Copper-based bactericides have appeared as a new tool in crop protection and offer an effective solution to combat bacterial resistance. In this work, two copper nanoparticle products that were previously synthesized and evaluated against major bacterial and fungal pathogens were tested on their ability to control the bacterial spot disease of tomato. Growth of Xanthomonas campestris pv. vesicatoria, the causal agent of the disease, was significantly suppressed by both nanoparticles, which had superior function compared to conventional commercial formulations of copper. X-ray fluorescence spectrometry measurements in tomato leaves revealed that bioavailability of copper is superior in the case of nanoparticles compared to conventional formulations and is dependent on synthesis rather than size. This is the first report correlating bioavailability of copper to nanoparticle efficacy.

Monitoring the progress of fracture healing is essential in order to establish the appropriate timing that ensures adequate bone strength for weight-bearing. In the present experimental study on a rat model of femoral fracture healing, the measurement of bone density and strength by peripheral quantitative computerized tomography (pQCT) was correlated with the modal damping factor (MDF) method. Four groups of 12 male six-month-old Wistar rats each were anesthetized and submitted to baseline femoral pQCT and MDF scanning, followed by aseptic midshaft osteotomy of the right femur which was fixed by a locking intramedullary nail technique. The animals were left to recover and re-scanned following euthanasia of each group after six, eight, 10, and 12 weeks, respectively. The parameters measured by the pQCT method were total bone mineral density (BMD) and polar strength strain index (SSIp). Fracture healing progressed over time and at 12 weeks post-osteotomy there was no statistically significant difference between the osteotomized right and the control left femurs regarding MDF, BMD, and SSIp measurements. The highest correlations for the osteotomized femurs were observed between MDF and BMD (r = -0.647, P = 0.043), and between MDF and SSIp (r = -0.350, P = 0.321), at 10 weeks postoperatively. The high to moderate correlations between MDF and BMD, and between MDF and SSIp respectively, support the validity of MDF in assessing fracture healing. Based on our findings in this fracture healing animal model, the results from the MDF method are reliable and correlate highly with the total BMD and moderately with the SSI polar values obtained by the pQCT method of bone quality measurement. Further studies are needed which may additionally support that the MDF method can be an attractive portable alternative to monitor fracture healing in the community.

Climate change and extreme events such as droughts, heavy rainfall and flooding can influence the water column stratification in reservoir dams, decrease storage capacity, increase sediment and pollutant loads and, as a result, affect water quality. The seasonal variation in the water column stratification of reservoirs is an important parameter for the study of dam life cycle as well as water management and use. In the present study a detailed bathymetric survey was carried out, and a digital elevation model (DEM) of the reservoir was constructed. Seasonal physicochemical monitoring data such as temperature, dissolved oxygen, pH and conductivity are presented. The seasonal thermal stratification was recorded, resulting in an isolated hypolimnion where anoxic layers formed below 17 m in summer and autumn. Manganese and iron concentrations exhibited values higher than 150 mg/L in the anoxic hypolimnion during summer and autumn, indicating solubilization from the sediment. The observed seasonal and depth-dependent variations in physicochemical parameters underline the reservoir’s susceptibility to eutrophication and metal mobilization, particularly during stratified periods. These findings are critical for designing management strategies to mitigate potential water quality issues.