Given the importance of wood in many industrial applications, much research has focused on wood formation, especially lignin biosynthesis. However, the mechanisms governing the regulation of lignin biosynthesis in the rubber tree (Hevea brasiliensis) remain to be elucidated. Here, we gained insight into the mechanisms of rubber tree lignin biosynthesis using reaction wood (wood with abnormal tissue structure induced by gravity or artificial mechanical treatment) as an experimental model. We performed transcriptome analysis of rubber tree mature xylem from tension wood (TW), opposite wood (OW), and normal wood (NW) using RNA sequencing (RNA-seq). A total of 214, 1,280, and 32 differentially expressed genes (DEGs) were identified in TW vs. NW, OW vs. NW, and TW vs. OW, respectively. GO and KEGG enrichment analysis of DEGs from different comparison groups showed that zeatin biosynthesis, plant hormone signal transduction, phenylpropanoid biosynthesis, and plant–pathogen interaction pathways may play important roles in reaction wood formation. Sixteen transcripts involved in phenylpropanoid biosynthesis and 129 transcripts encoding transcription factors (TFs) were used to construct a TF–gene regulatory network for rubber tree lignin biosynthesis. Among them, MYB, C2H2, and NAC TFs could regulate all the DEGs involved in phenylpropanoid biosynthesis. Overall, this study identified candidate genes and TFs likely involved in phenylpropanoid biosynthesis and provides novel insights into the mechanisms regulating rubber tree lignin biosynthesis.

Litter and soil play an important role in influencing hydrological processes and the global water cycle. Artificial afforestation, as a part of vegetation restoration, was constructed in the gully erosion areas of latosol with the objective to prevent erosion. Variations in the hydrological properties in soils that have undergone vegetation restoration from gully erosion are not well understood. In this study, we examine the variations in the litter thickness and mass, soil structure and porosity, and hydrological properties of four forest types (eucalyptus–grass forest, bamboo–grass forest, acacia–grass forest, and shrub–grass forest). The results show that the total litter thickness varied from 1.71 to 3.74 cm and was highest in the acacia–grass forest. The total litter mass for the acacia–grass forest, 3.49 ± 0.06 t·ha⁻¹, was significantly higher than that for the other forest types. The mass of the undecomposed litter (UL) layer was significantly lower than that of the semi-decomposed litter (SL). (2) The maximum water-retention capacity (W_max) and effective water-retention capacity (W_eff) of the SL layer were greater than those of the UL layer. The W_max and W_eff for the acacia–grass forest were markedly larger than those of the eucalyptus–grass, bamboo–grass, and shrub–grass forests. The water absorption rates of the SL and UL layers were highest during the onset of the immersion experiment, declined exponentially with time, and declined rapidly in the first 2 h. (4) The soil bulk density ranged from 1.46 g·cm⁻³ to 1.54 g·cm⁻³, and the total porosity ranged from 32.06% to 37.13%. The soil bulk density increased with the increasing soil depth, while the total porosity decreased gradually. The soil water-holding capacity of the soil layer of 0–60 cm in the acacia–grass forest (301.76 t·ha⁻¹) was greater than that of the other forest types. A comprehensive evaluation of the water conservation capacity by the entropy weight method showed that the water conservation capacity was greatest in the acacia–grass forest. The higher water-holding capacity of the acacia–grass forest may be more effective in enhancing rainfall interception, minimizing splash erosion, and decreasing surface runoff. Here, the results indicate that acacia–grass forest restoration can mitigate soil erosion by favoring soil and water conservation, improving the environment in the gully erosion area of latosol.

