Analysis of pollen material obtained from the Hirst-type apparatus, which is a tedious and labor-intensive process, is usually performed by hand under a microscope by specialists in palynology. This research evaluated the automatic analysis of pollen material performed based on digital microscopic photos. A deep neural network called YOLO was used to analyze microscopic images containing the reference grains of three taxa typical of Central and Eastern Europe. YOLO networks perform recognition and detection; hence, there is no need to segment the image before classification. The obtained results were compared to other deep learning object detection methods, i.e., Faster R-CNN and RetinaNet. YOLO outperformed the other methods, as it gave the mean average precision (mAP@.5:.95) between 86.8% and 92.4% for the test sets included in the study. Among the difficulties related to the correct classification of the research material, the following should be noted: significant similarities of the grains of the analyzed taxa, the possibility of their simultaneous occurrence in one image, and mutual overlapping of objects.
This study investigates Weibull distribution modeling for data under grouped observations. Two data grouping methods (equal-width and optimal) were compared for estimating parameters of the Weibull distribution using maximum likelihood estimation (MLE) in each case. Methodologically, our contribution is twofold: First, we derive the correct Fisher information matrix for grouped data in the two-parameter Weibull and use it to compute optimal interval boundaries. Second, we derive maximum likelihood estimators for data grouped under these optimal intervals. The fit of the assumed distributions was evaluated using chi-squared goodness-of-fit tests. We also calculated Asymptotic Relative Efficiency (ARE) to compare the precision of parameter estimates across different grouping approaches. Optimal boundaries yielded systematically higher ARE than equal-width grouping in 100% of comparisons for the shape parameter . Gains for the scale parameter were smaller and occurred in about 62% of cases. Optimal grouping also produced generally higher chi-squared goodness-of-fit p-values than equal-width grouping, indicating a better fit. From a symmetry standpoint, the Weibull distribution is inherently asymmetric, with the degree of asymmetry governed by the shape parameter . We show that the choice of grouping affects the estimate of and, thus, the inferred skewness, further explaining why optimally designed intervals yield both higher precision and a more faithful representation of failure behavior.