Flow cytometry technique (FC) is a standard diagnostic tool for diagnostics of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) assessing the immunophenotype of blast cells. BCP-ALL is often associated with underlying genetic aberrations, that have evidenced prognostic significance and can impact the disease outcome. Since the determination of patient prognosis is already important at the initial phase of BCP-ALL diagnostics, we aimed to reveal specific genetic aberrations by finding specific multiple antigen expression patterns with FC immunophenotyping. The FC immunophenotype data were analysed using machine learning methods (gradient boosting, decision trees, classification rules). The obtained results were verified with the use of repeated cross-validation. The t(12;21)/ETV6-RUNX1 aberration occurs more often when blasts present high expression of CD10, CD38, low CD34, CD45 and specific low expression of CD81. The t(v;11q23)/KMT2A is associated with positive NG2 expression and low CD10, CD34, TdT and CD24. Hyperdiploidy is associated with CD123, CD66c and CD34 expression on blast cells. In turn, high expression of CD81, low expression of CD45, CD22 and lack of CD123 and NG2 indicates that none of the studied aberrations is present. Detecting aberrations in pediatric BCP-ALL, based on the expression of multiple markers, can be done with decent efficiency.

It seems to be a truism to say that we should pay more and more attention to network traffic safety. Such a goal may be achieved with many different approaches. In this paper, we put our attention on the increase in network traffic safety based on the continuous monitoring of network traffic statistics and detecting possible anomalies in the network traffic description. The developed solution, called the anomaly detection module, is mostly dedicated to public institutions as the additional component of the network security services. Despite the use of well-known anomaly detection methods, the novelty of the module is based on providing an exhaustive strategy of selecting the best combination of models as well as tuning the models in a much faster offline mode. It is worth emphasizing that combined models were able to achieve 100% balanced accuracy level of specific attack detection.

The European Survey on Income and Living Conditions (EU-SILC) is the basic source of information published by CSO (the Central Statistical Office of Poland) about the relative poverty indicator, both for the country as a whole and at the regional level (NUTS 1). Estimates at lower levels of the territorial division than regions (NUTS 1) or provinces (NUTS 2, also called ’voivodships’) have not been published so far. These estimates can be calculated by means of indirect estimation methods, which rely on information from outside the subpopulation of interest, which usually increases estimation precision. The main aim of this paper is to show results of estimation of the poverty indicator at a lower level of spatial aggregation than the one used so far, that is at the level of subregions in Poland (NUTS 3) using the small area estimation methodology (SAE), i.e. a model–based technique – the EBLUP estimator based on the Fay–Herriot model. By optimally choosing covariates derived from sources unaffected by random errors we can obtain results with adequate precision. A territorial analysis of the scope of poverty in Poland at NUTS 3 level will be also presented in detail⁴. The article extends the approach presented by Wawrowski (2014).