White Analytical Chemistry (WAC) provides a holistic framework for evaluating analytical methods by balancing analytical performance, environmental sustainability, and practical efficiency. Existing WAC assessment tools offer structured evaluation but often lack flexibility or comprehensiveness. To bridge this gap, we introduce the Whiteness Evaluation for Chemical Analysis (WECA) tool as a dynamic, web-based application that enables customizable, context-aware assessment of analytical methods. WECA allows users to select 2–4 criteria per RGB domain (Red: analytical performance; Green: environmental impact; Blue: practical efficiency), assign user-defined weights, and visualize results through an intuitive color-coded interface. The tool calculates a composite WECA score (%) that reflects overall method “whiteness”. Three case studies, covering HPLC-DAD, micellar electrokinetic chromatography, and electrochemical sensing, demonstrate WECA’s applicability and its ability to highlight method strengths and weaknesses across diverse analytical scenarios. WECA represents a step toward more adaptable, transparent, and visually intuitive method evaluation in alignment with the evolving principles of WAC.

A completely new direct voltammetric method has been developed for determination of acetaminophen (APAP), known as popular analgesic drug. The present electroanalytical method is based on anodic oxidation of APAP at the glassy carbon paste electrode modified with reduced graphene oxide (RGO). Key experimental conditions were studied, resulting in a set of optimal conditions: acetate buffer (pH 5.0) as working medium electrolyte, content of RGO, parameters of squarewave voltammetry including the potential step of 5 mV, potential amplitude of 50 mV, and frequency of 40 Hz. If peak area is used for evaluation, a linear range from 1.2 × 10^–6to 2.2 × 10^–4 mol L⁻¹characterized by determination coefficient of 0.9971, limits of quantification and detection, 9.3 × 10^–7 mol L⁻¹and 3.1 × 10^–7 mol L⁻¹, respectively, will be obtained. Under validation, the precision was described by relative standard deviation of 2.9% for the model sample analysis. Finally, the developed voltammetric method was compared with a reference high-performance liquid chromatography method in the analysis of commercially available pharmaceutical preparation and human urine collected from five healthy volunteers.Graphical abstract

In this study, a new, rapid, simple, and cost-effective fluorescent probe based on carbon dots (CDs) has been developed for the selective determination of nitrazepam (NZP). The CDs were synthesized with high production yield (71.43%) through one step carbonization of dried banana peels. The fluorescence intensity of the CDs is quenched by increasing the NZP concentration as a result of the inner-filter effect and dynamic quenching mechanism. This fluorescent sensor effectively quantified NZP in the linear range of 0.30–26 μg mL⁻¹, with a limit of detection of 0.10 μg mL⁻¹. The performance of the sensor was validated according to ICH guidelines and exhibited high precision and accuracy. The proposed method was also successfully extended for determining NZP in tablet dosage form and milk samples.