N–CDs could be selectively quenched by Fe³⁺ ions. The quenching of N–CDs is linearly correlated with the concentration of Fe³⁺ in the range of 0.1–400 μM with a low detection limit of 0.079 μM. Significantly, the N–CDs present excellent biocompatibility and high photostability. The results also depict that multicolor fluorescence is displayed under a fluorescence microscope and successfully applied for the detection of intracellular Fe³⁺. To sum up, the fluorescent N–CDs are expected to be a sensitive detection probe for Fe³⁺ in biological systems.