Plasma medicine is a relatively new field that investigates potential applications of cold atmospheric-pressure plasmas in bioengineering, such as for bacterial inactivation and degradation of organic molecules in water. In order to enunciate mechanisms of bacterial inactivation at molecular or atomic levels, we investigated the interaction of atmospheric-pressure air microplasmas with amino acids in aqueous solution by using high-resolution mass spectrometry (HRMS). Results show that the oxidation effect of plasma-induced species on the side chains of the amino acids can be categorized into four types, namely hydroxylation, nitration, dehydrogenation and dimerization. In addition, relative activities of amino acids resulting from plasma treatment come in descending order as follows: sulfur-containing carbon-chain amino acids > aromatic amino acids > five-membered ring amino acids > basic carbon-chain amino acids. Since amino acids are building blocks of proteins vital to the growth and reproduction of bacteria, these results provide an insight into the mechanism of bacterial inactivation by plasma.

Atmospheric-pressure N ₂ , He, air, and O ₂ microplasma arrays have been used to inactivate Escherichia coli cells suspended in aqueous solution. Measurements show that the efficiency of inactivation of E. coli cells is strongly dependent on the feed gases used, the plasma treatment time, and the discharge power. Compared to atmospheric-pressure N ₂ and He microplasma arrays, air and O ₂ microplasma arrays may be utilized to more efficiently kill E. coli cells in aqueous solution. The efficiencies of inactivation of E. coli cells in water can be well described by using the chemical reaction rate model, where reactive oxygen species play a crucial role in the inactivation process. Analysis indicates that plasma-generated reactive species can react with E. coli cells in water by direct or indirect interactions.

We report the formation of wave-like structures and nanostructured fuzzes in the polycrystalline tungsten (W) irradiated with high-flux and low-energy helium (He) ions. From conductive atomic force microscope measurements, we have simultaneously obtained the surface topography and current emission images of the irradiated W materials. Our measurements show that He-enriched and nanostructured strips are formed in W crystal grains when they are exposed to low-energy and high-flux He ions at a temperature of 1400 K. The experimental measurements are confirmed by theoretical calculations, where He atoms in W crystal grains are found to cluster in a close-packed arrangement between {101} planes and form He-enriched strips. The formations of wave-like structures and nanostructured fuzzes on the W surface can be attributed to the surface sputtering and swelling of He-enriched strips, respectively.

Recently, plasma-activated water (PAW) has received extensive attention in food safety field due to its advantages of eco-friendly and high sterilization efficiency. In this paper, the effects of PAW on microbe loads and quality of fresh-cut kiwifruit (FCK) were studied, and the preservation mechanism of PAW on FCK was explored. A 1 mL of PAW and 1 mL of sterile water as control sample were separately sprayed on FCK and stored at 4 °C for 8 days. Compared to the control sample, the microbial population of FCK was reduced by approximately 1.8 log CFU/g after the PAW treatment. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in PAW-treated FCK samples were improved. Further test results showed that H₂O₂ and O₂⁻ accumulation rates of PAW-treated FCK were slower than that of the control sample. Finally, by analyzing the effect of pH and H₂O₂ on the activity of antioxidant enzymes in FCK, it was found that either pH or H₂O₂ treatment could enhance the activities of antioxidant enzymes in FCK. Concerning the fruit quality, our measurement shows that the PAW treatment had no significant effect on the firmness and b* value of FCK.