Litter is one of the key components of the forest ecosystem and plays a role as the second active layer influencing hydrological processes, which has affected the global water cycle. Soil- and water-conservation forests were constructed by artificial afforestation as a part of vegetation restoration in the eroded area of Latosol, and little is known about the differences in the hydrological properties of vegetation restoration in the eroded area of Latosol in the tropical region. We investigated the litter thickness, mass, and hydrological properties in three soil- and water-conservation forests (Eucalyptus robusta, Hevea brasiliensis, and Acacia mangium) through in situ surveys and laboratory experiments. The results showed that (1) the total litter thickness varied from 2.16 to 5.53 cm and was highest in the A. mangium forest. The total litter mass for A. mangium, 14.66 ± 1.09 t·ha⁻¹, was significantly higher than that for E. robusta (5.45 ± 0.59 t·ha⁻¹) and H. brasiliensis (3.01 ± 0.14 t·ha⁻¹). The mass of the semi-decomposed litter (SDL) layer was markedly higher than that of the un-decomposed litter (UDL) layer. (2) The maximum water-retention capacity (W_max) and effective water-retention capacity (W_eff) of the SDL layer were larger than the UDL layer for three forest plantations. The W_max and W_eff for the A. mangium stand were significantly higher than those for the E. robusta and H. brasiliensis stand. (3) The water-absorption rate of the SDL and UDL layer were highest at the onset of the immersion experiment, declined exponentially with time, and especially declined rapidly in the first 2 h. A higher water-holding capacity of A. mangium may be more effective in enhancing rainfall interception, minimizing splash erosion, and decreasing surface runoff. These results indicate that planting A. mangium in E. robusta and H. brasiliensis forests and then turning them into mixed forests should improve soil and water conservation and maximize their ecological benefits.

Expected changes in precipitation over large regions of the world under global climate change will have profound effects on terrestrial ecosystems in arid and semiarid regions. To explore how changes in the amount of precipitation in the growing season would affect soil nitrogen (N) availability in a semiarid ecosystem, we established rainout shelters and irrigation systems by simulating 30% reduced (DRY) and 30% increased precipitation (WET) relative to natural precipitation (Control) to measure some key soil process properties for two growing seasons in a nutrient-poor Mongolian pine (P. sylvestris var. mongolica) plantation. Both WET and DRY treatments significantly affected monthly soil inorganic nitrogen concentrations, which showed a higher inorganic N under DRY than Control in each month and lower in WET than Control. Monthly soil microbial biomass N content was reduced by DRY and raised by WET treatments. The results indicated the asynchrony of the availability of soil moisture and soil nutrients in Mongolian pine plantations at the Horqin Sandy Lands in Northeast China. Water limited plant growth in Mongolian pine plantations when precipitation decreased, and nitrogen limitation became increasingly important when precipitation increased. Accumulation of N in microbial biomass is an important mechanism for N cycling in this ecosystem. To effectively manage Mongolian pine plantations, it is advised that evapotranspiration is minimized when precipitation decreases and that there is an increase in soil N availability by protecting litterfall when precipitation increases.

The water conservation capacity of the litter and soil layers of forest ecosystems improves the function of forest ecosystems in conserving soil and water. Plantation restoration plays a key role in preventing soil erosion. In order to evaluate the water conservation capacity of plantation restoration in Latosol soil-eroded region, we analyzed the litter thickness and mass, water absorption process, water holding recovery process, and soil water holding capacity of five restoration types (Hevea brasiliensis, Acacia mangium, Eucalyptus robusta, Acacia–Eucalyptus, and Acacia–Hevea) in the Mahuangling Watershed, Hainan province. The results showed that the thickness of the litter ranged from approximately 3.42 ± 0.24 to 4.73 ± 0.81 cm, and the litter mass ranged from 5.04 ± 1.52 t·ha⁻¹ to 13.16 ± 1.76 t·ha⁻¹, with higher litter mass in the SL layer than in the UL layer. The litter mass of A. mangium was higher than that of H. brasiliensis, E. robusta, Acacia–Eucalyptus, and Acacia–Hevea, which was 3.16 ± 1.76 t·ha⁻¹. A. mangium forest was significantly higher than other plantation restoration types in terms of the maximum water retention capacity (Q_max) and the effective water retention capacity (Q_eff). The soil bulk weight ranged from approximately 1.52 ± 0.09 to 1.59 ± 0.08 g·cm⁻³, and porosity ranged from 31.77 ± 4.72 to 35.62 ± 3.02%, both of which increased with the depth of the soil layer. The water holding capacity of 0–60 cm soil varied from approximately 12.94 ± 7.91 to 45.02 ± 31.79 t·ha⁻¹, with A. mangium having the best soil permeability and the strongest soil water holding capacity. The entropy weight method was used to conduct a comprehensive evaluation, and the results showed that the water conservation capacity of the soil layer was 1.26 times higher than that of the litter layer, in which the water conservation capacity of A. mangium was the strongest, with a comprehensive evaluation value of 0.2854, which effectively intercepted rainfall and reduced surface runoff. Hence, we suggest that the planting of A. mangium should be considered in future ecological restoration projects of the erosion area of Mahuangling in order to improve the function of conserving soil and water in a restoration forest ecosystem.